公开(公告)号：US6025129A

公开(公告)日：2000-02-15

申请号：US567746

申请日：1995-12-05

申请人： Michael P. Nova ,  Andrew E. Senyei ,  Zahra Parandoosh ,  Gary S. David

发明人： Michael P. Nova ,  Andrew E. Senyei ,  Zahra Parandoosh ,  Gary S. David

IPC分类号： B01J19/00 ,  B01L3/00 ,  C07K1/00 ,  C07K1/04 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  C40B70/00 ,  G01N15/14 ,  G01N35/00 ,  G11C13/02 ,  C12Q1/68 ,  C07H21/04 ,  C12P19/34 ,  G01N33/53

CPC分类号： G11C13/0014 ,  B01J19/0046 ,  B82Y10/00 ,  C07K1/00 ,  C07K1/04 ,  C07K1/047 ,  G01N35/00732 ,  G01N35/00871 ,  G11C13/0019 ,  B01J2219/00295 ,  B01J2219/00299 ,  B01J2219/00308 ,  B01J2219/00333 ,  B01J2219/00344 ,  B01J2219/00461 ,  B01J2219/00463 ,  B01J2219/005 ,  B01J2219/00502 ,  B01J2219/0054 ,  B01J2219/00542 ,  B01J2219/00547 ,  B01J2219/00549 ,  B01J2219/00551 ,  B01J2219/0056 ,  B01J2219/00563 ,  B01J2219/00567 ,  B01J2219/00569 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00592 ,  B01J2219/00596 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L2300/0609 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  C40B70/00 ,  G01N2015/149 ,  G01N2035/00782 ,  G11C13/025

摘要： Combinations, called matrices with memories, of matrix materials with remotely addressable or remotely programmable recording devices that contain at least one data storage unit are provided. The matrix materials are those that are used in as supports in solid phase chemical and biochemical syntheses, immunoassays and hybridization reactions. The matrix materials may additionally include fluophors or other luminescent moieties to produce luminescing matrices with memories. The data storage units are non-volatile antifuse memories or volatile memories, such as EEPROMS, DRAMS or flash memory. By virtue of this combination, molecules and biological particles, such as phage and viral particles and cells, that are in proximity or in physical contact with the matrix combination can be labeled by programming the memory with identifying information and can be identified by retrieving the stored information. Combinations of matrix materials, memories, and linked molecules and biological materials are also provided. The combinations have a multiplicity of applications, including combinatorial chemistry, isolation and purification of target macromolecules, capture and detection of macromolecules for analytical purposes, selective removal of contaminants, enzymatic catalysis, cell sorting, drug delivery, chemical modification and other uses. Methods for electronically tagging molecules, biological particles and matrix support materials, immunoassays, receptor binding assays, scintillation proximity assays, non-radioactive proximity assays, and other methods are also provided.

摘要翻译： 提供了具有包含至少一个数据存储单元的具有可远程寻址或远程可编程记录设备的矩阵材料的具有存储器的组合。 基质材料是在固相化学和生物化学合成，免疫测定和杂交反应中用作载体的基质材料。 基质材料还可以包括荧光体或其它发光部分以产生具有记忆的发光基质。 数据存储单元是非易失性反熔丝存储器或易失性存储器，例如EEPROMS，DRAMS或闪存。 通过这种组合，可以通过用识别信息对存储器进行编程来标记与基质组合接近或物理接触的分子和生物颗粒，例如噬菌体和病毒颗粒和细胞，并且可以通过检索存储的 信息。 还提供了基质材料，记忆和连接分子和生物材料的组合。 这些组合具有多种应用，包括组合化学，目标大分子的分离和纯化，用于分析目的的大分子的捕获和检测，污染物的选择性去除，酶催化，细胞分选，药物递送，化学修饰和其它用途。 还提供了用于电子标记分子，生物颗粒和基质支持材料的方法，免疫测定，受体结合测定，闪烁亲近测定，非放射性邻近测定和其它方法。

公开(公告)号：US5925562A

公开(公告)日：1999-07-20

申请号：US480196

申请日：1995-06-07

申请人： Michael P. Nova ,  Andrew E. Senyei ,  Gary S. David

发明人： Michael P. Nova ,  Andrew E. Senyei ,  Gary S. David

IPC分类号： B01J19/00 ,  B01L3/00 ,  C07K1/00 ,  C07K1/04 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  C40B70/00 ,  G01N15/14 ,  G01N35/00 ,  G11C13/02 ,  G01N33/50 ,  G01B13/14 ,  G01N33/566

CPC分类号： G01N35/00732 ,  B01J19/0046 ,  B82Y10/00 ,  C07K1/00 ,  C07K1/04 ,  C07K1/047 ,  G01N35/00871 ,  G11C13/0014 ,  G11C13/0019 ,  B01J2219/00295 ,  B01J2219/00299 ,  B01J2219/00308 ,  B01J2219/00344 ,  B01J2219/00461 ,  B01J2219/00463 ,  B01J2219/005 ,  B01J2219/00502 ,  B01J2219/0054 ,  B01J2219/00542 ,  B01J2219/00549 ,  B01J2219/00551 ,  B01J2219/0056 ,  B01J2219/00567 ,  B01J2219/00569 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00592 ,  B01J2219/00596 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  C40B70/00 ,  G01N2015/149 ,  G01N2035/00782 ,  G11C13/025

摘要： Combinations, called matrices with memories, of matrix materials with remotely addressable or remotely programmable recording devices that contain at least one data storage unit are provided. The matrix materials are those that are used in as supports in solid phase chemical and biochemical syntheses, immunoassays and hybridization reactions. The data storage units are preferably non-volatile antifuse memories. By virtue of this combination, molecules and biological particles, such as phage and viral particles and cells, that are in proximity or in physical contact with the matrix combination can be labeled by programming the memory with identifying information and can be identified by retrieving the stored information. Combinations of matrix materials, memories, and linked molecules and biological materials are also provided. The combinations have a multiplicity of applications, including combinatorial chemistry, isolation and purification of target macromolecules, capture and detection of macromolecules for analytical purposes, selective removal of contaminants, enzymatic catalysis, chemical modification and other uses.

公开(公告)号：US4376110A

公开(公告)日：1983-03-08

申请号：US175133

申请日：1980-08-04

申请人： Gary S. David ,  Howard E. Greene

发明人： Gary S. David ,  Howard E. Greene

IPC分类号： G01N33/53 ,  G01N33/576 ,  G01N33/577 ,  G01N33/58 ,  G01N33/54 ,  G01N33/56

CPC分类号： G01N33/58 ,  G01N33/576 ,  G01N33/577 ,  Y10S435/971

摘要： "Two-site" or "sandwich" immunometric assay techniques for determination of the presence and/or concentration of antigenic substances in fluids using monoclonal antibodies. One monoclonal antibody is presented in a soluble labeled form and a second monoclonal antibody is presented bound to a solid carrier; the soluble and bound monoclonal antibodies may be the products of either the same or different cell lines. Each monoclonal antibody has an affinity for the antigenic substances of at least about 10.sup.8 liters/mole.

摘要翻译： 使用单克隆抗体测定流体中抗原物质的存在和/或浓度的“双位点”或“三明治”免疫测定技术。 一种单克隆抗体以可溶性标记形式存在，并且第二单克隆抗体被提供结合到固体载体上; 可溶性和结合的单克隆抗体可以是相同或不同细胞系的产物。 每种单克隆抗体对抗原物质具有至少约108升/摩尔的亲和力。

公开(公告)号：US06331273B1

公开(公告)日：2001-12-18

申请号：US08473660

申请日：1995-06-07

申请人： Michael P. Nova ,  Andrew E. Senyei ,  Gary S. David

发明人： Michael P. Nova ,  Andrew E. Senyei ,  Gary S. David

IPC分类号： G01N1506

CPC分类号： G11C13/0019 ,  B01J19/0046 ,  B01J2219/00295 ,  B01J2219/00299 ,  B01J2219/00308 ,  B01J2219/00344 ,  B01J2219/00461 ,  B01J2219/00463 ,  B01J2219/005 ,  B01J2219/00502 ,  B01J2219/0054 ,  B01J2219/00542 ,  B01J2219/00549 ,  B01J2219/00551 ,  B01J2219/0056 ,  B01J2219/00567 ,  B01J2219/00569 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00592 ,  B01J2219/00596 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B82Y10/00 ,  C07K1/00 ,  C07K1/04 ,  C07K1/047 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  C40B70/00 ,  G01N35/00732 ,  G01N35/00871 ,  G01N2015/149 ,  G01N2035/00782 ,  G11C13/0014 ,  G11C13/025

摘要： Combinations, called matrices with memories, of matrix materials with remotely addressable or remotely programmable recording devices that contain at least one data storage unit are provided. The matrix materials are those that are used in as supports in solid phase chemical and biochemical syntheses, immunoassays and hybridization reactions. The data storage units are preferably non-volatile antifuse memories. By virtue of this combination, molecules and biological particles, such as phage and viral particles and cells, that are in proximity or in physical contact with the matrix combination can be labeled by programming the memory with identifying information and can be identified by retrieving the stored information. Combinations of matrix materials, memories, and linked molecules and biological materials are also provided. The combinations have a multiplicity of applications, including combinatorial chemistry, isolation and purification of target macromolecules, capture and detection of macromolecules for analytical purposes, selective removal of contaminants, enzymatic catalysis, chemical modification and other uses. Methods for electronically tagging molecules are biological particles and matrix support materials and immunoassays and and other methods are also provided.

摘要翻译： 提供了具有包含至少一个数据存储单元的具有可远程寻址或远程可编程记录设备的矩阵材料的具有存储器的组合。 基质材料是在固相化学和生物化学合成，免疫测定和杂交反应中用作载体的基质材料。 数据存储单元优选地是非易失性反熔丝存储器。 通过这种组合，可以通过用识别信息对存储器进行编程来标记与基质组合接近或物理接触的分子和生物颗粒，例如噬菌体和病毒颗粒和细胞，并且可以通过检索存储的 信息。 还提供了基质材料，记忆和连接分子和生物材料的组合。 这些组合具有多种应用，包括组合化学，目标大分子的分离和纯化，用于分析目的的大分子的捕获和检测，污染物的选择性去除，酶催化，化学修饰和其它用途。 用于电子标记分子的方法是生物颗粒和基质支持材料和免疫测定法，并且还提供了其它方法。

公开(公告)号：US4722892A

公开(公告)日：1988-02-02

申请号：US646602

申请日：1984-08-31

申请人： Claude F. Meares ,  Gary S. David

发明人： Claude F. Meares ,  Gary S. David

IPC分类号： G01N33/53 ,  A61K38/00 ,  A61K39/00 ,  A61K39/395 ,  A61K51/00 ,  A61K51/10 ,  C07K14/005 ,  C07K14/195 ,  C07K16/00 ,  C07K16/44 ,  C07K16/46 ,  C12N15/00 ,  C12N15/02 ,  C12P1/00 ,  C12P21/00 ,  C12P21/08 ,  G01N33/534 ,  G01N33/577

CPC分类号： A61K51/1093 ,  C07K16/44 ,  C07K16/468 ,  A61K2121/00 ,  A61K38/00 ,  Y10S436/813 ,  Y10S530/808

摘要： Monoclonal Antibodies which are specific for a complex of a chelating agent and a metallic ion are described. The antibody has an association constant (K.sub.a) for the complex which is at least about ten times greater than the K.sub.a for the chelating agent alone or its complex with another metal.

摘要翻译： 描述了对螯合剂和金属离子络合物特异的单克隆抗体。 该抗体对于复合物具有缔合常数（Ka），其比单独的螯合剂的Ka或其与另一种金属的复合物的Ka大至少约十倍。

公开(公告)号：US08219327B2

公开(公告)日：2012-07-10

申请号：US13099198

申请日：2011-05-02

申请人： Michael P. Nova ,  Andrew E. Senyei ,  Zahra Parandoosh ,  Gary S. David ,  Xiao-Yi Xiao

发明人： Michael P. Nova ,  Andrew E. Senyei ,  Zahra Parandoosh ,  Gary S. David ,  Xiao-Yi Xiao

IPC分类号： G01N33/48 ,  G01N33/50 ,  G01N31/00 ,  C12Q1/00

CPC分类号： C40B30/04 ,  B01J19/0046 ,  B01J2219/00295 ,  B01J2219/00299 ,  B01J2219/00308 ,  B01J2219/00333 ,  B01J2219/00344 ,  B01J2219/00461 ,  B01J2219/00463 ,  B01J2219/005 ,  B01J2219/00502 ,  B01J2219/0054 ,  B01J2219/00542 ,  B01J2219/00547 ,  B01J2219/00549 ,  B01J2219/00551 ,  B01J2219/0056 ,  B01J2219/00563 ,  B01J2219/00567 ,  B01J2219/00569 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00592 ,  B01J2219/00596 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/5027 ,  B01L3/502707 ,  B01L2300/0609 ,  B82Y10/00 ,  C07K1/00 ,  C07K1/04 ,  C07K1/047 ,  C40B40/06 ,  C40B40/10 ,  C40B60/08 ,  C40B60/14 ,  C40B70/00 ,  G01N35/00732 ,  G01N35/00871 ,  G01N2015/149 ,  G01N2035/00782 ,  G11C13/0014 ,  G11C13/0019 ,  G11C13/025

摘要： In a multiplexed assay, each molecule of a plurality of molecules is attached to a support matrix with a substrate adapted for attachment and/or synthesis of molecules and an integrally-formed memory device with an optically-encoded identifier to uniquely identify the molecule attached to the substrate. The molecules are exposed to one or more processing conditions then placed within the path of an optical detector adapted to read the optically-encoded identifier and measure biochemical processes on each support matrix. The support matrices may besingulated to be read by the optical detector one at a time.

摘要翻译： 在多重分析中，多个分子的每个分子连接到具有适于附着和/或合成分子的底物的支持基质和具有光学编码标识符的整体形成的存储器件，以唯一地识别附着于 底物。 分子暴露于一个或多个处理条件，然后放置在适于读取光学编码标识符并测量每个支撑矩阵上的生物化学过程的光学检测器的路径内。 支撑矩阵可以一次一个地被光学检测器读取。

公开(公告)号：US06319668B1

公开(公告)日：2001-11-20

申请号：US08669252

申请日：1996-06-24

申请人： Michael P. Nova ,  Hanan Potash ,  Xiao-Yi Xiao ,  Zahra Parandoosh ,  Gary S. David

发明人： Michael P. Nova ,  Hanan Potash ,  Xiao-Yi Xiao ,  Zahra Parandoosh ,  Gary S. David

IPC分类号： C12Q168

CPC分类号： G11C13/0019 ,  B01J19/0046 ,  B01J2219/00295 ,  B01J2219/00299 ,  B01J2219/00308 ,  B01J2219/00333 ,  B01J2219/00344 ,  B01J2219/00461 ,  B01J2219/00463 ,  B01J2219/005 ,  B01J2219/00502 ,  B01J2219/0054 ,  B01J2219/00542 ,  B01J2219/00547 ,  B01J2219/00549 ,  B01J2219/00551 ,  B01J2219/0056 ,  B01J2219/00563 ,  B01J2219/00567 ,  B01J2219/00569 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/50825 ,  B01L3/5453 ,  B01L2300/022 ,  B01L2300/024 ,  B01L2300/0609 ,  B82Y10/00 ,  C07K1/00 ,  C07K1/04 ,  C07K1/047 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  C40B70/00 ,  G01N35/00732 ,  G01N35/00871 ,  G01N2015/149 ,  G01N2035/00782 ,  G11C13/0014 ,  G11C13/025

摘要： Combinations, called matrices with memories, of matrix materials that are encoded with an optically readable code are provided. The matrix materials are those that are used in as supports in solid phase chemical and biochemical syntheses, immunoassays and hybridization reactions. The matrix materials may additionally include fluophors or other luminescent moieties to produce luminescing matrices with memories. The memories include electronic and optical storage media and also include optical memories, such as bar codes and other machine-readable codes. By virtue of this combination, molecules and biological particles, such as phage and viral particles and cells, that are in proximity or in physical contact with the matrix combination can be labeled by programming the memory with identifying information and can be identified by retrieving the stored information. Combinations of matrix materials, memories, and linked molecules and biological materials are also provided. The combinations have a multiplicity of applications, including combinatorial chemistry, isolation and purification of target macromolecules, capture and detection of macromolecules for analytical purposes, selective removal of contaminants, enzymatic catalysis, cell sorting, drug delivery, chemical modification and other uses. Methods for tagging molecules, biological particles and matrix support materials, immunoassays, receptor binding assays, scintillation proximity assays, non-radioactive proximity assays, and other methods are also provided.

摘要翻译： 提供了用光学可读代码编码的矩阵材料的组合，称为具有存储器的矩阵。 基质材料是在固相化学和生物化学合成，免疫测定和杂交反应中用作载体的基质材料。 基质材料还可以包括荧光体或其它发光部分以产生具有记忆的发光基质。 存储器包括电子和光学存储介质，并且还包括诸如条形码和其他机器可读代码的光学存储器。 通过这种组合，可以通过用识别信息对存储器进行编程来标记与基质组合接近或物理接触的分子和生物颗粒，例如噬菌体和病毒颗粒和细胞，并且可以通过检索存储的 信息。 还提供了基质材料，记忆和连接分子和生物材料的组合。 这些组合具有多种应用，包括组合化学，目标大分子的分离和纯化，用于分析目的的大分子的捕获和检测，污染物的选择性去除，酶催化，细胞分选，药物递送，化学修饰和其它用途。 还提供了用于标记分子，生物颗粒和基质支持材料的方法，免疫测定，受体结合测定，闪烁亲和测定，非放射性邻近测定和其它方法。

公开(公告)号：US06306975B1

公开(公告)日：2001-10-23

申请号：US09010951

申请日：1998-01-22

申请人： Chanfeng Zhao ,  John E. Lillig ,  Robert Neeper ,  Gordon W. Hudson ,  Anthony W. Czarnik ,  Zahra Parandoosh ,  Gary S. David ,  Xiao-Yi Xiao

发明人： Chanfeng Zhao ,  John E. Lillig ,  Robert Neeper ,  Gordon W. Hudson ,  Anthony W. Czarnik ,  Zahra Parandoosh ,  Gary S. David ,  Xiao-Yi Xiao

IPC分类号： C08F25900

CPC分类号： G01N33/54393 ,  B01J19/0046 ,  B01J2219/00283 ,  B01J2219/00295 ,  B01J2219/00299 ,  B01J2219/00308 ,  B01J2219/0031 ,  B01J2219/00313 ,  B01J2219/00319 ,  B01J2219/00344 ,  B01J2219/00454 ,  B01J2219/00461 ,  B01J2219/00463 ,  B01J2219/005 ,  B01J2219/00542 ,  B01J2219/00547 ,  B01J2219/00567 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00592 ,  B01J2219/00596 ,  B01J2219/00689 ,  B01J2219/00691 ,  B01J2219/00695 ,  B01L2300/0609 ,  B82Y10/00 ,  C07K1/047 ,  C08J7/16 ,  C08J2327/12 ,  C40B50/14 ,  C40B60/14 ,  C40B70/00 ,  G01N33/542 ,  G01N33/54353 ,  G01N2015/149 ,  G11C13/0014 ,  G11C13/0019 ,  G11C13/025 ,  G11C2213/81

摘要： Methods for irradiation induced graft polymerization of a monomers, such as styrenes, onto fluoropolymers are provided. The methods, which involve either the use of acids, preferably mineral acids, or creating a rough surface on the fluoropolymer, provide higher levels of grafting of the copolymer than grafting in the absence of the acid or of the rough surface on the fluoropolymer. Also provided are grafted copolymers produced by the methods. Methods for increasing the performance of solid phase assays, such as scintillation proximity assays, are also provided.

摘要翻译： 提供了将单体（如苯乙烯）接枝聚合到含氟聚合物上的辐射诱导方法。 涉及使用酸，优选无机酸，或在含氟聚合物上形成粗糙表面的方法提供了比在氟聚合物上不存在酸或粗糙表面时接枝更高水平的共聚物接枝。 还提供了通过该方法制备的接枝共聚物。 还提供了用于增加固相测定的性能的方法，例如闪烁亲近测定法。

公开(公告)号：US06017496A

公开(公告)日：2000-01-25

申请号：US709435

申请日：1996-09-06

申请人： Michael P. Nova ,  Zahra Parandoosh ,  Andrew E. Senyei ,  Xiao-Yi Xiao ,  Gary S. David ,  Yozo Satoda ,  Chanfeng Zhao ,  Hanan Potash

发明人： Michael P. Nova ,  Zahra Parandoosh ,  Andrew E. Senyei ,  Xiao-Yi Xiao ,  Gary S. David ,  Yozo Satoda ,  Chanfeng Zhao ,  Hanan Potash

IPC分类号： B01J19/00 ,  B01L3/00 ,  C07B61/00 ,  C07H21/00 ,  C07K1/00 ,  C07K1/04 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  C40B70/00 ,  G01N15/14 ,  G01N35/00 ,  G11C13/02 ,  G01N15/06 ,  G01N33/48 ,  G01N35/02 ,  G01N35/04

CPC分类号： G11C13/0019 ,  B01J19/0046 ,  B01L3/50825 ,  B01L3/5453 ,  B82Y10/00 ,  C07H21/00 ,  C07K1/00 ,  C07K1/04 ,  C07K1/047 ,  G01N35/00732 ,  G01N35/00871 ,  G11C13/0014 ,  B01J2219/00283 ,  B01J2219/00295 ,  B01J2219/00299 ,  B01J2219/00308 ,  B01J2219/00333 ,  B01J2219/00344 ,  B01J2219/00461 ,  B01J2219/00463 ,  B01J2219/005 ,  B01J2219/00502 ,  B01J2219/0054 ,  B01J2219/00542 ,  B01J2219/00547 ,  B01J2219/00549 ,  B01J2219/00551 ,  B01J2219/00554 ,  B01J2219/0056 ,  B01J2219/00563 ,  B01J2219/00567 ,  B01J2219/00569 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00592 ,  B01J2219/00596 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L2300/022 ,  B01L2300/0609 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  C40B70/00 ,  G01N2015/149 ,  G01N2035/00782 ,  G01N2333/9121 ,  G01N2500/00 ,  G11C13/025

摘要： Combinations, called matrices with memories, of matrix materials that are encoded with an optically readable code are provided. The matrix materials are those that are used in as supports in solid phase chemical and biochemical syntheses, immunoassays and hybridization reactions. The matrix materials may additionally include fluophors or other luminescent moieties to produce luminescing matrices with memories. The memories include electronic and optical storage media and also include optical memories, such as bar codes and other machine-readable codes. By virtue of this combination, molecules and biological particles, such as phage and viral particles and cells, that are in proximity or in physical contact with the matrix combination can be labeled by programming the memory with identifying information and can be identified by retrieving the stored information. Combinations of matrix materials, memories, and linked molecules and biological materials are also provided. The combinations have a multiplicity of applications, including combinatorial chemistry, isolation and purification of target macromolecules, capture and detection of macromolecules for analytical purposes, selective removal of contaminants, enzymatic catalysis, cell sorting, drug delivery, chemical modification and other uses. Methods for tagging molecules, biological particles and matrix support materials, immunoassays, receptor binding assays, scintillation proximity assays, non-radioactive proximity assays, and other methods are also provided.

公开(公告)号：US4486530A

公开(公告)日：1984-12-04

申请号：US323498

申请日：1981-06-24

申请人： Gary S. David ,  Howard E. Greene

发明人： Gary S. David ,  Howard E. Greene

IPC分类号： G01N33/542 ,  G01N33/543 ,  G01N33/576 ,  G01N33/577 ,  G01N33/54 ,  G01N33/56

CPC分类号： G01N33/577 ,  G01N33/542 ,  G01N33/54313 ,  G01N33/576 ,  Y10S436/80

摘要： "Two-site" or "sandwich" immunometric assay techniques for determination of the presence and/or concentration of antigenic substances in fluids using monoclonal antibodies are described and compared to conventional assays using polyclonal antibodies. Also described are inhibition assays using complexes of antigens with a monoclonal antibody.

摘要翻译： 描述使用单克隆抗体测定流体中抗原物质的存在和/或浓度的“双位点”或“三明治”免疫测定技术，并与使用多克隆抗体的常规测定进行比较。 还描述了使用抗原与单克隆抗体的复合物的抑制测定。