Patent search ap:("Berkeley Lights Page Inc.") AND inv:"John A. Tenney"

11.

发明授权
Automated detection and repositioning of micro-objects in microfluidic devices 有权

公开(公告)号：US12272048B2

公开(公告)日：2025-04-08

申请号：US17062907

申请日：2020-10-05

Applicant: Berkeley Lights, Inc.

Inventor： Fenglei Du , Paul M. Lundquist , John A. Tenney , Troy A. Lionberger

IPC: G06T7/00 , B01L3/00 , G01N15/00 , G01N15/01 , G01N15/10 , G01N15/14 , G01N15/1433 , G01N15/1434 , G01N21/05 , G01N21/17 , G01N21/64 , G01N27/453 , G06T5/50

Abstract: Methods are provided for the automated detection of micro-objects in a microfluidic device. In addition, methods are provided for repositioning micro-objects in a microfluidic device. In addition, methods are provided for separating micro-objects in a spatial region of the microfluidic device.

12.

发明公开
Methods of Assaying a Biological Cell 审中-公开

公开(公告)号：US20230323433A1

公开(公告)日：2023-10-12

申请号：US18178927

申请日：2023-03-06

Applicant: Berkeley Lights, Inc.

Inventor： Matthew Asuka Kubit , Joshua David Mast , John Junyeon Kim , Alexander Gerald Olson , Preston Lock Ng , Arlvin Louis Ellefson , Shruthi Sreedhar Kubatur , Vincent Haw Tien Pai , Minha Park , Po-Yuan Tung , Jason C. Briggs , Patrick N. Ingram , Katrine Elise Dailey , Maryam Shansab , Jason M. McEwen , Adrienne T. Higa , Hongye Zhou , Zhen Hu , John A. Tenney

IPC: C12Q1/6834 , C12Q1/6844 , C12Q1/6804

CPC classification number: C12Q1/6834 , C12Q1/6804 , C12Q1/6844 , C12Q2600/16

Abstract: Disclosed herein are methods for performing assays, including general functional assays, on a biological cell. Also disclosed herein are methods of barcoding the 5′ ends of RNA from a biological cell and methods of preparation of expression constructs from the barcoded RNA. The barcoded RNA can encode proteins of interest, such as B cell receptor (BCR) heavy and light chain sequences. The expression constructs can be generated individually or in a paired/multiplexed manner, allowing rapid re-expression of individual proteins or protein complexes.

13.

发明公开
SYSTEMS AND METHODS FOR ANALYSES OF BIOLOGICAL SAMPLES 审中-公开

公开(公告)号：US20230282313A1

公开(公告)日：2023-09-07

申请号：US17971400

申请日：2022-10-21

Applicant: BERKELEY LIGHTS, INC.

Inventor： Darshan Thaker , Keith J. Breinlinger , Vincent Haw Tien Pai , Christoph Andreas Neyer , Thomas M. Vetterli , Hayley M. Bennett , Elisabeth Marie Walczak , Alexander Gerald Olson , Wesley Arthur Zink , John A. Tenney , Oleksandr Tokmakov , Igor Fastnacht , Yuriy Nicheporuk , Andriy Koval , Khrystyna Andres , Alona Kostenko

IPC: G16B40/20 , G16B45/00 , G16B30/20 , B01L3/00 , G01N15/14

CPC classification number: G16B40/20 , G16B45/00 , G16B30/20 , B01L3/502784 , G01N15/1463 , B01L2200/027

Abstract: Disclosed are methods, systems, and articles of manufacture for performing a process on biological samples. An analysis of biological samples in multiple regions of interest in a microfluidic device and a timeline correlated with the analysis may be identified. One or more region-of-interest types for the multiple regions of interest may be determined; and multiple characteristics may be determined for the biological samples based at least in part upon the one or more region-of-interest types. Associated data that respectively correspond to the multiple regions of interest in a user interface for at least a portion of the biological samples in the user interface based at least in part upon the multiple identifiers and the timeline. A count of the biological samples in a region of interest may be determined based at least in part upon a class or type of data using a convolutional neural network (CNN).

14.

发明授权
Light sequencing and patterns for dielectrophoretic transport 有权

公开(公告)号：US11376591B2

公开(公告)日：2022-07-05

申请号：US16893885

申请日：2020-06-05

Applicant: Berkeley Lights, Inc.

Inventor： Troy A. Lionberger , Brandon R. Bruhn , John A. Tenney , Eric D. Hobbs

IPC: B01L3/00 , G01N27/447 , B03C5/00 , B03C5/02

Abstract: Optically-actuated microfluidic devices permit the use of spatially-modulated light to manipulate micro-objects such as biological cells. Systems and methods are described for providing sequences of light patterns to move and direct a plurality of micro-objects within the environment of a microfluidic device. The sequenced light patterns provide improved efficiency in directing the transport of the plurality of micro-objects. Other embodiments are described.

15.

发明授权
Methods, systems and kits for in-pen assays 有权

公开(公告)号：US11203018B2

公开(公告)日：2021-12-21

申请号：US16160816

申请日：2018-10-15

Applicant: Berkeley Lights, Inc.

Inventor： Troy A. Lionberger , Matthew E. Fowler , Phillip J. M. Elms , Kevin D. Loutherback , Randall D. Lowe, Jr. , Jian Gong , J. Tanner Nevill , Gang F. Wang , Gregory G. Lavieu , John A. Tenney , Aathavan Karunakaran , Anupam Singhal , I-Jong Lin

IPC: B01L3/00 , G01N21/64 , B03C5/02 , B03C5/00 , B01L9/00 , G06T7/00 , G01N21/05

Abstract: Methods, systems and kits are described herein for detecting the results of an assay. In particular, the methods, systems and devices of the present disclosure rely on a difference between the diffusion rates of a reporter molecule and an analyte of interest in order to quantify an amount of analyte in a microfluidic device. The analyte may be a secreted product of a biological micro-object.

16.

发明申请
SYSTEMS AND METHODS FOR ANALYSES OF BIOLOGICAL SAMPLES 有权

公开(公告)号：US20210272654A1

公开(公告)日：2021-09-02

申请号：US17325196

申请日：2021-05-19

Applicant: BERKELEY LIGHTS, INC.

Inventor： Darshan Thaker , Keith J. Breinlinger , Vincent Haw Tien Pai , Christoph Andreas Neyer , Thomas M. Vetterli , Hayley M. Bennett , Elisabeth Marie Walczak , Alexander Gerald Olson , Wesley Arthur Zink , John A. Tenney , Oleksandr Tokmakov , Igor Fastnacht , Yuriy Nicheporuk , Andriy Koval , Khrystyna Andres , Alona Kostenko

IPC: G16B40/20 , G16B30/20 , G16B45/00 , B01L3/00

Abstract: Disclosed are methods, systems, and articles of manufacture for performing a process on biological samples. An analysis of biological samples in multiple regions of interest in a microfluidic device and a timeline correlated with the analysis may be identified. One or more region-of-interest types for the multiple regions of interest may be determined; and multiple characteristics may be determined for the biological samples based at least in part upon the one or more region-of-interest types. Associated data that respectively correspond to the multiple regions of interest in a user interface for at least a portion of the biological samples in the user interface based at least in part upon the multiple identifiers and the timeline. A count of the biological samples in a region of interest may be determined based at least in part upon a class or type of data using a convolutional neural network (CNN).

17.

发明授权
Light sequencing and patterns for dielectrophoretic transport 有权

公开(公告)号：US10675625B2

公开(公告)日：2020-06-09

申请号：US15487795

申请日：2017-04-14

Applicant: Berkeley Lights, Inc.

Inventor： Troy A. Lionberger , Brandon R. Bruhn , John A. Tenney , Eric D. Hobbs

IPC: B01L3/00 , G01N27/447 , B03C5/00 , B03C5/02

Abstract: Optically-actuated microfluidic devices permit the use of spatially-modulated light to manipulate micro-objects such as biological cells. Systems and methods are described for providing sequences of light patterns to move and direct a plurality of micro-objects within the environment of a microfluidic device. The sequenced light patterns provide improved efficiency in directing the transport of the plurality of micro-objects. Other embodiments are described.