Method and Device for Collecting Suspended Particles
    2.
    发明申请
    Method and Device for Collecting Suspended Particles 失效
    收集悬浮颗粒的方法和装置

    公开(公告)号:US20070221501A1

    公开(公告)日:2007-09-27

    申请号:US11568895

    申请日:2005-05-06

    IPC分类号: B01D57/02

    CPC分类号: B03C5/026

    摘要: A description is given of methods for collecting particles (1, 2) which are suspended in a liquid, including the following steps: providing the liquid containing the suspended particles (1, 2) in a compartment (10) having lateral surfaces (11), wherein at least one electrode (21) is arranged on at least one of the lateral surfaces (11), and generating high-frequency electric fields by means of the at least one electrode (21) so as to form at least one circulating flow (30), by means of which the particles (1, 2) are guided to at least one predetermined collecting area (40) in the compartment (10), wherein the flow (30) is formed in such a way that at least one branch of the flow runs along a longitudinal extent of the at least one electrode (21), and the flow (30) circulates about an axis (31) which is oriented perpendicular to the respectively adjacent lateral surface (11) with the electrode (21). Corresponding devices for collecting particles are also described.

    摘要翻译: 给出了用于收集悬浮在液体中的颗粒(1,2)的方法的描述,包括以下步骤:将含有悬浮颗粒(1,2)的液体提供在具有侧表面(11)的隔室(10)中, ,其中至少一个电极(21)布置在所述侧表面(11)中的至少一个上,并且通过所述至少一个电极(21)产生高频电场,以便形成至少一个循环流 (30),所述颗粒(1,2)通过所述颗粒(1,2)被引导到所述隔室(10)中的至少一个预定收集区域(40),其中所述流动(30)形成为使得至少一个 流动的分支沿着至少一个电极(21)的纵向延伸,并且流动(30)围绕垂直于相邻的侧表面(11)的电极(21)的轴线(31)循环 )。 还描述了用于收集颗粒的相应装置。

    Docking device for a fluidic microsystem
    4.
    发明申请
    Docking device for a fluidic microsystem 审中-公开
    流体微系统的对接装置

    公开(公告)号:US20060198766A1

    公开(公告)日:2006-09-07

    申请号:US10556095

    申请日:2004-05-10

    IPC分类号: B01L9/00

    摘要: The invention relates to a docking device (100) for retaining a fluidic microsystem (10), comprising a base plate (20) and a retaining frame (30), movable relative to the base plate (20) and used to fasten the microsystem (10) on the base plate (20). The retaining frame (30) is adapted to detachably receive the microsystem (10) and can be detached from the base plate (20) together with the microsystem (10). The device is also provided with a clamping device (40) with which the microsystem (10) on the retaining frame (30) can be pushed against the base plate (20). The invention also relates to methods for operating a fluidic microsystem (10) using said docking device (100).

    摘要翻译: 本发明涉及一种用于保持流体微系统(10)的对接装置(100),其包括可相对于基板(20)移动并用于紧固微系统(20)的基板(20)和保持框架(30) 10)在基板(20)上。 保持框架(30)适于可拆卸地接收微系统(10)并且可以与微系统(10)一起从基板(20)拆下。 该装置还设置有夹紧装置(40),保持框架(30)上的微系统(10)可以通过该夹紧装置推靠在基板(20)上。 本发明还涉及使用所述对接装置(100)操作流体微系统(10)的方法。

    Cryopreserving and cryogenically processing biological objects
    6.
    发明授权
    Cryopreserving and cryogenically processing biological objects 失效
    冷冻保存和低温处理生物体

    公开(公告)号:US5925511A

    公开(公告)日:1999-07-20

    申请号:US836092

    申请日:1997-08-29

    IPC分类号: A01N1/00 A01N1/02 A01N3/00

    摘要: For the fixated cryopreservation of single living biological objects or s objects compiled in a given number (for example cells) a cryogenically cooled substrate (13) is jetted therewith in a enveloping solution in microdroplet form (12) from a storage vessel, for example by means of a microdroplet jetting device (11). The substrate is temperature-controlled via a coolant (15), the surface to be jetted being located in a gas atmosphere or in vacuum (14). The substrate surface is maintained at a temperature T1 resulting in freezing of the impinging microdroplet, the substrate surface being possibly supportingly microstructured and comprising sensing elements. By controlled movement of either the substrate or the microdroplet jetting device the microdroplets can be applied singly and in patterns in arrays freely selectable or predetermined by the structuring of the substrate. The substrate with the applied microdroplets and the cells frozen therein are stored and/or processed by a processing means (17) ablating the material or applying said material at temperatures T2 of down to -273.degree. C. In the cryogenic condition manipulations such as removal by mechanical ablation or addition of solutions or substances to the surface of the substrate can be undertaken. During thawing the substrate surface is brought to a temperature T1 above the freezing point of the enveloping solution and the microdroplet array thawed in a predefined manner.

    摘要翻译: PCT No.PCT / DE95 / 01490 Sec。 371日期1997年8月29日 102(e)日期1997年8月29日PCT提交1995年10月26日PCT公布。 第WO96 / 13159号公报 日期1996年5月9日对于以一定数量(例如细胞)编制的单一活生物体或此类物体的固定式低温保存,将低温冷却的基底(13)以微滴形式(12)的包封溶液从储存容器 ,例如通过微滴喷射装置(11)。 衬底通过冷却剂(15)进行温度控制,待喷射的表面位于气体气氛或真空中(14)。 衬底表面保持在温度T1,导致撞击微滴的冻结,衬底表面可能被支撑地微结构化并且包括感测元件。 通过基板或微滴喷射装置的受控运动,微滴可以单独施加,并且可以通过基板的结构自由选择或预定的阵列中的图案施加。 具有施加的微滴和在其中冷冻的细胞的基质通过处理装置(17)储存和/或处理,该处理装置(17)在低至-273℃的温度T2下烧蚀材料或施加所述材料。在低温条件下操作,例如去除 可以通过机械消融或将溶液或物质添加到基材表面进行。 在解冻期间,将衬底表面的温度T1提高到高于包络溶液的凝固点并且微滴阵列以预定方式解冻。

    Method and Device for Handling Sedimenting Particles
    7.
    发明申请
    Method and Device for Handling Sedimenting Particles 审中-公开
    处理沉淀颗粒的方法和装置

    公开(公告)号:US20080296157A1

    公开(公告)日:2008-12-04

    申请号:US12088009

    申请日:2006-09-27

    摘要: The invention relates to a method for handling particles (1, 2) that are suspended in a carrier liquid (3). The method includes the following steps: the carrier liquid (3) is received with the particles (1, 2) in a liquid siphoning device (10) including at least one siphoning opening (11), electrical and/or magnetic separating fields are generated in the liquid siphoning device (10), a sedimentation movement of the particles (1, 2) is created in the liquid, each particle having a sedimentation speed that depends on the action of the separating fields on the particle (1, 2) and the particles (1, 2) forming a plurality of particle fractions (5, 6) according to the sedimentation speeds thereof, and the particle fractions (5, 6) are separately extracted from the liquid siphoning device (10). The invention also relates to a handling device (100) for handling suspended particles (1, 2).

    摘要翻译: 本发明涉及一种处理悬浮在载体液体(3)中的颗粒(1,2)的方法。 该方法包括以下步骤:载体液体(3)在包括至少一个虹吸孔(11)的液体虹吸装置(10)中与颗粒(1,2)一起接收,产生电和/或磁分离场 在液体虹吸装置(10)中,在液体中产生颗粒(1,2)的沉降运动,每个颗粒的沉降速度取决于颗粒(1,2)上的分离场的作用和 从液体虹吸装置(10)分别提取根据其沉降速度形成多个颗粒部分(5,6)和颗粒部分(5,6)的颗粒(1,2)。 本发明还涉及一种用于处理悬浮颗粒(1,2)的处理装置(100)。

    Microfluidic system and associated operational method
    9.
    发明申请
    Microfluidic system and associated operational method 审中-公开
    微流控系统及相关操作方法

    公开(公告)号:US20070151855A1

    公开(公告)日:2007-07-05

    申请号:US10597696

    申请日:2005-02-03

    IPC分类号: B03C5/02

    摘要: The invention relates to a microfluidic system, in particular in a cell sorter, including a first carrier power supply (1) which is used to supply a first carrier flow having particles (4) suspended therein, a first carrier power output line (15) which is used to withdraw at least one part of the carrier flow having particles (4) suspended therein, a process chamber (3) which is used to examine, observe, manipulate and/or select the particle (4). The first carrier power supply (1) flows into the process chamber (3) when the first carrier power output line (15) is discharged from the process chamber (3). According to the invention, at least one second carrier power supply (2) flows into the process chamber (3) in order to supply a second carrier flow having particles (5) suspended therein. The invention also relates to a corresponding operational method.

    摘要翻译: 本发明涉及微流体系统,特别是在细胞分选机中,包括用于提供悬浮在其中的颗粒(4)的第一载体流的第一载体电源(1),第一载体功率输出线(15) 其用于抽出具有悬浮在其中的颗粒(4)的载体流的至少一部分,用于检查,观察,操纵和/或选择颗粒(4)的处理室(3)。 当第一载体动力输出管线(15)从处理室(3)排出时,第一载体电源(1)流入处理室(3)。 根据本发明,至少一个第二载体电源(2)流入处理室(3),以便提供具有悬浮在其中的颗粒(5)的第二载体流。 本发明还涉及相应的操作方法。

    Sample depositing device for a cell sorter
    10.
    发明申请
    Sample depositing device for a cell sorter 审中-公开
    细胞分选机的样品沉积装置

    公开(公告)号:US20070111298A1

    公开(公告)日:2007-05-17

    申请号:US10556140

    申请日:2004-05-10

    IPC分类号: C12M3/00

    摘要: The invention relates to a sample depositing device, particularly for a cell sorter, comprising a sample supply (38, 39) for supplying samples to be deposited and comprising a sample storage element (36) for storing the samples. Said sample storage element (36) has a number of sample containers for storing the samples separately. The invention provides that the sample storage element (36) is mounted in a displaceable manner in order to select a sample container whereas the sample supply (38, 39) is stationary.

    摘要翻译: 本发明涉及一种样品沉积装置,特别是用于细胞分选机的样品沉积装置,其包括用于提供待沉积样品的样品供应源(38,39),并包括用于储存样品的样品存储元件(36)。 所述样品存储元件(36)具有用于分别存储样品的多个样品容器。 本发明提供了样品存储元件(36)以可移位的方式安装,以便选择样品容器,而样品供应(38,39)是静止的。