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    BIOTINIDASE ASSAYS
    25.
    发明申请
    BIOTINIDASE ASSAYS 审中-公开
    生物测定测定

    公开(公告)号：US20150111237A1

    公开(公告)日：2015-04-23

    申请号：US14399051

    申请日：2013-05-07

    Applicant: ADVANCED LIQUID LOGIC, INC.

    Inventor： Carrie A. Graham ,  Allen E. Eckhardt

    IPC: C12Q1/34

    CPC classification number: C12Q1/34 ,  G01N33/542 ,  G01N33/582

    Abstract: The invention relates to methods for enzymatic detection of biotinidase activity. In certain aspects, the invention provides methods for bench-based enzymatic detection of biotinidase activity. In other aspects, the invention provides methods for bench-based enzymatic detection in droplets in oil. The droplet-based methods may be performed in an automated manner using digital microfluidics technology on a droplet actuator device. The enzymatic assays for biotinidase activity may be used for newborn testing for biotinidase deficiency, and may be combined with other droplet-based enzymatic assays in a panel of tests for newborn testing.

    Abstract translation: 本发明涉及酶检测生物素酶活性的方法。 在某些方面，本发明提供了用于基于酶的酶检测生物素酶活性的方法。 在其它方面，本发明提供了用于油中液滴的基于酶的酶检测方法。 基于液滴的方法可以使用液滴致动器装置上的数字微流体技术以自动方式进行。 用于生物素酶活性的酶测定可用于生物素酶缺陷的新生儿测试，并且可以在新生儿测试的一组测试中与其他基于液滴的酶测定组合。

    Techniques and Droplet Actuator Designs for Reducing Bubble Formation
    26.
    发明申请
    Techniques and Droplet Actuator Designs for Reducing Bubble Formation 有权
    技术和滴液致动器设计减少气泡形成

    公开(公告)号：US20150075986A1

    公开(公告)日：2015-03-19

    申请号：US14549113

    申请日：2014-11-20

    Applicant: ADVANCED LIQUID LOGIC, INC.

    Inventor： Delattre Cyril ,  Rival Arnaud ,  Srinivasan Vijay

    IPC: B01L3/02

    CPC classification number: B01L3/502784 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/5029 ,  B01L3/52 ,  B01L2200/06 ,  B01L2200/0605 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/088 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0442 ,  B01L2400/0448

    Abstract: During droplet operations in a droplet actuator, bubbles often form in the filler fluid in the droplet operations gap and interrupt droplet operations. The present invention provides methods and systems for performing droplet operations on a droplet in a droplet actuator comprising maintaining substantially consistent contact between the droplet and an electrical ground while conducting multiple droplet operations on the droplet in the droplet operations gap and/or reducing the accumulation of electrical charges in the droplet operations gap during multiple droplet operations. The methods and systems reduce or eliminate bubble formation in the filler fluid of the droplet operations gap, thereby permitting completion of multiple droplet operations without interruption by bubble formation in the filler fluid in the droplet operations gap.

    Abstract translation: 在液滴致动器中的液滴操作期间，液滴操作间隙中的气泡通常在填充液中形成并且中断液滴操作。 本发明提供了用于在液滴致动器中的液滴上进行液滴操作的方法和系统，包括在液滴操作间隙内对液滴进行多个液滴操作并且/或减少 在多个液滴操作期间液滴操作间隙中的电荷。 所述方法和系统减少或消除了液滴操作间隙的填充流体中的气泡形成，从而允许完成多个液滴操作，而不会由于液滴操作间隙中的填充流体中的气泡形成而中断。

    DROPLET OPERATIONS DEVICE
    27.
    发明申请
    DROPLET OPERATIONS DEVICE 审中-公开
    DROPLET操作装置

    公开(公告)号：US20150068903A1

    公开(公告)日：2015-03-12

    申请号：US14498418

    申请日：2014-09-26

    Applicant: Advanced Liquid Logic, Inc.

    Inventor： Vijay Srinivasan ,  Arjun Sudarsan ,  Vamsee K. Pamula ,  Michael G. Pollack ,  Ramakrishna Sista ,  Raymond Kozikowski ,  Nicole Bell

    IPC: G01N27/447

    CPC classification number: G01N27/44756 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502707 ,  B01L3/502792 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2400/0427 ,  B01L2400/0688 ,  B01L2400/086 ,  B29C59/02 ,  B41J2/1601 ,  B81B7/0061 ,  B81B2201/058 ,  B81B2201/13 ,  F04B17/00 ,  F04B19/006

    Abstract: The invention provides droplet actuators with droplet operations surfaces for manipulating droplets, e.g., by conducting droplet operations. The droplet operations surfaces are typically exposed to a droplet operations gap. One or more regions of a droplet operation surface may include patterned topographic features. The invention also provides a droplet actuator in which one or both gap-facing droplet operations surfaces is formed using a removable film. The removable film may, in various embodiments, also include other components ordinarily associated with the droplet actuator substrate, such as the dielectric layer and the electrodes. Further, the invention provides droplet actuator devices and methods for coupling and/or sealing substrates of a droplet actuator, such as techniques for self-aligning assembly of droplet actuator substrates. The invention provides droplet actuators and methods of disassembling the droplet actuator in order to provide access for cleaning and/or recycling of droplet actuator surfaces.

    Abstract translation: 本发明提供了液滴致动器，其具有用于操纵液滴的液滴操作表面，例如通过进行液滴操作。 液滴操作表面通常暴露于液滴操作间隙。 液滴操作表面的一个或多个区域可以包括图案化的地形特征。 本发明还提供一种液滴致动器，其中使用可去除的膜形成一个或两个面向间隙的液滴操作表面。 在各种实施例中，可除去膜可以包括通常与液滴致动器基底（例如电介质层和电极）相关联的其它部件。 此外，本发明提供了液滴致动器装置和用于联接和/或密封液滴致动器的基板的方法，例如用于液滴致动器基板的自对准组装的技术。 本发明提供了液滴致动器和拆卸液滴致动器的方法，以便为液滴致动器表面的清洁和/或回收提供通路。

    BEAD MANIPULATION TECHNIQUES
    29.
    发明申请
    BEAD MANIPULATION TECHNIQUES 审中-公开
    珠子操纵技术

    公开(公告)号：US20140193807A1

    公开(公告)日：2014-07-10

    申请号：US14163442

    申请日：2014-01-24

    Applicant: ADVANCED LIQUID LOGIC, INC.

    Inventor： VAMSEE K. PAMULA ,  ARJUN SUDARSAN ,  RAMAKRISHNA SISTA

    IPC: C12Q1/68 ,  G01N33/543

    CPC classification number: B03C7/026 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0887 ,  B01L2400/0424 ,  B01L2400/0427 ,  B03C1/01 ,  B03C1/288 ,  B03C5/00 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  G01N33/54326

    Abstract: The invention provides a method of redistributing magnetically responsive beads in a droplet. The method may include providing a droplet including magnetically responsive beads. The droplet may be provided within a region of a magnetic field having sufficient strength to attract the magnetically responsive beads to an edge of the droplet or towards an edge of the droplet, or otherwise regionalize or aggregate beads within the droplet. The method may also include conducting on a droplet operations surface one or more droplet operations using the droplet without removing the magnetically responsive beads from the region of the magnetic field. The droplet operations may in some cases be electrode-mediated. The droplet operations may redistribute and/or circulate the magnetically responsive beads within the droplet. In some cases, the droplet may include a sample droplet may include a target analyte. The redistributing of the magnetically responsive beads may cause target analyte to bind to the magnetically responsive beads. In some cases, the droplet may include unbound substances in a wash buffer. The redistributing of the magnetically responsive beads causes unbound substances to be freed from interstices of an aggregated set or subset of the magnetically responsive beads.

    Abstract translation: 本发明提供了一种在液滴中重新分布磁响应珠的方法。 该方法可以包括提供包括磁响应珠的液滴。 液滴可以设置在具有足够强度的磁场的区域内，以将磁响应珠吸引到液滴的边缘或朝向液滴的边缘，或以其他方式区分或聚集液滴内的珠粒。 该方法还可以包括在液滴操作表面上使用液滴进行一个或多个液滴操作，而不从磁场的区域移除磁响应珠。 在某些情况下，液滴操作可能是电极介导的。 液滴操作可以在液滴内重新分布和/或循环磁响应珠。 在一些情况下，液滴可以包括样品液滴可以包括靶分析物。 磁响应珠的重新分布可能导致目标分析物结合磁响应珠。 在一些情况下，液滴可以包括洗涤缓冲液中的未结合物质。 磁响应珠子的重新分布导致未结合物质从磁性响应珠粒的聚集或子集的空隙中释放出来。

    SYSTEM AND METHOD OF DISPENSING LIQUIDS IN A MICROFLUIDIC DEVICE
    30.
    发明申请
    SYSTEM AND METHOD OF DISPENSING LIQUIDS IN A MICROFLUIDIC DEVICE 审中-公开
    微流控装置中液体分配系统及方法

    公开(公告)号：US20140161686A1

    公开(公告)日：2014-06-12

    申请号：US14102367

    申请日：2013-12-10

    Applicant: Advanced Liquid Logic, Inc.

    Inventor： Donovan E. BORT ,  Vijay SRINIVASAN ,  Patrick STERLINA ,  Uichong B. YI ,  Justin CAPPELLETTI

    IPC: B01L3/00

    CPC classification number: B01L3/527 ,  B01L3/502715 ,  B01L3/502784 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0673 ,  B01L2200/16 ,  B01L2300/045 ,  B01L2300/049 ,  B01L2300/0672 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/1827 ,  B01L2400/0427 ,  B01L2400/0644 ,  B01L2400/065 ,  B01L2400/0683

    Abstract: Microfluidic system including a droplet actuator having an interior cavity and a series of electrodes arranged along the interior cavity for forming a droplet-operation path therethrough. The droplet actuator has a module-engaging side including an opening that is in flow communication with the interior cavity. The microfluidic system also includes a reservoir module configured to be coupled to the droplet actuator. The reservoir module includes a plurality of liquid compartments having respective outlets and at least one seal positioned along the outlets to retain liquid within the liquid compartments. The reservoir module is movable along the module-engaging side of the droplet actuator to position the outlets relative to the opening. The microfluidic system also includes a piercer having a tip configured to penetrate the seal thereby permitting the liquid within the corresponding liquid compartment to flow into the opening.

    Abstract translation: 微流体系统包括具有内腔的液滴致动器和沿内腔布置的一系列电极，用于形成通过其的液滴操作路径。 液滴致动器具有包括与内腔流动连通的开口的模块接合侧。 微流体系统还包括被配置为耦合到液滴致动器的贮存器模块。 储存器模块包括多个具有相应出口的液体隔室和沿出口定位的至少一个密封件，以将液体保持在液体隔间内。 储存器模块可沿液滴致动器的模块接合侧移动，以使出口相对于开口定位。 微流体系统还包括具有尖端的穿孔器，该尖端被配置为穿透密封，从而允许相应的液体隔室内的液体流入开口。

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