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12 条记录 (耗时 0.02 秒)

    Heterocyclic compounds and their use for isolating nucleic acids
    1.
    发明授权
    Heterocyclic compounds and their use for isolating nucleic acids 有权
    杂环化合物及其分离核酸的用途

    公开(公告)号：US06403786B1

    公开(公告)日：2002-06-11

    申请号：US09254644

    申请日：1999-07-02

    申请人： Klaus Mühlegger ,  Herbert Von der Eltz

    发明人： Klaus Mühlegger ,  Herbert Von der Eltz

    IPC分类号： C07H1910

    CPC分类号： C07D405/04 ,  C07D413/04 ,  C07F9/65586 ,  C07H7/06 ,  C07H19/04 ,  C07H19/048 ,  C07H19/06 ,  C07J51/00

    摘要： The invention concerns compounds of the general formula (I) in which i:he residues R1 to R7 have the meanings given in the application as well as methods for their preparation. The compounds are in particular suitable as substrates for RNA or DNA polymerases and can thus be incorporated into RNA or DNA oligonucleotides and are especially suitable for labelling and detecting nucleic acids or for DNA sequencing.

    摘要翻译： 本发明涉及通式（I）的化合物，其中I：残基R1至R7具有本申请中给出的含义及其制备方法。 该化合物特别适合作为RNA或DNA聚合酶的底物，因此可以并入RNA或DNA寡核苷酸，特别适用于标记和检测核酸或进行DNA测序。

    C-nucleoside derivatives and their use in the detection of nucleic acids
    3.
    发明授权
    C-nucleoside derivatives and their use in the detection of nucleic acids 失效
    C-核苷衍生物及其在核酸检测中的应用

    公开(公告)号：US06174998B1

    公开(公告)日：2001-01-16

    申请号：US08929068

    申请日：1997-09-15

    申请人： Klaus M{umlaut over (u)}hlegger ,  Herbert Von der Eltz ,  Frank Seela ,  Helmet Rosemeyer

    发明人： Klaus M{umlaut over (u)}hlegger ,  Herbert Von der Eltz ,  Frank Seela ,  Helmet Rosemeyer

    IPC分类号： C07G1100

    CPC分类号： C07H19/06 ,  C07H21/00

    摘要： The invention concerns pyrrolo-[3,2-d]pyrimidine, pyrazolo-[4,3-d]pyrimidine and pyrimidine-furanosides i.e. so-called C-nucleosides of the general formulae I-V or appropriate derivatives as well as processes for their production. The compounds are in particular suitable as substrates for RNA or DNA polymerases and can thus be incorporated into RNA or DNA oligonucleotides. Therefore the compounds are especially suitable for labelling and for detecting nucleic acids and for DNA sequencing.

    摘要翻译： 本发明涉及吡咯并[3,2-d]嘧啶，吡唑并[4,3-d]嘧啶和嘧啶 - 呋喃糖苷，即所谓的通式I-V的C-核苷和合适的衍生物，以及它们 生产。 这些化合物特别适用于RNA或DNA聚合酶的底物，因此可以并入RNA或DNA寡核苷酸。 因此，该化合物特别适用于标记和检测核酸和DNA测序。

    Measurement of vitamin D
    6.
    发明授权
    Measurement of vitamin D 有权
    维生素D的测定

    公开(公告)号：US08003400B2

    公开(公告)日：2011-08-23

    申请号：US12326972

    申请日：2008-12-03

    申请人： Uwe Kobold ,  Thomas Duelffer ,  Michael Grol ,  Rupert Herrmann ,  Herbert Von der Eltz ,  Leopold Von Proff

    发明人： Uwe Kobold ,  Thomas Duelffer ,  Michael Grol ,  Rupert Herrmann ,  Herbert Von der Eltz ,  Leopold Von Proff

    IPC分类号： G01N33/00

    CPC分类号： G01N33/82 ,  Y10T436/20 ,  Y10T436/203332

    摘要： The present invention relates to a method of measuring a vitamin D metabolite in a sample, the method comprising the steps of (a) treating said sample with a vitamin D metabolite releasing reagent under conditions appropriate to release a vitamin D metabolite from vitamin D-binding protein and not to cause protein precipitation, (b) subjecting the treated sample obtained in step (a) to a chromatographic separation, and (c) measuring a vitamin D metabolite during or after said chromatographic separation. The present invention also relates to methods for determining the vitamin D status of a subject, for use in the diagnosis of disease, and to agents and kits for use in performing the methods of the invention.

    摘要翻译： 本发明涉及一种测量样品中维生素D代谢物的方法，所述方法包括以下步骤：（a）在适于从维生素D结合物释放维生素D代谢物的条件下用维生素D代谢物释放试剂处理所述样品 蛋白质，不会引起蛋白质沉淀，（b）使步骤（a）中得到的处理样品进行色谱分离，和（c）在所述色谱分离期间或之后测量维生素D代谢物。 本发明还涉及确定用于疾病诊断的受试者的维生素D状态的方法以及用于实施本发明的方法的试剂和试剂盒。

    Isolation and purification of nucleic acids with a solid phase
    9.
    发明授权
    Isolation and purification of nucleic acids with a solid phase 有权
    分离和纯化具有固相的核酸

    公开(公告)号：US08101744B2

    公开(公告)日：2012-01-24

    申请号：US12105556

    申请日：2008-04-18

    申请人： Christian Birkner ,  Herbert Von der Eltz

    发明人： Christian Birkner ,  Herbert Von der Eltz

    IPC分类号： C07H21/00

    CPC分类号： C12N15/1006

    摘要： Disclosed are water-soluble ionic liquids suitable for promoting adsorption of nucleic acids to a solid phase. The use thereof, particularly methods for the isolation of nucleic acids from an aqueous solution, as well as kits for performing those methods are disclosed.

    摘要翻译： 公开了适用于促进核酸吸附到固相的水溶性离子液体。 公开了其用途，特别是用于从水溶液中分离核酸的方法，以及用于实施这些方法的试剂盒。

    MEASUREMENT OF VITAMIN D
    10.
    发明申请
    MEASUREMENT OF VITAMIN D 有权
    维生素D的测定

    公开(公告)号：US20100285603A1

    公开(公告)日：2010-11-11

    申请号：US12326972

    申请日：2008-12-03

    申请人： Uwe Kobold ,  Thomas Duelffer ,  Michael Grol ,  Rupert Herrmann ,  Herbert Von der Eltz ,  Leopold Von Proff

    发明人： Uwe Kobold ,  Thomas Duelffer ,  Michael Grol ,  Rupert Herrmann ,  Herbert Von der Eltz ,  Leopold Von Proff

    IPC分类号： G01N33/00

    CPC分类号： G01N33/82 ,  Y10T436/20 ,  Y10T436/203332

    摘要： The present invention relates to a method of measuring a vitamin D metabolite in a sample, the method comprising the steps of (a) treating said sample with a vitamin D metabolite releasing reagent under conditions appropriate to release a vitamin D metabolite from vitamin D-binding protein and not to cause protein precipitation, (b) subjecting the treated sample obtained in step (a) to a chromatographic separation, and (c) measuring a vitamin D metabolite during or after said chromatographic separation. The present invention also relates to methods for determining the vitamin D status of a subject, for use in the diagnosis of disease, and to agents and kits for use in performing the methods of the invention.

    摘要翻译： 本发明涉及一种测量样品中维生素D代谢物的方法，所述方法包括以下步骤：（a）在适于从维生素D结合物释放维生素D代谢物的条件下用维生素D代谢物释放试剂处理所述样品 蛋白质，不会引起蛋白质沉淀，（b）使步骤（a）中得到的处理样品进行色谱分离，和（c）在所述色谱分离期间或之后测量维生素D代谢物。 本发明还涉及确定用于疾病诊断的受试者的维生素D状态的方法以及用于实施本发明的方法的试剂和试剂盒。