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公开(公告)号：US20090325248A1
公开(公告)日：2009-12-31
申请号：US12067266
申请日：2006-10-09
摘要： The present invention relates to a cell which is genetically modified in relation to its wild type and which exhibits at least one of the properties a) or b): a) an increased activity by comparison with its wild type of an enzyme E1 which catalyzes the conversion of pyruvate into oxaloacetate, or of an enzyme E1b which catalyzes the conversion of phosphoenolpyruvate into oxaloacetate, b) an increased activity by comparison with its wild type of an enzyme E2 which catalyzes the conversion of aspartate into beta-alanine, where, besides properties a) or b), the cell is characterized by at least one of the properties c) or d) c) the genetically modified cell is able to export beta-alanine out of the cell, d) the genetically modified cell is able to convert beta-alanine into 3-hydroxypropionic acid. The invention also relates to methods for producing a genetically modified cell, to the genetically modified cells obtainable by this method, to methods for producing 3-hydroxypropionic acid, to a method for producing acrylic acid, to a method for producing polyacrylates, to a method for producing acrylic esters, and to the use of cells for producing 3-hydroxypropionic acid.
摘要翻译： 本发明涉及一种相对于其野生型进行遗传修饰的细胞，其表现出以下特征中的至少一种：a）或b）：a）通过与其野生型的酶E1相比增加活性，所述酶E1催化 或丙酮酸转化为草酰乙酸的酶E1b或催化磷酸烯醇丙酮酸转化为草酰乙酸的酶E1b; b）通过与其野生型的催化天冬氨酸转化成β-丙氨酸的酶E2的活性相比增加活性， a）或b），所述细胞的特征在于c）或d）中的至少一个c）所述遗传修饰的细胞能够将β-丙氨酸从细胞中出来，d）转基因细胞能够转化 β-丙氨酸转化为3-羟基丙酸。 本发明还涉及生产聚丙烯酸酯的方法，生产丙烯酸的方法，生产聚丙烯酸酯的方法，生产聚丙烯酸酯的方法，生产聚丙烯酸酯的方法，生产聚丙烯酸酯的方法 用于制备丙烯酸酯，以及用于生产3-羟基丙酸的细胞。
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公开(公告)号：US09234218B2
公开(公告)日：2016-01-12
申请号：US12602593
申请日：2008-05-30
申请人： Achim Marx , Markus Poetter , Stefan Buchholz , Alexander May , Hermann Siegert , Birgit Alber , Georg Fuchs , Lothar Eggeling
发明人： Achim Marx , Markus Poetter , Stefan Buchholz , Alexander May , Hermann Siegert , Birgit Alber , Georg Fuchs , Lothar Eggeling
IPC分类号： C12P7/42 , C12P7/52 , C12N9/02 , C12P7/40 , C12P7/62 , C08F120/06 , C08F120/10 , C12N9/10 , C12N9/16 , C12N9/88
CPC分类号： C12P7/42 , C08F120/06 , C08F120/10 , C12N9/0008 , C12N9/001 , C12N9/1096 , C12N9/16 , C12N9/88 , C12P7/40 , C12P7/52 , C12P7/625 , C12Y102/04004 , C12Y103/99012 , C12Y206/01042 , C12Y301/02004 , C12Y402/01017
摘要： The present invention relates to a process for the preparation of methacrylic acid or methacrylic esters, comprising the process steps of IA) preparation of 3-hydroxyisobutyric acid by a process comprising the process step of bringing a cell which has been genetically modified in comparison with its wild type in such a way that it is capable of forming more 3-hydroxyisobutyric acid, or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid in comparison with its wild type, into contact with a nutrient medium comprising, as carbon source, carbohydrates, glycerol, carbon dioxide, methanol, L-valine or L-glutamate under conditions under which 3-hydroxyisobutyric acid or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid are formed from the carbon source, if appropriate, isolation of the 3-hydroxyisobutyric acid from the nutrient medium and also, if appropriate, neutralization of the 3-hydroxyisobutyric acid, IB) dehydration of the 3-hydroxyisobutyric acid with formation of methacrylic acid and also, where appropriate, esterification methacrylic acid. The invention also relates to a process for the preparation of polymethacrylic acid or polymethacrylic esters.
摘要翻译： 本发明涉及一种制备甲基丙烯酸或甲基丙烯酸酯的方法，其包括以下工艺步骤：IA）通过包括以下步骤的方法制备3-羟基异丁酸，所述方法包括将经遗传修饰的细胞与其 野生型，使得其能够形成更多的3-羟基异丁酸或与其野生型相比基于3-羟基异丁酸的聚羟基链烷酸酯与营养培养基接触，所述营养培养基包含作为碳源的碳水化合物，甘油，碳 二氧化物，甲醇，L-缬氨酸或L-谷氨酸盐，其中3-羟基异丁酸或基于3-羟基异丁酸的聚羟基链烷酸酯由碳源形成的条件下，如果合适，则从营养培养基中分离出3-羟基异丁酸， 如果适当，中和3-羟基异丁酸，IB）3-羟基异丁酸脱水形成甲基丙烯酸 酸，并且在适当的情况下酯化甲基丙烯酸。 本发明还涉及一种制备聚甲基丙烯酸或聚甲基丙烯酸酯的方法。
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公开(公告)号：US20100291644A1
公开(公告)日：2010-11-18
申请号：US12602593
申请日：2008-05-30
申请人： Achim Marx , Markus Poetter , Stefan Buchholz , Alexander May , Hermann Siegert , Birgit Alber , Georg Fuchs , Lothar Eggeling
发明人： Achim Marx , Markus Poetter , Stefan Buchholz , Alexander May , Hermann Siegert , Birgit Alber , Georg Fuchs , Lothar Eggeling
CPC分类号： C12P7/42 , C08F120/06 , C08F120/10 , C12N9/0008 , C12N9/001 , C12N9/1096 , C12N9/16 , C12N9/88 , C12P7/40 , C12P7/52 , C12P7/625 , C12Y102/04004 , C12Y103/99012 , C12Y206/01042 , C12Y301/02004 , C12Y402/01017
摘要： The present invention relates to a process for the preparation of methacrylic acid or methacrylic esters, comprising the process steps of IA) preparation of 3-hydroxyisobutyric acid by a process comprising the process step of bringing a cell which has been genetically modified in comparison with its wild type in such a way that it is capable of forming more 3-hydroxyisobutyric acid, or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid in comparison with its wild type, into contact with a nutrient medium comprising, as carbon source, carbohydrates, glycerol, carbon dioxide, methanol, L-valine or L-glutamate under conditions under which 3-hydroxyisobutyric acid or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid are formed from the carbon source, if appropriate, isolation of the 3-hydroxyisobutyric acid from the nutrient medium and also, if appropriate, neutralization of the 3-hydroxyisobutyric acid, IB) dehydration of the 3-hydroxyisobutyric acid with formation of methacrylic acid and also, where appropriate, esterification methacrylic acid. The invention also relates to a process for the preparation of polymethacrylic acid or polymethacrylic esters.
摘要翻译： 本发明涉及一种制备甲基丙烯酸或甲基丙烯酸酯的方法，其包括以下工艺步骤：IA）通过包括以下步骤的方法制备3-羟基异丁酸，所述方法包括将经遗传修饰的细胞与其 野生型，使得其能够形成更多的3-羟基异丁酸或与其野生型相比基于3-羟基异丁酸的聚羟基链烷酸酯与营养培养基接触，所述营养培养基包含作为碳源的碳水化合物，甘油，碳 二氧化物，甲醇，L-缬氨酸或L-谷氨酸盐，其中3-羟基异丁酸或基于3-羟基异丁酸的聚羟基链烷酸酯由碳源形成的条件下，如果合适，则从营养培养基中分离出3-羟基异丁酸， 如果适当，中和3-羟基异丁酸，IB）3-羟基异丁酸脱水形成甲基丙烯酸 酸，并且在适当的情况下酯化甲基丙烯酸。 本发明还涉及一种制备聚甲基丙烯酸或聚甲基丙烯酸酯的方法。
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公开(公告)号：US07105321B2
公开(公告)日：2006-09-12
申请号：US10724827
申请日：2003-12-02
申请人： Bettina Moeckel , Caroline Kreutzer , Thomas Hermann , Mike Farwick , Achim Marx , Walter Pfefferle
发明人： Bettina Moeckel , Caroline Kreutzer , Thomas Hermann , Mike Farwick , Achim Marx , Walter Pfefferle
摘要： The invention relates to polynucleotides corresponding to the ccpA2 gene and which encode a CcpA2 catabolite control protein, methods of producing L-amino acids, and methods of screening for polynucleotides which encode proteins having CcpA2 catabolite control activity.
摘要翻译： 本发明涉及对应于ccpA2基因并且编码CcpA2分解代谢物控制蛋白质的多核苷酸，产生L-氨基酸的方法，以及筛选编码具有CcpA2分解代谢物控制活性的蛋白质的多核苷酸的方法。
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公开(公告)号：US06946271B2
公开(公告)日：2005-09-20
申请号：US09834722
申请日：2001-04-16
申请人： Mike Farwick , Klaus Huthmacher , Achim Marx , Walter Pfefferle
发明人： Mike Farwick , Klaus Huthmacher , Achim Marx , Walter Pfefferle
CPC分类号： C12N9/93 , C12P13/08 , C12Y602/01026
摘要： The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the menE gene is present in attenuated form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
摘要翻译： 本发明涉及一种分离的多核苷酸，其包含多核苷酸序列，所述多核苷酸序列选自a）与编码多肽的多核苷酸的程度相同的多核苷酸，所述多核苷酸包含SEQ ID NO： 2，b）编码多肽的多核苷酸，其包含与至少70％与SEQ ID No.2的氨基酸序列相同程度的氨基酸序列，c）多核苷酸，其与 ）或b），和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少所述menE 基因以减毒形式存在，并且使用包含根据本发明的序列的多核苷酸作为杂交探针。
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6.发明授权Nucleotide sequences encoding histidine kinase from corynebacterium glutamicum 失效编码来自谷氨酸棒杆菌的组氨酸激酶的核苷酸序列公开(公告)号：US06746854B2
公开(公告)日：2004-06-08
申请号：US09824551
申请日：2001-08-01
IPC分类号： C12P1308
CPC分类号： C12N9/1223 , C12P13/08
摘要： This invention relates to novel polynucleotide sequences encoding the histidine kinase luminescence expression sensor (luxS) gene from Corynebaclerium glutamicum, probes to the novel polynucleotide sequences encoding the luxS gene, vector and host cells containing the novel luxS polynucleotide sequences, the encoded Lux S polypeptide, and a process for the fermentative preparation of amino acids using bacteria which the luxS gene is attenuated.
摘要翻译： 本发明涉及编码来自谷氨酸棒杆菌的组氨酸激酶发光表达传感器（luxS）基因的新型多核苷酸序列，探针编码luxS基因的新型多核苷酸序列，含有新型luxS多核苷酸序列的载体和宿主细胞，编码的Lux S多肽， 以及使用luxS基因减毒的细菌发酵制备氨基酸的方法。
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公开(公告)号：US06632644B2
公开(公告)日：2003-10-14
申请号：US09731909
申请日：2000-12-08
申请人： Bettina Möckel , Walter Pfefferle , Achim Marx , Jörn Kalinowski , Brigitte Bathe , Alfred Pühler
发明人： Bettina Möckel , Walter Pfefferle , Achim Marx , Jörn Kalinowski , Brigitte Bathe , Alfred Pühler
IPC分类号： C12P1308
摘要： The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO 2 c) polynucleotide which is complementary to the polynucleotides of a) and b) and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acid with amplification of the zwa1 gene in the coryneform bacteria employed.
摘要翻译： 本发明涉及一种分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码多肽的多核苷酸的程度相同的多核苷酸，所述多核苷酸包含氨基酸序列 b）编码多肽的多核苷酸，其包含与SEQ ID NO 2的氨基酸序列至少70％的程度相同的氨基酸序列，c） 与a）和b）和d）多核苷酸互补的多核苷酸，其包含a），b）或c），/或PTEXT的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及发酵 在使用棒状细菌中扩增zwa1基因的L-氨基酸的制备
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公开(公告)号：US20110189742A1
公开(公告)日：2011-08-04
申请号：US13002519
申请日：2009-04-29
申请人： Thomas Haas , Thomas Tacke , Achim Marx , Alexander Schraven , Olivier Zehnacker , Eva Maria Wittmann
发明人： Thomas Haas , Thomas Tacke , Achim Marx , Alexander Schraven , Olivier Zehnacker , Eva Maria Wittmann
IPC分类号： C12P7/40
CPC分类号： C12P7/40 , C07C51/02 , C07C51/41 , C07C51/47 , C12P7/42 , C07C59/01 , C07C59/06 , C07C59/255 , C07C59/08 , C07C59/265
摘要： A process for the preparation of free carboxylic acids including: A) preparation of carboxylic acid by a biological cell located in an aqueous medium with addition of an amine of formula (I) where R1, R2 and R3, independently of one another, are identical or different, branched or unbranched, optionally substituted hydrocarbon radicals or H; B) for cases where the added amine A) is water-soluble, addition of a water-insoluble amine of formula (I), where, in A) or B), a multiphase system is obtained and the corresponding ammonium carboxylate is formed from the water-insoluble amine and the carboxylic acid; C) removal of the water-insoluble phase; and D) heating of the water-insoluble phase with release of free carboxylic acid.
摘要翻译： 一种制备游离羧酸的方法，包括：A）通过加入式（I）的胺的位于水性介质中的生物细胞制备羧酸，其中R1，R2和R3彼此独立地相同 或不同的支链或非支链的任选取代的烃基或H; B）对于添加的胺A）是水溶性的，加入式（I）的水不溶性胺，其中在A）或B）中获得多相体系，并且相应的羧酸铵由 水不溶性胺和羧酸; C）去除水不溶性相; 和D）释放游离羧酸时加热水不溶性相。
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公开(公告)号：US20100068773A1
公开(公告)日：2010-03-18
申请号：US12303161
申请日：2007-06-01
申请人： Achim Marx , Markus Poetter , Stefen Buchholz , Alexander May , Hermann Siegert , Georg Fuchs , Birgit Alber , Lothar Eggeling
发明人： Achim Marx , Markus Poetter , Stefen Buchholz , Alexander May , Hermann Siegert , Georg Fuchs , Birgit Alber , Lothar Eggeling
CPC分类号： C12P7/42 , C08F120/06 , C08F120/10 , C12N9/0008 , C12N9/001 , C12N9/1096 , C12N9/16 , C12N9/88 , C12P7/40 , C12P7/52 , C12P7/625 , C12Y102/04004 , C12Y103/99012 , C12Y206/01042 , C12Y301/02004 , C12Y402/01017
摘要： The present invention relates to a cell which has been modified in comparison with its wild type in such a way that it is capable of forming more, by comparison with its wild, 3-hydroxyisobutyric acid or poly-hydroxyalkanoates based on 3-hydroxyisobutyric acid via methylmalonate-semialdehyde or 3-hydroxybutyryl-coenzyme A as precursors. The invention also relates to a method of generating a genetically modified cell, to the genetically modified cell obtainable by these methods, to a method of producing 3-hydroxyisobutyric acid or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid, to a method of producing methacrylic acid or methacrylic esters, and to a method of producing polymethacrylic acid or polymethacrylic esters. The present invention furthermore relates to an isolated DNA, to a vector, to the use of this vector for transforming a cell, to a transformed cell, and to a polypeptide.
摘要翻译： 本发明涉及与其野生型相比被修饰的细胞，使得其能与其野生型3-羟基异丁酸或基于3-羟基异丁酸的聚羟基链烷酸相比形成更多的细胞 甲基丙二酸酯 - 半醛或3-羟基丁酰辅酶A作为前体。 本发明还涉及生产基于3-羟基异丁酸的3-羟基异丁酸或聚羟基链烷酸酯的生产方法，该方法可以通过这些方法产生遗传修饰的细胞，生产甲基丙烯酸或其制备方法 甲基丙烯酸酯，以及生产聚甲基丙烯酸或聚甲基丙烯酸酯的方法。 本发明还涉及分离的DNA，载体，使用该载体转化细胞，转化细胞和多肽。
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公开(公告)号：US07262036B2
公开(公告)日：2007-08-28
申请号：US11419529
申请日：2006-05-22
申请人： Bettina Hädrich , Walter Pfefferle , Achim Marx , Jörn Kalinowski , Brigitte Bathe , Alfred Pühler
发明人： Bettina Hädrich , Walter Pfefferle , Achim Marx , Jörn Kalinowski , Brigitte Bathe , Alfred Pühler
摘要： Disclosed herein is an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO:2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO:2 c) polynucleotide which is complementary to the polynucleotides of a) and b) and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acid with amplification of the zwa1 gene in the coryneform bacteria employed.
摘要翻译： 本文公开了一种分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码包含SEQ ID NO：2的氨基酸序列的多肽的多核苷酸的程度相同的多核苷酸 b）编码多肽的多核苷酸，其包含与SEQ ID NO：2的氨基酸序列至少70％相同程度的氨基酸序列c）与a）和 b）和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及在所用棒状细菌中扩增zwa1基因的L-氨基酸的发酵制剂的方法。
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