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114 条记录 (耗时 0.048 秒)

    Droplet Actuator Loading and Target Concentration
    51.
    发明申请
    Droplet Actuator Loading and Target Concentration 审中-公开
    液滴致动器装载和目标浓度

    公开(公告)号：US20130146461A1

    公开(公告)日：2013-06-13

    申请号：US13686495

    申请日：2012-11-27

    申请人： Vamsee K. Pamula ,  Michael G. Pollack ,  Vijay Srinivasan

    发明人： Vamsee K. Pamula ,  Michael G. Pollack ,  Vijay Srinivasan

    IPC分类号： C25B15/00

    CPC分类号： C25B15/00 ,  B01F13/0071 ,  B01L3/502761 ,  B01L3/502792 ,  B01L2200/0605 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2400/0427 ,  B01L2400/0487 ,  G01N33/5438

    摘要： A droplet actuator and method of providing a droplet comprising an target substance on the droplet actuator, and including discrete flow and continuous flow functionality. Discrete flow function is controlled by electrodes arranged for conducting droplet operations on a substrate surface. The continuous flow function includes a fluid path arranged for flowing a fluid therethrough. The discrete flow and continuous flow functions may be by a barrier, including a second fluid path through the barrier. The continuous flow function may include a capture surface comprising a component having an affinity for the target substance. Methods of the invention may include flowing a fluid comprising the target substance through the fluid path; capturing the target substance on the capture surface; and forming a droplet in the discrete flow region via the second fluid path and the electrodes comprising the captured target substance.

    摘要翻译： 液滴致动器和在液滴致动器上提供包含目标物质的液滴的方法，并且包括离散流动和连续流动功能。 离散流动功能由布置用于在基板表面上进行液滴操作的电极控制。 连续流动功能包括设置成使流体流过其中的流体路径。 离散的流动和连续流动功能可以是通过屏障，包括通过屏障的第二流体路径。 连续流动功能可以包括包含对目标物质具有亲和性的成分的捕获表面。 本发明的方法可以包括使包含目标物质的流体流过流体路径; 捕获表面上的目标物质; 以及经由所述第二流体路径在所述离散流动区域中形成液滴，并且所述电极包括所捕获的目标物质。

    Droplet actuator loading and target concentration
    53.
    发明授权
    Droplet actuator loading and target concentration 有权
    液滴致动器负载和目标浓度

    公开(公告)号：US08317990B2

    公开(公告)日：2012-11-27

    申请号：US12531835

    申请日：2008-03-24

    申请人： Vamsee K. Pamula ,  Michael G. Pollack ,  Vijay Srinivasan

    发明人： Vamsee K. Pamula ,  Michael G. Pollack ,  Vijay Srinivasan

    IPC分类号： G01N35/08 ,  G01N33/543

    CPC分类号： C25B15/00 ,  B01F13/0071 ,  B01L3/502761 ,  B01L3/502792 ,  B01L2200/0605 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2400/0427 ,  B01L2400/0487 ,  G01N33/5438

    摘要： A droplet actuator and method of providing a droplet comprising an target substance on the droplet actuator, and including discrete flow and continuous flow functionality. Discrete flow function is controlled by electrodes arranged for conducting droplet operations on a substrate surface. The continuous flow function includes a fluid path arranged for flowing a fluid therethrough. The discrete flow and continuous flow functions may be by a barrier, including a second fluid path through the barrier. The continuous flow function may include a capture surface comprising a component having an affinity for the target substance. Methods of the invention may include flowing a fluid comprising the target substance through the fluid path; capturing the target substance on the capture surface; and forming a droplet in the discrete flow region via the second fluid path and the electrodes comprising the captured target substance.

    摘要翻译： 液滴致动器和在液滴致动器上提供包含目标物质的液滴的方法，并且包括离散流动和连续流动功能。 离散流动功能由布置用于在基板表面上进行液滴操作的电极控制。 连续流动功能包括设置成使流体流过其中的流体路径。 离散的流动和连续流动功能可以是通过屏障，包括通过屏障的第二流体路径。 连续流动功能可以包括包含对目标物质具有亲和性的成分的捕获表面。 本发明的方法可以包括使包含目标物质的流体流过流体路径; 捕获表面上的目标物质; 以及经由所述第二流体路径在所述离散流动区域中形成液滴，并且所述电极包括所捕获的目标物质。

    Capacitance Detection in a Droplet Actuator
    56.
    发明申请
    Capacitance Detection in a Droplet Actuator 有权
    滴液致动器中的电容检测

    公开(公告)号：US20100194408A1

    公开(公告)日：2010-08-05

    申请号：US12527208

    申请日：2008-02-15

    申请人： Ryan A. Sturmer ,  Michael G. Pollack ,  Vamsee K. Pamula ,  Vijay Srinivasan ,  Philip Y. Paik

    发明人： Ryan A. Sturmer ,  Michael G. Pollack ,  Vamsee K. Pamula ,  Vijay Srinivasan ,  Philip Y. Paik

    IPC分类号： G01R27/26

    CPC分类号： B01L3/50273 ,  B01L3/502715 ,  B01L3/502784 ,  B01L2200/0605 ,  B01L2300/0627 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0809 ,  B01L2400/0427 ,  G01N27/22 ,  G01N27/223 ,  G01N27/227 ,  G01N2203/00 ,  G01V3/02

    摘要： A method, circuit and apparatus for detecting capacitance on a droplet actuator, inter alia, for determining the presence, partial presence or absence of a droplet at an electrode on a droplet actuator by: (a) providing a droplet actuator comprising: (i) a substrate comprising electrodes arranged on the substrate for conducting droplet operations on a surface of the substrate; (ii) a capacitance detection circuit for detecting capacitance at the droplet operations surface at one or more of the electrodes; (b) detecting capacitance at the droplet operations surface at one or more of the electrodes; and (c) determining from the capacitance the presence, partial presence or absence of a droplet at the droplet operations surface at the electrode.

    摘要翻译： 一种用于检测液滴致动器上电容的方法，电路和装置，特别是用于通过以下步骤确定液滴致动器上的电极处的液滴的存在，部分存在或不存在：（a）提供液滴致动器，其包括：（i） 基板，其包括布置在所述基板上的电极，用于在所述基板的表面上进行液滴操作; （ii）电容检测电路，用于检测一个或多个电极处的液滴操作表面处的电容; （b）在一个或多个电极处检测液滴操作表面处的电容; 和（c）根据电容确定在电极处的液滴操作表面处的液滴的存在，部分存在或不存在。

    Droplet Actuator Structures
    57.
    发明申请
    Droplet Actuator Structures 审中-公开
    液滴致动器结构

    公开(公告)号：US20100025250A1

    公开(公告)日：2010-02-04

    申请号：US12529041

    申请日：2008-03-03

    申请人： Vamsee K. Pamula ,  Michael G. Pollack ,  Vijay Srinivasan ,  Philip Paik

    发明人： Vamsee K. Pamula ,  Michael G. Pollack ,  Vijay Srinivasan ,  Philip Paik

    IPC分类号： B41J2/045 ,  B03C9/00

    CPC分类号： B01L3/502792 ,  B01L3/0241 ,  B01L3/50273 ,  B01L2200/06 ,  B01L2300/06 ,  B01L2400/0427 ,  B41J2/14

    摘要： The objective of this research is to model and design a microfluidic system that uses electrostatic fields to induce movement of discrete droplets of solution. Of particular interest is movement of droplets of H2O for use in biological testing with lab-on a-chip and μTAS systems. Using computer modeling, the electric-fields for planar electrode configurations positioned on an insulating substrate are calculated for a hemispherical drop of H2O on the substrate at various positions. From these electric-fields the force on the drop is calculated. These models show that electrostatic actuation of droplets of H2O is possible. However, as the complexity of the model increases the properties of the system become less desirable and actuation may not be possible. Using microfabrication techniques, the modeled microfluidic systems have been built for testing using a Kapton substrate with copper electrodes. Hexadecenyltrichlorosilane (HTS), a self-assembled monolayer, and its oxidant have been studied and found capable of providing hydrophobic and hydrophilic surface coatings for the systems.

    摘要翻译： 本研究的目的是建立和设计一个使用静电场诱导离散液滴移动的微流体系统。 特别令人感兴趣的是使用实验室上的芯片和muTAS系统用于生物测试的H 2 O滴。 使用计算机建模，计算位于绝缘基板上的平面电极配置的电场，用于在不同位置的衬底上的半球形H 2 O的下降。 从这些电场计算出液滴上的力。 这些模型显示H2O的液滴的静电驱动是可能的。 然而，随着模型的复杂性增加，系统的性能变得不太可取，并且致动可能是不可能的。 使用微细加工技术，已经建立了用于使用具有铜电极的Kapton衬底进行测试的建模的微流体系统。 已经研究了十六碳烯三氯硅烷（HTS），一种自组装单层及其氧化剂，能够为系统提供疏水和亲水的表面涂层。

    Droplet actuator devices and methods for immunoassays and washing
    60.
    发明授权
    Droplet actuator devices and methods for immunoassays and washing 有权
    滴液致动器装置和免疫测定和洗涤方法

    公开(公告)号：US08685754B2

    公开(公告)日：2014-04-01

    申请号：US12393534

    申请日：2009-02-26

    申请人： Michael G. Pollack ,  Vamsee K. Pamula ,  Vijay Srinivasan ,  Richard B. Fair

    发明人： Michael G. Pollack ,  Vamsee K. Pamula ,  Vijay Srinivasan ,  Richard B. Fair

    IPC分类号： G01N33/553

    CPC分类号： G01N33/54333

    摘要： Droplet actuator devices and methods for immunoassays and washing are provided. According to one embodiment, a method of providing a droplet in contact with a surface of a super paramagnetic bead with a reduced concentration of a substance is provided and includes: (a) providing a super paramagnetic bead in contact with a droplet comprising a starting concentration and starting quantity of the substance and having a starting volume; (b) conducting one or more droplet operations to merge a wash droplet with the droplet provided in step (a) to yield a combined droplet; and (c) conducting one or more droplet operations to divide the combined droplet to yield a set of droplets. The set of droplets includes: (i) a droplet in contact with the super paramagnetic bead having a decreased concentration of the substance relative to the starting concentration; and (ii) a droplet which is separated from the super paramagnetic bead.

    摘要翻译： 提供液滴致动器装置和免疫测定和洗涤方法。 根据一个实施方案，提供了一种提供与物质浓度降低的超顺磁珠的表面接触的液滴的方法，包括：（a）提供与包含起始浓度的液滴接触的超顺磁珠 并开始物质的起始体积; （b）进行一个或多个液滴操作以将洗涤液滴与步骤（a）中提供的液滴合并以产生组合液滴; 和（c）进行一个或多个液滴操作以分开组合液滴以产生一组液滴。 一组液滴包括：（i）与超顺磁珠接触的液滴，其物质相对于起始浓度降低; 和（ii）从超顺磁珠分离的液滴。