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1339 条记录 (耗时 0.042 秒)

    Mass spectrometric methods for biomolecular screening
    81.
    发明授权
    Mass spectrometric methods for biomolecular screening 有权
    用于生物分子筛选的质谱法

    公开(公告)号：US08211636B2

    公开(公告)日：2012-07-03

    申请号：US10608354

    申请日：2003-06-27

    申请人： Stanley T. Crook ,  Richard Griffey ,  Steven Hofstadler

    发明人： Stanley T. Crook ,  Richard Griffey ,  Steven Hofstadler

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  G01N24/00 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12Q1/6816 ,  B01J2219/00702 ,  B01J2219/00722 ,  C12Q1/6806 ,  C12Q1/6811 ,  C40B40/06 ,  H01J49/004 ,  Y10T436/24 ,  C12Q2565/627 ,  C12Q2525/101 ,  C12Q2565/133 ,  C12Q2525/121 ,  C12Q2565/607

    摘要： The present invention provides methods for the determination of the structure of biomolecular targets, as well as the site and nature of the interaction between ligands and biomolecular targets. The present invention also provides methods for the determination of the relative affinity of a ligand for the biomolecular target it interacts with. Also provided are methods for screening ligand or combinatorial libraries of compounds against one or more than one biological target molecules. The methods of the invention also allow determination of the relative binding affinity of combinatorial and other compounds for a biomolecular target. The present invention further provides methods for the use of mass modifying tags for screening multiple biomolecular targets. In a preferred embodiment, ligands which have great specificity and affinity for molecular interaction sites on biomolecules, especially RNA can be identified. In preferred embodiments, such identification can be made simultaneously with libraries of ligands.

    摘要翻译： 本发明提供了用于确定生物分子靶标的结构的方法，以及配体和生物分子靶标之间的相互作用的位点和性质。 本发明还提供了确定配体与其相互作用的生物分子靶的相对亲和力的方法。 还提供了用于筛选针对一种或多于一种生物靶分子的化合物的配体或组合文库的方法。 本发明的方法还允许确定组合和其它化合物对于生物分子靶标的相对结合亲和力。 本发明还提供使用质量修饰标签筛选多个生物分子靶标的方法。 在优选的实施方案中，可以鉴定对生物分子，特别是RNA上的分子相互作用位点具有极大特异性和亲和力的配体。 在优选的实施方案中，这种鉴定可以与配体文库同时进行。

    Methods of obtaining active antisense compounds
    82.
    发明授权
    Methods of obtaining active antisense compounds 有权
    获得活性反义化合物的方法

    公开(公告)号：US08202982B2

    公开(公告)日：2012-06-19

    申请号：US12557358

    申请日：2009-09-10

    申请人： Susan M. Freier ,  Olga Matveeva ,  Alexander Tsodikov ,  Michael C. Giddings ,  Jacqueline R. Wyatt

    发明人： Susan M. Freier ,  Olga Matveeva ,  Alexander Tsodikov ,  Michael C. Giddings ,  Jacqueline R. Wyatt

    IPC分类号： C07H21/00 ,  C07H21/04 ,  A61K31/70

    CPC分类号： C12N15/113 ,  C07H21/00 ,  C12Q1/6811

    摘要： Methods for obtaining antisense oligonucleotides with activity against a desired target are provided. Methods of identifying oligonucleotide sequence motifs which are predictive of antisense oligonucleotide activity are provided, as are motifs identified according to this method. Methods of selecting effective antisense oligonucleotide sequences and effective antisense target sequences are provided, as are sequences selected according to these methods. In other methods of the invention, oligonucleotides are designed to hybridize to target sequences containing one or more activity-enhancing motifs. Antisense oligonucleotides designed according to these methods are also provided.

    摘要翻译： 提供了获得具有针对所需靶标的活性的反义寡核苷酸的方法。 提供了鉴定预测反义寡核苷酸活性的寡核苷酸序列基序的方法，以及根据该方法鉴定的基序。 提供了选择有效的反义寡核苷酸序列和有效的反义靶序列的方法，以及根据这些方法选择的序列。 在本发明的其它方法中，设计寡核苷酸与含有一种或多种活性增强基序的靶序列杂交。 还提供了根据这些方法设计的反义寡核苷酸。

    Sperm cell separation methods and compositions containing aptamers or nucleic acid sequences for use therein
    84.
    发明授权
    Sperm cell separation methods and compositions containing aptamers or nucleic acid sequences for use therein 有权
    精子细胞分离方法和含有用于其中的适体或核酸序列的组合物

    公开(公告)号：US08138319B2

    公开(公告)日：2012-03-20

    申请号：US12394993

    申请日：2009-02-27

    申请人： David Oksenberg ,  Sergey Krylov ,  Michael Musheev

    发明人： David Oksenberg ,  Sergey Krylov ,  Michael Musheev

    IPC分类号： C07H21/02 ,  G01N33/53

    CPC分类号： C12Q1/6811 ,  C12N5/061 ,  C12N5/0612 ,  C12N15/115 ,  C12N2310/16 ,  C12N2320/10 ,  C12Q1/6879

    摘要： The present invention provides an aptamer or pool of aptamers (nucleic acid sequences) that bind(s) to a target molecule on the surface, accessible from the surface or inside of a mammalian sperm cell and a method for producing the aptamers. The method comprises contacting a collection of different nucleic acid molecules with the target molecule under conditions favorable for binding between at least one of the nucleic acid molecules and the target molecule, to form at least one complex comprising the nucleic acid molecule bound to the target molecule, wherein each of the nucleic acid molecules comprises at least one segment of randomized nucleotide sequences. The complexes are then separated from the unbound nucleic acid molecules and unbound target molecules, and the bound nucleic acid molecule is recovered from the separated complex. The aptamers are used to separate sperm cells based upon sperm cell qualities, such as whether the cells contain X chromosomes or Y chromosomes.

    摘要翻译： 本发明提供了适配体或核酸序列池，其与表面上的靶分子结合，可从哺乳动物精子细胞的表面或内部获得，以及用于制备适体的方法。 该方法包括在有利于至少一个核酸分子和靶分子之间的结合的条件下将不同核酸分子的集合与靶分子接触，以形成至少一个包含与靶分子结合的核酸分子的复合物 其中每个核酸分子包含至少一个随机化核苷酸序列片段。 然后将复合物与未结合的核酸分子和未结合的靶分子分离，并且从分离的复合物中回收结合的核酸分子。 适配体用于基于精子细胞质量分离精子细胞，例如细胞是否含有X染色体或Y染色体。

    REVERSE TRANSCRIPTION PRIMERS AND METHODS OF DESIGN
    86.
    发明申请
    REVERSE TRANSCRIPTION PRIMERS AND METHODS OF DESIGN 有权
    反向转录原型和设计方法

    公开(公告)号：US20110257039A1

    公开(公告)日：2011-10-20

    申请号：US12950179

    申请日：2010-11-19

    申请人： Xiaowei Wang ,  Xiaohui Wang ,  Robert Setterquist

    发明人： Xiaowei Wang ,  Xiaohui Wang ,  Robert Setterquist

    IPC分类号： C40B40/06 ,  C40B50/00

    CPC分类号： C12Q1/6811 ,  C12N15/1096 ,  C12Q1/6876 ,  C12Q2600/16 ,  C40B40/06 ,  C40B40/08 ,  G06F19/22

    摘要： The present invention provides novel algorithms for designing oligonucleotides that do not substantially hybridize to a small group of unwanted transcripts, while hybridizing to most other transcripts. Such oligonucleotides are particularly useful as primers for reverse transcription. The invention also provides compositions containing oligonucleotides that do not substantially hybridize to a small group of unwanted transcripts, while hybridizing to most other transcripts.

    摘要翻译： 本发明提供了用于设计寡核苷酸的新颖算法，所述寡核苷酸与大部分其他转录物杂交，基本上不与少量不想要的转录物杂交。 这样的寡核苷酸特别可用作逆转录的引物。 本发明还提供了含有寡核苷酸的组合物，其与大部分其他转录物杂交而基本上不与少量不想要的转录物杂交。

    Methods of analyzing chromosomal translocations using fluorescence in situ hybridization (FISH)
    89.
    发明授权
    Methods of analyzing chromosomal translocations using fluorescence in situ hybridization (FISH) 有权
    使用荧光原位杂交（FISH）分析染色体易位的方法

    公开(公告)号：US07964345B2

    公开(公告)日：2011-06-21

    申请号：US11100135

    申请日：2005-04-05

    申请人： Nallasivam Palanisamy ,  Raju S. Chaganti

    发明人： Nallasivam Palanisamy ,  Raju S. Chaganti

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04 ,  C07H21/00

    CPC分类号： C12Q1/6841 ,  C12Q1/6811 ,  C12Q1/6886 ,  C12Q2539/105

    摘要： Probes and methods of using the probes to detect chromosomal rearrangements and/or deletions are provided. The methods utilize probes that are free of repeat sequences to provide greater selectivity and sensitivity; methods for producing such probes are also disclosed. The probe sets utilized in the detection methods are designed to hybridize to chromosomes at regions outside known breakpoints, instead of spanning the breakpoint as with conventional FISH methods, and, in some instances, are further designed to bind to regions located outside the genes involved in the rearrangement. Methods utilizing probe sets with two and four colors are also described, as are automated methods for analyzing rearrangements.

    摘要翻译： 提供了使用探针检测染色体重排和/或缺失的探针和方法。 该方法利用无重复序列的探针提供更大的选择性和灵敏度; 还公开了生产这种探针的方法。 在检测方法中使用的探针组被设计为与已知断裂点之外的区域处的染色体杂交，而不是像常规FISH方法跨越断点，并且在一些情况下进一步设计为结合位于涉及的基因外部的区域 重排。 还描述了利用具有两种和四种颜色的探针组的方法，以及用于分析重排的自动化方法。