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    Methods of Improving Accuracy and Precision of Droplet Metering Using an On-Actuator Reservoir as the Fluid Input
    13.
    发明申请
    Methods of Improving Accuracy and Precision of Droplet Metering Using an On-Actuator Reservoir as the Fluid Input 审中-公开
    使用致动器水库作为流体输入提高液滴计量精度和精度的方法

    公开(公告)号：US20160175859A1

    公开(公告)日：2016-06-23

    申请号：US14911191

    申请日：2014-08-13

    申请人： ADVANCED LIQUID LOGIC, INC.

    发明人： Uichong B. Yi ,  Vijay Srinivasan

    IPC分类号： B05B5/025 ,  B05B12/08

    CPC分类号： B05B5/0255 ,  B05B12/082 ,  G01F23/22 ,  G01F23/24 ,  G01F23/26

    摘要： The present invention is directed to methods of improving accuracy of droplet metering using at least one on-actuator reservoir as the fluid input. In some embodiments, the on-actuator reservoir that is used for metering droplets includes a loading port, a liquid storage zone, a droplet metering zone, and a droplet dispensing zone. The on-actuator reservoirs are designed to prevent liquid flow-back into the loading port and to prevent liquid from flooding into the droplet operations gap in the dispensing zone.

    摘要翻译： 本发明涉及使用至少一个致动器容器作为流体输入来提高液滴计量精度的方法。 在一些实施例中，用于计量液滴的致动器储存器包括装载端口，液体存储区域，液滴计量区域和液滴分配区域。 致动器容器被设计成防止液体回流到装载口中并防止液体溢出到分配区域中的液滴操作间隙中。

    Methods of On-Actuator Temperature Measurement
    14.
    发明申请
    Methods of On-Actuator Temperature Measurement 审中-公开
    执行机构温度测量方法

    公开(公告)号：US20160161343A1

    公开(公告)日：2016-06-09

    申请号：US14905679

    申请日：2014-07-21

    申请人： ADVANCED LIQUID LOGIC, INC.

    发明人： Gregory SMITH ,  Randall L. LUCK

    IPC分类号： G01K7/16 ,  G01K13/00

    CPC分类号： G01K7/16 ,  B01L3/502792 ,  B01L2200/147 ,  B01L2300/0645 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0409 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0433 ,  B01L2400/0442 ,  B01L2400/0457 ,  B01L2400/0478 ,  G01K13/00

    摘要： The present invention provides methods for on-actuator temperature measurement and temperature control, including where one or more of the temperature sensors are combined with one or more heaters that are formed of wiring traces and/or providing heaters designed for one-to-one correspondence to the temperature sensors to form temperature sensor-heater pairs. The present invention also provides methods for on-actuator temperature measurement and temperature control in which the temperature sensors comprise a connection comprising a plurality of terminals by which an amount of current can be applied and then a voltage measured, wherein the voltage that is measured across the temperature sensors can be accurately correlated to a temperature.

    摘要翻译： 本发明提供了用于致动器温度测量和温度控制的方法，包括其中一个或多个温度传感器与由布线迹线形成的一个或多个加热器组合和/或提供设计用于一对一对应的加热器 到温度传感器形成温度传感器加热器对。 本发明还提供了用于致动器温度测量和温度控制的方法，其中温度传感器包括包括多个端子的连接，通过该连接可以施加电流量，然后测量电压，其中测量的电压 温度传感器可以准确地与温度相关。

    Droplet actuators that include molecular barrier coatings
    16.
    发明授权
    Droplet actuators that include molecular barrier coatings 有权
    液滴执行器，包括分子屏障涂层

    公开(公告)号：US09223317B2

    公开(公告)日：2015-12-29

    申请号：US13915842

    申请日：2013-06-12

    申请人： Advanced Liquid Logic Inc.

    发明人： Theodore Winger

    IPC分类号： G05D7/06 ,  B01L3/00 ,  B01L7/00

    CPC分类号： G05D7/0629 ,  B01L3/0268 ,  B01L3/502707 ,  B01L3/502784 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0681 ,  B01L2300/0887 ,  B01L2300/12 ,  B01L2300/16 ,  B01L2300/161 ,  B01L2300/165 ,  B01L2400/0427 ,  B01L2400/043 ,  Y10T137/0318 ,  Y10T137/206

    摘要： Droplet actuators that include molecular barrier coatings are provided. The molecular barrier coating may be provided atop the conductive layer of the top substrate, atop the droplet operations electrodes of the bottom substrate, or both. Where the conductive layer of the top substrate and/or the droplet operations electrodes of the bottom substrate are formed of an electrically conductive organic polymer, such as poly(3,4-ethylenedioxythiophene) poly(styrenesulfonate) (PEDOT:PSS), the molecular barrier coating helps to immobilize the contents of the PEDOT:PSS layer. Further, the molecular barrier coating reduces, preferably entirely eliminates, moisture from seeping into the electrically conducting organic polymer. Methods of conducting droplet operations using the disclosed droplet actuators are also provided.

    摘要翻译： 提供了包括分子阻隔涂层的液滴致动器。 分子屏障涂层可以设置在顶部基底的导电层顶部，位于底部基底的液滴操作电极顶部，或两者之上。 在顶部基板的导电层和/或底部基板的液滴操作电极由诸如聚（3,4-亚乙基二氧噻吩）聚（苯乙烯磺酸盐）（PEDOT：PSS）的导电有机聚合物形成的情况下，分子 阻隔涂层有助于固定PEDOT：PSS层的内容物。 此外，分子阻隔涂层减少，优选完全消除水分渗透到导电有机聚合物中。 还提供了使用所公开的液滴致动器进行液滴操作的方法。

    Integrated Droplet Actuator for Gel Electrophoresis and Molecular Analysis
    17.
    发明申请
    Integrated Droplet Actuator for Gel Electrophoresis and Molecular Analysis 有权
    用于凝胶电泳和分子分析的集成滴液致动器

    公开(公告)号：US20150323496A1

    公开(公告)日：2015-11-12

    申请号：US14795304

    申请日：2015-07-09

    申请人： Advanced Liquid Logic, Inc. ,  Duke University

    发明人： Michael G. Pollack ,  Vijay Srinivasan ,  Zhishan Hua ,  Hon Lung Chu ,  Michael Hauser ,  Allen Eckhardt

    IPC分类号： G01N27/447 ,  B01L3/00

    CPC分类号： G01N27/44743 ,  B01L3/502715 ,  B01L3/502792 ,  B01L7/525 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2300/088 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2400/0406 ,  B01L2400/043 ,  B01L2400/0457 ,  B01L2400/046 ,  G01N27/44791

    摘要： The invention is directed to a droplet actuator device and methods for integrating gel electrophoresis analysis with pre or post-analytical sample handling as well as other molecular analysis processes. Using digital microfluidics technology, the droplet actuator device and methods of the invention provide the ability to perform gel electrophoresis and liquid handling operations on a single integrated device. The integrated liquid handling operations may be used to prepare and deliver samples to the electrophoresis gel, capture and subsequently process products of the electrophoresis gel or perform additional assays on the same sample materials which are analyzed by gel electrophoresis. In one embodiment, one or more molecular assays, such as nucleic acid (e.g., DNA) quantification by real-time PCR, and one or more sample processing operations such as sample dilution is performed on a droplet actuator integrated with an electrophoresis gel. In one embodiment, an electrophoresis gel may be integrated on the top substrate of the droplet actuator.

    摘要翻译： 本发明涉及液滴致动器装置以及用于将凝胶电泳分析与分析前样品或分析后处理以及其它分子分析方法相集成的方法。 使用数字微流体技术，液滴致动器装置和本发明的方法提供了在单个集成装置上执行凝胶电泳和液体处理操作的能力。 集成的液体处理操作可以用于制备并将样品递送到电泳凝胶，捕获并随后处理电泳凝胶的产物，或在通过凝胶电泳分析的相同样品材料上进行附加测定。 在一个实施方案中，在与电泳凝胶结合的液滴致动器上进行一个或多个分子测定，例如通过实时PCR的核酸（例如DNA）定量和一个或多个样品处理操作，例如样品稀释。 在一个实施方案中，电泳凝胶可以集成在液滴致动器的顶部基底上。

    Integrated droplet actuator for gel; electrophoresis and molecular analysis
    18.
    发明授权
    Integrated droplet actuator for gel; electrophoresis and molecular analysis 有权
    凝胶液滴致动器 电泳和分子分析

    公开(公告)号：US09091649B2

    公开(公告)日：2015-07-28

    申请号：US13508300

    申请日：2010-11-08

    申请人： Michael G. Pollack ,  Vijay Srinivasan ,  Zhishan Hua ,  Hon Lung Chu ,  Michael Hauser ,  Allen Eckhardt

    发明人： Michael G. Pollack ,  Vijay Srinivasan ,  Zhishan Hua ,  Hon Lung Chu ,  Michael Hauser ,  Allen Eckhardt

    IPC分类号： G01N27/447 ,  B01L7/00 ,  B01L3/00

    CPC分类号： G01N27/44743 ,  B01L3/502715 ,  B01L3/502792 ,  B01L7/525 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2300/088 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2400/0406 ,  B01L2400/043 ,  B01L2400/0457 ,  B01L2400/046 ,  G01N27/44791

    摘要： The invention is directed to a droplet actuator device and methods for integrating gel electrophoresis analysis with pre or post-analytical sample handling as well as other molecular analysis processes. Using digital microfluidics technology, the droplet actuator device and methods of the invention provide the ability to perform gel electrophoresis and liquid handling operations on a single integrated device. The integrated liquid handling operations may be used to prepare and deliver samples to the electrophoresis gel, capture and subsequently process products of the electrophoresis gel or perform additional assays on the same sample materials which are analyzed by gel electrophoresis. In one embodiment, one or more molecular assays, such as nucleic acid (e.g., DNA) quantification by real-time PCR, and one or more sample processing operations such as sample dilution is performed on a droplet actuator integrated with an electrophoresis gel. In one embodiment, an electrophoresis gel may be integrated on the top substrate of the droplet actuator.

    摘要翻译： 本发明涉及液滴致动器装置以及用于将凝胶电泳分析与分析前样品或分析后处理以及其它分子分析方法相集成的方法。 使用数字微流体技术，液滴致动器装置和本发明的方法提供了在单个集成装置上执行凝胶电泳和液体处理操作的能力。 集成的液体处理操作可以用于制备并将样品递送到电泳凝胶，捕获并随后处理电泳凝胶的产物，或在通过凝胶电泳分析的相同样品材料上进行附加测定。 在一个实施方案中，在与电泳凝胶结合的液滴致动器上进行一个或多个分子测定，例如通过实时PCR的核酸（例如DNA）定量和一个或多个样品处理操作，例如样品稀释。 在一个实施方案中，电泳凝胶可以集成在液滴致动器的顶部基底上。

    Bead manipulation techniques
    19.
    发明授权
    Bead manipulation techniques 有权
    珠操纵技术

    公开(公告)号：US09050606B2

    公开(公告)日：2015-06-09

    申请号：US14491231

    申请日：2014-09-19

    申请人： ADVANCED LIQUID LOGIC, INC.

    发明人： Vamsee K. Pamula ,  Arjun Sudarsan ,  Ramakrishna Sista

    IPC分类号： G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  B03C5/00 ,  G01N33/543 ,  B03C1/01 ,  B03C1/28 ,  B03C5/02

    CPC分类号： B03C7/026 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0887 ,  B01L2400/0424 ,  B01L2400/0427 ,  B03C1/01 ,  B03C1/288 ,  B03C5/00 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  G01N33/54326

    摘要： The invention provides a method of redistributing magnetically responsive beads in a droplet. The method may include conducting on a droplet operations surface one or more droplet operations using the droplet without removing the magnetically responsive beads from the region of the magnetic field. The droplet operations may in some cases be electrode-mediated. The droplet operations may redistribute and/or circulate the magnetically responsive beads within the droplet. In some cases, the droplet may include a sample droplet may include a target analyte. The redistributing of the magnetically responsive beads may cause target analyte to bind to the magnetically responsive beads. In some cases, the droplet may include unbound substances in a wash buffer. The redistributing of the magnetically responsive beads causes unbound substances to be freed from interstices of an aggregated set or subset of the magnetically responsive beads.

    摘要翻译： 本发明提供了一种在液滴中重新分布磁响应珠的方法。 该方法可以包括在液滴操作表面上使用液滴进行一个或多个液滴操作，而不从磁场的区域移除磁响应珠。 在某些情况下，液滴操作可能是电极介导的。 液滴操作可以在液滴内重新分布和/或循环磁响应珠。 在一些情况下，液滴可以包括样品液滴可以包括靶分析物。 磁响应珠的重新分布可能导致目标分析物结合磁响应珠。 在一些情况下，液滴可以包括洗涤缓冲液中的未结合物质。 磁响应珠子的重新分布导致未结合物质从磁性响应珠粒的聚集或子集的空隙中释放出来。

    Droplet Dispensing Device and Methods
    20.
    发明申请
    Droplet Dispensing Device and Methods 审中-公开
    滴液分配装置及方法

    公开(公告)号：US20150075985A1

    公开(公告)日：2015-03-19

    申请号：US14541825

    申请日：2014-11-14

    申请人： ADVANCED LIQUID LOGIC, INC.

    发明人： Michael G. Pollack ,  Vamsee K. Pamula ,  Vijay Srinivasan

    IPC分类号： B01L3/00 ,  G01N27/447

    CPC分类号： B01L3/502761 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502792 ,  B01L2200/0605 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/16 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/088 ,  B01L2300/089 ,  B01L2400/0427 ,  B01L2400/043 ,  G01N27/447

    摘要： The invention provides nonlimiting examples of structures for and methods of dispensing droplets in a droplet actuator. The droplet actuator structures and methods of the invention exhibit numerous advantages over droplet actuators of the prior art. In various embodiments, the structures and methods of the invention provide, among other things, improved efficiency, throughput, scalability, and/or droplet uniformity, as compared with existing droplet actuators. Further, in some embodiments, the droplet actuators provide configurations for improved methods of loading and/or unloading fluid and/or droplets. In yet other embodiments, the droplet actuators provide fluid loading configurations for loading numerous fluid reservoirs in a substantially simultaneous and/or substantially sequential manner.

    摘要翻译： 本发明提供了用于在液滴致动器中分配液滴的结构的方法和方法的非限制性实例。 本发明的液滴致动器结构和方法与现有技术的液滴致动器相比表现出许多优点。 在各种实施例中，与现有的液滴致动器相比，本发明的结构和方法尤其提供了改进的效率，吞吐量，可扩展性和/或液滴均匀性。 此外，在一些实施例中，液滴致动器提供用于加载和/或卸载流体和/或液滴的改进方法的构造。 在其他实施例中，液滴致动器提供用于以基本上同时和/或基本顺序的方式装载多个流体储存器的流体加载构造。