公开(公告)号：US5370842A

公开(公告)日：1994-12-06

申请号：US979811

申请日：1992-11-20

申请人： Takeshi Miyazaki ,  Matsuomi Nishimura ,  Takayuki Yagi ,  Kazumi Tanaka ,  Toshikazu Ohnishi ,  Masanori Sakuranaga ,  Yoshito Yoneyama ,  Hidehito Takayama ,  Kazuo Isaka

发明人： Takeshi Miyazaki ,  Matsuomi Nishimura ,  Takayuki Yagi ,  Kazumi Tanaka ,  Toshikazu Ohnishi ,  Masanori Sakuranaga ,  Yoshito Yoneyama ,  Hidehito Takayama ,  Kazuo Isaka

IPC分类号： B01L3/00 ,  B01L3/02 ,  G01N15/14 ,  G01N21/03 ,  G01N33/543 ,  G01N35/00 ,  G01N35/08 ,  G01N35/10 ,  G01N21/00 ,  G01N21/85 ,  B01L21/85

CPC分类号： G01N15/1459 ,  B01L3/0268 ,  B01L3/502715 ,  G01N15/1484 ,  G01N21/0303 ,  G01N33/54366 ,  G01N35/08 ,  B01L2200/027 ,  B01L2200/0636 ,  B01L2300/0654 ,  B01L2300/0825 ,  B01L2400/0442 ,  B01L2400/0466 ,  G01N2035/00237 ,  G01N2035/1039 ,  G01N2035/1041

摘要： An injection port for injecting a sample, an accumulation portion for accumulating the injected sample having a reagent carrier enclosed, a flow passage portion, having a light receiving element at a measuring position located halfway thereof, for passing a reaction fluid having reacted with the reagent in the accumulation portion therethrough, a micro-pump having a feed action of the sample fluid within the flow passage portion and which is a heat generating element provided near a nozzle downstream of the measuring position of the flow passage portion are formed integrally and intensively as a cartridge by a producing method including a semiconductor fabrication process.

摘要翻译： 用于注射样品的注入口，用于积聚注入了试剂载体的注射样品的积聚部分，在位于其中途的测量位置处具有光接收元件的流路部分，用于使与反应物反应的反应流体 在其中的积聚部分中，具有在流动通道部分内具有样品流体的进给动作并且设置在流路部分的测量位置下游的喷嘴附近的发热元件的微型泵一体地集中地形成为 一种通过包括半导体制造工艺的制造方法的盒。

公开(公告)号：US5599502A

公开(公告)日：1997-02-04

申请号：US280132

申请日：1994-07-25

申请人： Takeshi Miyazaki ,  Matsuomi Nishimura ,  Kazuo Isaka ,  Kazumi Tanaka ,  Toshikazu Ohnishi ,  Yoshito Yoneyama ,  Hidehito Takayama

发明人： Takeshi Miyazaki ,  Matsuomi Nishimura ,  Kazuo Isaka ,  Kazumi Tanaka ,  Toshikazu Ohnishi ,  Yoshito Yoneyama ,  Hidehito Takayama

IPC分类号： F04F99/00 ,  B01L3/00 ,  B41J2/005 ,  F04B19/00 ,  F04B19/24 ,  G01N15/14 ,  G01N37/00 ,  G01N27/00 ,  G01N21/00 ,  G01N21/85

CPC分类号： B41J2/005 ,  B01L3/502715 ,  B01L3/50273 ,  F04B19/006 ,  F04B19/24 ,  B01L2300/0645 ,  B01L2300/0825 ,  B01L2300/0887 ,  B01L2300/1816 ,  B01L2300/1827 ,  B01L2300/1833 ,  B01L2300/1861 ,  B01L2400/0466

摘要： A minute flow path is filled with liquid so that the liquid may be supplied from an accumulating portion. Energy is imparted to the liquid exposed outwardly of an opening in the flow path by a heat generating element or by energy application to thereby heat and gasify the liquid. Thereupon, the liquid is supplied by an amount corresponding to the gasified liquid by capillary phenomenon through the flow path, and gasification is continuously effected, whereby a flow free of pulsating flow can be formed in the flow path.

摘要翻译： 一个微小的流路充满液体，从而可以从一个积聚部分提供液体。 通过发热元件或通过能量施加向流动路径中的开口向外暴露的液体赋予能量，从而加热和气化液体。 因此，通过流路通过毛细管现象向气化液体供给液体，连续进行气化，能够在流路中形成不产生脉动流的流动。

公开(公告)号：US5495105A

公开(公告)日：1996-02-27

申请号：US375253

申请日：1995-01-19

申请人： Matsuomi Nishimura ,  Kazuo Isaka ,  Tadashi Okamoto ,  Kazumi Tanaka ,  Toshikazu Onishi ,  Takeshi Miyazaki ,  Hidehito Takayama

发明人： Matsuomi Nishimura ,  Kazuo Isaka ,  Tadashi Okamoto ,  Kazumi Tanaka ,  Toshikazu Onishi ,  Takeshi Miyazaki ,  Hidehito Takayama

IPC分类号： G01N15/14 ,  G01N30/00 ,  G01N30/02 ,  G21K1/00 ,  H05H3/04 ,  G01B13/00 ,  G01N11/00

CPC分类号： H05H3/04 ,  G01N15/14 ,  G01N30/00 ,  G01N2015/149 ,  G01N30/02

摘要： A flow of liquid containing floating fine particles is formed in a flow path, thereby causing successive movement of the particles. A light beam having intensity distribution from a laser is focused on the liquid flow, whereby the particle is optically trapped at the irradiating position, thus being stopped against the liquid flow or being slowed by a braking force. This phenomenon is utilized in controlling the spacing of the particles in the flow or in separating the particles.

摘要翻译： 在流动路径中形成含有浮动细颗粒的液体流，从而引起颗粒的连续移动。 具有来自激光器的强度分布的光束被聚焦在液体流动上，由此将颗粒光学地捕获在照射位置，从而被阻止液体流动或被制动力减慢。 这种现象用于控制颗粒在流动中或分离颗粒的间距。

公开(公告)号：US5601983A

公开(公告)日：1997-02-11

申请号：US395631

申请日：1995-02-28

申请人： Hidehito Takayama ,  Matsuomi Nishimura ,  Kazumi Tanaka ,  Toshikazu Ohnishi ,  Takeshi Miyazaki

发明人： Hidehito Takayama ,  Matsuomi Nishimura ,  Kazumi Tanaka ,  Toshikazu Ohnishi ,  Takeshi Miyazaki

IPC分类号： G01N33/543 ,  G01N33/58 ,  C12Q1/68

CPC分类号： G01N33/582 ,  G01N33/585 ,  Y10S435/961 ,  Y10S435/968 ,  Y10S436/80 ,  Y10S436/804 ,  Y10S436/805 ,  Y10S436/823

摘要： In a method for measuring a specified component in a specimen by reacting the specimen with a first reagent formed by binding a substance active to the specified component, with carrier particles and a second reagent formed by labelling a substance active to the specified component with a first label, and measuring the substances in the complexes obtained in the reaction, there is disclosed a method featured by labelling the carrier particles with a second label different from the first label, and detecting the second label and then the first label utilizing the detection of the second label as a trigger.This method enables a highly precise measurement without the influence of noise components in the detection of specified trace components in the specimen, utilizing an antigen-antibody reaction or a nucleic acid hybridization.

摘要翻译： 在通过使样本与通过结合对特定成分有活性的物质结合而形成的第一试剂与载体颗粒和通过将具有活性的物质标记在特定成分上而形成的第二试剂的方法中， 标记和测量在反应中获得的络合物中的物质，公开了一种特征在于用不同于第一标记的第二标记标记载体颗粒的方法，并且利用第二标记的检测来检测第二标记 第二个标签作为触发器。 该方法利用抗原抗体反应或核酸杂交，能够在不受噪声成分影响的情况下进行高精度测定。

公开(公告)号：US5427959A

公开(公告)日：1995-06-27

申请号：US769366

申请日：1991-10-01

申请人： Matsuomi Nishimura ,  Kazumi Tanaka ,  Takeshi Miyazaki ,  Hidehito Takayama ,  Toshikazu Ohnishi

发明人： Matsuomi Nishimura ,  Kazumi Tanaka ,  Takeshi Miyazaki ,  Hidehito Takayama ,  Toshikazu Ohnishi

IPC分类号： G01N15/00 ,  G01N15/02 ,  G01N15/04 ,  G01N21/03 ,  G01N33/53 ,  G01N33/543 ,  G01N33/546

CPC分类号： G01N33/54366 ,  B01L3/5027 ,  G01N21/03 ,  G01N33/5304 ,  B01L2300/0825 ,  B01L2300/0887 ,  B01L2400/0406 ,  G01N15/0255 ,  G01N2015/0053 ,  G01N2015/0065 ,  G01N2015/0092 ,  G01N2015/045 ,  G01N2021/0346 ,  Y10S436/805 ,  Y10S436/807 ,  Y10S436/808 ,  Y10S436/809 ,  Y10S436/81 ,  Y10S436/824 ,  Y10S436/908

摘要： On a slab-like board formed of a transparent material is closely attached a wedge-shaped transparent cover member provided with a recess in a central inner portion, thereby to form a clearance. The height of the clearance between the recess and the board is configured to decrease continuously or in steps. When an immunological active substance such as a monoclonal antibody is caused to sensitize carrier particles F, and a reagent having the carrier particles F dispersed into a liquid medium mainly composed of the water is mixed with a specimen, the reaction will occur in which the flocculate is formed from plural carrier particles. When this reaction liquid is poured into the clearance through the opening, the reaction liquid penetrates in the direction having a narrower vertical spacing due to surface tension. A single carrier particle unflocculated can move deep within the recess because it is small in diameter, but the flocculate G is trapped on its way and can not move because of its size.

摘要翻译： 在由透明材料形成的板状板上紧密地连接有在中心内部设置有凹部的楔形透明盖构件，从而形成间隙。 凹槽和板之间的间隙的高度被配置为连续或逐步减小。 当使诸如单克隆抗体的免疫活性物质使载体颗粒F致敏时，将分散在主要由水组成的液体介质中的载体颗粒F的试剂与样品混合时，将发生絮凝反应 由多个载体颗粒形成。 当该反应液通过开口倒入间隙中时，由于表面张力，反应液体沿着垂直间隔较窄的方向渗透。 未絮凝的单载体颗粒由于其直径小而在凹槽内深深移动，而是絮凝的G被捕获，因为其尺寸不能移动。

公开(公告)号：US5380490A

公开(公告)日：1995-01-10

申请号：US821049

申请日：1992-01-16

申请人： Hiroaki Hoshi ,  Matsuomi Nishimura ,  Kazumi Tanaka ,  Takeshi Miyazaki ,  Toshikazu Ohnishi ,  Hidehito Takayama

发明人： Hiroaki Hoshi ,  Matsuomi Nishimura ,  Kazumi Tanaka ,  Takeshi Miyazaki ,  Toshikazu Ohnishi ,  Hidehito Takayama

IPC分类号： G01N21/59 ,  G01N21/82 ,  G01N33/53 ,  G01N33/543

CPC分类号： G01N33/5438 ,  G01N21/82 ,  G01N33/5304 ,  G01N33/54313 ,  G01N2021/5953 ,  G01N2021/5957

摘要： An apparatus is so constructed as to measure a specific substance in a test specimen by the degree of the aggregation of carrier particles in a reaction solution in which the test specimen is mixed with the carrier particles which carry a substance specifically binding to the specific substance. The apparatus includes a substrate having comb-shaped electrodes mounting the reaction solution and amplifier and alternating oscillator for displaying a variable voltage to the comb-shaped electrodes. The test specimen measuring apparatus having the structure accelerates the aggregation of the reaction solution by applying an alternating voltate to the comb-shaped electrodes in a state that the reaction solution is being mounted on the substrate, and detects the spatial spectrum of the comb-shaped electrodes on which the reaction solution is mounted, thus performing the qualitative or quantitative detection of the presence of the substance in the test specimen.

摘要翻译： 一种装置被构造成通过载体颗粒在其中将试样与携带特异性结合物质的物质的载体颗粒混合的反应溶液中聚集的程度来测量试样中的特定物质。 该装置包括具有安装反应溶液的梳形电极的基板和用于向梳状电极显示可变电压的放大器和交替振荡器。 具有这种结构的试样测量装置在将反应溶液安装在基板上的状态下通过向梳状电极施加交流电压来加速反应溶液的聚集，并且检测梳形电极的空间光谱 在其上安装反应溶液的电极，从而进行定性或定量检测试样中物质的存在。

公开(公告)号：US5788819A

公开(公告)日：1998-08-04

申请号：US926009

申请日：1997-09-09

申请人： Toshikazu Onishi ,  Matsuomi Nishimura ,  Kazuo Isaka ,  Kazumi Tanaka ,  Takeshi Miyazaki

发明人： Toshikazu Onishi ,  Matsuomi Nishimura ,  Kazuo Isaka ,  Kazumi Tanaka ,  Takeshi Miyazaki

IPC分类号： B01F13/00 ,  H02K44/04 ,  H02K44/02 ,  G01N35/08

CPC分类号： B01F13/0001 ,  H02K44/04

摘要： A method for driving an ionic conductive liquid comprises placing a pair of electrodes in the ionic conductive liquid, and exerting an electromagnetic force to the liquid by application of a voltage between the pair of the electrodes to drive the liquid. A method for mixing and agitating a medium comprises feeding the medium to an ionic conductive liquid, placing a pair of electrodes in the ionic conductive liquid, and exerting an electromagnetic force to the liquid by application of a voltage to drive the liquid and to mix and agitate the medium. An apparatus for delivering a liquid comprises a container for housing an ionic conductive liquid, at least one pair of electrodes in the container, and a power source for applying an voltage between the pair of electrodes, the apparatus delivering the liquid from the container by driving the liquid according to the method for driving.

摘要翻译： 用于驱动离子导电液体的方法包括将一对电极放置在离子导电液体中，并且通过在一对电极之间施加电压来驱动液体，向液体施加电磁力。 用于混合和搅拌介质的方法包括将介质馈送到离子导电液体，将一对电极放置在离子导电液体中，并通过施加电压以驱动液体并混合和施加电磁力给液体， 搅动介质。 用于输送液体的装置包括用于容纳离子导电液体的容器，容器中的至少一对电极和用于在一对电极之间施加电压的电源，该装置通过驱动从容器输送液体 液体按照驾驶方法。

公开(公告)号：US5534441A

公开(公告)日：1996-07-09

申请号：US372870

申请日：1995-01-13

申请人： Takeshi Miyazaki ,  Kazumi Tanaka ,  Masanori Sakuranaga ,  Tadashi Okamoto

发明人： Takeshi Miyazaki ,  Kazumi Tanaka ,  Masanori Sakuranaga ,  Tadashi Okamoto

IPC分类号： G01N21/82 ,  G01N33/543 ,  G01N35/00 ,  G01N35/10 ,  G01N33/557

CPC分类号： G01N35/00732 ,  G01N21/82 ,  G01N33/54313 ,  G01N33/54366 ,  G01N2035/00524 ,  G01N2035/00554 ,  G01N2035/00564 ,  G01N2035/00752 ,  G01N2035/1032 ,  Y10S436/805 ,  Y10S436/813 ,  Y10S436/814

摘要： There are provided a method and an apparatus for measuring an immunologically active material by physically or chemically immobilizing a material being immunologically active to a material to be measured of a specimen onto dehydrated solid fine particles, providing a desired dispersed body of said immunologically active material immobilized onto said solid fine particles in a dispersing medium, adding the specimen to said dispersed body while stirring to react the specimen with the immunologically active material, thereby causing a reaction mixture in an agglutinated state and optically measuring said agglutinated state of the reaction mixture to thereby quantitatively determine the content of the material to be measured with an improved accuracy.

摘要翻译： 提供了一种用于通过物理地或化学地将待检测物质的免疫活性物质固定在脱水固体细颗粒上的方法和装置，从而提供所需的所述免疫活性物质分散体固定化 在分散介质中的所述固体微粒上，在搅拌下将样品加入到分散体中，使样品与免疫活性物质反应，从而使反应混合物呈凝集状态，并光学测量反应混合物的凝集态 以提高的准确度定量地测定待测材料的含量。

公开(公告)号：US5679581A

公开(公告)日：1997-10-21

申请号：US372877

申请日：1995-01-13

申请人： Takeshi Miyazaki ,  Kazumi Tanaka ,  Masanori Sakuranaga ,  Tadashi Okamoto

发明人： Takeshi Miyazaki ,  Kazumi Tanaka ,  Masanori Sakuranaga ,  Tadashi Okamoto

IPC分类号： G01N33/543 ,  G01N15/02 ,  G01N21/82 ,  G01N33/546 ,  G01N33/557

CPC分类号： G01N33/54313 ,  G01N21/82 ,  G01N15/0205 ,  Y10S436/805 ,  Y10S436/813 ,  Y10S436/814

摘要： There are provided a method and an apparatus for measuring an immunologically active material by physically or chemically immobilizing material immunologically active to a material to be measured of a specimen to the dehydrated solid fine particles, providing a desirable dispersion comprising said immunologically active material immobilized to said solid fine particles and said specimen in a liquid medium, reacting them to cause a reaction mixture in an agglutinated state and optically measuring said agglutinated state of the reaction mixture to thereby quantitatively determine the content of said material to be measured with an improved accuracy.

摘要翻译： 提供了一种用于通过物理或化学固定待测材料对于脱水固体细颗粒具有免疫学活性的材料来测量免疫活性材料的方法和装置，提供了包含固定在所述脱水固体微粒上的所述免疫活性材料的理想分散体 将固体微粒和所述样品置于液体介质中，使其反应产生凝集状态的反应混合物，并光学测量反应混合物的凝集态，从而以更高的精度定量测定待测量材料的含量。

公开(公告)号：US06224732B1

公开(公告)日：2001-05-01

申请号：US08734971

申请日：1996-11-19

申请人： Totaro Imasaka ,  Kazuo Isaka ,  Toshikazu Ohnishi ,  Takeshi Miyazaki

发明人： Totaro Imasaka ,  Kazuo Isaka ,  Toshikazu Ohnishi ,  Takeshi Miyazaki

IPC分类号： C25B900

CPC分类号： G01N30/6078 ,  G01N30/00 ,  G01N30/02 ,  G01N30/6026 ,  G01N30/6052 ,  G01N30/6082 ,  G01N30/6095 ,  G01N2030/285 ,  H05H3/04 ,  B01D2015/3895

摘要： A dispersing medium containing plural types of particles is let to flow in a flow path formed in a flow cell. The flow cell is irradiated with interfering light to form interference fringes of a pattern of stripes. In another arrangement, light is two-dimensionally scanned at high speed so as to cross a flow in the flow path. The moving particles receive a braking force by the light gradient force whenever they pass through each irradiation light stripe. In that case, greater braking forces act on particles having larger sizes (or larger refractive indices) than on particles having smaller sizes (or smaller refractive indices). Accordingly, the particles receiving smaller braking forces pass through the irradiated position more rapidly, so that particles can be separated to flow in the order from the particles receiving smaller braking forces to the particles receiving greater braking forces.

摘要翻译： 使含有多种粒子的分散介质在形成于流通池中的流路中流动。 用干涉光照射流动池，以形成条纹图案的干涉条纹。 在另一种布置中，光以高速二维地扫描，以便穿过流动路径中的流动。 每当它们通过每个照射光条纹时，移动的颗粒通过光梯度力接收制动力。 在这种情况下，与具有较小尺寸（或较小折射率）的颗粒相比，较大的制动力作用于具有较大尺寸（或更大折射率）的颗粒。 因此，接收较小制动力的颗粒更快地穿过照射位置，使得颗粒可以分离成从接收较小制动力的颗粒到接收更大制动力的颗粒的顺序流动。