-
公开(公告)号：US08394249B2
公开(公告)日：2013-03-12
申请号：US12494927
申请日：2009-06-30
CPC分类号： B01L3/50273 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01F13/0084 , B01L3/00 , B01L3/5027 , B01L3/502784 , B01L3/502792 , B01L2200/06 , B01L2200/0605 , B01L2200/10 , B01L2300/089 , B01L2300/161 , B01L2300/165 , B01L2400/0415 , B01L2400/0427 , B41J2002/14395 , C25B9/00 , G01N27/44791 , G02B26/005
摘要： Methods are provided for manipulating droplets. The methods include providing the droplet on a surface comprising an array of electrodes and a substantially co-planer array of reference elements, wherein the droplet is disposed on a first one of the electrodes, and the droplet at least partially overlaps a second one of the electrodes and an intervening one of the reference elements disposed between the first and second electrodes. The methods further include activating the first and second electrodes to spread at least a portion of the droplet across the second electrode and deactivating the first electrode to move the droplet from the first electrode to the second electrode.
-
公开(公告)号：US07727723B2
公开(公告)日：2010-06-01
申请号：US11639710
申请日：2006-12-15
CPC分类号： C12Q1/68 , B01L7/52 , B01L2300/0645 , B01L2300/1822 , B01L2300/1894 , B01L2400/0424 , B01L2400/0427 , C12Q1/6869 , G01N27/44791 , Y10T436/25 , C12Q2565/629 , C12Q2565/301 , C12Q2563/149
摘要： The present invention relates to a droplet microactuator and to systems, apparatuses and methods employing the droplet microactuator for executing various protocols using droplets. The invention includes a droplet microactuator or droplet microactuator system having one or more input reservoirs loaded with reagents for conducting sequencing protocols, such as the reagents for conducting a pyrosequencing protocol. The invention also includes a droplet microactuator or droplet microactuator system, having one or more input reservoirs loaded with a sample for conducting a pyrosequencing protocol.
摘要翻译： 液滴微致动器技术领域本发明涉及液滴微致动器以及使用液滴微致动器来使用液滴执行各种协议的系统，装置和方法。 本发明包括液滴微致动器或液滴微致动器系统，其具有一个或多个输入储存器，其中装载有用于进行测序方案的试剂，例如用于进行焦磷酸测序方案的试剂。 本发明还包括液滴微致动器或液滴微致动器系统，其具有装载有用于进行焦磷酸测序方案的样品的一个或多个输入储存器。
-
公开(公告)号：US08852952B2
公开(公告)日：2014-10-07
申请号：US12990815
申请日：2009-05-04
申请人： Michael G. Pollack , Prasanna Thwar , Vamsee K. Pamula , Allen Eckhardt , Alexander Shenderov , Dwayne Allen , Vijay Srinivasan
发明人： Michael G. Pollack , Prasanna Thwar , Vamsee K. Pamula , Allen Eckhardt , Alexander Shenderov , Dwayne Allen , Vijay Srinivasan
IPC分类号： G01N1/00
CPC分类号： B01L3/502784 , B01L3/502715 , B01L3/502761 , B01L7/52 , B01L2200/027 , B01L2200/0673 , B01L2300/022 , B01L2300/023 , B01L2300/024 , B01L2300/0672 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0478 , B01L2400/0481 , B01L2400/0487 , B01L2400/049 , B01L2400/0683 , G01N27/44704 , G01N35/0098 , G01N2035/1046 , Y10T436/25 , Y10T436/2575
摘要： The invention provides droplet actuators and droplet actuator cassettes including reagent storage capabilities, as well as methods of making and using the droplet actuators and cassettes. The invention also provides continuous flow channel elements and techniques for using electrodes to manipulate droplets in flowing streams. The invention also discloses methods of separating compounds on a droplet actuator. Various other aspects of the invention are also disclosed.
摘要翻译： 本发明提供了包括试剂储存能力的液滴致动器和液滴致动器盒，以及制造和使用液滴致动器和盒的方法。 本发明还提供了用于使用电极在流动流中操纵液滴的连续流动通道元件和技术。 本发明还公开了在液滴致动器上分离化合物的方法。 还公开了本发明的各种其它方面。
-
公开(公告)号：US20130293246A1
公开(公告)日：2013-11-07
申请号：US13988190
申请日：2011-11-15
CPC分类号： G01N27/221 , B01L3/502784 , B01L3/502792 , B01L2200/14 , B01L2300/0645 , B01L2300/0816 , B01L2300/089 , B01L2400/0427 , B41J2/04541 , B41J2/04555 , B41J2/0458 , G01K7/34
摘要： The embodiments described herein provide methods of measuring capacitance, detecting a droplet at a position, determining a thickness of an oil film and determining temperature in a droplet actuator. Specifically, the capacitance detection method may be used as a real-time verification tool in order to detect the absence, presence, and/or partial presence of a droplet at an electrode, analyze droplet properties, measure droplet size or volume, optimize the speed of droplet operation and detect air bubbles.
摘要翻译： 本文所述的实施例提供了测量电容，检测位置处的液滴，确定油膜的厚度和确定液滴致动器中的温度的方法。 具体地，可以将电容检测方法用作实时验证工具，以便检测电极处的液滴的不存在，存在和/或部分存在，分析液滴属性，测量液滴尺寸或体积，优化速度 的液滴操作并检测气泡。
-
公开(公告)号：US08828655B2
公开(公告)日：2014-09-09
申请号：US13862587
申请日：2013-04-15
IPC分类号： C12Q1/00
CPC分类号： G01N21/6486 , B01F13/0809 , C07H15/04 , C12Q1/34 , G01N2333/928 , G01N2800/042
摘要： A method is provided for conducting a droplet-based enzymatic assay, e.g., for diagnostic purposes. On a droplet actuator, a droplet comprising an enzyme of interest is provided along with a droplet comprising a substrate which is potentially modified in the presence of the enzyme. Droplet operations are executed to combine the enzyme and substrate droplets on the droplet actuator, thereby yielding an assay droplet on the droplet actuator. Detecting modification of the substrate by the enzyme in the assay droplet occurs on the droplet actuator. Modified substrate preparations for conducting such enzymatic assays are also provided.
摘要翻译： 提供了用于进行基于液滴的酶测定的方法，例如用于诊断目的。 在液滴致动器上，包含感兴趣的酶的液滴与包含在酶存在下潜在地被修饰的底物的液滴一起提供。 执行液滴操作以将酶和底物液滴组合在液滴致动器上，从而在液滴致动器上产生测定液滴。 在液滴致动器中检测由测定液滴中的酶对底物的修饰。 还提供了用于进行这种酶测定的修饰底物制剂。
-
公开(公告)号：US08426213B2
公开(公告)日：2013-04-23
申请号：US12529052
申请日：2008-03-05
IPC分类号： G01N33/50
CPC分类号： C12Q1/28 , C12Q1/005 , C12Q1/006 , G01N2333/904 , Y10T436/118339 , Y10T436/14 , Y10T436/141111 , Y10T436/142222 , Y10T436/143333 , Y10T436/144444 , Y10T436/145555 , Y10T436/146666 , Y10T436/147777 , Y10T436/148888 , Y10T436/206664
摘要： The present invention relates to providing improved hydrogen peroxide assays, as well as droplet actuators for conducting such assays. The droplet actuators of the invention may be used to conduct droplet-based hydrogen peroxide assays. They may also be associated with detectors for analyzing the results of the hydrogen peroxide assays of the invention. They may be provided as components of systems which control droplet operations and/or detection for conducting the hydrogen peroxide assays. Measurement by the detector may be used to quantify the presence of an analyte in a sample.
摘要翻译： 本发明涉及提供改进的过氧化氢测定，以及用于进行这种测定的液滴致动器。 本发明的液滴致动器可用于进行基于液滴的过氧化氢测定。 它们也可以与用于分析本发明的过氧化氢测定结果的检测器相关联。 它们可以作为控制液滴操作和/或检测以进行过氧化氢测定的系统的组件提供。 检测器的测量可用于量化样品中分析物的存在。
-
公开(公告)号：US08202686B2
公开(公告)日：2012-06-19
申请号：US12497677
申请日：2009-07-05
IPC分类号： C12Q1/00
CPC分类号： C12Q1/34 , B01F13/0071 , B01L3/502784 , B01L7/54 , B01L2300/0654 , B01L2300/0829 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , G01N2021/035 , G01N2333/924 , G01N2800/042
摘要： The invention relates to a microfluidic platform and methods of using the platform for conducting enzyme assays using a droplet actuator. The enzyme assays of the invention are useful for, among other things, identifying and/or characterizing disorders resulting from conditions in which enzymes are defective or are produced in inappropriate amounts. Enzyme assays of the invention may, for example, be used to detect altered activity of a particular enzyme in a sample, which may serve as an indicator of a particular disease. Altered activity may, for example, be caused by conditions which result in the increased or reduced production of a certain enzyme or its substrate and/or conditions which result in defective enzymes and/or substrates exhibiting increased or decreased effectiveness relative to corresponding normal enzymes and/or substrates.
摘要翻译： 本发明涉及一种微流体平台和使用该平台用于使用液滴致动器进行酶测定的方法。 本发明的酶测定法尤其可用于识别和/或表征由酶缺陷或以不适当量产生的病症引起的疾病。 本发明的酶测定可以例如用于检测样品中特定酶的改变的活性，其可以用作特定疾病的指标。 改变的活性可以例如由导致某些酶或其底物的产生增加或减少的条件引起，和/或导致有缺陷的酶和/或底物相对于相应的正常酶显示出增加或降低的有效性的条件，和 /或底物。
-
公开(公告)号：US20130264010A1
公开(公告)日：2013-10-10
申请号：US13618322
申请日：2012-09-14
CPC分类号： H05K3/4038 , B01L3/502707 , B01L3/502784 , B01L3/502792 , B01L2300/089 , B01L2300/12 , B01L2400/0427 , H05K1/113 , H05K3/0058 , H05K3/0067 , H05K3/28 , H05K3/421 , H05K3/427 , H05K2201/09527 , Y10T29/49124 , Y10T29/49155 , Y10T29/49165 , Y10T156/10
摘要： Alternative approaches to fabricating printed circuit boards for use in droplet actuator operations are provided. In one embodiment, a method of manufacturing a droplet actuator for conducting droplet operations includes positioning a dielectric material between a first metal layer configured to include an electrode and a second metal layer configured to include an interconnect pad. The method additionally includes forming a connection between the first and second metal layers. Droplet actuators and methods of fabricating and supporting printed circuit boards of droplet actuators are also provided.
摘要翻译： 提供制造用于液滴致动器操作的印刷电路板的替代方法。 在一个实施例中，制造用于进行液滴操作的液滴致动器的方法包括将介电材料定位在被配置为包括电极的第一金属层和被配置为包括互连焊盘的第二金属层之间。 该方法还包括在第一和第二金属层之间形成连接。 还提供了液滴致动器和制造和支持液滴致动器的印刷电路板的方法。
-
公开(公告)号：US08460528B2
公开(公告)日：2013-06-11
申请号：US12681879
申请日：2008-10-14
IPC分类号： G01N27/26
CPC分类号： B01L3/5027 , B01J2219/00371 , B01J2219/00378 , B01J2219/00385 , B01J2219/00515 , B01J2219/00518 , B01J2219/0065 , B01J2219/00653 , B01L3/502715 , B01L3/502792 , B01L2200/027 , B01L2200/0605 , B01L2200/0668 , B01L2200/16 , B01L2300/0816 , B01L2300/0819 , B01L2300/0845 , B01L2300/089 , B01L2400/0427 , B01L2400/0478 , B01L2400/0487
摘要： On-board reagent storage, handling, and dispensing apparatuses and methods for droplet actuator systems are provided. In one embodiment, a fluidic analyzer is provided and includes a droplet actuator including a substrate including a plurality of electrodes configured to actuate a droplet and a reagent storage component in selective fluid communication with the droplet actuator and including a reagent configured to combine with a solvent at a time of use. In another embodiment, a method of conducting fluidic analysis is provided and includes storing a reagent within a reagent storage component in fluid communication with a droplet actuator including a plurality of electrodes configured to actuate a droplet and combining the reagent with a solvent at a time for use within the droplet actuator.
摘要翻译： 提供了用于液滴致动器系统的机载试剂储存，处理和分配装置和方法。 在一个实施例中，提供了一种流体分析器，其包括液滴致动器，该液滴致动器包括基底，该基底包括配置成致动液滴的多个电极和与液滴致动器选择性流体连通的试剂储存部件，并且包括配置成与溶剂组合的试剂 在使用时。 在另一个实施例中，提供了一种进行流体分析的方法，包括将试剂储存在与液滴致动器流体连通的液体致动器内，所述液滴致动器包括配置成致动液滴的多个电极并且一次将溶剂与溶剂组合 在液滴致动器内使用。
-
公开(公告)号：US08268246B2
公开(公告)日：2012-09-18
申请号：US12531794
申请日：2008-08-11
CPC分类号： H05K3/4038 , B01L3/502707 , B01L3/502784 , B01L3/502792 , B01L2300/089 , B01L2300/12 , B01L2400/0427 , H05K1/113 , H05K3/0058 , H05K3/0067 , H05K3/28 , H05K3/421 , H05K3/427 , H05K2201/09527 , Y10T29/49124 , Y10T29/49155 , Y10T29/49165 , Y10T156/10
摘要： Alternative approaches to fabricating printed circuit boards for use in droplet actuator operations are provided. In one embodiment, a method of manufacturing a droplet actuator for conducting droplet operations includes positioning a dielectric material between a first metal layer configured to include an electrode and a second metal layer configured to include an interconnect pad. The method additionally includes forming a connection between the first and second metal layers. Droplet actuators and methods of fabricating and supporting printed circuit boards of droplet actuators are also provided.
摘要翻译： 提供制造用于液滴致动器操作的印刷电路板的替代方法。 在一个实施例中，制造用于进行液滴操作的液滴致动器的方法包括将介电材料定位在被配置为包括电极的第一金属层和被配置为包括互连焊盘的第二金属层之间。 该方法还包括在第一和第二金属层之间形成连接。 还提供了液滴致动器和制造和支持液滴致动器的印刷电路板的方法。
-
-
-
-
-
-
-
-
-
搜索结果
121 条记录 (耗时 0.046 秒)
