中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

89 records (took 0.047 seconds)

    ACTIVATED SPLIT-POLYPEPTIDES AND METHODS FOR THEIR PRODUCTION AND USE
    61.
    发明申请
    ACTIVATED SPLIT-POLYPEPTIDES AND METHODS FOR THEIR PRODUCTION AND USE 审中-公开
    活性分离多肽及其生产和使用方法

    公开(公告)号：US20090220942A1

    公开(公告)日：2009-09-03

    申请号：US12091709

    申请日：2006-10-27

    Applicant: Natalia Broude ,  Charles R. Cantor ,  Vadim V. Demidov

    Inventor： Natalia Broude ,  Charles R. Cantor ,  Vadim V. Demidov

    IPC: C12Q1/70 ,  C12Q1/68 ,  C07K14/00 ,  C12P21/00

    CPC classification number: C12Q1/6883

    Abstract: The present invention relates to a method to produce activated split-polypeptide fragments that on reconstitution immediately forms an active protein. The method relate to real-time protein complementation. Also encompassed in the invention is a method to split and produce split-fluorescent proteins in an active state which produce a fluorescent signal immediately on reconstitution. The present application also provides methods to detect nucleic acids; non-nucleic acid analytes and nucleic acid hybridization in real-time using the novel activated split-polypeptide fragments of the invention.

    Abstract translation: 本发明涉及一种产生活化的裂多肽片段的方法，其在重构时立即形成活性蛋白质。 该方法涉及实时蛋白质互补。 本发明还包括分裂和产生活性状态的分裂荧光蛋白的方法，其在重建时立即产生荧光信号。 本申请还提供了检测核酸的方法; 非核酸分析物和核酸杂交，使用本发明的新型活化的裂解多肽片段。

    SINGLE MOLECULE NUCLEIC ACID SEQUENCE ANALYSIS PROCESSES AND COMPOSITIONS
    62.
    发明申请
    SINGLE MOLECULE NUCLEIC ACID SEQUENCE ANALYSIS PROCESSES AND COMPOSITIONS 有权
    单分子核酸序列分析方法和组合物

    公开(公告)号：US20090202984A1

    公开(公告)日：2009-08-13

    申请号：US12354749

    申请日：2009-01-15

    Applicant: Charles R. Cantor

    Inventor： Charles R. Cantor

    IPC: C12Q1/70 ,  C12Q1/68

    CPC classification number: C12Q1/6876 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6844 ,  C12Q2565/537 ,  C12Q2565/519 ,  C12Q2565/501 ,  C12Q2531/101 ,  C12Q2525/161 ,  C12Q2525/155

    Abstract: Improved solid supports and methods for analyzing target nucleotide sequences are provided herein. Certain improvements are directed to efficiently preparing nucleic acids that comprise nucleotide sequences identical to or substantially identical to one or more target nucleotide sequences, or complement thereof. The prepared nucleic acids include a reference sequence that facilitates sequence analysis. The solid supports and methods provided herein minimize the number of steps required by published sequence analysis methodologies, and thereby offer improved sequence analysis efficiency.

    Abstract translation: 本文提供了改进的固体支持物和分析靶核苷酸序列的方法。 某些改进旨在有效地制备包含与一个或多个靶核苷酸序列或其互补序列相同或基本相同的核苷酸序列的核酸。 所制备的核酸包括促进序列分析的参考序列。 本文提供的固体支持物和方法使发布的序列分析方法所需的步骤数量最小化，从而提供改进的序列分析效率。

    Method for Detecting and Quantifying Rare Mutations/Polymorphisms
    65.
    发明申请
    Method for Detecting and Quantifying Rare Mutations/Polymorphisms 有权
    检测和定量稀有突变/多态性的方法

    公开(公告)号：US20080032287A1

    公开(公告)日：2008-02-07

    申请号：US10589709

    申请日：2005-02-18

    Applicant: Charles R. Cantor ,  Chunming Ding

    Inventor： Charles R. Cantor ,  Chunming Ding

    IPC: C12Q1/68

    CPC classification number: C12Q1/6827 ,  C12Q2600/156 ,  C12Q2525/186 ,  C12Q2535/125 ,  C12Q2565/627 ,  C12Q2545/101

    Abstract: The present invention is directed to a method for detecting and quantifying rare mutations in a nucleic acid sample. The nucleic acid molecules under investigation can be either DNA or RNA. The rare mutation can be any type of functional or non-functional nucleic acid change or mutation, such as deletion, insertion, translocation, inversion, one or more base substitution or polymorphism. Therefore, the methods of the present invention are useful in detection of rare mutations in, for example, diagnostic, prognostic and follow-up applications, when the targets are rare known nucleic acid variants mixed in with the wildtype or the more common nucleic acid variant(s).

    Abstract translation: 本发明涉及一种用于检测和定量核酸样品中罕见突变的方法。 正在研究的核酸分子可以是DNA或RNA。 罕见突变可以是任何类型的功能或非功能性核酸变化或突变，例如缺失，插入，易位，反转，一个或多个碱基取代或多态性。 因此，当目标是罕见的已知的与野生型或更常见的核酸变体混合的核酸变体时，本发明的方法可用于检测例如诊断，预后和随访应用中的罕见突变 （s）。

    Streptavidin mutants
    67.
    发明授权
    Streptavidin mutants 失效
    链霉亲和素突变体

    公开(公告)号：US6022951A

    公开(公告)日：2000-02-08

    申请号：US628540

    申请日：1996-04-10

    Applicant: Takeshi Sano ,  Charles R. Cantor ,  Sandor Vajda ,  Gabriel O. Reznik ,  Cassandra L. Smith ,  Mark W. Pandori

    Inventor： Takeshi Sano ,  Charles R. Cantor ,  Sandor Vajda ,  Gabriel O. Reznik ,  Cassandra L. Smith ,  Mark W. Pandori

    IPC: A61K38/00 ,  C07K14/36 ,  C12N1/21 ,  C12N15/31 ,  G01N33/538

    CPC classification number: G01N33/538 ,  C07K14/36 ,  A61K38/00 ,  G01N2333/465 ,  Y10S530/808 ,  Y10S530/81

    Abstract: The present invention relates to streptavidin proteins and peptides having a altered physical properties such as an increased stability or increased or decreased affinity for binding biotin. The invention also relates to methods for the detection, identification, separation and isolation of targets using streptavidin proteins or peptides. Streptavidin with increased or reduced affinity allows for the use of the streptavidin-biotin coupling systems for detection and isolation systems wherein it is necessary to remove of one or the other of the binding partners. Such systems are useful for the purification of functional proteins and viable cells. The invention also relates to nucleic acids which encode these streptavidin proteins and peptides and to recombinant cells such as bacteria, yeast and mammalian cells which contain these nucleic acids.

    Abstract translation: 本发明涉及链霉抗生物素蛋白和具有改变的物理性质例如增加的稳定性或增加或降低的结合生物素的亲和力的肽。 本发明还涉及使用链霉亲和素蛋白或肽检测，鉴定，分离和分离靶的方法。 具有增加或降低亲和力的链霉亲和素允许使用链霉抗生物素蛋白 - 生物素偶联系统进行检测和分离系统，其中必须除去一种或另一种结合配偶体。 这样的系统可用于纯化功能性蛋白质和活细胞。 本发明还涉及编码这些链霉抗生物素蛋白和肽以及含有这些核酸的重组细胞如细菌，酵母和哺乳动物细胞的核酸。

    Sequence-directed DNA binding molecules compositions and methods
    68.
    发明授权
    Sequence-directed DNA binding molecules compositions and methods 失效
    序列指导的DNA结合分子的组成和方法

    公开(公告)号：US6010849A

    公开(公告)日：2000-01-04

    申请号：US482080

    申请日：1995-06-07

    Applicant: Cynthia A. Edwards ,  Charles R. Cantor ,  Beth M. Andrews ,  Lisa M. Turin ,  Kirk E. Fry

    Inventor： Cynthia A. Edwards ,  Charles R. Cantor ,  Beth M. Andrews ,  Lisa M. Turin ,  Kirk E. Fry

    IPC: C07K14/47 ,  C12N15/10 ,  C12N15/67 ,  C12Q1/68 ,  C12Q1/70 ,  G01N33/53

    CPC classification number: C12Q1/701 ,  C07K14/47 ,  C12N15/1048 ,  C12N15/67 ,  C12Q1/6811 ,  C12Q1/6813 ,  C12Q1/6876 ,  C12Q1/6883 ,  C12Q1/705 ,  H01L2924/14

    Abstract: The present invention defines a DNA:protein-binding assay useful for screening libraries of synthetic or biological compounds for their ability to bind DNA test sequences. The assay is versatile in that any number of test sequences can be tested by placing the test sequence adjacent to a defined protein binding screening sequence. Binding of molecules to these test sequence changes the binding characteristics of the protein molecule to its cognate binding sequence. When such a molecule binds the test sequence the equilibrium of the DNA:protein complexes is disturbed, generating changes in the concentration of free DNA probe. Numerous exemplary target test sequences (SEQ ID NO:1 to SEQ ID NO:600) are set forth. The assay of the present invention is also useful to characterize the preferred binding sequences of any selected DNA-binding molecule.

    Abstract translation: 本发明定义了一种DNA：蛋白结合测定法，用于筛选合成或生物化合物文库结合DNA测试序列的能力。 该测定法是通用的，因为可以通过将测试序列置于与定义的蛋白质结合筛选序列相邻的位置来测试任何数量的测试序列。 分子与这些测试序列的结合改变了蛋白质分子与其同源结合序列的结合特征。 当这样的分子结合测试序列时，DNA：蛋白复合物的平衡受到干扰，产生游离DNA探针浓度的变化。 阐述了许多示例性目标测试序列（SEQ ID NO：1至SEQ ID NO：600）。 本发明的测定也可用于表征任何所选DNA结合分子的优选结合序列。

    Positional sequencing by hybridization
    69.
    发明授权
    Positional sequencing by hybridization 失效
    通过杂交进行位置测序

    公开(公告)号：US6007987A

    公开(公告)日：1999-12-28

    申请号：US730185

    申请日：1996-10-15

    Applicant: Charles R. Cantor ,  Marek Przetakiewicz ,  Takeshi Sano ,  Cassandra L. Smith

    Inventor： Charles R. Cantor ,  Marek Przetakiewicz ,  Takeshi Sano ,  Cassandra L. Smith

    IPC: C12Q1/68 ,  C40B40/06

    CPC classification number: C12Q1/6837 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00722 ,  C40B40/06

    Abstract: This invention is directed to methods and reagents useful for sequencing nucleic acid targets utilizing sequencing by hybridization technology comprising probes, arrays of probes and methods whereby sequence information is obtained rapidly and efficiently in discrete packages. That information can be used for the detection, identification, purification and complete or partial sequencing of a particular target nucleic acid. When coupled with a ligation step, these methods can be performed under a single set of hybridization conditions. The invention also relates to the replication of probe arrays and methods for making and replicating arrays of probes which are useful for the large scale manufacture of diagnostic aids used to screen biological samples for specific target sequences. Arrays created using PCR technology may comprise probes with 5'- and/or 3'-overhangs.

    Abstract translation: 本发明涉及可用于使用包括探针，探针阵列和方法的杂交技术测​​序的用于测序核酸靶的方法和试剂，从而在离散包装中快速有效地获得序列信息。 该信息可以用于特定靶核酸的检测，鉴定，纯化和完全或部分测序。 当与连接步骤相结合时，这些方法可以在单一杂交条件下进行。 本发明还涉及探针阵列的复制和用于制备和复制探针阵列的方法，所述探针阵列可用于大规模制造用于筛选特定靶序列的生物样品的诊断辅助物。 使用PCR技术产生的阵列可以包含具有5'和/或3'突出端的探针。

Patent Agency Ranking