公开(公告)号：US20050255445A1

公开(公告)日：2005-11-17

申请号：US10516473

申请日：2003-06-03

申请人： Hendrik Van Damme ,  Herman Blok ,  Maria Helena Hilhorst ,  Colin Ingham

发明人： Hendrik Van Damme ,  Herman Blok ,  Maria Helena Hilhorst ,  Colin Ingham

IPC分类号： B01J19/00 ,  C12M1/00 ,  C12M1/34 ,  C12N15/09 ,  C12Q1/02 ,  C12Q1/68 ,  C40B30/04 ,  C40B60/14 ,  G01N33/15 ,  G01N33/50 ,  G01N33/551 ,  G01N33/569 ,  G01N33/68 ,  G01N37/00 ,  C12Q1/00

CPC分类号： C40B30/04 ,  B01J2219/00286 ,  B01J2219/00378 ,  B01J2219/00527 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01J2219/00743 ,  C40B60/14 ,  G01N33/5008 ,  G01N33/5023 ,  G01N33/5026 ,  G01N33/551 ,  G01N33/56911 ,  G01N33/6854 ,  G01N2500/20

摘要： The present invention relates to methods for screening of cellular responses of cellular components comprising: (a) providing cellular components on the surface of a substrate, said substrate having immobilized thereon an array of detector molecules; (b) delivering test compounds to positions on the substrate corresponding to the arrayed detector molecules on the surface of said solid substrate; (c) incubating said test compounds with said cellular components on the surface of the solid support, under conditions allowing the induction of cellular responses; (d) assaying said cellular responses; and, identifying and characterizing the cellular responses induced by said test compounds. The present invention further relates to the uses of said methods as well as microarrays and kits for carrying out said methods.

摘要翻译： 本发明涉及筛选细胞成分的细胞反应的方法，包括：（a）在基质的表面上提供细胞组分，所述底物固定有检测器分子阵列; （b）将测试化合物输送到与所述固体基底表面上的阵列检测器分子相对应的基底上的位置; （c）在允许诱导细胞反应的条件下，将所述测试化合物与固体支持物表面上的所述细胞组分一起孵育; （d）测定所述细胞反应; 以及鉴定和表征由所述测试化合物诱导的细胞应答。 本发明还涉及所述方法的用途以及用于实施所述方法的微阵列和试剂盒。

公开(公告)号：US20070072187A1

公开(公告)日：2007-03-29

申请号：US10579896

申请日：2004-11-29

申请人： Herman Blok ,  Hendrik Van Damme ,  Colin Ingham ,  Maria Hilhorst

发明人： Herman Blok ,  Hendrik Van Damme ,  Colin Ingham ,  Maria Hilhorst

IPC分类号： C12Q1/68 ,  G01N33/567 ,  C12M3/00 ,  C40B30/06 ,  C40B40/10

CPC分类号： G01N33/5035 ,  B01J2219/00364 ,  B01J2219/00369 ,  B01J2219/00378 ,  B01J2219/00387 ,  B01J2219/005 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00641 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/00691 ,  B01J2219/00743 ,  B01L3/50255 ,  B01L3/5085 ,  B01L9/52 ,  B01L2300/0636 ,  B01L2300/0887 ,  G01N33/5008 ,  G01N33/5026 ,  G01N33/5076 ,  G01N33/5085 ,  G01N33/5088 ,  G01N2035/00158

摘要： The present invention relates methods for screening of cellular responses comprising: (a) providing a solid porous support having first and second surfaces and at least one area with a plurality of through-going channels (b) providing cellular components on said first and/or second surface of said solid porous support, wherein said solid porous support retains said cellular compounds on its surface; (c) providing a supply chamber at said first and/or second surface and opposite to said cellular components (d) subjecting all or part of said cellular components to one or more effectors; wherein at least one effector is delivered from said supply chamber through the porous support; (e) incubating the said all or part of cellular components with said effectors under conditions allowing the induction of cellular responses in the said all or part of cellular components; (f) optionally providing detector molecules to the said all or part of cellular components for assaying cellular responses (g) assaying for cellular responses; and, (h) identifying and characterizing the cellular responses induced by said effector molecules. The present invention further relates to the uses of said methods and apparatuses for carrying out said methods as well as to the use of a porous support for the preparation of a microarray kit for carrying out said methods.

摘要翻译： 本发明涉及用于筛选细胞反应的方法，其包括：（a）提供具有第一和第二表面的固体多孔载体和至少一个具有多个通过通道的区域（b）在所述第一和/或第二表面上提供细胞组分， 所述固体多孔载体的第二表面，其中所述固体多孔载体在其表面上保留所述多孔化合物; （c）在所述第一和/或第二表面处提供与所述细胞组分相反的供应室（d），使所有或部分所述细胞组分经受一个或多个效应物; 其中至少一个效应器通过所述多孔载体从所述供应室输送; （e）在允许在所述全部或部分细胞组分中诱导细胞反应的条件下，将所述全部或部分细胞组分与所述效应子一起孵育; （f）任选地向所述全部或部分细胞组分提供检测器分子以测定细胞应答（g）细胞反应的测定; 和（h）鉴定和表征由所述效应分子诱导的细胞应答。 本发明还涉及用于实施所述方法的所述方法和装置的用途以及使用多孔载体来制备用于实施所述方法的微阵列试剂盒。

公开(公告)号：US08288521B2

公开(公告)日：2012-10-16

申请号：US13072442

申请日：2011-03-25

申请人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

发明人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

IPC分类号： C07H21/04 ,  C12N15/11

CPC分类号： C12Q1/6827 ,  B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00536 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00729 ,  B82Y30/00 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6876 ,  C12Q2600/156 ,  C40B40/06 ,  C40B60/14 ,  Y02A50/58 ,  C12Q2565/501 ,  C12Q2561/125 ,  C12Q2537/143 ,  C12Q2565/514

摘要： The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support.

摘要翻译： 本发明描述了用于鉴定多个靶核苷酸序列中一个或多个单碱基变化，插入，缺失或易位不同的多个序列中的一个或多个的方法。 该方法包括连接阶段，捕获阶段和检测阶段。 连接阶段利用具有目标序列特异性部分的一个寡核苷酸探针与可寻址阵列特异性部分之间的连接检测反应和具有靶序列特异性部分和可检测标记的第二寡核苷酸探针。 在连接阶段之后，捕获阶段通过将连接的寡核苷酸探针与具有固定化捕获寡核苷酸阵列的固体支持物杂交进行，其中至少一些与可寻址阵列特异性部分互补。 捕获阶段完成后，进行检测阶段以检测与固体支持物杂交的连接的寡核苷酸探针的标记。

公开(公告)号：US07893233B2

公开(公告)日：2011-02-22

申请号：US12725230

申请日：2010-03-16

申请人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

发明人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

IPC分类号： C07H21/04 ,  C12N15/11

CPC分类号： C12Q1/6827 ,  B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00536 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00729 ,  B82Y30/00 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6876 ,  C12Q2600/156 ,  C40B40/06 ,  C40B60/14 ,  Y02A50/58 ,  C12Q2565/501 ,  C12Q2561/125 ,  C12Q2537/143 ,  C12Q2565/514

摘要： The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support.

摘要翻译： 本发明描述了用于鉴定多个靶核苷酸序列中一个或多个单碱基变化，插入，缺失或易位不同的多个序列中的一个或多个的方法。 该方法包括连接阶段，捕获阶段和检测阶段。 连接阶段利用具有目标序列特异性部分的一个寡核苷酸探针与可寻址阵列特异性部分之间的连接检测反应和具有靶序列特异性部分和可检测标记的第二寡核苷酸探针。 在连接阶段之后，捕获阶段通过将连接的寡核苷酸探针与具有固定化捕获寡核苷酸阵列的固体支持物杂交进行，其中至少一些与可寻址阵列特异性部分互补。 捕获阶段完成后，进行检测阶段以检测与固体支持物杂交的连接的寡核苷酸探针的标记。

公开(公告)号：US07888009B2

公开(公告)日：2011-02-15

申请号：US10854482

申请日：2004-05-25

申请人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

发明人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6827 ,  B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00536 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00729 ,  B82Y30/00 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6876 ,  C12Q2600/156 ,  C40B40/06 ,  C40B60/14 ,  Y02A50/58 ,  C12Q2565/501 ,  C12Q2561/125 ,  C12Q2537/143 ,  C12Q2565/514

摘要： The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support.

公开(公告)号：US07083917B2

公开(公告)日：2006-08-01

申请号：US09963920

申请日：2001-09-26

申请人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

发明人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

IPC分类号： C12Q1/68 ,  C07H21/02 ,  G01N33/543

CPC分类号： C12Q1/6827 ,  B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00536 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00729 ,  B82Y30/00 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6876 ,  C12Q2600/156 ,  C40B40/06 ,  C40B60/14 ,  Y02A50/58 ,  C12Q2565/501 ,  C12Q2561/125 ,  C12Q2537/143 ,  C12Q2565/514

摘要： The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support. The ligation phase can be preceded by an amplification process. The present invention also relates to a kit for practicing this method, a method of forming arrays on solid supports, and the supports themselves.

公开(公告)号：US20060057032A1

公开(公告)日：2006-03-16

申请号：US10511922

申请日：2003-04-17

申请人： Herman Blok ,  Marinus Gerardus Van Beuningen

发明人： Herman Blok ,  Marinus Gerardus Van Beuningen

IPC分类号： B01L9/00

CPC分类号： B29C65/64 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/00495 ,  B01J2219/00641 ,  B01J2219/00662 ,  B01L3/50255 ,  B01L3/50851 ,  B01L7/00 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2300/042 ,  B01L2300/0636 ,  B01L2300/0829 ,  B01L2400/0487 ,  B01L2400/049 ,  B29C65/02 ,  B29C65/18 ,  B29C65/242 ,  B29C65/245 ,  B29C65/44 ,  B29C65/48 ,  B29C65/7847 ,  B29C66/003 ,  B29C66/1122 ,  B29C66/21 ,  B29C66/24221 ,  B29C66/30326 ,  B29C66/472 ,  B29C66/542 ,  B29C66/63 ,  B29C66/71 ,  B29C66/73365 ,  B29C66/742 ,  B29C66/7461 ,  B29C66/81433 ,  B29C66/8181 ,  B29C66/8282 ,  B29C66/8322 ,  B29C66/8432 ,  B29C66/92445 ,  B29C66/9292 ,  B29C66/92921 ,  B29K2023/00 ,  B29K2709/02 ,  B29L2031/756 ,  C40B60/14 ,  B29K2023/38

摘要： A substrate plate comprises a microplate (1) made of a plastic material, having an array of wells (2) arranged in rows and columns, the bottom of at least one well (2) being provided by a porous substrate (3). Each porous substrate (3) is incorporated into the well (2) by means of a thermal bond.

摘要翻译： 衬底板包括由塑料材料制成的微板（1），具有以行和列排列的孔阵列（2），至少一个孔（2）的底部由多孔基底（3）提供。 每个多孔基材（3）通过热粘合结合到孔（2）中。

公开(公告)号：US08624016B2

公开(公告)日：2014-01-07

申请号：US13492999

申请日：2012-06-11

申请人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

发明人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

IPC分类号： C07H21/04 ,  C12N15/11

CPC分类号： C12Q1/6827 ,  B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00536 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00729 ,  B82Y30/00 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6876 ,  C12Q2600/156 ,  C40B40/06 ,  C40B60/14 ,  Y02A50/58 ,  C12Q2565/501 ,  C12Q2561/125 ,  C12Q2537/143 ,  C12Q2565/514

摘要： The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support.

摘要翻译： 本发明描述了用于鉴定多个靶核苷酸序列中一个或多个单碱基变化，插入，缺失或易位不同的多个序列中的一个或多个的方法。 该方法包括连接阶段，捕获阶段和检测阶段。 连接阶段利用具有目标序列特异性部分的一个寡核苷酸探针与可寻址阵列特异性部分之间的连接检测反应和具有靶序列特异性部分和可检测标记的第二寡核苷酸探针。 在连接阶段之后，捕获阶段通过将连接的寡核苷酸探针与具有固定化捕获寡核苷酸阵列的固体支持物杂交进行，其中至少一些与可寻址阵列特异性部分互补。 捕获阶段完成后，进行检测阶段以检测与固体支持物杂交的连接的寡核苷酸探针的标记。

公开(公告)号：US08168129B2

公开(公告)日：2012-05-01

申请号：US11913034

申请日：2006-05-02

申请人： Dirk Vangeneugden ,  Robby Jozef Martin Rego ,  Danny Havermans ,  Herman Blok

发明人： Dirk Vangeneugden ,  Robby Jozef Martin Rego ,  Danny Havermans ,  Herman Blok

IPC分类号： A61L2/00 ,  B01J19/08 ,  H05F3/00 ,  H01J27/00

CPC分类号： A61L9/16 ,  A61L2/10 ,  A61L2/14 ,  A61L9/22 ,  C02F1/325 ,  C02F1/46109 ,  C02F1/48 ,  C02F1/725 ,  C02F2001/46152 ,  C02F2103/42 ,  C02F2305/10

摘要： An apparatus and method are for disinfection and purification of a liquid, gaseous or solid phase, or a mixture thereof. The apparatus includes: a central electrode, a dielectric layer adjacent to the electrode, a first area adjacent to the dielectric layer, and is configured to introduce a first medium into the first area, a second area adjacent to the first area. The apparatus is also configured to introduce a second medium into the second area, and for creating a plasma in the first medium, while the first medium is present in the first area, by applying a voltage between the first electrode and a second electrode. An injector injects the plasma into the second area, in order to be mixed with the second medium.

摘要翻译： 一种用于消毒和净化液体，气态或固相或其混合物的装置和方法。 该装置包括：中心电极，与电极相邻的电介质层，与电介质层相邻的第一区域，并且被配置为将第一介质引入第一区域，与第一区域相邻的第二区域。 该装置还被配置为通过在第一电极和第二电极之间施加电压，将第二介质引入第二区域，并且用于在第一介质中产生等离子体，同时第一介质存在于第一区域中。 喷射器将等离子体注入第二区域，以便与第二介质混合。

公开(公告)号：US07879579B2

公开(公告)日：2011-02-01

申请号：US09963698

申请日：2001-09-26

申请人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

发明人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

IPC分类号： C12P19/34

CPC分类号： C12Q1/6827 ,  B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00536 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00729 ,  B82Y30/00 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6876 ,  C12Q2600/156 ,  C40B40/06 ,  C40B60/14 ,  Y02A50/58 ,  C12Q2565/501 ,  C12Q2561/125 ,  C12Q2537/143 ,  C12Q2565/514

摘要： The present invention relates to a method of forming arrays of oligonucleotides on a solid support. This method involves providing a solid support having an array of positions each suitable for attachment of an oligonucleotide. Linkers, suitable for coupling oligonucleotides to the solid support, are attached to the solid support surface at each of the array positions. An array of a plurality of capture oligonucleotides are formed on the solid support by a series of cycles of activating selected array positions for attachment of multimer nucleotides and attaching multimer nucleotides at activated array positions. The multimer nucleotides are selected for attachment so that the capture oligonucleotides formed on the array hybridize with complementary oligonucleotide target sequences under uniform hybridization conditions.

摘要翻译： 本发明涉及在固体支持物上形成寡核苷酸阵列的方法。 该方法包括提供具有各自适合于连接寡核苷酸的位置阵列的固体支持物。 适用于将寡核苷酸与固体支持物偶联的接头在每个阵列位置处连接到固体支持物表面。 通过一系列激活所选阵列位置的循环在固体支持物上形成多个捕获寡核苷酸的阵列，用于连接多聚核苷酸并在激活的阵列位置附着多核苷酸。 选择多聚核苷酸进行连接，使得阵列上形成的捕获寡核苷酸在均匀杂交条件下与互补寡核苷酸靶序列杂交。