公开(公告)号：US20250155430A1

公开(公告)日：2025-05-15

申请号：US18951459

申请日：2024-11-18

Applicant: ADVANCED LIQUID LOGIC, INC. ,  DUKE UNIVERSITY

Inventor： Ramakrishna Sista ,  Vamsee K. Pamula ,  Michael G. Pollack ,  Vijay Srinivasan ,  Allen E. Eckhardt ,  Richard B. Fair

IPC: G01N33/543 ,  B01L3/00 ,  C12M1/12 ,  G01N27/447 ,  G01N35/00 ,  G01N35/10

Abstract: A method comprising effecting a change in a shape of a droplet, wherein the droplet is disposed over a substrate in sensing proximity to a sensor and the droplet has a starting surface area exposed to the sensor; and producing an expanded surface area of the droplet in the sensing proximity exposed to the sensor, wherein the expanded surface area exposed to the sensor is greater than the starting surface area exposed to the sensor.

公开(公告)号：US09638662B2

公开(公告)日：2017-05-02

申请号：US14817834

申请日：2015-08-04

Applicant: Duke University

Inventor： Michael G. Pollack ,  Vamsee K. Pamula ,  Richard B. Fair

IPC: B01L3/00 ,  G01N27/447 ,  B01F13/00 ,  B01L7/00 ,  B81B3/00 ,  B81C1/00 ,  C12Q1/68 ,  G05D7/06 ,  H01L21/67

CPC classification number: G01N27/44769 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502707 ,  B01L3/502792 ,  B01L7/52 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/0645 ,  B01L2300/0663 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/12 ,  B01L2300/1827 ,  B01L2400/0415 ,  B01L2400/0424 ,  B81B3/0021 ,  B81B2201/058 ,  B81C1/00119 ,  C12Q1/6846 ,  G01N27/447 ,  G01N27/44791 ,  G05D7/0694 ,  H01L21/6715 ,  Y10T436/2575

Abstract: Apparatuses and methods for manipulating droplets are disclosed. In one embodiment, an apparatus for manipulating droplets is provided, the apparatus including a substrate, multiple arrays of electrodes disposed on the substrate, wherein corresponding electrodes in each array are connected to a common electrical signal, and a dielectric layer disposed on the substrate first side surface and patterned to cover the electrodes.

公开(公告)号：US09452433B2

公开(公告)日：2016-09-27

申请号：US14290057

申请日：2014-05-29

Applicant: Advanced Liquid Logic, Inc. ,  Duke University

Inventor： Alexander Shenderov ,  Michael G. Pollack

IPC: B01L7/00 ,  B01L3/00

CPC classification number: B01L7/525 ,  B01L3/502792 ,  B01L2200/0673 ,  B01L2300/0654 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/1816 ,  B01L2300/1827 ,  B01L2300/1872 ,  B01L2400/0427

Abstract: Methods and devices for conducting chemical or biochemical reactions that require multiple reaction temperatures are described. The methods involve moving one or more reaction droplets or reaction volumes through various reaction zones having different temperatures on a microfluidics apparatus. The devices comprise a microfluidics apparatus comprising appropriate actuators capable of moving reaction droplets or reaction volumes through the various reaction zones.

公开(公告)号：US20160016172A1

公开(公告)日：2016-01-21

申请号：US14837133

申请日：2015-08-27

Applicant: ADVANCED LIQUID LOGIC, INC. ,  DUKE UNIVERSITY

Inventor： Michael G. Pollack ,  Philip Paik ,  Vamsee K. Pamula

IPC: B01L7/00 ,  G01N27/447

CPC classification number: C12Q1/68 ,  B01L7/52 ,  B01L2300/0645 ,  B01L2300/1822 ,  B01L2300/1894 ,  B01L2400/0424 ,  B01L2400/0427 ,  C12Q1/6869 ,  G01N27/44791 ,  Y10T436/25 ,  C12Q2565/629 ,  C12Q2565/301 ,  C12Q2563/149

Abstract: Aspects of embodiments of the invention relate to a simulator including a visual display capable of outputting to a user a display one or more effects of a command series selected and a system including a droplet microactuator electronically coupled to and controlled by a processor capable of executing instructions, the droplet microactuator including a substrate comprising electrodes for conducting droplet operations. Further aspects of embodiments of the invention relate to a droplet operations troubleshooting apparatus. Other aspects of embodiments of the invention relate to a computer implemented method of displaying simulated microactuator droplets.

Abstract translation: 本发明的实施例的方面涉及一种模拟器，其包括能够向用户输出显示所选择的命令序列的一个或多个效果的视觉显示器，以及包括电子耦合到能够执行指令的处理器并由其控制的液滴微致动器的系统 液滴微致动器包括包括用于进行液滴操作的电极的基板。 本发明的实施例的其它方面涉及一种液滴操作故障排除装置。 本发明的实施例的其他方面涉及一种显示模拟微致动器液滴的计算机实现的方法。

公开(公告)号：US09139865B2

公开(公告)日：2015-09-22

申请号：US13680979

申请日：2012-11-19

Applicant: Advanced Liquid Logic, Inc. ,  Duke University

Inventor： Michael G. Pollack ,  Philip Paik ,  Vamsee K. Pamula

IPC: G05D5/06 ,  C12Q1/68 ,  G01N27/447

CPC classification number: C12Q1/68 ,  B01L7/52 ,  B01L2300/0645 ,  B01L2300/1822 ,  B01L2300/1894 ,  B01L2400/0424 ,  B01L2400/0427 ,  C12Q1/6869 ,  G01N27/44791 ,  Y10T436/25 ,  C12Q2565/629 ,  C12Q2565/301 ,  C12Q2563/149

Abstract: Aspects of embodiments of the invention relate to a simulator including a visual display capable of outputting to a user a display one or more effects of a command series selected and a system including a droplet microactuator electronically coupled to and controlled by a processor capable of executing instructions, the droplet microactuator including a substrate comprising electrodes for conducting droplet operations. Further aspects of embodiments of the invention relate to a droplet operations troubleshooting apparatus. Other aspects of embodiments of the invention relate to a computer implemented method of displaying simulated microactuator droplets.

Abstract translation: 本发明的实施例的方面涉及一种模拟器，其包括能够向用户输出显示所选择的命令序列的一个或多个效果的视觉显示器，以及包括电子耦合到能够执行指令的处理器并由控制器执行指令的液滴微致动器的系统 液滴微致动器包括包括用于进行液滴操作的电极的基板。 本发明的实施例的其它方面涉及一种液滴操作故障排除装置。 本发明的实施例的其他方面涉及一种显示模拟微致动器液滴的计算机实现的方法。

公开(公告)号：US20150148238A1

公开(公告)日：2015-05-28

申请号：US14609744

申请日：2015-01-30

Applicant: Advanced Liquid Logic, Inc. ,  Duke University

Inventor： Vamsee Pamula ,  Vijay Srinivasan ,  Michael G. Pollack ,  Richard B. Fair

IPC: B01L3/00 ,  B01L7/00 ,  G01N27/447 ,  C12Q1/68

CPC classification number: G01N27/26 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/0268 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2200/0684 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0442 ,  B01L2400/0448 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N27/3271 ,  G01N27/416 ,  G01N27/447 ,  G01N35/00029 ,  G01N2035/00158 ,  G01N2035/00237

Abstract: The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of splitting a droplet is provided, the method including providing a droplet microactuator including a droplet including one or more beads and immobilizing at least one of the one or more beads. The method further includes conducting one or more droplet operations to divide the droplet to yield a set of droplets including a droplet including the one or more immobilized beads and a droplet substantially lacking the one or more immobilized beads.

Abstract translation: 本发明涉及液滴表面改性和洗涤。 根据一个实施例，提供了一种分离液滴的方法，所述方法包括提供包括包含一个或多个珠并且固定所述一个或多个珠中的至少一个珠的液滴的液滴微致动器。 该方法还包括进行一个或多个液滴操作以分开液滴以产生一组液滴，包括包含一个或多个固定化珠粒的液滴和基本上不含一个或多个固定化珠粒的液滴。

公开(公告)号：US20150072900A1

公开(公告)日：2015-03-12

申请号：US14498378

申请日：2014-09-26

Applicant: ADVANCED LIQUID LOGIC, INC. ,  DUKE UNIVERSITY

Inventor： Vijay Srinivasan ,  Vamsee K. Pamula ,  Michael G. Pollack

IPC: B01L3/00 ,  B01J19/00

CPC classification number: B01L3/502784 ,  B01J19/0046 ,  B01J2219/00466 ,  B01J2219/00495 ,  B01J2219/00587 ,  B01J2219/00725 ,  B01L3/502761 ,  B01L2200/0642 ,  B01L2200/0647 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2300/0893 ,  B01L2300/18 ,  B01L2400/0427 ,  B01L2400/043 ,  C07K1/1136 ,  C30B7/00 ,  C30B29/58 ,  Y10T436/2575

Abstract: Sample processing droplet actuators, systems and methods are provided. According to one embodiment, a stamping device including a droplet microactuator is provided and includes: (a) a first plate including a path or network of control electrodes for transporting droplets on a surface thereof; (b) a second plate mounted in a substantially parallel orientation with respect to the first plate providing an interior volume between the plates, the second plate including one or more stamping ports for transporting some portion or all of a droplet from the interior volume to an exterior location; (c) a port for introducing fluid into the interior volume between the plates; and (d) a path or network of reference electrodes corresponding to the path or network of control electrodes. Associated systems and methods including the stamping device are also provided.

Abstract translation: 提供样品处理液滴致动器，系统和方法。 根据一个实施例，提供了一种包括液滴微致动器的冲压装置，其包括：（a）包括用于在其表面上输送液滴的控制电极的路径或网络的第一板; （b）相对于第一板以基本上平行的方向安装的第二板，提供板之间的内部容积，第二板包括一个或多个冲压端口，用于将一些部分或全部液滴从内部容积输送到 外部位置; （c）用于将流体引入板之间的内部容积的端口; 和（d）对应于控制电极的路径或网络的参考电极的路径或网络。 还提供了包括冲压装置在内的相关系统和方法。

公开(公告)号：US20140339082A1

公开(公告)日：2014-11-20

申请号：US14446733

申请日：2014-07-30

Applicant: DUKE UNIVERSITY

Inventor： Michael G. Pollack ,  Vamsee K. Pamula ,  Richard B. Fair

IPC: B01L3/00

CPC classification number: B01L3/50273 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0084 ,  B01L3/00 ,  B01L3/5027 ,  B01L3/502784 ,  B01L3/502792 ,  B01L2200/06 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2300/165 ,  B01L2400/0415 ,  B01L2400/0427 ,  B41J2002/14395 ,  C25B9/00 ,  G01N27/44791 ,  G02B26/005

Abstract: Methods are provided for manipulating droplets. The methods include providing the droplet on a surface comprising an array of electrodes and a substantially co-planer array of reference elements, wherein the droplet is disposed on a first one of the electrodes, and the droplet at least partially overlaps a second one of the electrodes and an intervening one of the reference elements disposed between the first and second electrodes. The methods further include activating the first and second electrodes to spread at least a portion of the droplet across the second electrode and deactivating the first electrode to move the droplet from the first electrode to the second electrode.

Abstract translation: 提供了用于操纵液滴的方法。 所述方法包括在包括电极阵列和基本上共平面的参考元件阵列的表面上提供液滴，其中液滴设置在电极的第一个上，并且液滴至少部分地与第二个 电极和设置在第一和第二电极之间的参考元件中的插入件之一。 所述方法还包括激活第一和第二电极以将液滴的至少一部分扩散穿过第二电极，并且去激活第一电极以使液滴从第一电极移动到第二电极。