公开(公告)号：US20200299351A1

公开(公告)日：2020-09-24

申请号：US16743849

申请日：2020-01-15

Applicant: Berkeley Lights, Inc.

Inventor： Peter J. Beemiller ,  Alexander J. Mastroianni ,  Shao Ning Pei ,  Randall D. Lowe, JR. ,  Annamaria Mocciaro ,  Kevin D. Loutherback ,  Yelena Bronevetsky ,  Guido K. Stadler ,  Andrew W. McFarland ,  Kevin T. Chapman ,  Duane Smith ,  Natalie C. Marks ,  Amanda L. Goodsell

IPC: C07K14/725 ,  C07K16/28 ,  G01N33/50 ,  C07K14/74

Abstract: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to activate T Lymphocytes.

公开(公告)号：US20200078788A1

公开(公告)日：2020-03-12

申请号：US16509166

申请日：2019-07-11

Applicant: Berkeley Lights, Inc.

Inventor： Kevin T. Chapman ,  Daniele Malleo ,  J. Tanner Nevill ,  Steven W. Short ,  Mark P. White ,  M. Jimena Loureiro

IPC: B01L3/00 ,  B03C5/02 ,  B03C5/00 ,  G01N15/14 ,  G01N33/50 ,  G01N33/543 ,  G01N33/68

Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.

公开(公告)号：US20180135011A1

公开(公告)日：2018-05-17

申请号：US15802100

申请日：2017-11-02

Applicant: Berkeley Lights, Inc.

Inventor： Yelena Bronevetsky ,  Xiaohua Wang ,  Peter J. Beemiller ,  Kristin G. Beaumont ,  Randall D. Lowe, JR. ,  Alexander J. Mastroianni ,  Kevin T. Chapman

IPC: C12N5/0783 ,  A61P35/00 ,  C12M3/06 ,  A61K39/00 ,  C12M1/00

CPC classification number: C12N5/0636 ,  A61K35/17 ,  A61K39/0011 ,  A61K2035/124 ,  A61K2039/5158 ,  A61P35/00 ,  C12M23/16 ,  C12M29/10 ,  C12N2501/2302 ,  C12N2501/51 ,  C12N2501/515 ,  C12N2502/1121 ,  C12N2533/50

Abstract: Methods of expanding T lymphocytes in a microfluidic device are provided. The methods can include introducing one or more T lymphocytes into a microfluidic device; contacting the one or more T lymphocytes with an activating agent; and perfusing culture medium through the microfluidic device for a period of time sufficient to allow the one or more T lymphocytes to undergo at least one round of mitotic cell division. The expansion can be non-specific or antigen-specific. T lymphocytes produced according to the disclosed methods are also provided, along with methods of treating cancer in a subject. The methods of treating cancer can include isolating T lymphocytes from a tissue sample obtained from the subject; expanding the isolated T lymphocytes in a microfluidic device; exporting the expanded T lymphocytes from the microfluidic device; and reintroducing the expanded T lymphocytes into the subject.

公开(公告)号：US09857333B2

公开(公告)日：2018-01-02

申请号：US14060117

申请日：2013-10-22

Applicant: Berkeley Lights, Inc.

Inventor： Kevin T. Chapman ,  Igor Y. Khandros ,  Gaetan L. Mathieu ,  J. Tanner Nevill ,  Ming C. Wu

IPC: B01L3/00 ,  G01N27/447 ,  G01N27/26 ,  B03C5/00 ,  B03C5/02

CPC classification number: G01N27/44791 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0877 ,  B01L2400/0424 ,  B01L2400/0454 ,  B01L2400/086 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26

Abstract: Individual biological micro-objects can be deterministically selected and moved into holding pens in a micro-fluidic device. A flow of a first liquid medium can be provided to the pens. Physical pens can be structured to impede a direct flow of the first medium into a second medium in the pens while allowing diffusive mixing of the first medium and the second medium. Virtual pens can allow a common flow of medium to multiple ones of the pens.

公开(公告)号：US20170021366A1

公开(公告)日：2017-01-26

申请号：US15105849

申请日：2014-12-18

Applicant: Berkeley Lights, Inc.

Inventor： Kevin T. Chapman ,  Eric D. Hobbs ,  Steven W. Short ,  Mark P. White ,  Daniele Malleo

IPC: B03C5/00 ,  B03C11/00 ,  B01L3/00 ,  B03C5/02

CPC classification number: B03C5/005 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2200/14 ,  B01L2400/0424 ,  B03C5/026 ,  B03C11/00 ,  B03C2201/26

Abstract: Individual biological cells can be selected in a micro-fluidic device and moved into isolation pens in the device. The cells can then be lysed in the pens, releasing nucleic acid material, which can be captured by one or more capture objects in the pens. The capture objects with the captured nucleic acid material can then be removed from the pens. The capture objects can include unique identifiers, allowing each capture object to be correlated to the individual cell from which the nucleic acid material captured by the object originated.

Abstract translation: 可以在微流体装置中选择各种生物细胞，并将其移动到装置中的隔离笔中。 然后，细胞可以在笔中裂解，从而释放核酸物质，其可被笔中的一个或多个捕获物捕获。 然后可以从笔中取出捕获的核酸材料的捕获物体。 捕获对象可以包括唯一的标识符，允许每个捕获对象与由对象捕获的核酸材料源自的单个细胞相关联。

公开(公告)号：US12102083B2

公开(公告)日：2024-10-01

申请号：US17228058

申请日：2021-04-12

Applicant: BERKELEY LIGHTS, INC.

Inventor： Mark P. White ,  Kevin T. Chapman ,  Andrew W. McFarland ,  Eric D. Hobbs ,  Randall D. Lowe, Jr.

IPC: A01N1/02 ,  B01L3/00 ,  C12M1/00

CPC classification number: A01N1/0284 ,  A01N1/0221 ,  A01N1/0263 ,  B01L3/502715 ,  B01L3/502761 ,  C12M47/04 ,  B01L2200/0668 ,  B01L2300/021 ,  B01L2300/024 ,  B01L2300/0864 ,  B01L2300/16 ,  B01L2300/163 ,  B01L2300/18 ,  B01L2300/1894

Abstract: A method of processing and storing biological cells includes introducing a flowable medium into a microfluidic device, the flowable medium including biological cells; sequestering one or more biological cells from the flowable medium in one or more isolation regions of the microfluidic device; and freezing the microfluidic device including the one or more biological cells sequestered therein.

公开(公告)号：US11365381B2

公开(公告)日：2022-06-21

申请号：US16920365

申请日：2020-07-02

Applicant: Berkeley Lights, Inc.

Inventor： Randall D. Lowe, Jr. ,  Kristin G. Beaumont ,  Aathavan Karunakaran ,  Natalie C. Marks ,  Jason M. McEwen ,  Mark P. White ,  J. Tanner Nevill ,  Gang F. Wang ,  Andrew W. McFarland ,  Daniele Malleo ,  Keith J. Breinlinger ,  Xiao Guan ,  Kevin T. Chapman

IPC: C12M3/00 ,  B01L3/00 ,  C12M3/06 ,  C12M1/00

Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.

公开(公告)号：US20210270817A1

公开(公告)日：2021-09-02

申请号：US17198833

申请日：2021-03-11

Applicant: Berkeley Lights, Inc.

Inventor： Paul M. Lebel ,  Troy A. Lionberger ,  Kevin T. Chapman

IPC: G01N33/53 ,  B01L3/00

Abstract: Disclosed herein is a method for assaying binding affinity between a first molecule and a second molecule in a micro-fluidic device.

公开(公告)号：US20200284756A1

公开(公告)日：2020-09-10

申请号：US16869399

申请日：2020-05-07

Applicant: Berkeley Lights, Inc.

Inventor： Kevin T. Chapman ,  Igor Y. Khandros ,  Gaetan L. Mathieu ,  J. Tanner Nevill ,  Ming C. Wu

IPC: G01N27/447 ,  B01L3/00 ,  B03C5/00 ,  B03C5/02

Abstract: Individual biological micro-objects can be deterministically selected and moved into holding pens in a micro-fluidic device. A flow of a first liquid medium can be provided to the pens. Physical pens can be structured to impede a direct flow of the first medium into a second medium in the pens while allowing diffusive mixing of the first medium and the second medium. Virtual pens can allow a common flow of medium to multiple ones of the pens.

公开(公告)号：US10690628B2

公开(公告)日：2020-06-23

申请号：US15842124

申请日：2017-12-14

Applicant: Berkeley Lights, Inc.

Inventor： Kevin T. Chapman ,  Igor Y. Khandros ,  Gaetan L. Mathieu ,  J. Tanner Nevill ,  Ming C. Wu

IPC: G01N27/447 ,  B01L3/00 ,  B03C5/00 ,  B03C5/02 ,  G01N27/416 ,  G01N27/26

Abstract: Individual biological micro-objects can be deterministically selected and moved into holding pens in a micro-fluidic device. A flow of a first liquid medium can be provided to the pens. Physical pens can be structured to impede a direct flow of the first medium into a second medium in the pens while allowing diffusive mixing of the first medium and the second medium. Virtual pens can allow a common flow of medium to multiple ones of the pens.