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    Fluidics device
    71.
    发明授权
    Fluidics device 有权
    流体装置

    公开(公告)号：US08491791B2

    公开(公告)日：2013-07-23

    申请号：US13552356

    申请日：2012-07-18

    Applicant: Lee O. Lomas ,  Jian Ding ,  Egisto Boschetti

    Inventor： Lee O. Lomas ,  Jian Ding ,  Egisto Boschetti

    IPC: B01D63/00 ,  B01D61/00 ,  B01D15/08 ,  G01N1/00

    CPC classification number: G01N30/461 ,  B01D15/325 ,  B01D15/362 ,  B01D15/363 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/563 ,  B01L2200/026 ,  B01L2200/028 ,  B01L2300/0681 ,  B01L2300/0829 ,  B01L2300/087 ,  B01L2400/0487 ,  C07K1/34 ,  G01N27/44704 ,  G01N27/44795 ,  G01N30/466 ,  G01N30/468 ,  Y10T137/2224 ,  Y10T137/794 ,  Y10T137/86187 ,  Y10T436/255

    Abstract: The present invention contemplates various devices that are configured to separate a sample, which contains more than one unique species, into any desired number of sub-samples by passing the sample across a like number of separation media configured for a first separation protocol. Each of the sub-samples may be further separated by an additional separation protocol, thereby creating a plurality of mini-samples, each of which may be further separated and/or analyzed. The invention also contemplates using a simple method of using conduits to form a fluid path that passes through a plurality of separation media, each of which media is configured to isolate a particular sub-sample. After various sub-samples of the sample are isolated by the various separation media, the conduits may be removed, thereby enabling each of the isolated sub-samples to be further separated and/or analyzed independent of any other sub-sample.

    Abstract translation: 本发明考虑了通过将样品穿过配置用于第一分离方案的相似数量的分离介质，将被配置成将含有多于一种独特物种的样品分离成任何所需数量的子样品的各种装置。 每个子样品可以通过另外的分离方案进一步分离，从而产生多个微型样品，每个小样品可进一步分离和/或分析。 本发明还考虑使用简单的方法来使用导管来形成通过多个分离介质的流体路径，其中每个介质被配置为隔离特定的子样品。 在通过各种分离介质分离样品的各种亚样品之后，可以去除导管，从而使每个分离的子样品能够独立于任何其它亚样品进一步分离和/或分析。

    Fluidics device
    72.
    发明授权
    Fluidics device 有权
    流体装置

    公开(公告)号：US08246832B2

    公开(公告)日：2012-08-21

    申请号：US11915151

    申请日：2006-01-19

    Applicant: Lee O. Lomas ,  Jian Ding ,  Egisto Boschetti

    Inventor： Lee O. Lomas ,  Jian Ding ,  Egisto Boschetti

    IPC: B01D63/00

    CPC classification number: G01N30/461 ,  B01D15/325 ,  B01D15/362 ,  B01D15/363 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/563 ,  B01L2200/026 ,  B01L2200/028 ,  B01L2300/0681 ,  B01L2300/0829 ,  B01L2300/087 ,  B01L2400/0487 ,  C07K1/34 ,  G01N27/44704 ,  G01N27/44795 ,  G01N30/466 ,  G01N30/468 ,  Y10T137/2224 ,  Y10T137/794 ,  Y10T137/86187 ,  Y10T436/255

    Abstract: The present invention contemplates various devices that are configured to separate a sample, which contains more than one unique species, into any desired number of sub-samples by passing the sample across a like number of separation media configured for a first separation protocol. Each of the sub-samples may be further separated by an additional separation protocol, thereby creating a plurality of mini-samples, each of which may be further separated and/or analyzed. The invention also contemplates using a simple method of using conduits to form a fluid path that passes through a plurality of separation media, each of which media is configured to isolate a particular sub-sample. After various sub-samples of the sample are isolated by the various separation media, the conduits may be removed, thereby enabling each of the isolated sub-samples to be further separated and/or analyzed independent of any other sub-sample.

    Abstract translation: 本发明考虑了通过将样品穿过配置用于第一分离方案的相似数量的分离介质，将被配置成将含有多于一种独特物种的样品分离成任何所需数量的子样品的各种装置。 每个子样品可以通过另外的分离方案进一步分离，从而产生多个微型样品，每个小样品可进一步分离和/或分析。 本发明还考虑使用简单的方法来使用导管来形成通过多个分离介质的流体路径，其中每个介质被配置为隔离特定的子样品。 在通过各种分离介质分离样品的各种亚样品之后，可以去除导管，从而使每个分离的子样品能够独立于任何其它亚样品进一步分离和/或分析。

    Method for purifying proteins
    75.
    发明授权
    Method for purifying proteins 有权
    蛋白质纯化方法

    公开(公告)号：US07754861B2

    公开(公告)日：2010-07-13

    申请号：US11388181

    申请日：2006-03-22

    Applicant: Egisto Boschetti ,  Lee O. Lomas

    Inventor： Egisto Boschetti ,  Lee O. Lomas

    IPC: A23J1/00 ,  C07K14/00 ,  C07K17/00 ,  C40B40/10

    CPC classification number: C07K1/047 ,  C07K1/22

    Abstract: The present invention provides methods and kits for purifying a target protein group. The method comprises the steps of contacting a sample comprising at least 95% of the target protein group and at most 5% of contaminating proteins with a library of binding moieties having different binding moieties, binding the contaminating proteins and a minority of the target protein group to the library of binding moieties, separating the unbound target protein group from the proteins bound to the library of binding moieties and collecting the unbound target protein. The collected target protein is more pure than the target protein group in the sample.

    Abstract translation: 本发明提供了用于纯化靶蛋白质组的方法和试剂盒。 该方法包括以下步骤：将包含至少95％的目标蛋白质组和至多5％的污染蛋白质的样品与具有不同结合部分的结合部分的文库结合，结合污染蛋白质和少数靶蛋白质组 到结合部分的文库，将未结合的靶蛋白基团与结合到结合部分文库的蛋白质分离并收集未结合的靶蛋白质。 收集的目标蛋白质比样品中的目标蛋白质组更为纯净。

    PROCESS DESIGN USING MASS SPECTROMETRY
    76.
    发明申请
    PROCESS DESIGN USING MASS SPECTROMETRY 审中-公开
    使用大量光谱的工艺设计

    公开(公告)号：US20100173791A1

    公开(公告)日：2010-07-08

    申请号：US12651942

    申请日：2010-01-04

    Applicant: Egisto Boschetti

    Inventor： Egisto Boschetti

    IPC: C40B30/02 ,  C40B50/02 ,  C40B40/00

    CPC classification number: G01N33/6848 ,  C07K1/16 ,  G01N30/8675 ,  G01N2030/8831

    Abstract: The present invention provides a facile and efficient method for determining a chromatographic protocol for separating a target protein from one or more second protein impurity. Also provided is a database facilitating the determination of an appropriate separation protocol.

    Abstract translation: 本发明提供了一种用于确定用于从一种或多种第二种蛋白质杂质分离靶蛋白质的色谱方案的简便有效的方法。 还提供了便于确定适当分离方案的数据库。

    Photocrosslinked hydrogel surface coatings
    79.
    发明申请
    Photocrosslinked hydrogel surface coatings 审中-公开
    光交联水凝胶表面涂层

    公开(公告)号：US20070082019A1

    公开(公告)日：2007-04-12

    申请号：US10546173

    申请日：2004-02-20

    Applicant: Wenxi Huang ,  Yury Agroskin ,  Egisto Boschetti

    Inventor： Wenxi Huang ,  Yury Agroskin ,  Egisto Boschetti

    IPC: A61F2/02 ,  C12M1/34

    CPC classification number: H01J49/0418 ,  A61L27/52

    Abstract: A hydrogel layer is applied to a substrate advantageously when the layer is formed in situ, using a polymeric or copolymeric precursor that includes, respectively, monomer subunits that have a photocrosslinkable functionality and monomer subunits that have a chemically selective functionality for binding a biomolecular analyte, such as a protein. A hydrogel-coated substrate thus obtained is particularly useful as a probe for mass spectroscopic analysis, including SELDI analysis. Hydrogel particles also can be used for SELDI analysis.

    Abstract translation: 使用聚合物或共聚物前体分别形成具有光可交联官能团的单体亚单位和具有化学选择性功能性用于结合生物分子分析物的单体亚单位，当层原位形成时，水凝胶层有利地施加到基底上， 如蛋白质。 由此获得的水凝胶涂覆的基底特别可用作用于质谱分析的探针，包括SELDI分析。 水凝胶颗粒也可用于SELDI分析。

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