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公开(公告)号：US07816121B2
公开(公告)日：2010-10-19
申请号：US11639990
申请日：2006-12-15
CPC分类号： B01L3/502761 , B01L3/5025 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2200/0673 , B01L2200/147 , B01L2300/0819 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0427 , B01L2400/043 , B01L2400/0442 , B01L2400/0448 , B01L2400/0487 , G01N15/1484 , G01N2015/1006 , G01N2035/1046 , Y10T29/49002 , Y10T137/0318
摘要： A droplet actuation system is provided including a droplet actuation device including a substrate that includes electrodes for conducting droplet operations; temperature control means for heating and/or cooling a region of the droplet actuation device and arranged such that a droplet can be transported on the electrodes to a region for heating; and a means for effecting a magnetic field in proximity to one or more of the electrodes sufficient to immobilize magnetically responsive beads in a droplet on the substrate during droplet operations. The system further includes a processor for controlling the electrodes and temperature control means, wherein the processor is programmed, and electrodes and magnetic field are arranged, to cause the electrodes to split a droplet including the magnetically responsive beads yielding a first daughter droplet which includes the magnetically responsive beads and a second daughter droplet without a substantial amount of the magnetically responsive beads.
摘要翻译： 提供了一种液滴致动系统，其包括液滴致动装置，其包括包括用于进行液滴操作的电极的基板; 温度控制装置，用于加热和/或冷却液滴致动装置的区域，并布置成使得液滴可以在电极上传送到加热区域; 以及用于在一些或多个电极处进行磁场的装置，其足以在液滴操作期间将磁响应珠固定在基板上的液滴中。 该系统还包括用于控制电极和温度控制装置的处理器，其中处理器被编程，并且布置电极和磁场，以使电极分裂包括磁响应珠的液滴，产生包含第一子液滴的第一子液滴 磁响应珠和第二子液滴，而没有大量的磁响应珠。
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公开(公告)号：US20090280475A1
公开(公告)日：2009-11-12
申请号：US11639710
申请日：2006-12-15
CPC分类号： C12Q1/68 , B01L7/52 , B01L2300/0645 , B01L2300/1822 , B01L2300/1894 , B01L2400/0424 , B01L2400/0427 , C12Q1/6869 , G01N27/44791 , Y10T436/25 , C12Q2565/629 , C12Q2565/301 , C12Q2563/149
摘要： The present invention relates to a droplet microactuator and to systems, apparatuses and methods employing the droplet microactuator for executing various protocols using droplets. The invention includes a droplet microactuator or droplet microactuator system having one or more input reservoirs loaded with reagents for conducting sequencing protocols, such as the reagents for conducting a pyrosequencing protocol. The invention also includes a droplet microactuator or droplet microactuator system, having one or more input reservoirs loaded with a sample for conducting a pyrosequencing protocol.
摘要翻译： 液滴微致动器技术领域本发明涉及液滴微致动器以及采用液滴微致动器来使用液滴执行各种协议的系统，装置和方法。 本发明包括液滴微致动器或液滴微致动器系统，其具有一个或多个输入储存器，其中装载有用于进行测序方案的试剂，例如用于进行焦磷酸测序方案的试剂。 本发明还包括液滴微致动器或液滴微致动器系统，其具有装载有用于进行焦磷酸测序方案的样品的一个或多个输入储存器。
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公开(公告)号：US08287711B2
公开(公告)日：2012-10-16
申请号：US11965206
申请日：2007-12-27
IPC分类号： F04B19/00
CPC分类号： B01L3/50273 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01F13/0084 , B01L3/00 , B01L3/5027 , B01L3/502784 , B01L3/502792 , B01L2200/06 , B01L2200/0605 , B01L2200/10 , B01L2300/089 , B01L2300/161 , B01L2300/165 , B01L2400/0415 , B01L2400/0427 , B41J2002/14395 , C25B9/00 , G01N27/44791 , G02B26/005
摘要： An apparatus for manipulating droplets is provided. In one embodiment, the apparatus includes a substrate having a set of electrical leads for connecting electrodes to a controller, a first set of electrodes, each connected to a separate one of the electrical leads, and a second set of electrodes, all connected to a single one of the electrical leads. In another embodiment, the apparatus includes a substrate having a set of X electrodes, and a set of Y electrical leads, each connected to one or more electrodes, wherein X is greater than Y.
摘要翻译： 提供了一种用于操纵液滴的装置。 在一个实施例中，该装置包括具有用于将电极连接到控制器的一组电引线的基板，分别连接到电引线的单独电极的第一组电极和全部连接到 单一电引线。 在另一个实施例中，该装置包括具有一组X电极的基板和一组Y电引线，每组连接到一个或多个电极，其中X大于Y.
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公开(公告)号：US08147668B2
公开(公告)日：2012-04-03
申请号：US11585618
申请日：2006-10-23
IPC分类号： B03C5/02
CPC分类号： B01F13/0071 , B01F13/0076 , B01F13/0084 , B01J2219/00479 , B01J2219/00653 , B01L3/502792 , B01L2300/089 , B01L2300/165 , B01L2400/0415 , B01L2400/0427 , C40B60/14 , G01N15/14 , G01N2035/1046 , G02B26/005 , Y10T436/11 , Y10T436/113332 , Y10T436/117497 , Y10T436/118339 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要： Apparatuses for mixing droplets, such as a binary mixing apparatus, are provided. The binary mixing apparatus includes an array of electrodes and a conducting element positioned in relation to at least one of the electrodes to enable a droplet placed in electrical communication with the at least one electrode to electrically communicate with the conducting element. The binary mixing apparatus additionally includes an electrode selector for sequentially biasing one or more selected electrodes of the array to move a droplet disposed on the array into contact with another droplet. The apparatus further includes a first droplet supply area communicating with the array and a second droplet supply area communicating with the array.
摘要翻译： 提供了用于混合液滴的装置，例如二元混合装置。 二进制混合装置包括电极阵列和相对于至少一个电极定位的导电元件，使得与至少一个电极电连通的液滴能够与导电元件电连通。 二进制混合装置还包括用于顺序地偏置阵列的一个或多个所选择的电极的电极选择器，以移动设置在阵列上的液滴与另一液滴接触。 该装置还包括与阵列连通的第一液滴供应区域和与阵列连通的第二液滴供应区域。
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公开(公告)号：US20110100823A1
公开(公告)日：2011-05-05
申请号：US12960583
申请日：2010-12-06
IPC分类号： G01N21/76 , G01N27/447
CPC分类号： C12Q1/68 , B01L7/52 , B01L2300/0645 , B01L2300/1822 , B01L2300/1894 , B01L2400/0424 , B01L2400/0427 , C12Q1/6869 , G01N27/44791 , Y10T436/25 , C12Q2565/629 , C12Q2565/301 , C12Q2563/149
摘要： The present invention relates to a droplet-based nucleic acid amplification apparatus and system. According to one embodiment, a droplet microactuator is provided made using a first substrate including a fluorescing material and including a detection region for detecting a fluorescence signal from a droplet, which detection region is coated with a light absorbing, low fluorescence or non-fluorescing material.
摘要翻译： 本发明涉及一种基于液滴的核酸扩增装置和系统。 根据一个实施例，使用包括荧光材料的第一基底并且包括用于检测来自液滴的荧光信号的检测区域来提供液滴微致动器，该检测区域被光吸收，低荧光或非荧光材料涂覆 。
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公开(公告)号：US20100025242A1
公开(公告)日：2010-02-04
申请号：US12576794
申请日：2009-10-09
IPC分类号： B01D57/02
CPC分类号： G01N27/44769 , B01F13/0071 , B01F13/0076 , B01L3/502707 , B01L3/502792 , B01L7/52 , B01L2200/0673 , B01L2200/10 , B01L2200/12 , B01L2300/0645 , B01L2300/0663 , B01L2300/0819 , B01L2300/0864 , B01L2300/0867 , B01L2300/0887 , B01L2300/089 , B01L2300/12 , B01L2300/1827 , B01L2400/0415 , B01L2400/0424 , B81B3/0021 , B81B2201/058 , B81C1/00119 , C12Q1/6846 , G01N27/447 , G01N27/44791 , G05D7/0694 , H01L21/6715 , Y10T436/2575
摘要： Apparatuses and methods for manipulating droplets are disclosed. In one embodiment, an apparatus for manipulating droplets is provided, the apparatus including a substrate, multiple arrays of electrodes disposed on the substrate, wherein corresponding electrodes in each array are connected to a common electrical signal, and a dielectric layer disposed on the substrate first side surface and patterned to cover the electrodes.
摘要翻译： 公开了用于操纵液滴的装置和方法。 在一个实施例中，提供了一种用于操纵液滴的装置，该装置包括一个基板，设置在该基板上的多个电极阵列，其中每个阵列中的对应电极连接到公共电信号，以及首先设置在基板上的电介质层 侧表面并图案化以覆盖电极。
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公开(公告)号：US07329545B2
公开(公告)日：2008-02-12
申请号：US10253342
申请日：2002-09-24
CPC分类号： B01F13/0071 , B01F13/0076 , B01F13/0084 , B01J2219/00479 , B01J2219/00653 , B01L3/502792 , B01L2300/089 , B01L2300/165 , B01L2400/0415 , B01L2400/0427 , C40B60/14 , G01N15/14 , G01N2035/1046 , G02B26/005 , Y10T436/11 , Y10T436/113332 , Y10T436/117497 , Y10T436/118339 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要： In a method for sampling a continuous liquid flow, the liquid flow is supplied to a surface along an input flow path. The liquid flow is sampled by forming a sample droplet from a portion of the liquid flow. The sample droplet is moved along an analysis flow path to a processing area of the surface, where the sample droplet is processed. Discrete sample droplets are formed and moved using an electrowetting technique. A binary mixing apparatus and method are also provided. The apparatus comprises an array of electrodes, an electronic controller communicating with the electrodes, a sample droplet supply area communicating with the array, and an additive droplet supply area communicating with the array. The electronic controller alternately energizes and de-energizes selected electrodes to carry out droplet-to-droplet binary mixing operations to obtain one or more droplets having a target mixing ratio.
摘要翻译： 在对连续液体流进行取样的方法中，液体流被输送到沿输入流路的表面。 通过从液体流的一部分形成样品液滴来取样液体流。 样品液滴沿着分析流动路径移动到表面的处理区域，在该处理区域处理样品液滴。 使用电润湿技术形成并移动离散样品液滴。 还提供了二元混合装置和方法。 该装置包括电极阵列，与电极连通的电子控制器，与该阵列连通的样品液滴供应区域以及与该阵列连通的附加液滴供应区域。 电子控制器交替地激励和去激活所选择的电极以进行液滴到液滴的二进制混合操作以获得具有目标混合比的一个或多个液滴。
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公开(公告)号：US09243282B2
公开(公告)日：2016-01-26
申请号：US13470919
申请日：2012-05-14
IPC分类号： C12Q1/68 , C12P19/34 , G01N21/00 , G01N27/447
CPC分类号： C12Q1/68 , B01L7/52 , B01L2300/0645 , B01L2300/1822 , B01L2300/1894 , B01L2400/0424 , B01L2400/0427 , C12Q1/6869 , G01N27/44791 , Y10T436/25 , C12Q2565/629 , C12Q2565/301 , C12Q2563/149
摘要： The present invention relates to droplet-based pyrosequencing including a method of identifying a base at a target position in a sample nucleic acid. The method includes: (a) providing a droplet microactuator including a first droplet including a sample nucleic acid immobilized on a bead; and (b) on the droplet microactuator: (i) contacting the first droplet with one or more reagent droplets to yield a second droplet, wherein the one or more reagent droplets include reagents for extending a double stranded portion of the sample nucleic acid by incorporating a nucleotide at the target position; (ii) splitting the second droplet to yield a third droplet including the bead and a fourth droplet lacking the bead; and (iii) assaying the third droplet to determine whether the nucleotide was incorporated at the target position.
摘要翻译： 本发明涉及基于液滴的焦磷酸测序，包括鉴定样品核酸中目标位置的碱基的方法。 该方法包括：（a）提供包括包含固定在珠粒上的样品核酸的第一液滴的液滴微致动器; 和（b）在所述液滴微致动器上：（i）使所述第一液滴与一个或多个试剂液滴接触以产生第二液滴，其中所述一个或多个试剂液滴包括用于通过掺入来扩展样品核酸的双链部分的试剂 目标位置的核苷酸; （ii）分裂第二液滴以产生包含珠粒的第三液滴和不含珠粒的第四液滴; 和（iii）测定第三液滴以确定核苷酸是否并入目标位置。
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公开(公告)号：US20130118901A1
公开(公告)日：2013-05-16
申请号：US13737433
申请日：2013-01-09
IPC分类号： G01N27/447
CPC分类号： G01N27/44769 , B01F13/0071 , B01F13/0076 , B01L3/502707 , B01L3/502792 , B01L7/52 , B01L2200/0673 , B01L2200/10 , B01L2200/12 , B01L2300/0645 , B01L2300/0663 , B01L2300/0819 , B01L2300/0864 , B01L2300/0867 , B01L2300/0887 , B01L2300/089 , B01L2300/12 , B01L2300/1827 , B01L2400/0415 , B01L2400/0424 , B81B3/0021 , B81B2201/058 , B81C1/00119 , C12Q1/6846 , G01N27/447 , G01N27/44791 , G05D7/0694 , H01L21/6715 , Y10T436/2575
摘要： Apparatuses and methods for manipulating droplets are disclosed. In one embodiment, an apparatus for manipulating droplets is provided, the apparatus including a substrate, multiple arrays of electrodes disposed on the substrate, wherein corresponding electrodes in each array are connected to a common electrical signal, and a dielectric layer disposed on the substrate first side surface and patterned to cover the electrodes.
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公开(公告)号：US20130092539A1
公开(公告)日：2013-04-18
申请号：US13680979
申请日：2012-11-19
IPC分类号： G01N27/447
CPC分类号： C12Q1/68 , B01L7/52 , B01L2300/0645 , B01L2300/1822 , B01L2300/1894 , B01L2400/0424 , B01L2400/0427 , C12Q1/6869 , G01N27/44791 , Y10T436/25 , C12Q2565/629 , C12Q2565/301 , C12Q2563/149
摘要： Aspects of embodiments of the invention relate to a simulator including a visual display capable of outputting to a user a display one or more effects of a command series selected and a system including a droplet microactuator electronically coupled to and controlled by a processor capable of executing instructions, the droplet microactuator including a substrate comprising electrodes for conducting droplet operations. Further aspects of embodiments of the invention relate to a droplet operations troubleshooting apparatus. Other aspects of embodiments of the invention relate to a computer implemented method of displaying simulated microactuator droplets.
摘要翻译： 本发明的实施例的方面涉及一种模拟器，其包括能够向用户输出显示所选择的命令序列的一个或多个效果的视觉显示器，以及包括电子耦合到能够执行指令的处理器并由其控制的液滴微致动器的系统 液滴微致动器包括包括用于进行液滴操作的电极的基板。 本发明的实施例的其它方面涉及一种液滴操作故障排除装置。 本发明的实施例的其他方面涉及一种显示模拟微致动器液滴的计算机实现的方法。
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