公开(公告)号：US12087797B2

公开(公告)日：2024-09-10

申请号：US18175836

申请日：2023-02-28

申请人： Illumina, Inc.

发明人： Xiuyu Cai ,  Ali Agah ,  Tracy H. Fung ,  Dietrich Dehlinger

IPC分类号： H01L27/146 ,  G01J1/02 ,  G01J1/44 ,  G01N21/05 ,  G01N21/64

CPC分类号： H01L27/14636 ,  G01J1/0209 ,  G01J1/0214 ,  G01J1/44 ,  G01N21/6454 ,  H01L27/14601 ,  H01L27/14609 ,  H01L27/1462 ,  H01L27/14625 ,  H01L27/14629 ,  H01L27/14643 ,  G01N21/05 ,  G01N2201/0642 ,  H01L27/14623

摘要： An example image sensor structure includes an image layer. The image layer includes an array of light detectors disposed therein. A device stack is disposed over the image layer. An array of light guides is disposed in the device stack. Each light guide is associated with at least one light detector of the array of light detectors. A passivation stack is disposed over the device stack. The passivation stack includes a bottom surface in direct contact with a top surface of the light guides. An array of nanowells is disposed in a top layer of the passivation stack. Each nanowell is associated with a light guide of the array of light guides. A crosstalk blocking metal structure is disposed in the passivation stack. The crosstalk blocking metal structure reduces crosstalk within the passivation stack.

公开(公告)号：US11874214B1

公开(公告)日：2024-01-16

申请号：US18179525

申请日：2023-03-07

申请人： Illumina, Inc.

发明人： Helmy A. Eltoukhy ,  Robert C. Kain ,  Wenyi Feng ,  Mark Pratt ,  Bernard Hirschbein ,  Poorya Sabounchi ,  Tarun Khurana

IPC分类号： G01N15/14 ,  C12Q1/6874 ,  B01L3/00

CPC分类号： G01N15/1434 ,  B01L3/502715 ,  C12Q1/6874 ,  G01N15/1404 ,  B01L2300/0654 ,  B01L2300/168

摘要： A biosensor is provided including a detection device and a flow cell mounted to the detection device. The detection device has a detector surface with a plurality of reaction sites. The detection device also includes a filter layer. A method is providing including obtaining signal data from an array of light detectors; determining a crosstalk function for each of the light detectors of the array of light detectors; and determining characteristics of analytes of interest based on the signal data using the crosstalk functions.

公开(公告)号：US11567060B2

公开(公告)日：2023-01-31

申请号：US16624567

申请日：2018-06-19

申请人： ILLUMINA, INC.

发明人： Boyan Boyanov ,  Rohan N. Akolkar ,  Jeffrey S. Fisher ,  Jeffrey G. Mandell ,  Liangliang Qiang ,  Steven M. Barnard

IPC分类号： G01N33/487 ,  C12Q1/6869 ,  G01N27/447

摘要： Example nanopore sequencers include a cis well, a trans well, and a nanopore fluidically connecting the cis and trans wells. In one example sequencer, a modified electrolyte (including an electrolyte and a cation complexing agent) is present in the cis well, or the trans well, or in the cis and the trans wells. In another example sequencer, a gel state polyelectrolyte is present in the cis well, or the trans well, or in the cis and the trans wells.

公开(公告)号：US11542550B2

公开(公告)日：2023-01-03

申请号：US16626425

申请日：2019-02-13

申请人： Illumina, Inc.

发明人： Jeffrey Mandell ,  Steven Barnard ,  John Moon ,  Maria Candelaria Rogert Bacigalupo

IPC分类号： C12Q1/68 ,  C07H19/06 ,  C12Q1/6869 ,  B01L3/00

摘要： A labeled nucleotide includes a nucleotide, a linking molecule attached to a phosphate group of the nucleotide, and a redox-active charge tag attached to the linking molecule. The redox-active charge tag is to be oxidized or reduced by an electrically conductive channel when maintained in proximity of a sensing zone of the electrically conductive channel.

公开(公告)号：US11525827B2

公开(公告)日：2022-12-13

申请号：US16811789

申请日：2020-03-06

申请人： Advanced Liquid Logic, Inc.

发明人： Vamsee K. Pamula ,  Michael G. Pollack ,  Ramakrishna Sista ,  Arjun Sudarsan

IPC分类号： G01N33/543 ,  B01L3/00 ,  B03C1/02 ,  B03C1/28 ,  B03C1/30 ,  B03C5/00 ,  C12N15/10 ,  G01N33/561 ,  B01F31/65 ,  B01F33/302 ,  B01F33/3031 ,  B01L7/00 ,  G01N35/10

摘要： Methods are provided for separating magnetically responsive beads from a droplet in a droplet actuator. Droplet operations electrodes and a magnet are arranged in a droplet actuator to manipulate a bead-containing droplet and position it relative to a magnetic field region that attracts the magnetically responsive beads. The droplet operations electrodes are operated to control the droplet shape and transport it away from the magnetic field region to form a concentration of beads in the droplet. The continued transport of the droplet away from the magnetic field causes the concentration of beads to break away from the droplet to yield a small, concentrated bead-containing droplet immobilized by the magnet.

公开(公告)号：US11249025B2

公开(公告)日：2022-02-15

申请号：US16482630

申请日：2018-01-31

申请人： Illumina, Inc.

发明人： John S. Vieceli ,  Alex Nemiroski ,  Paul Belitz ,  Robert Langlois ,  M. Shane Bowen ,  Danny Yuan Chan ,  Bala Murali K. Venkatesan ,  Hui Han ,  Kevan Samiee ,  Stephen Tanner

IPC分类号： G01N21/64

摘要： Fiducial markers are provided on a patterned array of the type that may be used for molecular analysis, such as sequencing. The fiducial markers may have configurations and layouts that enhance their detection in image or detection data, that facilitate or improve processing, that provide encoding of useful information, and so forth. Examples of the fiducial markers may include non-rectilinear layouts that may provide for more robust location of both the fiducial markers and sites of the patterned array.

公开(公告)号：US11174513B2

公开(公告)日：2021-11-16

申请号：US16405547

申请日：2019-05-07

申请人： ILLUMINA, INC.

发明人： Poorya Sabounchi ,  Behnam Javanmardi ,  Tarun Khurana ,  Philip Paik ,  Yan-You Lin

IPC分类号： B01L3/00 ,  B01L7/00 ,  C12Q1/6869 ,  C12Q1/6806 ,  C12Q1/68

摘要： A stack of fluidics layers of a microfluidic cartridge for sequencing nucleic acid molecules includes a sequencing chamber layer having a sequencing chamber area configured for carrying out clustering and sequencing reactions, and a sequencing chamber bottom layer disposed under the sequencing chamber layer. The sequencing chamber bottom layer has an opening configured to hold an image sensor with the image sensor having an active area disposed under the sequencing chamber area. The sequencing chamber area spans substantially all of the active area of the image sensor. The stack of fluidics layers includes a flexible printed circuit board (PCB) layer under the sequencing chamber bottom layer, and a fluidics channels layer disposed under the flexible PCB layer. The fluidics channels layer includes fluidics channels that are configured to deliver reactants to the sequencing chamber area. The fluidics channels do not substantially overlap with the active area of the image sensor.

公开(公告)号：US10909666B2

公开(公告)日：2021-02-02

申请号：US16192608

申请日：2018-11-15

申请人： Illumina, Inc.

发明人： Robert Langlois ,  Paul Belitz

IPC分类号： G06T5/00 ,  H04N5/357 ,  G01N21/64 ,  G02B21/00 ,  G06K9/00 ,  H04N1/401 ,  H04N1/409 ,  G01B11/16 ,  G06K9/32 ,  G06T3/00 ,  H04N1/387 ,  G06K9/36

摘要： Techniques are described for dynamically correcting image distortion during imaging of a patterned sample having repeating spots. Different sets of image distortion correction coefficients may be calculated for different regions of a sample during a first imaging cycle of a multicycle imaging run and subsequently applied in real time to image data generated during subsequent cycles. In one implementation, image distortion correction coefficients may be calculated for an image of a patterned sample having repeated spots by: estimating an affine transform of the image; sharpening the image; and iteratively searching for an optimal set of distortion correction coefficients for the sharpened image, where iteratively searching for the optimal set of distortion correction coefficients for the sharpened image includes calculating a mean chastity for spot locations in the image, and where the estimated affine transform is applied during each iteration of the search.

公开(公告)号：US10906044B2

公开(公告)日：2021-02-02

申请号：US15746390

申请日：2016-08-31

申请人： Illumina Cambridge Limited

发明人： Xavier Von Hatten

IPC分类号： B01L3/00 ,  C08L33/26 ,  C08L83/08 ,  C08L83/14

摘要： Embodiments of present application are directed to micro fluidic devices and particularly digital micro fluidic devices with improved droplet operations, and methods of improving droplet operations in micro fluidic devices.

公开(公告)号：US10857537B2

公开(公告)日：2020-12-08

申请号：US15739678

申请日：2016-07-05

申请人： ILLUMINA, INC.

发明人： Kirkpatrick W. Norton

IPC分类号： B01L3/00

摘要： A droplet actuator device for conducting droplet operations is provided that comprises a substrate defines a device channel to conduct droplet operations. Electrodes are arranged proximate to the substrate. A drive circuit is connected to the electrodes. The drive circuit generates an electrode drive signal to drive the droplet operations based on a reference waveform. The electrode drive signal is partitioned into an AC modulated drive cycle formed of sub-cycles. The electrode drive signal switches, during the sub-cycle, between at least first and second states where a degree of modulation with respect to the reference waveform forms a balanced modulation pattern.