公开(公告)号：US11898159B2

公开(公告)日：2024-02-13

申请号：US17125322

申请日：2020-12-17

申请人： University of Louisville Research Foundation, Inc.

发明人： Stuart K. Williams ,  Jeremy S. Touroo ,  James B. Hoying

IPC分类号： C12N5/00 ,  C12M3/00 ,  C12M1/26 ,  B33Y10/00 ,  B33Y80/00

CPC分类号： C12N5/0012 ,  C12M21/08 ,  C12M33/00 ,  C12N5/0062 ,  C12N5/0075 ,  B33Y10/00 ,  B33Y80/00 ,  C12N2513/00 ,  C12N2533/74

摘要： Methods of making a spheroid are provided whereby a suspension is first produced including one or more biologically-relevant materials dispersed within a biocompatible medium. A droplet of the suspension is then bioprinted into a salt solution by bringing the droplet into contact with a surface of the salt solution in a controlled manner to reproducibly yield a spheroid containing a desired size and a desired amount of biologically-relevant materials.

公开(公告)号：US11879837B2

公开(公告)日：2024-01-23

申请号：US17250124

申请日：2019-05-31

申请人： Photothermal Spectroscopy Corp.

发明人： Craig Prater ,  Derek Decker ,  Roshan Shetty

IPC分类号： G01N21/3563 ,  G06T7/00 ,  G02B21/06 ,  G02B21/36 ,  C12M1/00 ,  C12M1/26 ,  C12M1/34 ,  C12M1/36 ,  C12N1/12 ,  H04N23/74 ,  H04N23/741

CPC分类号： G01N21/3563 ,  C12M21/02 ,  C12M29/00 ,  C12M29/04 ,  C12M29/22 ,  C12M33/00 ,  C12M39/00 ,  C12M41/12 ,  C12M41/26 ,  C12M41/34 ,  C12M41/36 ,  C12M41/44 ,  C12M41/48 ,  C12N1/12 ,  G02B21/06 ,  G02B21/365 ,  G06T7/97 ,  H04N23/74 ,  H04N23/741 ,  G01N2201/061 ,  G01N2201/062 ,  G06T2207/10048 ,  G06T2207/10056 ,  G06T2207/10152

摘要： Apparatuses and methods for microscopic analysis of a sample by simultaneously characterizing infrared absorption characteristics of a plurality of spatially resolved locations are described herein. These apparatuses and methods improve sampling times while collecting microscopic data regarding composition of a sample across a wide field.

公开(公告)号：US11879119B2

公开(公告)日：2024-01-23

申请号：US17263712

申请日：2019-08-01

申请人： University of Florida Research Foundation, Inc.

发明人： Thomas Ettor Angelini ,  Tapomoy Bhattacharjee ,  Wallace Gregory Sawyer ,  Cameron Morley

IPC分类号： C12M1/12 ,  C12M1/26 ,  C12M1/00 ,  C12M1/32 ,  C12M1/20 ,  A01N1/02 ,  C12N5/00

CPC分类号： C12M25/04 ,  A01N1/0231 ,  C12M1/20 ,  C12M23/12 ,  C12M23/34 ,  C12M25/16 ,  C12M29/04 ,  C12M33/00 ,  C12N5/0018 ,  C12N5/0062

摘要： Disclosed herein is a bioreactor system that allows perfusive flow through a porous support medium enabling 3D growth of biological samples. In some embodiments, the system comprises a sample well filled with a three-dimensional (3D) cell growth medium. The system can further comprises a liquid medium reservoir fluidly connected to the sample well by a first filter material. The system can further comprises a medium collection chamber fluidly connected to the sample well by a second filter material. The system can further comprise an absorbant material that creates an osmotic pressure gradient to produce perfusive flow. In some embodiments, osmotic pressure draws fluid from the liquid medium reservoir, through the first filter material, into the sample well where it permeates the three-dimensional cell growth medium, through the second filter material, and finally into the medium collection chamber.

公开(公告)号：US20240010963A1

公开(公告)日：2024-01-11

申请号：US18255292

申请日：2021-12-01

申请人： CellSeed Inc.

发明人： Daishi Suga ,  Yuji Ueno ,  Yuzo Kasuya

IPC分类号： C12M1/26 ,  C12M1/00

CPC分类号： C12M33/00 ,  C12M23/22

摘要： Provided are a cell culture device for producing a cell sheet to be used in regenerative medicine and drug discovery research, and a kit for producing a cell sheet. Specifically, a cell detachment inhibition region having cell adhesive force stronger than that of a stimulus-responsive region regardless of the presence or absence of stimulation, and inhibiting cell detachment is directly provided over the entire circumference of a cell culture surface, and the stimulus-responsive region having adhesiveness to cells, which is varied due to an external stimulus, is arranged inside the cell detachment inhibition region, thereby forming a culture device for prevention of cell detachment due to mechanical stimulation such as medium exchange. Moreover, with a support for harvesting cell sheet to be used for the harvest of a cell sheet having given features, the cell sheet can be easily harvested.

公开(公告)号：US11866735B2

公开(公告)日：2024-01-09

申请号：US17688854

申请日：2022-03-07

申请人： Cellino Biotech, Inc.

发明人： Matthias Wagner ,  Suvi Aivio ,  Mariangela Amenduni ,  Catherine Pilsmaker ,  Arnaldo Pereira ,  Ananya Zutshi ,  Anthia Toure ,  Steven Nagle ,  Ozge Whiting ,  George Harb ,  Matthew Sullivan ,  Maya Berlin-Udi ,  Stefanie Morgan ,  Nick Seay ,  Sang Lee ,  Scott Luro

IPC分类号： G06T7/00 ,  G01N15/14 ,  C12N5/074 ,  C12N5/00 ,  C12M1/00 ,  C12M3/00 ,  C12M1/34 ,  C12M1/36 ,  C12M1/12 ,  C12M1/06 ,  C12M1/26 ,  G01N15/10 ,  B01L3/00 ,  B01L9/00

CPC分类号： C12N5/0696 ,  B01L3/502715 ,  B01L3/502761 ,  B01L9/52 ,  C12M23/14 ,  C12M23/22 ,  C12M23/26 ,  C12M23/28 ,  C12M23/42 ,  C12M23/44 ,  C12M23/48 ,  C12M25/06 ,  C12M27/02 ,  C12M29/02 ,  C12M33/00 ,  C12M33/12 ,  C12M41/12 ,  C12M41/26 ,  C12M41/44 ,  C12M41/46 ,  C12M41/48 ,  C12M47/04 ,  C12N5/0081 ,  G01N15/1056 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1475 ,  G06T7/0012 ,  G06T7/0016 ,  B01L2200/18 ,  B01L2300/0663 ,  B01L2300/0877 ,  C12M41/36 ,  G01N2015/1006 ,  G01N2015/1452 ,  G01N2015/1454 ,  G01N2015/1486 ,  G06T2207/10056 ,  G06T2207/10064 ,  G06T2207/20081 ,  G06T2207/30024 ,  G06T2207/30072

摘要： Disclosed herein are platforms, systems, and methods including a cell culture system that includes a cell culture container comprising a cell culture, the cell culture receiving input cells, a cell imaging subsystem configured to acquire images of the cell culture, a computing subsystem configured to perform a cell culture process on the cell culture according to the images acquired by the cell imaging subsystem, and a cell editing subsystem configured to edit the cell culture to produce output cell products according to the cell culture process.

公开(公告)号：US20230416663A1

公开(公告)日：2023-12-28

申请号：US18463595

申请日：2023-09-08

申请人： RAPID MICRO BIOSYSTEMS, INC.

发明人： Robert C. AVILES ,  Devin T. MICHAUD ,  Douglas J. BROWNE

IPC分类号： C12M3/00 ,  C12M1/00 ,  C12M1/22 ,  C12M1/12 ,  C12M1/26 ,  C12M1/34 ,  C12Q1/22

CPC分类号： C12M23/42 ,  C12M23/00 ,  C12M23/10 ,  C12M23/22 ,  C12M23/38 ,  C12M25/02 ,  C12M25/14 ,  C12M29/00 ,  C12M33/00 ,  C12M41/36 ,  C12Q1/22

摘要： The invention provides a device for growing cells—referred to as a cassette. The cell culturing device includes a housing that contains a lid having an optically clear window; a fluid distribution channel; a sample injection port fluidically connected to the fluid distribution channel; a base housing a porous media pad; and a media injection port fluidically connected to the media pad. The lid mates to the base to form a sterile seal; the fluid distribution channel is disposed over the media pad, which is viewable through the optical window; and sample fluid introduced into the fluid distribution channel is distributed evenly to the media pad, e.g., via a plurality of channels. The invention also provides kits that include cassettes of the invention and a tube set.

公开(公告)号：US11807895B2

公开(公告)日：2023-11-07

申请号：US15560977

申请日：2016-03-24

申请人： THE BROAD INSTITUTE, INC. ,  DANA-FARBER CANCER INSTITUTE, INC. ,  President and Fellows of Harvard College ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

发明人： Asaf Rotem ,  Kevin Struhl ,  Paul Blainey ,  Liyi Xu

IPC分类号： C12Q1/68 ,  G01N33/50 ,  G01N33/574 ,  C12M1/26

CPC分类号： C12Q1/68 ,  G01N33/5008 ,  G01N33/5011 ,  G01N33/574 ,  C12M33/00

摘要： A method for detecting oncogenic growth and viability, and/or degree of cellular transformation and/or identifying an agent that inhibits cellular transformation is disclosed. The method including: providing a cellular sample, such as a sample of cells obtained from a subject or a cell line; culturing the cellular sample in low attachment conditions; and detecting growth and7or cell viability of the sample, wherein increased growth relative and/or viability relative to a control or control level indicative of basal growth and/or viability indicates cellular transformation. In some embodiments, the method includes introducing a n expression vector into cells of the cellular sample, wherein the expression vector comprises a gene product expression sequence being tested for transformation ability. In some embodiments the cellular sample is contacted with a test agent and growth and/or cell viability of the sample is determined to determine if the agent inhibits transformation.

公开(公告)号：US20230348838A1

公开(公告)日：2023-11-02

申请号：US18348242

申请日：2023-07-06

申请人： Shanghai Longevity Co., Ltd.

发明人： Tian Xia ,  Ke Ma

IPC分类号： C12M1/36 ,  C12M1/00 ,  C12M1/26 ,  C12M3/00 ,  C12M1/12

CPC分类号： C12M41/48 ,  C12M23/22 ,  C12M33/00 ,  C12M23/48 ,  C12M25/02

摘要： A bioreactor includes a culture medium vessel housing culture medium. The culture medium vessel further includes a first side surface including a first transparent optical window; and a second side surface parallel to the first surface and including one or more sensor adapters to fix optical sensors. A cell retention vessel is disposed underneath and connected to the culture medium vessel, the cell retention vessel housing biological cells and having: a top surface that intersects a base of the culture medium vessel, and a second transparent optical window indented into the top surface at a first corner of the top surface. A semipermeable membrane is disposed at a bottom of the cell retention vessel, and a frame comprising a grid is disposed underneath the semipermeable membrane.

公开(公告)号：US20230332111A1

公开(公告)日：2023-10-19

申请号：US18339779

申请日：2023-06-22

申请人： Cellino Biotech, Inc.

发明人： Matthias WAGNER ,  Suvi AIVIO ,  Mariangela AMENDUNI ,  Catherine PILSMAKER ,  Arnaldo PEREIRA ,  Ananya ZUTSHI ,  Anthia TOURE ,  Steven NAGLE ,  Ozge WHITING ,  George HARB ,  Matthew SULLIVAN ,  Maya BERLIN-UDI ,  Stefanie MORGAN ,  Nick SEAY ,  Sang LEE ,  Scott LURO

IPC分类号： C12N5/074 ,  G06T7/00 ,  C12M1/00 ,  C12M1/36 ,  C12M1/12 ,  C12M1/34 ,  C12M3/00 ,  G01N15/10 ,  C12M1/26 ,  C12N5/00 ,  C12M1/06 ,  B01L3/00

CPC分类号： C12N5/0696 ,  G06T7/0012 ,  C12M23/14 ,  C12M41/48 ,  C12M47/04 ,  C12M25/06 ,  C12M41/44 ,  C12M23/44 ,  G01N15/1056 ,  C12M33/00 ,  C12M23/26 ,  C12M29/02 ,  C12M23/22 ,  C12M23/48 ,  C12M41/12 ,  C12M41/26 ,  C12M41/46 ,  C12M23/42 ,  C12M23/28 ,  C12N5/0081 ,  G06T7/0016 ,  C12M27/02 ,  C12M33/12 ,  B01L3/502715 ,  B01L2300/0663 ,  G06T2207/10064 ,  G06T2207/30024 ,  G06T2207/10056 ,  G01N2015/1006 ,  G06T2207/30072 ,  G06T2207/20081

摘要： Disclosed herein are platforms, systems, and methods including a cell culture system that includes a cell culture container comprising a cell culture, the cell culture receiving input cells, a cell imaging subsystem configured to acquire images of the cell culture, a computing subsystem configured to perform a cell culture process on the cell culture according to the images acquired by the cell imaging subsystem, and a cell editing subsystem configured to edit the cell culture to produce output cell products according to the cell culture process.

公开(公告)号：US20230332094A1

公开(公告)日：2023-10-19

申请号：US18212506

申请日：2023-06-21

申请人： GID BIO, Inc.

发明人： William W. Cimino ,  Thomas Michael Johannsen ,  David Joe Wesley ,  Dale William Tomrdle

IPC分类号： C12M1/00 ,  C12M3/00 ,  C12M1/06 ,  C12M3/04 ,  C12M1/26

CPC分类号： C12M45/09 ,  C12M23/28 ,  C12M23/44 ,  C12M27/02 ,  C12M27/10 ,  C12M33/00 ,  C12M33/14

摘要： A tissue processing system for dissociation of and release of cellular components from adipose tissue to prepare stromal vascular fraction includes a portable tissue processing unit for containing biological material including adipose tissue during enzymatic digestion processing and a digestion drive unit configured to receive the tissue processing unit for rotational processing of the tissue processing unit about an axis of rotation. The unit has an upright orientation and a reclined orientation in which the axis of rotation is at a reclined angle to horizontal relative to the upright orientation. The digestion drive unit is configured to receive and drive rotation of the tissue processing unit in the reclined orientation. Methods for processing biological material including adipose tissue include enzymatically digesting adipose tissue with rotation of a tissue processing unit around an axis of rotation in a reclined orientation.