DNA encoding plant chitinases
    73.
    发明授权
    DNA encoding plant chitinases 失效
    编码植物几丁质酶的DNA

    公开(公告)号:US5880328A

    公开(公告)日:1999-03-09

    申请号:US455736

    申请日:1995-05-31

    摘要: The present invention provides chemically regulatable DNA sequences capable of regulating transcription of an associated DNA sequence in plants or plant tissues, chimeric constructions containing such sequences, vectors containing such sequences and chimeric constructions, and transgenic plants and plant tissues containing these chimeric constructions. In one aspect, the chemically regulatable DNA sequences of the invention are derived from the 5' region of genes encoding pathogenisis-related (PR) proteins. The present invention also provides anti-pathogenic sequences derived from novel cDNAs coding for PR proteins which can be genetically engineered and transformed into plants to confer enhanced resistance to disease. Also provided is a method for the exogenous regulation of gene expression in plants, which comprises obtaining a plant incapable of regulating at least one gene or gene family, or at least one heterologous gene, due to the deactivation of at least one endogenous signal transduction cascade which regulates the gene in the plant, and applying a chemical regulator to the plant at a time when expression of the gene is desired. A novel signal peptide sequence and corresponding DNA coding sequence is also provided. Further provided are assays for the identification and isolation of additional chemically regulatable DNA sequences and cDNAs encoding PR proteins and assays for identifying chemicals capable of exogenously regulating the chemically regulatable DNA sequences of the invention.

    摘要翻译: 本发明提供能够调节植物或植物组织中相关DNA序列转录的化学可调节DNA序列,含有此类序列的嵌合构建体,含有此类序列的载体和嵌合构建体,以及含有这些嵌合构建体的转基因植物和植物组织。 在一个方面,本发明的化学可调节DNA序列衍生自编码发病相关(PR)蛋白的基因的5'区域。 本发明还提供衍生自编码PR蛋白的新cDNA的抗病原性序列,其可以被遗传工程化并转化成植物以赋予增强的抗病性。 还提供了外源调节植物中基因表达的方法,其包括获得不能调节至少一个基因或基因家族或至少一个异源基因的植物,因为至少一种内源性信号转导级联失活 其调节植物中的基因,并且在需要表达基因的时候将化学调节剂施用于植物。 还提供了新的信号肽序列和相应的DNA编码序列。 进一步提供用于鉴定和分离另外的化学可调节DNA序列和编码PR蛋白的cDNA和用于鉴定能够外源调节本发明的化学可调节DNA序列的化学品的测定。

    DNA encoding enzyme recombinant DNA and enzyme transformant, and their
preparation and uses
    74.
    发明授权
    DNA encoding enzyme recombinant DNA and enzyme transformant, and their preparation and uses 失效
    DNA编码酶重组DNA和酶转化体,及其制备及应用

    公开(公告)号:US5871977A

    公开(公告)日:1999-02-16

    申请号:US714677

    申请日:1996-09-16

    CPC分类号: C12P19/12 C12N9/90

    摘要: A DNA encoding an enzyme, which forms non-reducing saccharides having trehalose structure as an end unit from amylaceous saccharides having a degree of glucose polymerization of 3 or higher, enables an industrial-scale production of a recombinant enzyme with such enzyme activity. Non-reducing saccharides obtainable by the recombinant enzyme can be used in a variety of food products, cosmetics, pharmaceuticals and feeds because of their substantial non-reducibility, mild and high-quality sweetness, adequate viscosity, and moisture-retaining ability.

    摘要翻译: 从葡萄糖聚合度为3以上的淀粉糖形成具有海藻糖结构的非还原性糖类作为末端单元的酶的DNA能够实现具有这种酶活性的重组酶的工业规模生产。 可以通过重组酶获得的非还原性糖可以用于各种食品,化妆品,药​​物和饲料中,因为它们具有显着的不可还原性,温和和高品质的甜度,足够的粘度和保湿能力。