公开(公告)号：US20150268235A1

公开(公告)日：2015-09-24

申请号：US14731740

申请日：2015-06-05

Applicant: Advanced Liquid Logic, Inc.

Inventor： Michael G. Pollack ,  Vamsee K. Pamula ,  Ramakrishna Sista ,  Arjun Sudarsan

IPC: G01N33/543

CPC classification number: G01N33/54333 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502715 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0442 ,  B03C1/02 ,  B03C1/288 ,  B03C1/30 ,  B03C5/005 ,  B03C2201/18 ,  B03C2201/26 ,  C12N15/1013 ,  G01N33/561 ,  G01N2035/1046 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

Abstract: The invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay.

Abstract translation: 本发明涉及在液滴致动器上的珠孵化和洗涤。 提供了用于将用一抗标记的磁响应珠，样品（即分析物）和二次报告基因抗体孵育在磁体上，磁铁上和从磁体上离开并完全离开磁体的方法。 还提供了使用形状辅助的液滴合并来洗涤磁响应珠的方法。 还提供了用于形状介导的分离，输送和分配含有磁响应珠的样品液滴的方法。 本发明的装置和方法提供了免疫测定中分析物的快速结果和最佳检测的时间。

公开(公告)号：US20150212043A1

公开(公告)日：2015-07-30

申请号：US14428219

申请日：2013-10-14

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Michael G. Pollack

IPC: G01N27/447

CPC classification number: G01N27/44791 ,  B01L3/0241 ,  B01L3/502792 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/088 ,  B01L2300/0883 ,  B01L2300/1827 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0457 ,  G01N27/44743

Abstract: A liquid handling system for supplying liquids to a flow cell (FC). The system may include a droplet actuator cartridge, wherein the droplet actuator and a flow cell are fluidly coupled to, or situated within, a droplet operations gap of the droplet actuator.

Abstract translation: 一种用于向流动池（FC）供应液体的液体处理系统。 该系统可以包括液滴致动器盒，其中液滴致动器和流动池流体耦合到液滴致动器的液滴操作间隙或位于液滴致动器的液滴操作间隙内。

公开(公告)号：US09086345B2

公开(公告)日：2015-07-21

申请号：US14308110

申请日：2014-06-18

Applicant: Advanced Liquid Logic, Inc.

Inventor： Ramakrishna Sista ,  Vamsee K. Pamula ,  Vijay Srinivasan ,  Michael G. Pollack ,  Allen Eckhardt

IPC: G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  G01N1/38 ,  B01F11/00 ,  B01F13/00 ,  B01L3/00 ,  B01L7/00 ,  G01N35/10

CPC classification number: G01N33/54326 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0809 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502792 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2300/06 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0442 ,  G01N1/38 ,  G01N2001/386 ,  G01N2035/1046 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

Abstract: The invention provides a method of dispersing or circulating magnetically responsive beads within a droplet in a droplet actuator. The invention, in one embodiment, makes use of a droplet actuator with a plurality of droplet operations electrodes configured to transport the droplet, and a magnetic field present at a portion of the plurality of droplet operations electrodes. A bead-containing droplet is provided on the droplet actuator in the presence of the uniform magnetic field. Beads are circulated in the droplet during incubation by conducting droplet operations on the droplet within a uniform region of the magnate field. Other embodiments are also provided.

Abstract translation: 本发明提供了一种在液滴致动器中的液滴内分散或循环磁响应珠的方法。 在一个实施例中，本发明利用具有配置成传送液滴的多个液滴操作电极的液滴致动器和存在于多个液滴操作电极的一部分处的磁场。 在均匀磁场存在的情况下，在液滴致动器上提供含珠的液滴。 在孵育期间，通过在大气场的均匀区域内的液滴上进行液滴操作，珠子在液滴中循环。 还提供了其他实施例。

公开(公告)号：US20150053562A1

公开(公告)日：2015-02-26

申请号：US14526928

申请日：2014-10-29

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Michael G. Pollack ,  Vamsee K. Pamula ,  Vijay Srinivasan

IPC: G01N27/447

CPC classification number: G01N27/447 ,  B01L3/502761 ,  B01L3/502792 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2400/0427 ,  C12Q1/6806 ,  G01N15/1031 ,  G01N27/44717 ,  G01N27/44721 ,  G01N33/543 ,  G01N35/10 ,  G01N2015/0065 ,  G01N2015/0088 ,  G01N2015/1081 ,  G01N2035/1034 ,  Y10T436/11 ,  Y10T436/118339 ,  Y10T436/25 ,  Y10T436/2575

Abstract: The present invention relates to droplet-based particle sorting. According to one embodiment, a droplet microactuator is provided and includes: (a) a suspension of particles; and (b) electrodes arranged for conducting droplet operations using droplets comprising particles. A method of transporting a particle is also provided, wherein the method includes providing a droplet comprising the particle and transporting the droplet on a droplet microactuator.

Abstract translation: 本发明涉及基于液滴的颗粒分选。 根据一个实施例，提供了液滴微致动器，其包括：（a）颗粒悬浮液; 和（b）布置成使用包含颗粒的液滴进行液滴操作的电极。 还提供了输送颗粒的方法，其中所述方法包括提供包含颗粒的液滴并将液滴输送到液滴微致动器上。

公开(公告)号：US20150038344A1

公开(公告)日：2015-02-05

申请号：US14520736

申请日：2014-10-22

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Michael G. Pollack ,  Allen E. Eckhardt ,  Prasanna Thwar ,  Jeremy Rouse

IPC: C12N15/10

CPC classification number: C12N15/1075 ,  B01J19/0046 ,  C12N15/1006 ,  C40B40/08 ,  C40B50/06

Abstract: A method of preparing a nucleic acid library in droplets in contact with oil, including: (a) blunt-ending nucleic acid fragments in a droplet in the oil to yield blunt-ended nucleic acid fragments; (b) phosphorylating the blunt-ended nucleic acid fragments in a droplet in the oil to yield phosphorylated nucleic acid fragments; coupling A-tails to the phosphorylated nucleic acid fragments in a droplet in the oil to yield A-tailed nucleic acid fragments; and (d) coupling nucleic acid adapters to the A-tailed nucleic acid fragments in a droplet in the oil to yield the nucleic acid library comprising adapter-ligated nucleic acid fragments.

Abstract translation: 制备与油接触的液滴核酸文库的方法，包括：（a）在油中的液滴中的平端核酸片段以产生平端核酸片段; （b）使油中的液滴中的平端核酸片段磷酸化以产生磷酸化的核酸片段; 将A尾连接到油中的液滴中的磷酸化核酸片段以产生A尾核酸片段; 和（d）将核酸衔接子偶联到油中的液滴中的A尾核酸片段，以产生包含衔接子连接的核酸片段的核酸文库。

公开(公告)号：US20140329307A1

公开(公告)日：2014-11-06

申请号：US14290057

申请日：2014-05-29

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Alexander Shenderov ,  Michael G. Pollack

IPC: B01L7/00

CPC classification number: B01L7/525 ,  B01L3/502792 ,  B01L2200/0673 ,  B01L2300/0654 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/1816 ,  B01L2300/1827 ,  B01L2300/1872 ,  B01L2400/0427

Abstract: Methods and devices for conducting chemical or biochemical reactions that require multiple reaction temperatures are described. The methods involve moving one or more reaction droplets or reaction volumes through various reaction zones having different temperatures on a microfluidics apparatus. The devices comprise a microfluidics apparatus comprising appropriate actuators capable of moving reaction droplets or reaction volumes through the various reaction zones.

Abstract translation: 描述了需要多个反应温度的用于进行化学或生化反应的方法和装置。 所述方法包括在微流体装置上移动一个或多个反应液滴或反应体积通过具有不同温度的各种反应区。 这些装置包括微流体装置，其包括能够通过各种反应区移动反应液滴或反应体积的适当致动器。

公开(公告)号：US20140302580A1

公开(公告)日：2014-10-09

申请号：US14308110

申请日：2014-06-18

Applicant: Advanced Liquid Logic, Inc.

Inventor： Ramakrishna Sista ,  Vamsee K. Pamula ,  Vijay Srinivasan ,  Michael G. Pollack ,  Allen Eckhardt

IPC: G01N1/38

CPC classification number: G01N33/54326 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0809 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502792 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2300/06 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0442 ,  G01N1/38 ,  G01N2001/386 ,  G01N2035/1046 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

Abstract: The invention provides a method of dispersing or circulating magnetically responsive beads within a droplet in a droplet actuator. The invention, in one embodiment, makes use of a droplet actuator with a plurality of droplet operations electrodes configured to transport the droplet, and a magnetic field present at a portion of the plurality of droplet operations electrodes. A bead-containing droplet is provided on the droplet actuator in the presence of the uniform magnetic field. Beads are circulated in the droplet during incubation by conducting droplet operations on the droplet within a uniform region of the magnate field. Other embodiments are also provided.

Abstract translation: 本发明提供了一种在液滴致动器中的液滴内分散或循环磁响应珠的方法。 在一个实施例中，本发明利用具有配置成传送液滴的多个液滴操作电极的液滴致动器和存在于多个液滴操作电极的一部分处的磁场。 在均匀磁场存在的情况下，在液滴致动器上提供含珠的液滴。 在孵育期间，通过在大气场的均匀区域内的液滴上进行液滴操作，珠子在液滴中循环。 还提供了其他实施例。

公开(公告)号：US20140231259A1

公开(公告)日：2014-08-21

申请号：US14253556

申请日：2014-04-15

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vijay Srinivasan ,  Michael G. Pollack ,  Philip Y. Paik ,  Vamsee K. Pamula

IPC: G01N27/447

CPC classification number: B01L3/502792 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502784 ,  B01L7/52 ,  B01L7/525 ,  B01L7/54 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0427 ,  B01L2400/0448 ,  B03C5/02 ,  B81B1/00 ,  G01N27/447 ,  G01N2035/1046

Abstract: The present invention relates to filler fluids for droplet operations. According to one embodiment of this aspect, a droplet microactuator including an opaque filler fluid and a transparent droplet thereon is provided. The droplet microactuator may further include a first substrate comprising electrodes configured to effect electrowetting mediated droplet operations on a surface of the first substrate; a second substrate spaced apart from the surface of the first substrate by a distance sufficient to define an interior volume between the first substrate and second substrate; and wherein the droplet and the filler fluid are in the interior volume, and wherein the droplet is surrounded by the filler fluid and arranged with respect to the electrodes in a manner which permits electrowetting mediated droplet operations to be effected on the droplet using the electrodes.

Abstract translation: 本发明涉及用于液滴操作的填充流体。 根据该方面的一个实施例，提供了包括不透明填充流体和其上的透明液滴的液滴微致动器。 液滴微致动器还可以包括第一衬底，其包括被配置为在第一衬底的表面上实现电润湿介导的液滴操作的电极; 第二基板，与第一基板的表面间隔开足以限定第一基板和第二基板之间的内部空间的距离; 并且其中所述液滴和所述填充流体在所述内部容积中，并且其中所述液滴被所述填充流体包围并且以允许使用所述电极对所述液滴进行电润湿介导的液滴操作的方式相对于所述电极布置。

公开(公告)号：US10139403B2

公开(公告)日：2018-11-27

申请号：US15266693

申请日：2016-09-15

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vamsee K. Pamula ,  Ramakrishna Sista ,  Vijay Srinivasan ,  Michael G. Pollack ,  Allen Eckhardt

IPC: B01L3/00 ,  G01N1/00 ,  G01N1/10 ,  G01N15/06 ,  G01N1/18 ,  G01N33/543 ,  B01F11/00 ,  B01F13/00 ,  G01N1/38 ,  B01F13/08 ,  B01L7/00 ,  G01N35/10

Abstract: The invention provides a method of circulating magnetically responsive beads within a droplet in a droplet actuator. The invention also provides methods for splitting droplets. The invention, in one embodiment, makes use of a droplet actuator with top and bottom substrates, a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable, and a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces. A droplet is positioned between the top and bottom surfaces and at least one of the magnetic fields is selectively altered.

公开(公告)号：US20160318021A1

公开(公告)日：2016-11-03

申请号：US15206860

申请日：2016-07-11

Applicant: Advanced Liquid Logic Inc.

Inventor： Vamsee K. Pamula ,  Michael G. Pollack ,  Vijay Srinivasan ,  Philip Paik

IPC: B01L3/00

CPC classification number: B01L3/502792 ,  B01L3/0241 ,  B01L3/50273 ,  B01L2200/06 ,  B01L2300/06 ,  B01L2400/0427 ,  B41J2/14

Abstract: A method of manipulating a droplet comprising providing a substrate comprising a surface; an elongated transport electrode disposed on the substrate surface, the elongated transport electrode having a first and a second end and configured to impart a gradient force to the droplet; and one or more wires for providing power to the transport electrode; and providing power to the one or more wires to effect the gradient force and thereby transport the droplet along the length of the elongated transport electrode from the first end to the second end.