Method and apparatus for separating particles
    1.
    发明授权
    Method and apparatus for separating particles 失效
    分离颗粒的方法和装置

    公开(公告)号:US06224732B1

    公开(公告)日:2001-05-01

    申请号:US08734971

    申请日:1996-11-19

    IPC分类号: C25B900

    摘要: A dispersing medium containing plural types of particles is let to flow in a flow path formed in a flow cell. The flow cell is irradiated with interfering light to form interference fringes of a pattern of stripes. In another arrangement, light is two-dimensionally scanned at high speed so as to cross a flow in the flow path. The moving particles receive a braking force by the light gradient force whenever they pass through each irradiation light stripe. In that case, greater braking forces act on particles having larger sizes (or larger refractive indices) than on particles having smaller sizes (or smaller refractive indices). Accordingly, the particles receiving smaller braking forces pass through the irradiated position more rapidly, so that particles can be separated to flow in the order from the particles receiving smaller braking forces to the particles receiving greater braking forces.

    摘要翻译: 使含有多种粒子的分散介质在形成于流通池中的流路中流动。 用干涉光照射流动池,以形成条纹图案的干涉条纹。 在另一种布置中,光以高速二维地扫描,以便穿过流动路径中的流动。 每当它们通过每个照射光条纹时,移动的颗粒通过光梯度力接收制动力。 在这种情况下,与具有较小尺寸(或较小折射率)的颗粒相比,较大的制动力作用于具有较大尺寸(或更大折射率)的颗粒。 因此,接收较小制动力的颗粒更快地穿过照射位置,使得颗粒可以分离成从接收较小制动力的颗粒到接收更大制动力的颗粒的顺序流动。

    Method for specimen measurement
    10.
    发明授权
    Method for specimen measurement 失效
    样本测量方法

    公开(公告)号:US5601983A

    公开(公告)日:1997-02-11

    申请号:US395631

    申请日:1995-02-28

    IPC分类号: G01N33/543 G01N33/58 C12Q1/68

    摘要: In a method for measuring a specified component in a specimen by reacting the specimen with a first reagent formed by binding a substance active to the specified component, with carrier particles and a second reagent formed by labelling a substance active to the specified component with a first label, and measuring the substances in the complexes obtained in the reaction, there is disclosed a method featured by labelling the carrier particles with a second label different from the first label, and detecting the second label and then the first label utilizing the detection of the second label as a trigger.This method enables a highly precise measurement without the influence of noise components in the detection of specified trace components in the specimen, utilizing an antigen-antibody reaction or a nucleic acid hybridization.

    摘要翻译: 在通过使样本与通过结合对特定成分有活性的物质结合而形成的第一试剂与载体颗粒和通过将具有活性的物质标记在特定成分上而形成的第二试剂的方法中, 标记和测量在反应中获得的络合物中的物质,公开了一种特征在于用不同于第一标记的第二标记标记载体颗粒的方法,并且利用第二标记的检测来检测第二标记 第二个标签作为触发器。 该方法利用抗原抗体反应或核酸杂交,能够在不受噪声成分影响的情况下进行高精度测定。