公开(公告)号：US09809848B2

公开(公告)日：2017-11-07

申请号：US14775960

申请日：2014-03-14

Applicant: Fluidigm Corporation

Inventor： Robert C. Jones

IPC: C12Q1/68 ,  G01N33/53 ,  G01N33/532

CPC classification number: C12Q1/686 ,  C12Q1/682 ,  G01N33/532 ,  G01N2458/10 ,  C12Q2521/101 ,  C12Q2531/113 ,  C12Q2537/125 ,  C12Q2545/114

Abstract: Methods and reagents for detection and analysis of nucleic acids are provided. The methods employ proximity extension assays for detection of a target nucleic acids of interest, e.g., a target RNA. The method can additionally be used in multiplex assays with a protein proximity extension assay to detect protein.

公开(公告)号：US09677119B2

公开(公告)日：2017-06-13

申请号：US14180262

申请日：2014-02-13

Applicant: Fluidigm Corporation

Inventor： Andrew May ,  Peilin Chen ,  Jun Wang ,  Fiona Kaper ,  Megan Anderson

IPC: C12Q1/68 ,  C07H21/02 ,  B01L3/00 ,  B01L7/00

CPC classification number: C12Q1/6806 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/52 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/0655 ,  C12Q1/686 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2527/143 ,  C12Q2535/122 ,  C12Q2549/119 ,  C12Q2563/179 ,  C12Q2565/629

Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.

公开(公告)号：US09657344B2

公开(公告)日：2017-05-23

申请号：US14663010

申请日：2015-03-19

Applicant: FLUIDIGM CORPORATION

Inventor： Stanley N. Lapidus ,  Philip R. Buzby ,  Timothy D. Harris

IPC: C12Q1/68 ,  C07H21/00

CPC classification number: C12Q1/6874 ,  C12Q1/6869 ,  C12Q2527/113

Abstract: The invention provides methods for sequencing a polynucleotide comprising stopping an extension cycle in a sequence by synthesis reaction before the reaction has run to near or full completion.

公开(公告)号：US09487822B2

公开(公告)日：2016-11-08

申请号：US12170414

申请日：2008-07-09

Applicant: Simant Dube ,  Jian Qin ,  Ramesh Ramakrishnan

Inventor： Simant Dube ,  Jian Qin ,  Ramesh Ramakrishnan

IPC: G01N33/483 ,  C12Q1/68 ,  G01N33/00 ,  B01L3/00 ,  G01N35/00

CPC classification number: C12Q1/6837 ,  B01L3/5027 ,  G01N35/00594 ,  Y10T436/143333 ,  C12Q2527/137 ,  C12Q2537/16 ,  C12Q2537/165

Abstract: A method of estimating a concentration of DNA molecules in a biological sample includes storing a number of a plurality of reaction sites in a memory and distributing the biological sample among the plurality of reaction sites. The method also includes determining a number of the plurality of reaction sites characterized by a presence of one or more of the DNA molecules and computing a portion of the plurality of reaction sites characterized by the presence of the one or more of the DNA molecules. The method further includes estimating the concentration of the DNA molecules as a function of the portion of the plurality of reaction sites and computing a confidence interval for the estimated concentration of DNA molecules.

Abstract translation: 估计生物样品中DNA分子浓度的方法包括将多个反应位点存储在存储器中并在多个反应位点之间分配生物样品。 该方法还包括确定多个反应位点的数目，其特征在于存在一个或多个DNA分子，并计算以一个或多个DNA分子存在为特征的多个反应位点的一部分。 该方法还包括估计DNA分子的浓度作为多个反应位点的一部分的函数，并计算DNA分子的估计浓度的置信区间。

公开(公告)号：US09429500B2

公开(公告)日：2016-08-30

申请号：US13781292

申请日：2013-02-28

Applicant: Fluidigm Corporation

Inventor： Brian Fowler ,  Jake Kimball ,  Myo Thu Maung ,  Andrew May ,  Michael C. Norris ,  Dominique G. Toppani ,  Marc A. Unger ,  Jing Wang ,  Jason A. A. West

IPC: G01N1/34 ,  G01N1/28 ,  C12P19/34 ,  C12Q1/68 ,  G01N15/14 ,  B01L3/00 ,  B01L7/00

CPC classification number: G01N1/28 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0864 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/086 ,  B01L2400/088 ,  C12P19/34 ,  C12Q1/6813 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N1/34 ,  G01N15/1484 ,  C12Q2565/629

Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.

公开(公告)号：US09364829B2

公开(公告)日：2016-06-14

申请号：US12945483

申请日：2010-11-12

Applicant: Christian A. Heid ,  Antoine Daridon

Inventor： Christian A. Heid ,  Antoine Daridon

IPC: B01L3/00 ,  C12Q1/68 ,  B01L7/00

CPC classification number: B01L3/5027 ,  B01L7/52 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2400/0487 ,  B01L2400/0655 ,  C12Q1/686 ,  C12Q2525/155 ,  C12Q2525/301

Abstract: The invention relates to methods, reagents and devices for detection and characterization of nucleic acids, cells, and other biological samples. Assay method are provided in which a sample is partitioned into sub-samples, and analysis of the contents of the sub-samples carried out. The invention also provides microfluidic devices for conducting the assay. The invention also provides an analysis method using a universal primers and probes for amplification and detection.

Abstract translation: 本发明涉及核酸，细胞和其他生物样品的检测和表征的方法，试剂和装置。 提供了将样品分割成子样品并进行子样品的内容的分析的测定方法。 本发明还提供用于进行测定的微流体装置。 本发明还提供了使用通用引物和探针进行扩增和检测的分析方法。

公开(公告)号：US09284605B2

公开(公告)日：2016-03-15

申请号：US14318104

申请日：2014-06-27

Applicant: Fluidigm Corporation

Inventor： Stanley N. Lapidus

IPC: C12Q1/68

CPC classification number: C12Q1/68 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2565/518 ,  C12Q2563/107 ,  C12Q2533/101

Abstract: The invention provides methods and devices for detecting, enumerating or identifying target nucleic acid molecules using immobilized capture probes and single molecule sequencing techniques.

Abstract translation: 本发明提供了使用固定的捕获探针和单分子测序技术来检测，列举或鉴定靶核酸分子的方法和装置。

公开(公告)号：US09234237B2

公开(公告)日：2016-01-12

申请号：US14547442

申请日：2014-11-19

Applicant: Fluidigm Corporation

Inventor： Marc A. Unger ,  Geoffrey Richard Facer ,  Barry Clerkson ,  Christopher G. Cesar ,  Neil Switz

IPC: G01N21/00 ,  G01N15/06 ,  C12Q1/68 ,  G01N21/64 ,  G01N27/447 ,  G02B21/16 ,  G01N21/84 ,  G01N21/75 ,  G02B21/36 ,  B01L3/00 ,  B01L7/00

CPC classification number: C12Q1/686 ,  B01J2219/00576 ,  B01J2219/00704 ,  B01L3/5027 ,  B01L7/52 ,  G01N21/64 ,  G01N21/6452 ,  G01N21/6456 ,  G01N21/6486 ,  G01N21/75 ,  G01N21/8483 ,  G01N27/44721 ,  G01N2021/6421 ,  G01N2201/061 ,  G02B21/16 ,  G02B21/36

Abstract: An apparatus for imaging one or more selected fluorescence indications from a microfluidic device. The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device. The imaging path provides for transmission of one or more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic device. The chamber has a chamber size, the chamber size being characterized by an actual spatial dimension normal to the imaging path. The apparatus also includes an optical lens system coupled to the imaging path. The optical lens system is adapted to transmit the one or more fluorescent signals associated with the chamber.

公开(公告)号：US09182322B2

公开(公告)日：2015-11-10

申请号：US14091344

申请日：2013-11-27

Applicant: Fluidigm Corporation

Inventor： Tim Woudenberg ,  Jing Wang ,  Hou-Pu Chou

IPC: G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  G01N1/28 ,  H04W76/06 ,  H04W4/00

CPC classification number: B01L3/502715 ,  B01F13/0059 ,  B01F13/0094 ,  B01F13/1013 ,  B01F13/1022 ,  B01J2219/00317 ,  B01J2219/00479 ,  B01J2219/00585 ,  B01L3/5025 ,  B01L3/502723 ,  B01L3/502738 ,  B01L3/502746 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2300/0829 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2300/1805 ,  B01L2400/0487 ,  B01L2400/0605 ,  B01L2400/0655 ,  B01L2400/082 ,  B33Y80/00 ,  G01N1/28 ,  H04W4/70 ,  H04W76/30

Abstract: Microfluidic devices are described that include a rigid base layer, and an elastomeric layer on the base layer. The elastomeric layer may include at least part of a fluid channel for transporting a liquid reagent, and a vent channel that accepts gas diffusing through the elastomeric layer from the flow channel and vents it out of the elastomeric layer. The devices may also include a mixing chamber fluidly connected to the fluid channel, and a control channel overlapping with a deflectable membrane that defines a portion of the flow channel, where the control channel may be operable to change a rate at which the liquid reagent flows through the fluid channel. The devices may further include a rigid plastic layer on the elastomeric layer.

Abstract translation: 描述了包括刚性基层和基层上的弹性体层的微流体装置。 弹性体层可以包括用于输送液体试剂的流体通道的至少一部分，以及排气通道，其接纳从流动通道穿过弹性体层扩散的气体并将其从弹性体层排出。 装置还可以包括流体连接到流体通道的混合室，以及与限定流动通道的一部分的可偏转膜重叠的控制通道，其中控制通道可操作以改变液体试剂流动的速率 通过流体通道。 所述装置还可包括弹性体层上的刚性塑料层。

公开(公告)号：US09163053B2

公开(公告)日：2015-10-20

申请号：US12631074

申请日：2009-12-04

Applicant: Suhaib Siddiqi ,  J. William Efcavitch ,  Judith Mitchell ,  Subramanian Marappan ,  Jayson Bowers ,  Mirna Jaroza

Inventor： Suhaib Siddiqi ,  J. William Efcavitch ,  Judith Mitchell ,  Subramanian Marappan ,  Jayson Bowers ,  Mirna Jaroza

IPC: C07H19/00 ,  C07H19/04 ,  C07H21/02 ,  C12Q1/68 ,  C12P19/34 ,  C07H19/06 ,  C07H19/16 ,  A61K49/00

CPC classification number: C12Q1/6876 ,  A61K49/0041 ,  A61K49/0052 ,  C07H19/06 ,  C07H19/16 ,  C12Q1/6869 ,  C12Q2563/107

Abstract: The invention generally relates to nucleotide analogs and methods of their use in sequencing-by-synthesis reactions. In certain embodiments, the invention provides a nucleotide analog including a detectable label attached to a nitrogenous base portion of a nucleotide analog by a cleavable linker, in which contact of the analog with at least one activating agent results in cleavage of the label and elimination of the linker, thereby producing a natural nucleotide, a 9-deaza-G, 9-deaza-A, or ψ-uridine.

Abstract translation: 本发明一般涉及核苷酸类似物及其在合成反应测序中的用途。 在某些实施方案中，本发明提供核苷酸类似物，其包括通过可切割接头连接到核苷酸类似物的含氮碱基部分的可检测标记，其中所述类似物与至少一种活化剂的接触导致标记的切割和消除 接头，从而产生天然核苷酸，9-脱氮-G，9-脱氮-A或ψ-尿苷。