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    Apparatus for detecting nucleic acids using bead and nanopore
    1.
    发明授权
    Apparatus for detecting nucleic acids using bead and nanopore 失效
    用珠和纳米孔检测核酸的装置

    公开(公告)号：US08431337B2

    公开(公告)日：2013-04-30

    申请号：US11499052

    申请日：2006-08-04

    申请人： Kui Hyun Kim ,  Jun Hong Min ,  Ah Gi Kim ,  In Ho Lee

    发明人： Kui Hyun Kim ,  Jun Hong Min ,  Ah Gi Kim ,  In Ho Lee

    IPC分类号： C12Q1/68 ,  C12Q1/00 ,  C12M1/34

    CPC分类号： B82Y30/00 ,  C12Q1/6816 ,  C12Q2563/149 ,  C12Q2525/131 ,  C12Q2521/514

    摘要： There are provided a method and apparatus for detecting nucleic acid using bead and nanopore, and more specifically, a method and apparatus capable of detecting nucleic acid fragments of 70 bps to 300 bps in length by a nanopore detection unit with nanopores of 20 to 120 nm in diameter by attaching a bead to a nucleic acid probe and then detecting the bead attached to nucleic acid not nucleic acid itself. Accordingly, the present invention can detect the nucleic acid fragments using the nanopore detection unit with nanopores of 20 to 120 nm in diameter, even in case where Polymerase Chain Reaction (PCR) products are given as the sample, particularly the PCR products are the nucleic acid fragments of 70 to 300 bps in length.

    摘要翻译： 提供了使用珠粒和纳米孔检测核酸的方法和装置，更具体地，可以通过具有20至120nm的纳米孔的纳米孔检测单元来检测长度为70bps至300bps的核酸片段的方法和装置 通过将珠附着到核酸探针上，然后检测附着于核酸而不是核酸本身的珠。 因此，即使在给出聚合酶链反应（PCR）产物作为样品的情况下，本发明也可以使用直径为20-120nm的纳米孔的纳米孔检测单元来检测核酸片段，特别是PCR产物是核酸 长度为70至300 bps的酸性片段。

    Biochemical analyzer and method of controlling internal temperature of the biochemical analyzer
    3.
    发明授权
    Biochemical analyzer and method of controlling internal temperature of the biochemical analyzer 有权
    生化分析仪和生化分析仪内部温度控制方法

    公开(公告)号：US07943088B2

    公开(公告)日：2011-05-17

    申请号：US11972708

    申请日：2008-01-11

    申请人： Jeong-gun Lee ,  Yong-moo Shin ,  Kui-hyun Kim ,  Jin-han Chung

    发明人： Jeong-gun Lee ,  Yong-moo Shin ,  Kui-hyun Kim ,  Jin-han Chung

    IPC分类号： C12Q1/68 ,  G01N15/06

    CPC分类号： B01L7/52 ,  B01L3/5027 ,  B01L9/527 ,  B01L2300/0681 ,  B01L2300/0806 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1844 ,  B01L2300/1883 ,  B01L2400/0409 ,  G01N35/00069 ,  G01N2035/00346 ,  Y10T436/11 ,  Y10T436/25

    摘要： Provided is a biochemical analyzer in which a microfluidic biochemical assay may be performed. The analyzer includes: a microfluidic device loading space including a microfluidic device supporting unit detachably supporting a microfluidic device including an energy application region in which an energy is applied; an energy source loading space including an energy source applying the energy to the radiation application region; and an isolation wall isolating the microfluidic device loading space and the energy source loading space to prevent heat transfer between the microfluidic device loading space and the energy source loading space and including a window through which the energy can be transmitted. A method of controlling an internal temperature of the biochemical analyzer is also provided.

    摘要翻译： 提供了可以进行微流体生化测定的生化分析仪。 该分析仪包括：微流体装置装载空间，其包括微流体装置支撑单元，其可拆卸地支撑包括其中施加能量的能量施加区域的微流体装置; 能量源装载空间，其包括将能量施加到所述辐射施加区域的能量源; 隔离壁隔离微流体装置加载空间和能量源装载空间，以防止微流体装置加载空间与能量源装载空间之间的热传递并且包括能够传输能量的窗口。 还提供了一种控制生化分析仪内部温度的方法。

    Sensing switch and detecting method using the same
    4.
    发明授权
    Sensing switch and detecting method using the same 有权
    感应开关及使用其的检测方法

    公开(公告)号：US07790440B2

    公开(公告)日：2010-09-07

    申请号：US12272183

    申请日：2008-11-17

    申请人： Kyu-tae Yoo ,  Joon-ho Kim ,  Jun-hong Min ,  Sung-ouk Jung ,  Ji-na Namgoong ,  Kui-hyun Kim ,  Jeo-young Shim ,  Kak Namkoong

    发明人： Kyu-tae Yoo ,  Joon-ho Kim ,  Jun-hong Min ,  Sung-ouk Jung ,  Ji-na Namgoong ,  Kui-hyun Kim ,  Jeo-young Shim ,  Kak Namkoong

    IPC分类号： C12M3/00

    CPC分类号： G01N33/54373

    摘要： Provided are a sensing switch and a sensing method using the same. The sensing switch includes: a substrate; a supporter on the substrate; a sensing plate that is connected to a side of the supporter and is in parallel with the substrate by a predetermined distance; a receptor binding region on an upper surface of an end portion of the sensing plate; an electric or magnetic field generation device that induces deflection of the sensing plate when a receptor bound to the receptor binding region is selectively bound to an electrically or magnetically active ligand; and a pair of switching electrodes that are separated by a predetermined distance and is connected when the sensing plate contacts the substrate due to the deflection of the sensing plate. A target material need not be labelled, a signal processing of a fluorescent or electrical detection signal using an analysis apparatus is not required, and a signal can be directly decoded by confirming whether a current flows through the switch.

    摘要翻译： 提供了一种感测开关和使用该感测开关的感测方法。 感测开关包括：基板; 底物上的支撑物; 感测板，其与所述支撑体的一侧连接并且与所述基板平行预定距离; 在感测板的端部的上表面上的受体结合区域; 当与受体结合区结合的受体选择性地结合到电或磁性活性配体时，引起感测板偏转的电场或磁场产生装置; 以及一对分离预定距离的开关电极，并且当感测板由于感测板的偏转而与基板接触时被连接。 目标材料不需要标注，不需要使用分析装置的荧光或电检测信号的信号处理，并且可以通过确认电流是否流过开关直接解码信号。

    CELL CULTURE CHIP AND METHOD FOR REAL-TIME MONITORING OF A CELL CULTURE USING THE SAME
    7.
    发明申请
    CELL CULTURE CHIP AND METHOD FOR REAL-TIME MONITORING OF A CELL CULTURE USING THE SAME 审中-公开
    用于实时监测细胞培养物的细胞培养物芯片和方法

    公开(公告)号：US20070275435A1

    公开(公告)日：2007-11-29

    申请号：US11625847

    申请日：2007-01-23

    申请人： Kui-hyun KIM ,  Jung-im HAN ,  Jun-hong MIN ,  Kwang-ho CHEONG ,  Byung-chul KIM

    发明人： Kui-hyun KIM ,  Jung-im HAN ,  Jun-hong MIN ,  Kwang-ho CHEONG ,  Byung-chul KIM

    IPC分类号： C12Q1/02 ,  C12M1/00

    CPC分类号： C12M41/36 ,  C12M41/12 ,  C12M41/26 ,  C12M41/34

    摘要： Disclosed herein are a cell culture chip for monitoring a cell culture in real time and a method of monitoring the cell culture using the cell culture chip. The cell culture chip includes a cell culture chamber formed by side walls of a non-conductive material and a bottom layer of an insulating material and capable of accommodating a cell culture media. The cell culture chip also includes a semiconductor layer disposed under the bottom layer, a metal layer disposed under the semiconductor layer, and an electrode disposed in the cell culture chamber. The cell culture chip monitors both the states of cells attached to walls of the cell culture chamber and the states of cells floating in the cell culture media.

    摘要翻译： 本文公开了用于实时监测细胞培养物的细胞培养芯片和使用细胞培养芯片监测细胞培养的方法。 细胞培养芯片包括由非导电材料的侧壁和绝缘材料的底层形成并能够容纳细胞培养基的细胞培养室。 细胞培养芯片还包括设置在底层下方的半导体层，设置在半导体层下方的金属层和设置在细胞培养室中的电极。 细胞培养芯片监测附着于细胞培养室壁的细胞状态和悬浮在细胞培养基中的细胞状态。

    METHOD AND DEVICE FOR DETECTING DNA USING SURFACE-TREATED NANOPORE
    9.
    发明申请
    METHOD AND DEVICE FOR DETECTING DNA USING SURFACE-TREATED NANOPORE 审中-公开
    用于使用表面处理的纳米层检测DNA的方法和装置

    公开(公告)号：US20070218471A1

    公开(公告)日：2007-09-20

    申请号：US11550099

    申请日：2006-10-17

    申请人： Young Rok Kim ,  Jun Hong Min ,  In Ho Lee ,  Ah gi Kim ,  Kui Hyun Kim

    发明人： Young Rok Kim ,  Jun Hong Min ,  In Ho Lee ,  Ah gi Kim ,  Kui Hyun Kim

    IPC分类号： C12Q1/68 ,  C12M3/00

    CPC分类号： G01N33/48721 ,  C12Q1/6825 ,  C12Q2565/631

    摘要： Disclosed herein is a method for detecting DNA using a nanopore including treating the surface of a nanopore formed in a solid substrate with a substance carrying positive charges; introducing a DNA-containing sample into the surface-treated nanopore; and detecting electrical signals generated during translocation of the sample through the nanopore. Also disclosed herein is device for detecting DNA using a nanopore including a solid substrate including a nanopore, treated with a substance which carries positive charges to change a surface property of the nanopore so that the nanopore surface carries positive charges; an electrode applying voltage to the nanopore of the solid substrate; and a measurement unit measuring an electrical signal generated during translocation of a DNA-containing sample through the nanopore.

    摘要翻译： 本文公开了一种使用纳米孔检测DNA的方法，包括用携带正电荷的物质处理在固体基质中形成的纳米孔的表面; 将含DNA样品引入表面处理的纳米孔中; 并检测通过纳米孔在样品易位期间产生的电信号。 本文还公开了使用纳米孔检测DNA的装置，所述纳米孔包括包含纳米孔的固体基质，用携带正电荷的物质处理以改变纳米孔的表面性质，使得纳米孔表面带有正电荷; 电极向固体基底的纳米孔施加电压; 以及测量单元，其测量通过纳米孔在含DNA样品的易位期间产生的电信号。