公开(公告)号：US20150361458A1

公开(公告)日：2015-12-17

申请号：US14740554

申请日：2015-06-16

申请人： INVISTA North America S.á r.l.

发明人： Adriana Leonora Botes ,  Alex Van Eck Conradie

IPC分类号： C12P7/62 ,  C12P13/00 ,  C12P7/42 ,  C12N9/18 ,  C12P11/00 ,  C12N9/16 ,  C12N9/10 ,  C12N9/02 ,  C12P7/44 ,  C12P7/18

CPC分类号： C12P7/62 ,  C07C69/533 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/16 ,  C12N9/18 ,  C12P7/18 ,  C12P7/24 ,  C12P7/40 ,  C12P7/42 ,  C12P7/44 ,  C12P11/00 ,  C12P13/001 ,  C12P13/005 ,  C12P17/10 ,  C12Y102/99006 ,  C12Y203/01016 ,  C12Y206/01018 ,  C12Y206/01019 ,  C12Y206/01029 ,  C12Y206/01048 ,  C12Y206/01082 ,  C12Y301/01085 ,  C12Y301/02

摘要： This document describes biochemical pathways for producing 2(E)-heptenedioyl-CoA methyl ester from precursors such as 2-oxo-glutarate, acetyl-CoA, or succinyl-CoA using one or more of a fatty acid O-methyltransferase, a thioesterase, a CoA-transferase, a CoA ligase, as well as recombinant hosts expressing one or more of such enzymes. 2(E)-heptenedioyl-CoA methyl ester can be enzymatically converted to pimeloyl-CoA using a trans-2-enoyl-CoA reductase, and a methylesterase. Pimeloyl-CoA can be enzymatically converted to pimelic acid, 7-aminoheptanoate, 7-hydroxyheptanoate, heptamethylenediamine, or 1,7-heptanediol.

摘要翻译： 本文件描述了使用一种或多种脂肪酸O-甲基转移酶，硫酯酶，硫酸酯酶等从前体例如2-氧代 - 戊二酸，乙酰辅酶A或琥珀酰-CoA制备2（E） - 庚二酰辅酶A甲酯的生化途径， CoA转移酶，CoA连接酶，以及表达一种或多种这样的酶的重组宿主。 2（E） - 庚二酰辅酶A甲酯可以使用反式-2-烯酰辅酶A还原酶和甲酯酶酶转化为双甲酰基-CoA。 可将Pimeloyl-CoA酶促转化成庚二酸，7-氨基庚酸酯，7-羟基庚酸酯，七亚甲基二胺或1,7-庚二醇。

公开(公告)号：US20150353960A1

公开(公告)日：2015-12-10

申请号：US14737261

申请日：2015-06-11

申请人： Ebio, LLC

发明人： Trent Nguyen ,  Martin J. Van Sickels

IPC分类号： C12P7/06 ,  B01D61/36 ,  B01D3/00 ,  B01D3/40

CPC分类号： C12P7/065 ,  B01D3/001 ,  B01D3/40 ,  B01D61/364 ,  C12N1/38 ,  C12N15/70 ,  C12P5/026 ,  C12P7/04 ,  C12P7/06 ,  C12P7/16 ,  C12P7/18 ,  C12P7/28 ,  C12P7/40 ,  C12P7/56 ,  C12P7/6409 ,  Y02E50/10 ,  Y02E50/17

摘要： Increasing the production of a glycolytic intermediate and the production of an organic compound, such as ethanol, by a bacteria expressing a polyglutamine protein is achieved by preparing a feed stream; combining the bacteria and feed stream; fermenting the feed stream; cooling the feed stream during fermentation; recovering the organic compound; and concentrating the organic compound. The feed stream can be a waste stream from an ethanol production facility. The process can allow for a similar yield to the ethanol plant alone but with the use of less feed stock, such as corn, sugarcane, sorghum, cassava, switchgrass, and wood chips.

摘要翻译： 通过制备进料流来实现增加糖酵解中间体的产生和由表达多聚谷氨酰胺蛋白质的细菌生产有机化合物如乙醇的方法; 结合细菌和饲料流; 发酵饲料流; 在发酵期间冷却进料流; 回收有机化合物; 并浓缩有机化合物。 进料流可以是来自乙醇生产设备的废物流。 该方法可以单独使用与乙醇装置相似的收率，但是可以使用较少的原料，例如玉米，甘蔗，高粱，木薯，柳枝稷和木片。

公开(公告)号：US20150344916A1

公开(公告)日：2015-12-03

申请号：US14575927

申请日：2014-12-18

申请人： OPX Biotechnologies, Inc. ,  The Regents of the University of Colorado

发明人： Michael D. Lynch ,  Ryan T. Gill ,  Tanya E.W. Lipscomb

IPC分类号： C12P7/62

CPC分类号： C12P11/00 ,  A61L15/24 ,  C12M47/12 ,  C12N9/0008 ,  C12N9/12 ,  C12N9/16 ,  C12N15/63 ,  C12P7/40 ,  C12P7/42 ,  C12P7/52 ,  C12P7/62 ,  C12Y102/04001 ,  C12Y207/00 ,  C12Y301/02 ,  Y02P20/52 ,  C08L33/08

摘要： This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a chemical product, which includes 3-hydroxypropionic acid.

摘要翻译： 本发明涉及代谢工程化的微生物菌株，例如细菌菌株，其中丙二酰辅酶A用于生产化学产品（其包括3-羟基丙酸）的利用率增加。

公开(公告)号：US20150299749A1

公开(公告)日：2015-10-22

申请号：US14440658

申请日：2013-11-07

申请人： DSM IP ASSETS B.V.

发明人： Bertus Noordam ,  Michael Petrus Jozef Berkhout ,  Aida Hiseni

IPC分类号： C12P19/02 ,  C12P19/14

CPC分类号： C12P7/10 ,  C12P5/026 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/48 ,  C12P7/54 ,  C12P7/56 ,  C12P17/10 ,  C12P19/02 ,  C12P19/14 ,  C12P35/00 ,  C12P2203/00 ,  Y02E50/16 ,  Y02E50/343

摘要： The invention relates to a process for the preparation of a sugar product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment of the ligno-cellulosic material; b) optionally washing of the optionally pre-treated ligno-cellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; and d) optionally recovery of a sugar product; wherein during part of the time of the enzymatic hydrolysis, oxygen is added to the ligno-cellulosic material and during part of the time of the enzymatic hydrolysis less oxygen is added to the ligno-cellulosic material compared to the other part of the time of the enzymatic hydrolysis, preferably no oxygen is added to the ligno-cellulosic material.

摘要翻译： 本发明涉及一种从木质纤维素材料制备糖产品的方法，包括以下步骤：a）任选预处理木素纤维素材料; b）任选地洗涤任选预处理的木质纤维素材料; c）使用包含至少两种纤维素酶的酶组合物对任选洗涤和/或任选预处理的木质纤维素材料进行酶水解，由此所述酶组合物至少包含GH61; 和d）任选地回收糖产品; 其中在酶水解的部分时间期间，将氧添加到木质纤维素材料中，并且在酶水解的部分时间期间，与木质纤维素材料的另一部分相比，较少的氧被加入到木质纤维素材料中 酶水解，优选不向木质纤维素材料中加入氧。

公开(公告)号：US20150291987A1

公开(公告)日：2015-10-15

申请号：US14418543

申请日：2013-09-20

申请人： BioAmber Inc.

发明人： Man Kit Lau

IPC分类号： C12P13/00 ,  C12N9/04 ,  C12P7/18 ,  C12N9/02 ,  C12N9/16 ,  C12P7/44 ,  C12N9/10 ,  C12N9/88

CPC分类号： C12P13/001 ,  C12N9/0006 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/13 ,  C12N9/16 ,  C12N9/88 ,  C12P7/18 ,  C12P7/40 ,  C12P7/44 ,  C12P13/005 ,  C12Y101/01 ,  C12Y103/01 ,  C12Y103/08 ,  C12Y206/01 ,  C12Y208/03 ,  C12Y301/02 ,  C12Y402/01

摘要： Recombinant microorganisms comprising at least one exogenous nucleic acid sequence and capable of producing adipate semialdehyde are provided. Adipate semialdehyde may be produced in a synthesis pathway utilizing a single thiolase reaction. Adipate semialdehyde may also be produced from intermediates consisting of alpha, omega difunctional aliphatic organic molecules. Methods of using recombinant microorganisms to produce 6-aminocaproic acid, adipic acid, hexamethylenediamine and 1.6-hexanediol are also provided.

摘要翻译： 提供了包含至少一种外源核酸序列并能够产生己二酸半醛的重组微生物。 己二酸半醛可以通过利用单一硫解酶反应的合成途径产生。 己二酸半醛也可以由由α，ω-二官能脂族有机分子组成的中间体产生。 还提供了使用重组微生物生产6-氨基己酸，己二酸，六亚甲基二胺和1.6-己二醇的方法。

公开(公告)号：US20150275239A1

公开(公告)日：2015-10-01

申请号：US14673004

申请日：2015-03-30

申请人： Samsung Electronics Co., Ltd.

发明人： Jiae Yun ,  Jieun Kim ,  Soonchun Chung ,  Joonsong Park ,  Jinhwan Park ,  Wooyong Lee ,  Kwangmyung Cho

IPC分类号： C12P7/40 ,  C12N15/77

CPC分类号： C12P7/40 ,  C07K14/34 ,  C12N9/1241 ,  C12P7/46 ,  C12Y207/07059

摘要： A genetically engineered bacteria cell having an enhanced activity of GlnD or GlnK, and a method of producing succinic acid by using the genetically engineered bacteria cell are provided.

摘要翻译： 提供具有增强的GlnD或GlnK活性的遗传工程细菌细胞，以及通过使用遗传工程改造的细菌细胞产生琥珀酸的方法。

公开(公告)号：US20150275191A1

公开(公告)日：2015-10-01

申请号：US14739232

申请日：2015-06-15

申请人： RIKEN

发明人： Toshiya MURANAKA ,  Hikaru Seki ,  Kiyoshi Ohyama ,  Hiroshi Sudo ,  Satoru Sawai ,  Kazuki Saito

IPC分类号： C12N9/02 ,  C12P15/00

CPC分类号： C12N9/0073 ,  C12N9/0006 ,  C12P7/40 ,  C12P15/00 ,  C12P33/00 ,  C12Y114/13134

摘要： Identification of a protein having an activity of oxidizing oleanane-type triterpene, and a gene encoding the protein, the protein and the gene, and use thereof are provided. For example, a protein having an activity of oxidizing oleanane-type triterpene obtained from a plant in the family Fabaceae, a gene encoding the protein and use thereof are provided. The protein is shown in, for example, SEQ ID NO: 4, 14 or 18, and the gene encoding the protein is shown in, for example, SEQ ID NO: 3, 13 or 17. A transformant into which the gene is introduced can be produced, and thereby a triterpene oxidase can be obtained.

摘要翻译： 提供具有氧化烯丙醇型三萜烯的活性的蛋白质和编码蛋白质，蛋白质和基因的基因的鉴定及其用途。 例如，提供了从豆科科植物获得的具有氧化烯丙醇型三萜的活性的蛋白质，编码该蛋白质的基因及其用途。 该蛋白质显示于例如SEQ ID NO：4,14或18中，编码该蛋白质的基因例如显示于SEQ ID NO：3,13或17中。引入基因的转化体 可以得到三萜氧化酶。

公开(公告)号：US09120061B2

公开(公告)日：2015-09-01

申请号：US12517518

申请日：2007-12-19

申请人： Werner Koschuh ,  Stefan Kromus

发明人： Werner Koschuh ,  Stefan Kromus

IPC分类号： B01D61/44 ,  B01D61/42 ,  B01D61/02 ,  B01D61/58 ,  C12P7/40 ,  C12P7/56 ,  C12P7/42

CPC分类号： B01D61/44 ,  B01D61/025 ,  B01D61/027 ,  B01D61/422 ,  B01D61/58 ,  B01D2317/025 ,  B03D2203/003 ,  C12P7/40 ,  C12P7/42 ,  C12P7/56

摘要： A process for a stream of substances containing at least one valuable substance including (A) amino acids, (B) carboxylic acids, and (C) inorganic salts includes: (1) treating the stream by nanofiltration to obtain a retentate enriched with valuable substance (A); (2) treating the permeate of step (1) by electrodialysis in order to obtain a concentrate enriched with valuable substance (C); (3) treating the diluate of step (2) using a system (3) of two stages (4) and (5) which are directly or indirectly interconnected, wherein (4) one treatment is performed by reverse osmosis and (5) one treatment is performed by electrodialysis, whereby a concentrate enriched with valuable substance (B) is obtained, (6) at least a portion of the retentate of step (4) is directly or indirectly supplied to step (5) and (7) at least a portion of the diluate of step (5) is directly or indirectly supplied to step (4).

摘要翻译： 含有（A）氨基酸，（B）羧酸和（C）无机盐的至少一种有价值物质的物质流的方法包括：（1）通过纳滤处理流以获得富含有价物质的滞留物 （一个）; （2）通过电渗析处理步骤（1）的渗透物，以获得富含有价值物质（C）的浓缩物; （3）使用直接或间接相连的两个阶段（4）和（5）的系统（3）处理步骤（2）的稀释物，其中（4）通过反渗透进行一次处理，和（5）一 通过电渗析进行处理，由此获得富含有价值物质（B）的浓缩物，（6）步骤（4）的至少一部分滞留物直接或间接地直接或间接地供应至步骤（5）和（7） 将步骤（5）的一部分稀释物直接或间接地供应到步骤（4）。

公开(公告)号：US09109241B2

公开(公告)日：2015-08-18

申请号：US14169174

申请日：2014-01-31

申请人： Xyleco, Inc.

发明人： Marshall Medoff ,  Thomas Craig Masterman

IPC分类号： C12N11/02 ,  C12P19/02 ,  C07H3/02 ,  C07H3/04 ,  C12N1/20 ,  C12P7/06 ,  C12P5/02 ,  C12P7/04 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/52 ,  C12P7/54 ,  C12P7/56 ,  C12P7/58 ,  C12P7/64

CPC分类号： C12P7/10 ,  C07H3/02 ,  C07H3/04 ,  C12N1/20 ,  C12N1/38 ,  C12P5/02 ,  C12P7/04 ,  C12P7/06 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/52 ,  C12P7/54 ,  C12P7/56 ,  C12P7/58 ,  C12P7/6409 ,  C12P19/02 ,  C12P2201/00 ,  C12P2203/00 ,  Y02E50/16 ,  Y02E50/17 ,  Y02E50/343

摘要： Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to proceed ethanol and/or butanol, e.g., by fermentation.

摘要翻译： 加工生物质（例如，植物生物量，动物生物质和城市废物生物质）以产生有用的产物，例如燃料。 例如，系统可以使用原料，例如纤维素和/或木质纤维素材料，例如通过发酵来进行乙醇和/或丁醇。

公开(公告)号：US20150218601A1

公开(公告)日：2015-08-06

申请号：US14624713

申请日：2015-02-18

申请人： Achim MARX ,  Markus Poetter ,  Stefan Buchholz ,  Alexander May ,  Hermann Siegert ,  Georg Fuchs ,  Birgit Alber ,  Lothar Eggeling

发明人： Achim MARX ,  Markus Poetter ,  Stefan Buchholz ,  Alexander May ,  Hermann Siegert ,  Georg Fuchs ,  Birgit Alber ,  Lothar Eggeling

IPC分类号： C12P7/42 ,  C12N9/88 ,  C08F120/10 ,  C12N9/10 ,  C08F120/06 ,  C12N9/16 ,  C12N9/02

CPC分类号： C12P7/42 ,  C08F120/06 ,  C08F120/10 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1096 ,  C12N9/16 ,  C12N9/88 ,  C12P7/40 ,  C12P7/52 ,  C12P7/625 ,  C12Y102/04004 ,  C12Y103/99012 ,  C12Y206/01042 ,  C12Y301/02004 ,  C12Y402/01017

摘要： The present invention relates to a cell which has been modified in comparison with its wild type in such a way that it is capable of forming more, by comparison with its wild, 3-hydroxyisobutyric acid or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid via methylmalonate-semialdehyde or 3-hydroxybutyryl-coenzyme A as precursors. The invention also relates to a method of generating a genetically modified cell, to the genetically modified cell obtainable by these methods, to a method of producing 3-hydroxyisobutyric acid or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid, to a method of producing methacrylic acid or methacrylic esters, and to a method of producing polymethacrylic acid or polymethacrylic esters. The present invention furthermore relates to an isolated DNA, to a vector, to the use of this vector for transforming a cell, to a transformed cell, and to a polypeptide.

摘要翻译： 本发明涉及与其野生型相比已被修饰的细胞，使得其能够通过与其野生型3-羟基异丁酸或基于3-羟基异丁酸的聚羟基链烷酸通过甲基丙二酸酯 - 半醛或3-羟基丁酰辅酶A作为前体。 本发明还涉及生产基于3-羟基异丁酸的3-羟基异丁酸或聚羟基链烷酸酯的生产方法，该方法可以通过这些方法产生遗传修饰的细胞，生产甲基丙烯酸或其制备方法 甲基丙烯酸酯，以及生产聚甲基丙烯酸或聚甲基丙烯酸酯的方法。 本发明还涉及分离的DNA，载体，使用该载体转化细胞，转化细胞和多肽。