公开(公告)号：US07322254B2

公开(公告)日：2008-01-29

申请号：US10734717

申请日：2003-12-12

Applicant: William Bedingham ,  Barry W. Robole

Inventor： William Bedingham ,  Barry W. Robole

IPC: G01N1/00

CPC classification number: G01N21/05 ,  B01J2219/00891 ,  B01L3/502738 ,  B01L2300/0806 ,  B01L2300/0864 ,  B01L2400/0409 ,  B01L2400/0677

Abstract: Sample processing devices with variable valve structures and methods of using the same are disclosed. The valve structures allow for removal of selected portions of the sample material located within the process chamber. Removal of the selected portions is achieved by forming an opening in a valve septum at a desired location. The valve septums may be large enough to allow for adjustment of the location of the opening based on the characteristics of the sample material in the process chamber. If the sample processing device is rotated after the opening is formed, the selected portion of the material located closer to the axis of rotation exits the process chamber through the opening. The remainder of the sample material cannot exit through the opening because it is located farther from the axis of rotation than the opening.

Abstract translation: 公开了具有可变阀结构的样品处理装置及其使用方法。 阀结构允许去除位于处理室内的样品材料的选定部分。 通过在期望的位置处在阀隔膜中形成开口来实现所选部分的移除。 阀隔件可以足够大以允许基于处理室中的样品材料的特性来调节开口的位置。 如果样品处理装置在形成开口后旋转，则位于更靠近旋转轴线的材料的选定部分通过开口离开处理室。 样品材料的其余部分不能通过开口离开，因为它比开口更远离旋转轴线。

公开(公告)号：US20070010007A1

公开(公告)日：2007-01-11

申请号：US11174757

申请日：2005-07-05

Applicant: James Aysta ,  William Bedingham ,  Barry Robole

Inventor： James Aysta ,  William Bedingham ,  Barry Robole

IPC: C12M1/34

CPC classification number: B01L9/52 ,  B01L3/50851 ,  B01L3/50853 ,  B01L7/52 ,  B01L2200/0689 ,  B01L2300/041 ,  B01L2300/0803 ,  B01L2300/1805 ,  B01L2300/1861 ,  G01N35/025 ,  G01N2035/00366 ,  Y10S435/809

Abstract: Sample processing systems and methods of using those systems for processing sample materials located in sample processing devices are disclosed. The sample processing systems include a rotating base plate on which the sample processing devices are located during operation of the systems. The systems also include a cover and compression structure designed to force a sample processing device towards the base plate. The preferred result is that the sample processing device is forced into contact with a thermal structure on the base plate. The systems and methods of the present invention may include one or more of the following features to enhance thermal coupling between the thermal structure and the sample processing device: a shaped transfer surface, magnetic compression structure, and floating or resiliently mounted thermal structure. The methods may preferably involve deformation of a portion of a sample processing device to conform to a shaped transfer surface.

Abstract translation: 公开了使用这些系统来处理位于样品处理装置中的样品材料的样品处理系统和方法。 样品处理系统包括在系统操作期间样品处理装置位于其上的旋转底板。 该系统还包括设计成迫使样品处理装置朝向底板的盖和压缩结构。 优选的结果是，样品处理装置被迫与基板上的热结构接触。 本发明的系统和方法可以包括以下特征中的一个或多个，以增强热结构和样品处理装置之间的热耦合：成形转印表面，磁性压缩结构以及浮动或弹性安装的热结构。 该方法可以优选地包括样品处理装置的一部分的变形以符合成形的转印表面。

公开(公告)号：US20050031494A1

公开(公告)日：2005-02-10

申请号：US10929044

申请日：2004-08-27

Applicant: Michael Harms ,  William Bedingham ,  Christopher Kokaisel ,  Barry Robole ,  Kenneth Wood

Inventor： Michael Harms ,  William Bedingham ,  Christopher Kokaisel ,  Barry Robole ,  Kenneth Wood

IPC: G01N1/00 ,  B01J19/00 ,  B01L3/00 ,  B01L7/00 ,  B01L9/00 ,  B81B1/00 ,  C12M1/00 ,  F27B9/16 ,  F27D5/00 ,  G01N1/28 ,  G01N35/00 ,  G01N37/00

CPC classification number: G01N35/00069 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/502707 ,  B01L3/502738 ,  B01L7/52 ,  B01L7/525 ,  B01L9/527 ,  B01L2200/0642 ,  B01L2200/0689 ,  B01L2200/142 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2400/0406 ,  B01L2400/0409 ,  B01L2400/0481 ,  B01L2400/0655 ,  B01L2400/0661 ,  F27B9/16 ,  F27D5/00 ,  Y10T436/25 ,  Y10T436/2575

Abstract: Methods and devices for thermal processing of multiple samples at the same time are disclosed. The assemblies include carriers and sample processing devices with process arrays that include conduits useful in distributing sample materials to a group pf process chambers located in fluid communication with the main conduits. The sample processing devices may include one or more of the following features in various combinations: deformable seals, process chambers connected to the main conduit by feeder conduits exiting the main conduit at offset locations, U-shaped loading chambers, and a combination of melt bonded and adhesively bonded areas. The carriers may be used to apply selective compression to the sample processing devices.

Abstract translation: 同时公开了多个样品的热处理方法和装置。 组件包括具有过程阵列的载体和样品处理装置，其包括可用于将样品材料分配到与主管道流体连通的组pf处理室的导管。 样品处理装置可以包括以下各种组合中的一个或多个以下特征：可变形密封件，通过在偏移位置处离开主管道的进料管道连接到主管道的处理室，U形加载室和熔融粘合的组合 和粘合区域。 载体可以用于对样品处理装置施加选择性压缩。

公开(公告)号：US06662830B2

公开(公告)日：2003-12-16

申请号：US10347667

申请日：2003-01-21

Applicant: William Bedingham ,  Raj Rajagopal ,  Kannan Seshadri

Inventor： William Bedingham ,  Raj Rajagopal ,  Kannan Seshadri

IPC: G01N2700

CPC classification number: G01N27/44704

Abstract: Sample processing devices with electrophoresis channels and methods of loading the electrophoresis channels with electrophoresis sieving polymer while rotating the sample processing device are disclosed. In some instances, the electrophoresis channels may be arranged radially relative to the axis of rotation of the sample processing device. In other sample processing devices, the electrophoresis channels may be arranged in curved arcs that are concentric about the center of the sample processing device (which preferably corresponds to the axis of rotation).

Abstract translation: 公开了具有电泳通道的样品处理装置和在旋转样品处理装置的同时将电泳通道与电泳筛分聚合物一起加载的方法。 在一些情况下，电泳通道可以相对于样品处理装置的旋转轴线径向布置。 在其它样品处理装置中，电泳通道可以布置在与样品处理装置的中心（其优选地对应于旋转轴线）同心的弧形弧中。

公开(公告)号：US5335658A

公开(公告)日：1994-08-09

申请号：US906740

申请日：1992-06-29

Applicant: William Bedingham

Inventor： William Bedingham

IPC: A61B5/145 ,  A61B5/00 ,  A61B5/1459 ,  A61B5/155 ,  A61B5/14

CPC classification number: A61B5/6852 ,  A61B5/1459 ,  A61B5/15003 ,  A61B5/150221 ,  A61B5/150992 ,  A61B5/155

Abstract: A parameter of blood is sensed in vivo with a system which includes a catheter and a probe. The catheter has a lumen extending therethrough, a proximal end, a distal end and a distal opening at the distal end. The probe includes one or more sensing elements at its distal end. In one embodiment, the sensing element or elements are located at least about 4 mm proximal of the distal opening of the lumen. A volume oscillator is provided to control the movement of blood into and out of the lumen. A flush solution is introduced into the lumen so that when the volume oscillator is inactive the sensing element or elements are exposed to pure flush solution, which preferably includes an anti-clotting component. The probe is received within the catheter and affixed thereto. The volume oscillator is activated to move blood into and out of the lumen to expose the sensing element or elements to blood so that they can sense the blood parameter or parameters of interest.

Abstract translation: 使用包括导管和探针的系统在体内检测血液的参数。 导管具有延伸穿过其中的内腔，近端，远端和远端处的远端开口。 探针在其远端包括一个或多个感测元件。 在一个实施例中，感测元件位于管腔的远端开口的至少约4毫米处。 提供体积振荡器以控制血液进出管腔的运动。 将冲洗溶液引入内腔，使得当体积振荡器不活动时，感测元件暴露于纯粹的冲洗溶液，其优选包括抗凝血组分。 探针被接纳在导管内并固定在其上。 体积振荡器被激活以将血液移入和流出管腔以将感测元件暴露于血液，使得它们可以感测血液参数或感兴趣的参数。

公开(公告)号：US5333609A

公开(公告)日：1994-08-02

申请号：US885713

申请日：1992-05-19

Applicant: William Bedingham ,  Joel R. Dufresne ,  David F. Wirt

Inventor： William Bedingham ,  Joel R. Dufresne ,  David F. Wirt

IPC: A61B5/145 ,  A61B5/00 ,  A61B5/03 ,  A61B5/1459 ,  A61M25/00

CPC classification number: A61B5/1459 ,  A61B5/032

Abstract: An assembly for insertion into the cardiovascular system of a patient comprising a catheter having first and second lumens and a distal port. The first lumen opens at the distal port. A probe is received in the second lumen. The probe includes a sensor responsive to a constituent of blood for providing a signal which is related to the constituent. The catheter has a window for allowing the constituent of blood to pass from outside the catheter to the sensor while substantially preventing other constituents of blood from passing from the outside of the catheter to the sensor.

Abstract translation: 一种用于插入患者的心血管系统的组件，包括具有第一和第二腔和远端口的导管。 第一个管腔在远端口开口。 在第二管腔中接收探针。 探针包括响应于血液成分的传感器，用于提供与成分相关的信号。 导管具有用于允许血液成分从导管外部传递到传感器的窗口，同时基本上防止血液的其它成分从导管的外部通过到传感器。

公开(公告)号：US08834792B2

公开(公告)日：2014-09-16

申请号：US12617921

申请日：2009-11-13

Applicant: Barry W. Robole ,  William Bedingham ,  Peter D. Ludowise ,  Jeffrey C. Pederson

Inventor： Barry W. Robole ,  William Bedingham ,  Peter D. Ludowise ,  Jeffrey C. Pederson

IPC: G01N9/30 ,  G01N21/00 ,  B01L3/00 ,  B01L7/00

CPC classification number: B01L3/5027 ,  B01L7/52 ,  B01L2300/046 ,  B01L2300/0803 ,  B01L2300/0861 ,  B01L2300/1883 ,  Y10T436/11

Abstract: A system and method for processing sample processing devices. The system can include a base plate adapted to rotate about a rotation axis. The system can further include a cover including a first projection, and a housing. A portion of the housing can be movable with respect to the base plate between an open position and a closed position, and can include a second projection. The first projection and the second projection can be adapted to be coupled together when the portion is in the open position and decoupled when the portion is in the closed position. The method can include coupling the cover to the portion of the housing, moving the portion of the housing from the open position to the closed position, and rotating the base plate about the rotation axis.

Abstract translation: 一种处理样品处理装置的系统和方法。 该系统可以包括适于围绕旋转轴线旋转的基板。 该系统还可以包括包括第一突起和壳体的盖。 壳体的一部分可以相对于基板在打开位置和关闭位置之间移动，并且可以包括第二突起。 当该部分处于打开位置时，第一突起和第二突出部可以适于联接在一起，并且当该部分处于关闭位置时该第一突出部和第二突出部分被解耦。 该方法可以包括将盖连接到壳体的部分，将壳体的部分从打开位置移动到关闭位置，并且使基板围绕旋转轴线旋转。

公开(公告)号：US20130122508A1

公开(公告)日：2013-05-16

申请号：US13699761

申请日：2011-05-23

Applicant: William Bedingham ,  James E. Aysta ,  Barry W. Robole ,  Kenneth B. Wood ,  Theresa J. Gerten ,  Christopher R. Kokaisel ,  David J. Franta ,  Kurt J. Halverson ,  Joel R. Dufresne

Inventor： William Bedingham ,  James E. Aysta ,  Barry W. Robole ,  Kenneth B. Wood ,  Theresa J. Gerten ,  Christopher R. Kokaisel ,  David J. Franta ,  Kurt J. Halverson ,  Joel R. Dufresne

IPC: G01N1/28 ,  B01L3/00

CPC classification number: G01N1/28 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502738 ,  B01L3/523 ,  B01L7/52 ,  B01L9/527 ,  B01L2200/0642 ,  B01L2200/0689 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0887 ,  G01N2035/00158 ,  Y10T436/25

Abstract: Methods and devices for the thermal processing of samples are disclosed, including sample processing devices featuring an overflow region for retaining excess fluid, as well as portable sealing apparatuses for occluding channels in a sample processing device.

Abstract translation: 公开了用于样品的热处理的方法和装置，包括具有用于保留过量流体的溢流区域的样品处理装置，以及用于封闭样品处理装置中的通道的便携式密封装置。

公开(公告)号：US08128893B2

公开(公告)日：2012-03-06

申请号：US11962703

申请日：2007-12-21

Applicant: William Bedingham ,  Christopher R. Kokaisel ,  Jeffrey C. Pederson

Inventor： William Bedingham ,  Christopher R. Kokaisel ,  Jeffrey C. Pederson

IPC: B01L7/00

CPC classification number: G01N21/11 ,  B01L3/50273 ,  B01L2300/0806 ,  B01L2300/087 ,  B01L2300/1822 ,  B01L2300/1861 ,  B01L2400/0409 ,  B01L2400/0442 ,  B01L2400/0677 ,  G01N21/07 ,  Y10T137/6579

Abstract: Processing devices that include one or more process arrays with thermal transfer structures that can be used alone or in conjunction with gravity/rotation to transport fluids within a microfluidic system. The thermal transport function can be accomplished by changing the temperature of one or more chambers to create a vacuum to draw fluids in selected directions within the process array. The methods and apparatus of the present invention may provide the ability to move fluids in a direction that is against the direction of gravity or any centrifugal forces generated by rotating a processing device using the thermal transfer structures. In other words, fluids may be moved against the direction of gravity or towards the axis of rotation using the thermally-activated vacuum.

Abstract translation: 包括具有热转移结构的一个或多个过程阵列的处理装置，其可以单独使用或与重力/旋转结合使用以在微流体系统内输送流体。 热传递功能可以通过改变一个或多个室的温度来实现，以产生真空以在流程阵列内的选定方向抽取流体。 本发明的方法和装置可以提供沿着重力方向移动流体的能力，或者通过使用热传递结构旋转处理装置产生的任何离心力。 换句话说，流体可以使用热活化的真空来抵抗重力方向或朝向旋转轴线移动。

公开(公告)号：US08092759B2

公开(公告)日：2012-01-10

申请号：US12821772

申请日：2010-06-23

Applicant: William Bedingham ,  Barry W. Robole

Inventor： William Bedingham ,  Barry W. Robole

IPC: G01N33/00

CPC classification number: B01L3/502715 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2300/044 ,  B01L2300/0803 ,  B01L2300/0887 ,  B01L2300/1805

Abstract: Microfluidic sample processing disks with a plurality of fluid structures formed therein are disclosed. Each of the fluid structures preferably includes an input well and one or more process chambers connected to the input well by one or more delivery channels. The process chambers may be arranged in a compliant annular processing ring that is adapted to conform to the shape of an underlying thermal transfer surface under pressure. That compliance may be delivered in the disks of the present invention by locating the process chambers in an annular processing ring in which a majority of the volume is occupied by the process chambers. Compliance within the annular processing ring may alternatively be provided by a composite structure within the annular processing ring that includes covers attached to a body using pressure sensitive adhesive.

Abstract translation: 公开了具有形成在其中的多个流体结构的微流体样品处理盘。 每个流体结构优选地包括输入井和通过一个或多个输送通道连接到输入井的一个或多个处理室。 处理室可以布置成柔顺的环形加工环，其适于在压力下符合下面的热转印表面的形状。 通过将处理室定位在其中大部分体积被处理室占据的环形处理环中，可以在本发明的盘中传送顺从性。 环形加工环内的合规性可替代地由环形加工环内的复合结构提供，其包括使用压敏粘合剂附接到主体的盖。