摘要:
The invention provides a method for purifying viral vectors containing therapeutic genes for use in gene therapy. The invention comprises a method of purification from a cell lysate of a recombinant viral vector containing a therapeutic gene, which comprises: a) treating said lysate with an enzymatic agent that selectively degrades both unencapsulated DNA and RNA; b) chromatographing the treated lysate from step a) on a first resin; and c) chromatographing the eluant from step b) on a second resin; wherein one resin is an anion exchange resin and the other is an immobilized metal ion chromatography (IMAC) resin.
摘要:
An initial processing of natural raw rubber through an initial processing machine, comprising the steps of: (a) providing a coagulated latex which contains water and volatile compositions; (b) dewatering the coagulated latex through a screw-pressing process to remove free water; (c) forming a first pretreated latex material; (d) aging the first pretreated latex material through an aging process to remove water and volatile compositions; and (e) forming a final product of aged latex material. The screw-pressing process makes use of the temperature and pressure increase along the elongated channel structure. The aging process makes use of the further temperature and pressure increase of the rubber materials, together with the screwing effect of the screw-shaft component, the squeezing effect of the nozzle and additional heating at a particular location, which is around the mouthpiece of the nozzle to complete the aging process, which is energy saving, effective and efficient.
摘要:
This invention provides mixed-mode stationary phase compositions, devices and systems comprising the stationary phases as well as methods of producing these compositions using epoxide ring-opening reactions. Also provided are methods of using the stationary phases of the invention in separations.
摘要:
This disclosure relates to sulfur sensors that utilize sensing materials that can be used to detect a wide range of concentrations including ultra low concentrations of sulfur in liquids, such as below even 15 ppm. The sulfur sensors comprise a sensing electrode having a material that contributes an electronic output to the analysis and a material that contributes an ionic output to the analysis.
摘要:
A start-up method for a hybrid powertrain in which hydraulic pressure is generated in a casual start-up by an electric oil pump motor where certain predetermined conditions are satisfied and the casual start-up is abandoned and hydraulic pressure is generated by an engine where the predetermined conditions are not satisfied. The casual start-up is also abandoned where a vehicle operator seeks propulsion shortly after start-up and before an oil pump is rotating within a predetermined threshold of an oil pump target RPM.
摘要:
An initial processing of natural raw rubber through an initial processing machine, comprising the steps of: (a) providing a coagulated latex which contains water and volatile compositions; (b) dewatering the coagulated latex through a screw-pressing process to remove free water; (c) forming a first pretreated latex material; (d) aging the first pretreated latex material through an aging process to remove water and volatile compositions; and (e) forming a final product of aged latex material. The screw-pressing process makes use of the temperature and pressure increase along the elongated channel structure. The aging process makes use of the further temperature and pressure increase of the rubber materials, together with the screwing effect of the screw-shaft component, the squeezing effect of the nozzle and additional heating at a particular location, which is around the mouthpiece of the nozzle to complete the aging process, which is energy saving, effective and efficient.
摘要:
There are provided ligand compositions and stationary phases comprising a polyhedral oligomeric silsequioxane moiety. Also provided are chromatographic devices comprising the stationary phases, and methods of making and using the ligands, stationary phases and chromatographic devices of the invention.
摘要:
The current invention provides compositions, which are useful as stationary phases for a variety of chromatographic applications, such as high performance liquid chromatography (HPLC) and solid-phase extraction (SPE). The compositions provide both strong aromatic and hydrophobic interactions with components of a sample. Moreover, the invention provides compositions of new silanes, their immobilization on a solid substrate, such as silica, to form new stationary phases.
摘要:
The present invention provides a method of source number estimation based on eigenspace in DOA estimation, including: (1) obtaining signals received by uniform linear array; (2) calculating a covariance matrix according to the received signals; (3) calculating a complex-valued covariance matrix or a covariance matrix after real-valued computations; (4) conducting eigendecomposition to the covariance matrix to obtain its eigenvector matrix; (5) calculating a source number decision based on the eigenvector; (6) calculating a ratio; and (7) source number estimation. The present invention can accurately estimate source number and save huge amounts of computation during signal processing in DOA estimation, and lower the cost of hardware.
摘要:
In one embodiment, the present invention is directed to a pulsed electrochemical detection method comprising (a) flowing a liquid sample stream including at least one analyte past a working electrode in a flow-through cell; (b) applying at least three pulsed electrical potentials to the working electrode, (1) a first conditioning potential, (2) a second detection and oxidative cleaning potential, and (3) a third reductive cleaning potential, said second potential being higher than said first and third potentials and said first potential being higher than said third potential; and (c) detecting the output from said working electrode during the time period of applying said second potential.