公开(公告)号：US12234572B2

公开(公告)日：2025-02-25

申请号：US17294807

申请日：2019-11-19

Applicant: UNIVERSITY OF WASHINGTON

Inventor： Alfredo Quijano Rubio ,  Daniel Adriano Silva Manzano ,  David Baker ,  Umut Ulge ,  Marc Joseph Lajoie

IPC: C40B40/10 ,  A61K38/20 ,  A61P35/00 ,  A61P37/04 ,  C07K14/54 ,  C07K14/55 ,  A61K48/00

Abstract: Conditionally active receptor agonists that, when activated, bind to IL-2 receptor βc heterodimer (IL-2Rβc), IL-4 receptor αc heterodimer (IL-4Rαc), or IL-13 receptor a subunit (IL˜13 Rα) are disclosed, as are components of the conditionally active receptor agonists and methods for using the conditionally active receptor agonists.

公开(公告)号：US12227553B2

公开(公告)日：2025-02-18

申请号：US18187639

申请日：2023-03-21

Applicant: UNIVERSITY OF WASHINGTON ,  THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY

Inventor： Daniel Adriano Silva Manzano ,  Shawn Yu ,  Umut Ulge ,  David Baker ,  Kenan Christopher Garcia ,  Jamie Spangler ,  Carl Walkey

IPC: C07K14/55 ,  A61K38/00 ,  A61K47/60 ,  A61K47/62 ,  C07K14/54

Abstract: De novo designed polypeptides that bind to IL-2 receptor βγc heterodimer (IL-2Rβγc), IL-4 receptor αγc heterodimer (IL-4Rαγc), or IL-13 receptor α subunit (IL-13Rα) are disclosed, as are methods for using and designing the polypeptides.

公开(公告)号：US20250051407A1

公开(公告)日：2025-02-13

申请号：US18799107

申请日：2024-08-09

Applicant: University of Washington

Inventor： David Baker ,  Cameron Glasscock ,  Robert Pecoraro ,  Ryan McHugh ,  Christoffer Norn ,  Frank DiMaio ,  David Benjamin Turitz Cox ,  Brian Coventry ,  Hugh Haddox ,  Gyu Rie Lee

IPC: C07K14/47 ,  C12N15/70

Abstract: Polypeptides are provided that include an amino acid sequence at least 50% identical, not including any amino acid insertions at identified insertion sites, to the amino acid sequence selected from the group consisting of SEQ ID NO: 1-52, wherein the polypeptide is a sequence-specific DNA-binding polypeptide, fusion proteins comprising such polypeptides, nucleic acids encoding such polypeptides and fusion proteins, and expression vectors and host cells comprising such nucleic acids.

公开(公告)号：US20240384221A1

公开(公告)日：2024-11-21

申请号：US18658657

申请日：2024-05-08

Applicant: University of Washington

Inventor： David Baker ,  Lukas F. Milles ,  Basile I.M. Wicky

IPC: C12M1/36 ,  B01D15/34 ,  C07K1/16 ,  C12N15/70 ,  C12P21/00 ,  G01N35/00 ,  G01N35/10

Abstract: Provided are systems, modules, compositions, and methods comprising integrated and modular workflows effective for high-throughput production and characterization of target polypeptides cost-effective protein production on the milligram scale, enabling characterization of hundreds of designs per day in a standardized format.

公开(公告)号：US20240337655A1

公开(公告)日：2024-10-10

申请号：US18597315

申请日：2024-03-06

Applicant: Trustees of Tufts College ,  University of Washington

Inventor： Fiorenzo G. Omenetto ,  Luciana d'Amone ,  Giuseppina Matzeu ,  Alfredo Quijano Rubio ,  David Baker

IPC: G01N33/566 ,  G01N33/569 ,  G01N33/573 ,  G01N33/74

CPC classification number: G01N33/566 ,  G01N33/56983 ,  G01N33/573 ,  G01N33/74 ,  G01N2333/43526

Abstract: A solid form sensing article can have one or more silk fibroin matrix sensing units, each having a solid form sensing property. Each of the one or more silk fibroin matrix sensing units can include a silk fibroin matrix. A plurality of Cage proteins and a plurality of Key proteins can be entrained within the silk fibroin matrix. The Cage proteins can bind to an analyte of interest.

公开(公告)号：US20240101615A1

公开(公告)日：2024-03-28

申请号：US18158384

申请日：2023-01-23

Applicant: UNIVERSITY OF WASHINGTON

Inventor： Zibo CHEN ,  Scott Boyken ,  Sherry Bermeo ,  Robert A. Langan ,  David Baker

IPC: C07K14/435 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y15/00 ,  C07K14/00 ,  C07K19/00 ,  G01N33/53 ,  G06N3/00 ,  G16B5/00 ,  G16B15/00 ,  H03K19/00

CPC classification number: C07K14/435 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y15/00 ,  C07K14/00 ,  C07K19/00 ,  G01N33/53 ,  G06N3/002 ,  G16B5/00 ,  G16B15/00 ,  H03K19/00 ,  C07K2319/00 ,  G16B5/10

Abstract: Disclosed herein are designed heterodimer proteins, monomeric polypeptides capable of forming heterodimer proteins, protein scaffolds including such polypeptides, and methods for using the heterodimer proteins and subunit polypeptides for designing logic gates.

公开(公告)号：US11802141B2

公开(公告)日：2023-10-31

申请号：US17078319

申请日：2020-10-23

Applicant: UNIVERSITY OF WASHINGTON

Inventor： Tamuka Chidyausiku ,  Enrique Marcos ,  Lucas Nivon ,  Gustav Oberdorfer ,  David Baker ,  Lauren Carter

IPC: C07K14/47 ,  C12N15/11 ,  C07K17/00

CPC classification number: C07K14/47 ,  C07K17/00 ,  C12N15/111

Abstract: Beta-sheet forming polypeptides at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identical to the amino acid sequence selected from the group consisting of SEQ ID NOS:1-24 are disclosed, together with their use and methods for designing beta-sheet forming polypeptides.

公开(公告)号：US11771755B2

公开(公告)日：2023-10-03

申请号：US16971278

申请日：2019-02-28

Applicant: UNIVERSITY OF WASHINGTON

Inventor： Neil King ,  David Baker ,  Lance Stewart ,  Brooke Fiala ,  Daniel Ellis ,  Lauren Carter ,  Rashmi Ravichandran ,  George Ueda ,  Jorge Fallas ,  Una Nattermann

IPC: A61K39/12 ,  C12N15/86 ,  B82Y5/00 ,  B82Y40/00

CPC classification number: A61K39/12 ,  C12N15/86 ,  B82Y5/00 ,  B82Y40/00 ,  C12N2710/16134 ,  C12N2710/16234 ,  C12N2760/00034

Abstract: Nanostructures and nanostructure-based vaccines that display antigens capable of eliciting immune responses to infectious agents such as bacteria, viruses, and pathogens are provided. Some vaccines are useful for preventing or decreasing the severity of infection with an infectious agent, including, for example and without limitation, lyme disease, pertussis, herpes virus, orthomyxovirus, paramyxovirus, pneumovirus, filovirus, flavivirus, reovirus, retrovirus, meningococcus, or malaria. The antigens may be attached to the core of the nanostructure either non-covalently or covalently, including as a fusion protein or by other means. Multimeric antigens may optionally be displayed along a symmetry axis of the nanostructure. Also provided are proteins and nucleic acid molecules encoding such proteins, vaccine compositions, and methods of administration.

公开(公告)号：US20210403578A1

公开(公告)日：2021-12-30

申请号：US17469661

申请日：2021-09-08

Applicant: The Board of Trustees of the Leland Stanford Junior University ,  University of Washington

Inventor： Kenan Christopher Garcia ,  David Baker ,  Claudia Yvonne Janda ,  Luke Dang ,  James Daniel Moody

IPC: C07K16/28 ,  C07K16/30

Abstract: Wnt signaling agonist compositions and methods for their use are provided. Wnt signaling agonists of the invention comprise a frizzled binding moiety, which is fused or conjugated to an LRP5 or LRP6 binding moiety.

公开(公告)号：US10988759B2

公开(公告)日：2021-04-27

申请号：US16856506

申请日：2020-04-23

Applicant: University of Washington

Inventor： David Baker ,  David Younger ,  Eric Klavins

IPC: C12N15/10

Abstract: The present invention relates to methods and compositions for the high throughput screening of protein-protein interactions in yeast liquid culture. Protein fusions non-native to yeast may be expressed to replace endogenous sexual agglutination proteins and mediate library-by-library interrogation of protein interactions. The methods and compositions of the invention can be utilized for the characterization of protein interaction networks in high throughput for both binding affinity and specificity, which is crucial for understanding cellular functions, screening therapeutic candidates, and evaluating engineered protein networks.