-
1.发明授权Methods for determination of single nucleic acid polymorphisms using bioelectronic microchip 有权使用生物电子微芯片测定单核酸多态性的方法公开(公告)号：US06468742B2
公开(公告)日：2002-10-22
申请号：US09291129
申请日：1999-04-12
申请人： Michael I. Nerenberg , David M. Canter , Ray R. Radtkey , Ling Wang , James P. O'connell , Ronald G. Sosnowski
发明人： Michael I. Nerenberg , David M. Canter , Ray R. Radtkey , Ling Wang , James P. O'connell , Ronald G. Sosnowski
IPC分类号： C12Q168
CPC分类号： H01L25/50 , B01J19/0046 , B01J19/0093 , B01J2219/00315 , B01J2219/00317 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00621 , B01J2219/00637 , B01J2219/00653 , B01J2219/00659 , B01J2219/00686 , B01J2219/00689 , B01J2219/00713 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B01L3/502761 , B01L3/5085 , B01L2300/0636 , B01L2300/0645 , B82Y5/00 , B82Y10/00 , C07B2200/11 , C07H21/00 , C07K1/04 , C07K1/045 , C07K1/047 , C12Q1/6844 , C12Q2600/156 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , G11C13/0014 , G11C13/0019 , G11C13/04 , G11C19/00 , H01L2924/0002 , C12Q2565/607 , C12Q2565/513 , H01L2924/00
摘要： Methods are provided for the analysis and determination of the nature of single nucleic acid polymorphisms (SNPs) in a genetic target. In one method of this invention, the nature of the SNPs in the genetic target is determined by the steps of providing a plurality of hybridization complexes arrayed on a plurality of test sites on an electronically bioactive microchip, where the hybridization complex includes at least a nucleic acid target containing a SNP, a stabilizer probe having a sequence complementary to the target sequence and/or reporter probe, and a reporter probe having a selected sequence complementary to either the stabilizer or the same target sequence strand wherein a selected sequence of the reporter includes either a wild type nucleotide or a nucleotide corresponding to the SNP of the target. In accordance with the invention, the stabilizer, reporter and target amplicons are hybridized using electronic assistance of the microchip system such that base-stacking energies are utilized in discerning among other identifying indicators, the presence of wild type or polymorphism sequence. Applications include disease diagnostics, such as for the identification of polymorphisms in structural genes, regulatory regions, antibiotic or chemotherapeutic resistance conferring regions, or for SNPs associated with speciation or used for determination of genetic linkage.
摘要翻译： 提供了用于分析和确定遗传靶标中单个核酸多态性（SNPs）的性质的方法。 在本发明的一种方法中，通过提供阵列在电子生物活性微芯片上的多个测试位点上的多个杂交复合物的步骤来确定遗传靶标中的SNP的性质，其中杂交复合物包括至少一个核酸 含有SNP的酸性靶，具有与靶序列互补的序列的稳定剂探针和/或报道探针，以及具有与稳定剂或相同靶序列链互补的选定序列的报告基因探针，其中所述报告基因的选定序列包括 野生型核苷酸或对应于靶标SNP的核苷酸。 根据本发明，使用微芯片系统的电子辅助将稳定剂，报告子和靶扩增子杂交，使得用于识别其他鉴定指示剂的碱基堆积能量，存在野生型或多态性序列。 应用包括疾病诊断，例如用于鉴定结构基因，调节区域，抗生素或化学抗性赋予区域中的多态性，或与形态相关或用于确定遗传连锁的SNP。
-
2.发明授权Methods for determination of single nucleic acid polymorphisms using a bioelectronic microchip 失效使用生物电子微芯片测定单核酸多态性的方法公开(公告)号：US07582421B2
公开(公告)日：2009-09-01
申请号：US10245206
申请日：2002-09-16
申请人： Ronald G. Sosnowski , Michael I. Nerenberg , David M. Canter , Ray R. Radtkey , Ling Wang , James P. O'Connell
发明人： Ronald G. Sosnowski , Michael I. Nerenberg , David M. Canter , Ray R. Radtkey , Ling Wang , James P. O'Connell
CPC分类号： H01L25/50 , B01J19/0046 , B01J19/0093 , B01J2219/00315 , B01J2219/00317 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/0063 , B01J2219/00653 , B01J2219/00659 , B01J2219/00686 , B01J2219/00689 , B01J2219/00713 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B01L3/5027 , B01L2300/0636 , B01L2300/0645 , B82Y5/00 , B82Y10/00 , C07B2200/11 , C07H21/00 , C07K1/04 , C07K1/045 , C07K1/047 , C12Q1/6813 , C12Q1/6816 , C12Q1/6825 , C12Q1/6827 , C12Q1/6832 , C12Q1/6837 , C12Q1/6876 , C12Q2600/156 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , G11C13/0014 , G11C13/0019 , G11C13/04 , G11C19/00 , H01L2924/0002 , Y02A90/26 , C12Q2565/501 , C12Q2565/607 , C12Q2537/162 , C12Q2565/515 , H01L2924/00
摘要： This application includes methods for detecting single nucleotide polymorphisms (SNPs) in a sample using an electronically addressable microchip having a plurality of test sites. A sample nucleic acid is electronically biased, concentrated at, and immobilized to a test site on the microchip. A mixture comprising a first labeled probe and a second labeled probe is electronically hybridized to the sample nucleic acid to form first or second hybridized complexes. The first labeled probe is perfectly complementary to the first sample nucleic acid and the second labeled probe is complementary to the sample nucleic acid and contains a nucleotide that forms a mismatch with the nucleotide at the site of the polymorphism. The first or second hybridized complexes are detected by determining a signal intensity of the label of the first or second probe.
摘要翻译： 该应用包括使用具有多个测试位点的电子可寻址微芯片检测样品中的单核苷酸多态性（SNP）的方法。 将样品核酸电子偏置，浓缩并固定在微芯片上的测试位点。 将包含第一标记探针和第二标记探针的混合物与样品核酸电子杂交以形成第一或第二杂交复合物。 第一标记探针与第一样品核酸完全互补，第二标记探针与样品核酸互补，并含有与多态性位点处的核苷酸形成错配的核苷酸。 通过确定第一或第二探针的标记的信号强度来检测第一或第二杂交复合物。
-
3.发明授权
公开(公告)号：US6051380A
公开(公告)日：2000-04-18
申请号：US986065
申请日：1997-12-05
申请人： Ronald G. Sosnowski , William F. Butler , Eugene Tu , Michael I. Nerenberg , Michael J. Heller , Carl F. Edman
发明人： Ronald G. Sosnowski , William F. Butler , Eugene Tu , Michael I. Nerenberg , Michael J. Heller , Carl F. Edman
IPC分类号： C12N15/09 , B01J19/00 , B01L3/00 , C07B61/00 , C07H21/00 , C07K1/04 , C12Q1/68 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , G11C13/02 , G11C19/00 , H01L21/336 , H01L21/98 , H01L29/78
CPC分类号： H01L29/7834 , B01J19/0046 , B01J19/0093 , B01L3/502707 , B01L3/50273 , B01L3/502761 , B01L3/5085 , B82Y10/00 , B82Y5/00 , C07H21/00 , C07K1/04 , C07K1/045 , C07K1/047 , C12Q1/6825 , C12Q1/6832 , C12Q1/6837 , G11C13/0014 , G11C13/0019 , G11C19/00 , H01L25/50 , H01L29/6656 , H01L29/6659 , H01L29/66628 , B01J2219/00315 , B01J2219/00317 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00635 , B01J2219/00637 , B01J2219/00644 , B01J2219/00653 , B01J2219/00659 , B01J2219/00686 , B01J2219/00689 , B01J2219/00713 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B01L2200/0647 , B01L2200/10 , B01L2300/0636 , B01L2300/0645 , B01L2400/0415 , B01L2400/0421 , C07B2200/11 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , G11C13/04 , H01L2224/95145 , H01L2224/95147 , H01L2924/01019 , H01L2924/01025 , H01L2924/01046 , H01L2924/01078 , H01L2924/01079 , H01L2924/10253
摘要： A self-addressable, self-assembling microelectronic device is designed and fabricated to actively carry out and control multi-step and multiplex molecular biological reactions in microscopic formats. These reactions include nucleic acid hybridizations, antibody/antigen reactions, diagnostics, and biopolymer synthesis. The device can be fabricated using both microlithographic and micro-machining techniques. The device can electronically control the transport and attachment of specific binding entities to specific microlocations. The specific binding entities include molecular biological molecules such as nucleic acids and polypeptides. The device can subsequently control the transport and reaction of analytes or reactants at the addressed specific microlocations. The device is able to concentrate analytes and reactants, remove non-specifically bound molecules, provide stringency control for DNA hybridization reactions, and improve the detection of analytes. The device can be electronically replicated.
摘要翻译： 设计制造了一种自寻址的自组装微电子器件，主动执行和控制微步形式的多步分子生物反应。 这些反应包括核酸杂交，抗体/抗原反应，诊断和生物聚合物合成。 该器件可以使用微光刻和微加工技术制造。 该装置可以电子控制特定结合实体到特定微位置的转运和附着。 特异性结合实体包括分子生物学分子，例如核酸和多肽。 该装置随后可以控制分析物或反应物在所寻址的特定微位置的转运和反应。 该装置能够浓缩分析物和反应物，去除非特异性结合的分子，为DNA杂交反应提供严格控制，并改善分析物的检测。 该设备可以电子复制。
-
4.发明授权公开(公告)号：US06531302B1
公开(公告)日：2003-03-11
申请号：US09290000
申请日：1999-04-12
申请人： Michael I. Nerenberg , Carl F. Edman , Lorelei P. Westin , Lana L. Feng , Geoffrey C. Landis , Ronald G. Sosnowski
发明人： Michael I. Nerenberg , Carl F. Edman , Lorelei P. Westin , Lana L. Feng , Geoffrey C. Landis , Ronald G. Sosnowski
IPC分类号： C12Q168
CPC分类号： C12Q1/6844 , C12Q1/6837 , C12Q2531/119 , C12Q2565/607 , C12Q2525/313
摘要： Methods and compositions of matter are provided for the strand displacement amplification of target nucleic acids of interest using primer pair sets that are anchored to electronically addressable capture sites on a microarray. The primer pair sets may be individually bound to the capture sites or may comprise a unique branched primer pair moiety. The anchored primers allow for the simultaneous multiplex capture, amplification and detection of a target nucleic acid derived from any sample source.
摘要翻译： 提供了物质的方法和组成，用于使用锚定在微阵列上的电子可寻址捕获位点的引物对组进行目标核酸的链置换扩增。 引物对集合可以单独地结合到捕获位点或可以包含独特的支链引物对部分。 锚定的引物允许同时多重捕获，扩增和检测衍生自任何样品来源的靶核酸。
-
5.发明授权公开(公告)号：US06518022B1
公开(公告)日：2003-02-11
申请号：US09444539
申请日：1999-11-22
申请人： Ronald G. Sosnowski , William F. Butler , Eugene Tu , Michael I. Nerenberg , Michael J. Heller , Carl F. Edman
发明人： Ronald G. Sosnowski , William F. Butler , Eugene Tu , Michael I. Nerenberg , Michael J. Heller , Carl F. Edman
IPC分类号： C12Q168
CPC分类号： H01L29/7834 , B01J19/0046 , B01J19/0093 , B01J2219/00315 , B01J2219/00317 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00635 , B01J2219/00637 , B01J2219/00644 , B01J2219/00653 , B01J2219/00659 , B01J2219/00686 , B01J2219/00689 , B01J2219/00713 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B01L3/502707 , B01L3/50273 , B01L3/502761 , B01L3/5085 , B01L2200/0647 , B01L2200/10 , B01L2300/0636 , B01L2300/0645 , B01L2400/0415 , B01L2400/0421 , B82Y5/00 , B82Y10/00 , C07B2200/11 , C07H21/00 , C07K1/04 , C07K1/045 , C07K1/047 , C12Q1/6825 , C12Q1/6832 , C12Q1/6837 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , G11C13/0014 , G11C13/0019 , G11C13/04 , G11C19/00 , H01L25/50 , H01L29/6656 , H01L29/6659 , H01L29/66628 , H01L2224/95145 , H01L2224/95147 , H01L2924/01019 , H01L2924/01025 , H01L2924/01046 , H01L2924/01078 , H01L2924/01079 , H01L2924/10253 , C12Q2565/515 , C12Q2565/607 , H01L2924/00
摘要： A self-addressable, self-assembling microelectronic device is designed and fabricated to actively carry out and control multi-step and multiplex molecular biological reactions in microscopic formats. These reactions include nucleic acid hybridizations, antibody/antigen reactions, diagnostics, and biopolymer synthesis. The device can be fabricated using both microlithographic and micro-machining techniques. The device can electronically control the transport and attachment of specific binding entities to specific microlocations. The specific binding entities include molecular biological molecules such as nucleic acids and polypeptides. The device can subsequently control the transport and reaction of analytes or reactants at the addressed specific microlocations. The device is able to concentrate analytes and reactants, remove non-specifically bound molecules, provide stringency control for DNA hybridization reactions, and improve the detection of analytes. The device can be electronically replicated.
摘要翻译： 设计制造了一种自寻址的自组装微电子器件，主动执行和控制微步形式的多步分子生物反应。 这些反应包括核酸杂交，抗体/抗原反应，诊断和生物聚合物合成。 该器件可以使用微光刻和微加工技术制造。 该装置可以电子控制特定结合实体到特定微位置的转运和附着。 特异性结合实体包括分子生物学分子，例如核酸和多肽。 该装置随后可以控制分析物或反应物在所寻址的特定微位置的转运和反应。 该装置能够浓缩分析物和反应物，去除非特异性结合的分子，为DNA杂交反应提供严格控制，并改善分析物的检测。 该设备可以电子复制。
-
6.发明授权公开(公告)号：US08114589B2
公开(公告)日：2012-02-14
申请号：US11726520
申请日：2007-03-22
申请人： Ronald G. Sosnowski , William F. Butler , Eugene Tu , Michael I. Nerenberg , Michael J. Heller , Carl F. Edman
发明人： Ronald G. Sosnowski , William F. Butler , Eugene Tu , Michael I. Nerenberg , Michael J. Heller , Carl F. Edman
CPC分类号： H01L29/7834 , B01J19/0046 , B01J19/0093 , B01J2219/00315 , B01J2219/00317 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00635 , B01J2219/00637 , B01J2219/00644 , B01J2219/00653 , B01J2219/00659 , B01J2219/00686 , B01J2219/00689 , B01J2219/00713 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B01L3/502707 , B01L3/50273 , B01L3/502761 , B01L3/5085 , B01L2200/0647 , B01L2200/10 , B01L2300/0636 , B01L2300/0645 , B01L2400/0415 , B01L2400/0421 , B82Y5/00 , B82Y10/00 , C07B2200/11 , C07H21/00 , C07K1/04 , C07K1/045 , C07K1/047 , C12Q1/6825 , C12Q1/6832 , C12Q1/6837 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , G11C13/0014 , G11C13/0019 , G11C13/04 , G11C19/00 , H01L25/50 , H01L29/6656 , H01L29/6659 , H01L29/66628 , H01L2224/95145 , H01L2224/95147 , H01L2924/01019 , H01L2924/01025 , H01L2924/01046 , H01L2924/01078 , H01L2924/01079 , H01L2924/10253 , C12Q2565/515 , C12Q2565/607 , H01L2924/00
摘要： A method for electronically stabilizing hybridization of nucleic acids bound at a test site of a microelectronic device is described. First and second negatively charged nucleic acids are provided, the second nucleic acid being bound to the test site. A zwitterionic buffer having a conductance of less than 100 mS/cm is applied to the microelectronic device. A current is applied to the test site to positively bias the test site, such that the first negatively charged nucleic acid is transported to the positively biased test site having the bound the second negatively charged nucleic acid. At the test site, the first and second negatively charged nucleic acids hybridize. The zwitterionic buffer acquires a net positive charge under influence of the current, such that the positively charged zwitterionic buffer stabilizes the hybridization by reducing the repulsion between the first and second negatively charged nucleic acids.
摘要翻译： 描述了用于电子稳定在微电子器件的测试位点处结合的核酸杂交的方法。 提供第一和第二带负电荷的核酸，第二核酸与测试部位结合。 将具有小于100mS / cm 2的电导的两性离子缓冲液施加到微电子器件。 将电流施加到测试部位以使测试部位正偏置，使得将第一带负电荷的核酸转运到具有结合第二带负电荷核酸的正偏压测试位点。 在测试位点，第一和第二带负电荷的核酸杂交。 两性离子缓冲液在电流影响下获得净正电荷，使得带正电的两性离子缓冲液通过降低第一和第二带负电的核酸之间的排斥来稳定杂交。
-
7.发明授权Multiplex amplification and separation of nucleic acid sequences on a bioelectronic microchip using asymmetric structures 有权使用不对称结构在生物电子微芯片上多重扩增和分离核酸序列公开(公告)号：US06309833B1
公开(公告)日：2001-10-30
申请号：US09290452
申请日：1999-04-12
IPC分类号： C12Q168
CPC分类号： C12Q1/6846 , C12Q1/6825 , C12Q1/6837 , C12Q1/6853 , C12Q2521/301 , C12Q2537/101 , C12Q2565/607 , C12Q2565/501 , C12Q2531/107 , C12Q2531/119 , C12Q2565/543
摘要： A method for amplifying nucleic acids is provided wherein detection of amplified species is enhanced by the use of asymmetric amplification. Such amplification is made asymmetric by using divergent ratios of amplification primers or by using non-extending and/or non-cleavable amplification primers. Detection of the amplicons is improved because maintenance of single stranded species of amplicons during amplification facilitates their direct capture by immobilized probes without having to include denaturing steps.
摘要翻译： 提供了用于扩增核酸的方法，其中通过使用不对称扩增增强了扩增物种的检测。 通过使用扩增引物的扩散比例或通过使用不扩增和/或不可切割的扩增引物使这种扩增变得不对称。 扩增子的检测得到改善，因为在扩增过程中单链扩增子的维持促进了其通过固定化探针的直接捕获，而不必包括变性步骤。
-
8.发明授权Suppression of nuclear factor-κB dependent processes using oligonucleotides 失效使用寡核苷酸抑制核因子 - kgr B依赖过程公开(公告)号：US07704968B2
公开(公告)日：2010-04-27
申请号：US11888342
申请日：2007-07-30
CPC分类号： C12N15/113 , A01K2217/05 , A61K31/7125 , A61K38/00 , A61K48/00 , C12N15/1132 , C12N2310/111 , C12N2310/315 , C12N2310/341
摘要： Antisense oligonucleotides which hybridize with nuclear factor-κB (NF-κB) mRNA and methods of using these oligonucleotides.
摘要翻译： 与核因子-κB（NF-κB）mRNA杂交的反义寡核苷酸和使用这些寡核苷酸的方法。
-
9.发明申请Suppression of Nuclear Factor-KappaB Dependent Processes Using Oligonucleotides 失效使用寡核苷酸抑制核因子-κB依赖性过程公开(公告)号：US20080274995A1
公开(公告)日：2008-11-06
申请号：US12115373
申请日：2008-05-05
IPC分类号： A61K31/711 , A61K31/7105 , A61P35/00
CPC分类号： C12N15/113 , A01K2217/05 , A61K31/7125 , A61K38/00 , A61K48/00 , C12N15/1132 , C12N2310/111 , C12N2310/315 , C12N2310/341
摘要： Antisense oligonucleotides which hybridize with nuclear factor-κB (NF-κB) mRNA and methods of using these oligonucleotides.
摘要翻译： 与核因子κB（NF-kappaB）mRNA杂交的反义寡核苷酸和使用这些寡核苷酸的方法。
-
公开(公告)号：US06589742B2
公开(公告)日：2003-07-08
申请号：US09954594
申请日：2001-09-17
IPC分类号： C12Q168
CPC分类号： C12Q1/6846 , C12Q1/6825 , C12Q1/6837 , C12Q1/6853 , C12Q2521/301 , C12Q2537/101 , C12Q2565/607 , C12Q2565/501 , C12Q2531/107 , C12Q2531/119 , C12Q2565/543
摘要： A method for amplifying nucleic acids is provided wherein detection of amplified species is enhanced by the use of asymmetric amplification. Such amplification is made asymmetric by using divergent ratios of amplification primers or by using non-extending and/or non-cleavable amplification primers. Detection of the amplicons is improved because maintenance of single stranded species of amplicons during amplification facilitates their direct capture by immobilized probes without having to include denaturing steps.
-
-
-
-
-
-
-
-
-
搜索结果
15 条记录 (耗时 0.036 秒)
