公开(公告)号：US06326083B1

公开(公告)日：2001-12-04

申请号：US09264519

申请日：1999-03-08

Applicant: Hua Yang ,  Steven A. Sundberg

Inventor： Hua Yang ,  Steven A. Sundberg

IPC: B32B1706

Abstract: Hydrophilic protein adsorption resistant coatings for microfluidic devices are provided. Additionally, microfluidic devices and methods of manufacturing microfluidic devices that include the coatings are provided.

Abstract translation: 提供了用于微流体装置的亲水性蛋白质吸附抗性涂层。 另外，提供了包括涂层的微流体装置和制造微流体装置的方法。

公开(公告)号：US06509059B2

公开(公告)日：2003-01-21

申请号：US09963925

申请日：2001-09-25

Applicant: Hua Yang ,  Steven A. Sundberg

Inventor： Hua Yang ,  Steven A. Sundberg

IPC: B05D722

CPC classification number: A61L31/10 ,  Y10T428/24562 ,  Y10T428/24661 ,  Y10T428/24744 ,  Y10T428/261 ,  Y10T428/31612 ,  Y10T428/31663 ,  C08L79/02

Abstract: Hydrophilic protein adsorption resistant coatings for microfluidic devices are provided. Additionally, microfluidic devices and methods of manufacturing microfluidic devices that include the coatings are provided.

公开(公告)号：US06841193B1

公开(公告)日：2005-01-11

申请号：US10651189

申请日：2003-08-28

Applicant: Hua Yang ,  Steven A. Sundberg

Inventor： Hua Yang ,  Steven A. Sundberg

IPC: A61L31/10 ,  B05D7/22

CPC classification number: A61L31/10 ,  Y10T428/24562 ,  Y10T428/24661 ,  Y10T428/24744 ,  Y10T428/261 ,  Y10T428/31612 ,  Y10T428/31663 ,  C08L79/02

Abstract: Methods of coating the surfaces of the microchannels of a microfluidic device are provided. These methods include silylating a first portion of the surfaces of the microchannels with a first silylating reagent to produce a first silylated surface, and silylating a second portion of the surfaces of the microchannels with a second silylating reagent to produce a second silylated surface.

Abstract translation: 提供了涂覆微流体装置的微通道表面的方法。 这些方法包括用第一甲硅烷基化试剂甲硅烷基化微通道表面的第一部分以产生第一甲硅烷基化表面，并用第二甲硅烷基化试剂甲硅烷基化第二部分微通道表面以产生第二甲硅烷基化表面。

公开(公告)号：US06660367B1

公开(公告)日：2003-12-09

申请号：US10279670

申请日：2002-10-24

Applicant: Hua Yang ,  Steven A. Sundberg

Inventor： Hua Yang ,  Steven A. Sundberg

IPC: B32B322

CPC classification number: A61L31/10 ,  Y10T428/24562 ,  Y10T428/24661 ,  Y10T428/24744 ,  Y10T428/261 ,  Y10T428/31612 ,  Y10T428/31663 ,  C08L79/02

Abstract: Hydrophilic protein adsorption resistant coatings for microfluidic devices are provided. Additionally, microfluidic devices and methods of manufacturing microfluidic devices that include the coatings are provided.

公开(公告)号：US5919523A

公开(公告)日：1999-07-06

申请号：US956986

申请日：1997-10-24

Applicant: Steven A. Sundberg ,  Jeffrey W. Jacobs ,  John R. Schullek ,  Glenn H. McGall ,  Andrei Mirzabekov ,  Edward Timofeey ,  David Fujimoto ,  Christopher P. Holmes ,  Hua Yang

Inventor： Steven A. Sundberg ,  Jeffrey W. Jacobs ,  John R. Schullek ,  Glenn H. McGall ,  Andrei Mirzabekov ,  Edward Timofeey ,  David Fujimoto ,  Christopher P. Holmes ,  Hua Yang

IPC: B01J19/00 ,  B05D1/18 ,  C03C17/30 ,  C03C17/34 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N33/543 ,  B05D1/36 ,  B05D3/02

CPC classification number: B82Y30/00 ,  B01J19/0046 ,  B05D1/185 ,  B82Y40/00 ,  C03C17/30 ,  C03C17/3405 ,  C40B80/00 ,  G01N33/54353 ,  B01J2219/00364 ,  B01J2219/00378 ,  B01J2219/0043 ,  B01J2219/005 ,  B01J2219/00504 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/0059 ,  B01J2219/00592 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00619 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01J2219/00725 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14

Abstract: Methods and derivatized supports which are useful in solid-phase synthesis of peptides, oligonucleotides or other small organic molecules as well as arrays of ligands. The methods provide means to control the functional site density on a solid support. Some of the derivatized supports are polymer-coated or glycan-coated. Other methods for regenerating the surface of a used ligand array are also provided.

Abstract translation: 可用于肽，寡核苷酸或其他小有机分子的固相合成以及配体阵列的方法和衍生的载体。 该方法提供了控制固体支持物上的功能位点密度的方法。 一些衍生的载体是聚合物涂覆的或聚糖包被的。 还提供了用于再生所用配体阵列的表面的其它方法。

公开(公告)号：US07364704B2

公开(公告)日：2008-04-29

申请号：US11493997

申请日：2006-07-27

Applicant: Steven A. Sundberg ,  J. Wallace Parce ,  Calvin Y. H. Chow

Inventor： Steven A. Sundberg ,  J. Wallace Parce ,  Calvin Y. H. Chow

IPC: G01N1/10 ,  B01L3/02

CPC classification number: G01N27/44791 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00826 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00853 ,  B01J2219/00891 ,  B01J2219/00909 ,  B01L3/0244 ,  B01L3/0262 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/502753 ,  B01L2200/027 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0487 ,  B01L2400/0688 ,  G01N27/44743 ,  G01N2035/00237 ,  G01N2035/1037 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/2575

Abstract: Fluid introduction is facilitated through the use of a port which extends entirely through a microfluidic substrate. Capillary forces can be used to retain the fluid within the port, and a series of samples or other fluids may be introduced through a single port by sequentially blowing the fluid out through the substrate and replacing the removed fluid with an alternate fluid, or by displacing the fluid in part with additional fluid. In another aspect, microfluidic substrates have channels which varying in cross-sectional dimension so that capillary action spreads a fluid only within a limited portion of the channel network. In yet another aspect, the introduction ports may include a multiplicity of very small channels leading from the port to a fluid channel, so as to filter out particles or other contaminants which might otherwise block the channel at the junction between the channel and the introduction port.

公开(公告)号：US06551836B1

公开(公告)日：2003-04-22

申请号：US09442073

申请日：1999-11-15

Applicant: Andrea W. Chow ,  Anne R. Kopf-Sill ,  J. Wallace Parce ,  Steven A. Sundberg

Inventor： Andrea W. Chow ,  Anne R. Kopf-Sill ,  J. Wallace Parce ,  Steven A. Sundberg

IPC: G01N27447

CPC classification number: B01L3/50273 ,  B01F13/0059 ,  B01L3/502753 ,  B01L3/502784 ,  B01L2200/0605 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0487 ,  G01N27/44743 ,  G01N27/44791 ,  G01N2001/4038 ,  Y10T436/117497 ,  Y10T436/25375 ,  Y10T436/2575

Abstract: Microfluidic devices for performing integrated reaction and separation operations. The devices have a planar substrate having a first surface with an integrated channel network disposed therein. The reaction region in the integrated microscale channel network has a mixture of at least first and second reactants located therein, wherein the mixture interacts to produce one or more products. The reaction region is configured to maintain contact between the first and second reactants contained within it. The device also includes a separation region in the integrated channel network, where the separation region is configured to separate the first reactant from the product, when the first reactant and product are flowing through the separation region.

Abstract translation: 用于进行集成反应和分离操作的微流体装置。 这些装置具有平面基板，其具有设置在其中的集成通道网络的第一表面。 集成微量通道网络中的反应区域具有位于其中的至少第一和第二反应物的混合物，其中混合物相互作用以产生一种或多种产物。 反应区域被配置为保持包含在其中的第一和第二反应物之间的接触。 该装置还包括在集成通道网络中的分离区域，其中当第一反应物和产物流过分离区域时，分离区域被配置为将第一反应物与产物分离。

公开(公告)号：US5252743A

公开(公告)日：1993-10-12

申请号：US612671

申请日：1990-11-13

Applicant: Ronald W. Barrett ,  Michael C. Pirrung ,  Lubert Stryer ,  Christopher P. Holmes ,  Steven A. Sundberg

Inventor： Ronald W. Barrett ,  Michael C. Pirrung ,  Lubert Stryer ,  Christopher P. Holmes ,  Steven A. Sundberg

IPC: C07D233/38 ,  C07D491/048 ,  C07D495/04 ,  C40B40/06 ,  G01N33/543 ,  G01N33/552 ,  C07D235/02

CPC classification number: C07D495/04 ,  C07D233/38 ,  G01N33/54306 ,  G01N33/54353 ,  G01N33/552 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00659 ,  B01J2219/00722 ,  C40B40/06 ,  Y02P20/55

Abstract: Methods and compositions are described for immobilizing anti-ligands, such as antibodies or antigens, hormones or hormone receptors, oligonucleotides, and polysaccharides on surfaces of solid substrates for various uses. The methods provide surfaces covered with caged binding members which comprise protecting groups capable of being removed upon application of a suitable energy source. Spatially addressed irradiation of predefined regions on the surface permits immobilization of anti-ligands at the activated regions on the surface. Cycles of irradiation on different regions of the surface and immobilization of different anti-ligands allows formation of an immobilized matrix of anti-ligands at defined sites on the surface. The immobilized matrix of anti-ligands permits simultaneous screenings of a liquid sample for ligands having high affinities for certain anti-ligands of the matrix. A preferred embodiment of the invention involves attaching photoactivatable biotin derivatives to a surface. Photolytic activation of the biotin derivatives forms biotin analogs having strong binding affinity for avidin. Biotinylated anti-ligands can be immobilized on activated regions of the surface previously treated with avidin.

Abstract translation: 描述了用于在固体底物的表面上固定抗配体如抗体或抗原，激素或激素受体，寡核苷酸和多糖的方法和组合物用于各种用途。 这些方法提供了覆盖有笼式结合构件的表面，其包括在施加合适的能量源时能够被去除的保护基团。 表面上预定区域的空间照射辐射允许在表面上的活化区域上固定抗配体。 表面不同区域的照射周期和不同抗配体的固定允许在表面上限定的位点形成固定的抗配体基团。 抗配体的固定化基质允许对基质的某些抗配体具有高亲和力的配体的液体样品的同时筛选。 本发明的优选实施方案涉及将可光活化的生物素衍生物连接到表面。 生物素衍生物的光解活化形成对抗生物素蛋白具有强结合亲和力的生物素类似物。 生物素化的抗配体可以固定在先前用抗生物素蛋白处理的表面的活化区上。

公开(公告)号：US08273242B2

公开(公告)日：2012-09-25

申请号：US13273934

申请日：2011-10-14

Applicant: Steven A. Sundberg ,  Xing Su ,  Grace Credo

Inventor： Steven A. Sundberg ,  Xing Su ,  Grace Credo

IPC: G01N27/327 ,  G01N27/26 ,  G01N27/447 ,  C12N11/02 ,  C12Q1/00

CPC classification number: C12N15/1006

Abstract: Embodiments of the invention provide devices and methods for extracting nucleic acid molecules from solution using electric fields. The structures and methods of embodiments of the invention are suited to incorporation into micro and nano fluidic devices, such as lab-on-a-chip devices and micro total analysis systems.

Abstract translation: 本发明的实施方案提供了使用电场从溶液中提取核酸分子的装置和方法。 本发明的实施例的结构和方法适合于并入微纳米流体装置，例如片上实验室装置和微总体分析系统。

公开(公告)号：US07364706B2

公开(公告)日：2008-04-29

申请号：US11617799

申请日：2006-12-29

Applicant: Steven A. Sundberg ,  J. Wallace Parce ,  Calvin Y. H. Chow

Inventor： Steven A. Sundberg ,  J. Wallace Parce ,  Calvin Y. H. Chow

IPC: G01N1/10 ,  B01L3/02

CPC classification number: G01N27/44791 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00826 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00853 ,  B01J2219/00891 ,  B01J2219/00909 ,  B01L3/0244 ,  B01L3/0262 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/502753 ,  B01L2200/027 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0487 ,  B01L2400/0688 ,  G01N27/44743 ,  G01N2035/00237 ,  G01N2035/1037 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/2575

Abstract: Fluid introduction is facilitated through the use of a port which extends entirely through a microfluidic substrate. Capillary forces can be used to retain the fluid within the port, and a series of samples or other fluids may be introduced through a single port by sequentially blowing the fluid out through the substrate and replacing the removed fluid with an alternate fluid, or by displacing the fluid in part with additional fluid. In another aspect, microfluidic substrates have channels which varying in cross-sectional dimension so that capillary action spreads a fluid only within a limited portion of the channel network. In yet another aspect, the introduction ports may include a multiplicity of very small channels leading from the port to a fluid channel, so as to filter out particles or other contaminants which might otherwise block the channel at the junction between the channel and the introduction port.