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    CELL COLLECTION SYSTEM
    3.
    发明申请
    CELL COLLECTION SYSTEM 审中-公开
    细胞收集系统

    公开(公告)号：US20140065704A1

    公开(公告)日：2014-03-06

    申请号：US14111036

    申请日：2012-04-10

    申请人： Masataka Shirai ,  Hiroyuki Tsunoda ,  Hiroko Matsunaga ,  Kenko Uchida

    发明人： Masataka Shirai ,  Hiroyuki Tsunoda ,  Hiroko Matsunaga ,  Kenko Uchida

    IPC分类号： C12M1/26

    CPC分类号： C12M33/04 ,  C12M47/04

    摘要： The present invention provides a simple and highly reliable cell collection system with high throughput and improved in cell collection efficiency. In the present invention, one or more pores are formed in a cell collection plate. One surface of the plate can be directly introduced in e.g., a petri dish, so as to be in contact with a solution containing cells. In this case, means for obtaining an optical image of collected cells from its rear surface is provided to improve reliability and convenience during a cell collection process. Alternatively, the vicinities of the pores in the cell collection plate are only hydrophilized and the other region is made water repellent to improve the efficiency of cell collection.

    摘要翻译： 本发明提供了一种具有高通量并提高了细胞收集效率的简单且高度可靠的细胞收集系统。 在本发明中，在细胞收集板中形成一个以上的孔。 板的一个表面可以直接引入例如陪替氏培养皿中，以便与含有细胞的溶液接触。 在这种情况下，提供用于从其后表面获得收集的细胞的光学图像的装置，以提高细胞收集过程中的可靠性和便利性。 或者，细胞收集板中的孔附近仅被亲水化，另一区域被制成防水剂，以提高细胞收集的效率。

    Probe synthesis method for nucleic acid detection
    6.
    发明申请
    Probe synthesis method for nucleic acid detection 审中-公开
    探针合成方法用于核酸检测

    公开(公告)号：US20070238125A1

    公开(公告)日：2007-10-11

    申请号：US11783124

    申请日：2007-04-06

    申请人： Chihiro Uematsu ,  Chifumi Gouda ,  Masataka Shirai ,  Takashi Anazawa

    发明人： Chihiro Uematsu ,  Chifumi Gouda ,  Masataka Shirai ,  Takashi Anazawa

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C12M3/00

    CPC分类号： C12P19/34

    摘要： The present invention relates to a probe synthesis method and a probe synthesis kit for nucleic acid detection, which are intended for the fluorophore labeling and detection of a target gene. In this method, a probe unit for analyte nucleic acid recognition having a base sequence specific to an analyte nucleic acid and plural labeled probe units each having a fluorophore labeled internal base are hybridized to oligonucleotides complementary thereto, and the adjacent probe units are bonded by ligation. As a result, an analytical probe with increased fluorescence intensity is synthesized easily and inexpensively, while the number of fluorophore labels is controlled.

    摘要翻译： 本发明涉及用于靶基因的荧光标记和检测的用于核酸检测的探针合成方法和探针合成试剂盒。 在该方法中，具有分析物核酸特异性的碱基序列的分析物核酸识别用探针单元和各自具有荧光团标记的内部碱基的多个标记的探针单元与互补的寡核苷酸杂交，并且通过连接键合相邻的探针单元 。 结果，容易且廉价地合成了增加荧光强度的分析探针，同时控制了荧光标记的数量。

    Tag-Sequence-Attached Two-Dimensional CDNA Library Device, and Gene Expression Analysis Method and Gene Expression Analysis Apparatus Each Utilizing Same
    8.
    发明申请
    Tag-Sequence-Attached Two-Dimensional CDNA Library Device, and Gene Expression Analysis Method and Gene Expression Analysis Apparatus Each Utilizing Same 审中-公开
    标签序列连接的二维CDNA文库装置及基因表达分析方法及基因表达分析装置

    公开(公告)号：US20150167063A1

    公开(公告)日：2015-06-18

    申请号：US14418135

    申请日：2012-07-30

    申请人： Masataka Shirai ,  Hideki Kambara ,  Kiyomi Taniguchi ,  Maiko Tanabe

    发明人： Masataka Shirai ,  Hideki Kambara ,  Kiyomi Taniguchi ,  Maiko Tanabe

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6837 ,  C12N15/1096 ,  C12Q2525/155 ,  C12Q2565/537 ,  C12Q2565/601

    摘要： The present invention relates to a method, a device, and an apparatus for analyzing the expression of a gene in single cells. Specifically, the present invention relates to: a device for gene expression analysis, characterized by including a support, in which a nucleic acid probe having a test nucleic acid capture sequence and a known sequence, and further containing a cell recognition tag sequence which differs depending on the difference in position on the surface of the support or in the vicinity of the surface thereof, and a common primer sequence having a known sequence is two-dimensionally distributed and immobilized on the surface of the support or in the vicinity of the surface thereof; and a method and an apparatus using the device for gene expression analysis.

    摘要翻译： 本发明涉及用于分析单细胞中基因表达的方法，装置和装置。 具体地说，本发明涉及：用于基因表达分析的装置，其特征在于，包括载体，其中具有测试核酸捕获序列和已知序列的核酸探针，并且还包含不同的细胞识别标签序列 关于载体表面或其表面附近的位置差异，具有已知序列的常见引物序列被二维分布和固定在载体的表面上或其表面附近 ; 以及使用该基因表达分析装置的方法和装置。

    METHOD FOR TRANSFERRING DROPLET
    9.
    发明申请
    METHOD FOR TRANSFERRING DROPLET 有权
    用于转移滴液的方法

    公开(公告)号：US20070207064A1

    公开(公告)日：2007-09-06

    申请号：US11675749

    申请日：2007-02-16

    申请人： Yoshinobu Kohara ,  Masataka Shirai ,  Hideyuki Noda ,  Tomoyuki Sakai ,  Kenko Uchida

    发明人： Yoshinobu Kohara ,  Masataka Shirai ,  Hideyuki Noda ,  Tomoyuki Sakai ,  Kenko Uchida

    IPC分类号： B01L3/02

    CPC分类号： B01L3/0244 ,  B01F7/00258 ,  B01F11/0071 ,  B01F11/0258 ,  B01F13/0071 ,  B01L3/0251 ,  B01L3/0255 ,  B01L3/50 ,  B01L2300/0845 ,  B01L2300/089 ,  B01L2300/12 ,  G01N21/03 ,  G01N21/64 ,  G01N2021/0346 ,  G01N2021/035 ,  G01N2035/1037 ,  G01N2035/1046

    摘要： The invention provides a method in which an annular or spiral droplet holder formed of wire is used to hold a droplet in a state of being hung therefrom or being contained therein. A means for moving the droplet holder is added to the droplet holder to enable droplet transfer. To merge two droplets, they are brought into contact. To drip the droplet held by a droplet holder formed of wire, the droplet holder is deformed using an external force. A light path which passes through a droplet is set to enable optical measurement. The present invention enables inexpensive, simple droplet transfer. An inexpensive, simple configuration for handling droplets in the fields of chemical analysis, biochemical analysis, and automatic blood analysis can be realized according to the present invention.

    摘要翻译： 本发明提供一种方法，其中使用由线形成的环形或螺旋液滴保持器将其悬挂在其中或被容纳在其中的状态。 用于移动液滴保持器的装置被添加到液滴保持器以使液滴转移。 为了合并两个液滴，它们被接触。 为了滴落由线形成的液滴保持器保持的液滴，液滴保持器使用外力而变形。 设置通过液滴的光路以进行光学测量。 本发明可实现廉价的简单的液滴转印。 根据本发明，可以实现用于处理化学分析，生化分析和自动血液分析领域中的液滴的廉价，简单的构造。

    Optical semiconductor device
    10.
    发明授权
    Optical semiconductor device 有权
    光半导体器件

    公开(公告)号：US07223993B2

    公开(公告)日：2007-05-29

    申请号：US11210760

    申请日：2005-08-25

    申请人： Kouji Nakahara ,  Makoto Kudo ,  Shigehisa Tanaka ,  Masataka Shirai

    发明人： Kouji Nakahara ,  Makoto Kudo ,  Shigehisa Tanaka ,  Masataka Shirai

    IPC分类号： H01L29/06

    CPC分类号： H01S5/34313 ,  B82Y20/00 ,  G02F1/01708 ,  G02F2001/01766 ,  H01S5/0265 ,  H01S5/0287 ,  H01S5/12 ,  H01S5/1221 ,  H01S5/227 ,  H01S5/305 ,  H01S5/3054 ,  H01S5/3406 ,  H01S5/34306 ,  H01S5/34366 ,  Y10S438/962

    摘要： In the semiconductor laser or electro-absorption optical modulator that includes strained quantum well layers as active layers, making laser characteristics or modulator characteristics adequate has seen the respective limits since band structures, especially, ΔEc and ΔEv, have been unable to be adjusted independently.This invention is constructed by stacking an n-type InGaAlAs-GRIN-SCH layer 3, an MQW layer 4, a p-type InGaAlAs-GRIN-SCH layer 5, a p-type InAlAs electron-stopping layer 6, and others, in that order, on an n-type InP wafer 1; wherein the MQW layer 4 includes InGaAlAs-strained quantum well layers and InGaAlAsSb-formed barrier layers each having strain of an opposite sign to the strain applied to the quantum well layers.

    摘要翻译： 在包括应变量子阱层作为有源层的半导体激光器或电吸收光学调制器中，由于带结构，特别是DeltaEc和DeltaEv已经不能独立地调整，所以使激光特性或调制器特性足够的已经看到相应的限制。 本发明通过将n型InGaAlAs-GRIN-SCH层3，MQW层4，p型InGaAlAs-GRIN-SCH层5，p型InAlAs电子停止层6等层叠而构成 在n型InP晶片1上的顺序; 其中MQW层4包括InGaAlAs应变的量子阱层和InGaAlAsSb形成的阻挡层，每个具有与施加到量子阱层的应变相反的符号的应变。