摘要:
The present invention relates to droplet-based particle sorting. According to one embodiment, a droplet microactuator is provided and includes: (a) a suspension of particles; and (b) electrodes arranged for conducting droplet operations using droplets comprising particles. A method of transporting a particle is also provided, wherein the method includes providing a droplet comprising the particle and transporting the droplet on a droplet microactuator.
摘要:
Methods, systems and computer program products for controlling scheduling in an ATM node in a network are provided which aggregate connection information for connections to the ATM node to provide a set of aggregate connection information for the ATM node. The set of aggregate connection information is distributed to other ATM nodes in the network and may be used in accepting connections to the ATM network based on the aggregate connection information. Scheduling may be provided by associating a timestamp value in each cell associated with an accepted connection as it is received by the ATM node. Received cells are grouped into a plurality of cell groups in timestamp order based upon characteristics of the cells and cells are selected from each group so as to transmit the cells in earliest deadline first order.
摘要:
Beginning with a CAD specification of an object, the method and apparatus of the invention reduces the overall time to produce a shell of the object with a rapid prototyping machine. The reduction in time is achieved by interleaving the computation and the building tasks and by not requiring an explicit evaluation of a shell of the solid at the outset. The method is shown to operate with STL data, or with other CAD data, directly, and can also be employed to create non-planar objects, such as models of sheet metal parts that are represented only as surfaces. Furthermore, the method can be employed to produce a smooth outer surface even when employing polyhedral approximations to the surfaces of the object.
摘要:
The present invention is directed to a droplet actuator and methods of making and using the droplet actuator including one or more substrates configured to form a droplet operations gap and including a physical or chemical feature that may be provided at a predetermined locus within or exposed to the droplet operations gap and configured to retain a bubble in position within the droplet operations gap.
摘要:
A method of mixing a droplet, the method comprising providing a droplet on a surface, forming the droplet into a first “U” shape having a bottom region and two terminal ends, and simultaneously merging the terminal ends and splitting the droplet at the bottom region to form a second “U” shape which is substantially opposite the first “U” shape.
摘要:
Consistent with embodiments of the present invention, a method may be provided comprising receiving a search string corresponding to a desired node comprising a target parameter, a policy parameter, and a class parameter. The target parameter may be referenced with a target index table to determine which interfaces to search. The policy parameter may be referenced with a policy index table to determine a node-id of a policy node corresponding to the policy parameter. A level for the desired node may be determined based on the node-id. The class parameter may be referenced with the determined node-id with a class index table to access a bucket location. The desired node may then be searched for with the determined node-id at the determined level.
摘要:
The present invention provides droplet actuators, modified fluids and methods relating to droplet operations. An aspect includes a droplet actuator including a droplet operations substrate; an oil based filler fluid on the droplet operations substrate comprising an oil soluble additive in the filler fluid; and a droplet in contact with the oil based filler fluid. Still other aspects are provided.
摘要:
The invention provides droplet actuators with droplet operations surfaces for manipulating droplets, e.g., by conducting droplet operations. The droplet operations surfaces are typically exposed to a droplet operations gap. One or more regions of a droplet operation surface may include patterned topographic features. The invention also provides a droplet actuator in which one or both gap-facing droplet operations surfaces is formed using a removable film. The removable film may, in various embodiments, also include other components ordinarily associated with the droplet actuator substrate, such as the dielectric layer and the electrodes. Further, the invention provides droplet actuator devices and methods for coupling and/or sealing substrates of a droplet actuator, such as techniques for self-aligning assembly of droplet actuator substrates. The invention provides droplet actuators and methods of disassembling the droplet actuator in order to provide access for cleaning and/or recycling of droplet actuator surfaces.
摘要:
The invention provides droplet actuators and droplet actuator techniques. Among other things, the droplet actuators and methods are useful for manipulating beads on a droplet actuator, such as conducting droplet operations using bead-containing droplets on a droplet actuator. For example, beads may be manipulated on a droplet actuator in the context of executing a sample preparation protocol and/or an assay protocol. An output of the methods of the invention may be beads prepared for execution of an assay protocol. Another output of the methods of the invention may be results of an assay protocol executed using beads. Among the methods described herein are methods of concentrating beads in droplets, methods of washing beads, methods of suspending beads, methods of separating beads, methods of localizing beads within a droplet, methods of forming emulsions in which droplets include beads, methods of loading beads into a droplet operations gap of a droplet actuator, methods of organizing beads in a monolayer, and methods of capturing, trapping or restraining beads.
摘要:
The invention provides a droplet actuator comprising a base substrate and a top substrate separated to form a gap, wherein: (a) the base substrate comprises electrodes configured for conducting droplet operations in the gap; and (b) the top substrate comprises a first portion coupled to second portion, where the second portion comprises one or more openings establishing a fluid path extending from an exterior of the droplet actuator and into the gap. The invention also provides related methods of manufacturing the droplet actuator, methods of using the droplet actuator, and methods of loading the droplet actuator.