Directed Evolution and Selection Using in Vitro Compartmentalization
    22.
    发明申请
    Directed Evolution and Selection Using in Vitro Compartmentalization 审中-公开
    导向进化和选择使用体外分区

    公开(公告)号:US20080004436A1

    公开(公告)日:2008-01-03

    申请号:US11718929

    申请日:2005-11-15

    CPC classification number: C12N15/1075

    Abstract: The present invention is related to the field of compartmentalized libraries of genetic elements and the selection of biologically active molecules and the genes encoding same from said libraries. The selection assay of the invention utilizes water-in-oil emulsions and is particularly advantageous in applications in the field of directed-evolution, as exemplified herein for selection of protein inhibitors of DNA nucleases.

    Abstract translation: 本发明涉及遗传元件的区室化文库领域以及生物活性分子的选择以及从所述文库编码相同的基因。 本发明的选择测定法使用油包水乳剂,并且在定向进化领域中的应用中是特别有利的,如本文所例示的用于选择DNA核酸酶的蛋白质抑制剂。

    Sunscreen composition containing sol-gel microcapsules
    23.
    发明授权
    Sunscreen composition containing sol-gel microcapsules 有权
    含有溶胶 - 凝胶微胶囊的防晒组合物

    公开(公告)号:US06238650B1

    公开(公告)日:2001-05-29

    申请号:US09318828

    申请日:1999-05-26

    Abstract: The present invention generally relates to safe and stable sunscreen compositions comprising of at least one sunscreen active ingredient in the form of an inert sol-gel microcapsules encapsulating ultraviolet absorbing compounds in any acceptable cosmetic vehicle. The composition according to the present invention can comprise several ultraviolet absorbers that may be encapsulated in the same sol-gel microcapsule or in different capsules. The encapsulation of the ultraviolet absorbers reduces or even prevents the contact between the sunscreen compounds and the human tissue, thus reducing various adverse effects that are associated with the use of sunscreens, such as photoallergy and phototoxicity, and makes the composition safer for use. The encapsulation also reduces or even prevents cross reactivity between the sunscreen compounds and the packaging material and between the sunscreen compounds and any other component present in the composition, thus enhance the compositions stability. The hydophobicity/hydrophilicity character of the sol-gel microcapsules can be controlled by selecting suitable sol-gel precursors and suitable reaction conditions and can be chosen to be compatible with the cosmetic vehicle to be used in the sunscreen composition, thus, the present invention facilitates an easy incorporation of the composite sol-gel encapsulated sunscreen in all types of cosmetic vehicles including oil free compositions, with no necessary steps of heating or high shear forces. The sunscreen compositions of the present invention can comprise any acceptable UVA and/or UVB absorbing compounds at any desired ratio to obtain a desired accumulative ultraviolet screening spectrum.

    Abstract translation: 本发明一般涉及安全和稳定的防晒组合物,其包含至少一种防霉活性成分,其形式为在任何可接受的化妆品载体中包封紫外线吸收化合物的惰性溶胶 - 凝胶微胶囊形式。 根据本发明的组合物可以包含几种可以包封在相同溶胶 - 凝胶微胶囊或不同胶囊中的紫外线吸收剂。 紫外线吸收剂的封装减少或甚至防止防晒化合物与人体组织之间的接触,从而减少与防晒剂的使用相关的各种不利影响,例如光过敏和光毒性,并使组合物更安全地使用。 封装还降低或甚至防止防晒化合物和包装材料之间以及防晒化合物和组合物中存在的任何其它组分之间的交叉反应性,从而增强组合物的稳定性。 可以通过选择合适的溶胶 - 凝胶前体和合适的反应条件来控制溶胶 - 凝胶微胶囊的疏水性/亲水性,并且可以选择与化妆品相容以用于防晒组合物,因此本发明有利于 将复合溶胶 - 凝胶包胶的防晒剂容易地并入包括无油组合物的所有类型的化妆品载体中,没有必要的加热步骤或高剪切力。 本发明的防晒组合物可以以任何所需的比例包含任何可接受的UVA和/或UVB吸收化合物,以获得所需的累积紫外线屏蔽谱。

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