公开(公告)号：US6090251A

公开(公告)日：2000-07-18

申请号：US870944

申请日：1997-06-06

申请人： Steven A. Sundberg ,  J. Wallace Parce ,  Calvin Y. H. Chow

发明人： Steven A. Sundberg ,  J. Wallace Parce ,  Calvin Y. H. Chow

IPC分类号： B01J19/00 ,  B01L3/00 ,  B01L3/02 ,  G01N27/447 ,  G01N35/00 ,  G01N35/10 ,  G01N27/26

CPC分类号： G01N27/44791 ,  B01J19/0093 ,  B01L3/0244 ,  B01L3/0262 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  G01N27/44743 ,  B01J2219/00783 ,  B01J2219/00826 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00853 ,  B01J2219/00891 ,  B01J2219/00909 ,  B01L2200/027 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0487 ,  B01L2400/0688 ,  B01L3/502723 ,  B01L3/502753 ,  G01N2035/00237 ,  G01N2035/1037 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/2575

摘要： Fluid introduction is facilitated through the use of a port which extends entirely through a microfluidic substrate. Capillary forces can be used to retain the fluid within the port, and a series of samples or other fluids may be introduced through a single port by sequentially blowing the fluid out through the substrate and replacing the removed fluid with an alternate fluid, or by displacing the fluid in part with additional fluid. In another aspect, microfluidic substrates have channels which varying in cross-sectional dimension so that capillary action spreads a fluid only within a limited portion of the channel network. In yet another aspect, the introduction ports may include a multiplicity of very small channels leading from the port to a fluid channel, so as to filter out particles or other contaminants which might otherwise block the channel at the junction between the channel and the introduction port.

公开(公告)号：US5624711A

公开(公告)日：1997-04-29

申请号：US431196

申请日：1995-04-27

申请人： Steven A. Sundberg ,  David Fujimoto

发明人： Steven A. Sundberg ,  David Fujimoto

IPC分类号： B01J19/00 ,  B05D1/18 ,  C03C17/30 ,  C03C17/34 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N33/543 ,  B05D1/36

CPC分类号： B82Y30/00 ,  B01J19/0046 ,  B05D1/185 ,  B82Y40/00 ,  C03C17/30 ,  C03C17/3405 ,  C40B80/00 ,  G01N33/54353 ,  B01J2219/00364 ,  B01J2219/00378 ,  B01J2219/0043 ,  B01J2219/005 ,  B01J2219/00504 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/0059 ,  B01J2219/00592 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00619 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01J2219/00725 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14

摘要： Methods and derivatized supports which are useful in solid-phase synthesis of peptides, oligonucleotides or other small organic molecules as well as arrays of ligands. The methods provide means to control the functional site density on a solid support. Some of the derivatized supports are polymer-coated or glycan-coated. Other methods for regenerating the surface of a used ligand array are also provided.

摘要翻译： 可用于肽，寡核苷酸或其他小有机分子的固相合成以及配体阵列的方法和衍生的载体。 该方法提供了控制固体支持物上的功能位点密度的方法。 一些衍生的载体是聚合物涂覆的或聚糖包被的。 还提供了用于再生所用配体阵列的表面的其它方法。

公开(公告)号：US20120031758A1

公开(公告)日：2012-02-09

申请号：US13273934

申请日：2011-10-14

申请人： Steven A. Sundberg ,  Xing Su ,  Grace Credo

发明人： Steven A. Sundberg ,  Xing Su ,  Grace Credo

IPC分类号： C25B7/00 ,  G01N27/447

CPC分类号： C12N15/1006

摘要： Embodiments of the invention provide devices and methods for extracting nucleic acid molecules from solution using electric fields. The structures and methods of embodiments of the invention are suited to incorporation into micro and nano fluidic devices, such as lab-on-a-chip devices and micro total analysis systems.

摘要翻译： 本发明的实施方案提供了使用电场从溶液中提取核酸分子的装置和方法。 本发明的实施例的结构和方法适合于并入微纳米流体装置，例如片上实验室装置和微总体分析系统。

公开(公告)号：US07364916B2

公开(公告)日：2008-04-29

申请号：US11617809

申请日：2006-12-29

申请人： Steven A. Sundberg ,  J. Wallace Parce ,  Calvin Y. H. Chow

发明人： Steven A. Sundberg ,  J. Wallace Parce ,  Calvin Y. H. Chow

IPC分类号： G01N1/10 ,  B01L3/02

CPC分类号： G01N27/44791 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00826 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00853 ,  B01J2219/00891 ,  B01J2219/00909 ,  B01L3/0244 ,  B01L3/0262 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/502753 ,  B01L2200/027 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0487 ,  B01L2400/0688 ,  G01N27/44743 ,  G01N2035/00237 ,  G01N2035/1037 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/2575

摘要： Fluid introduction is facilitated through the use of a port which extends entirely through a microfluidic substrate. Capillary forces can be used to retain the fluid within the port, and a series of samples or other fluids may be introduced through a single port by sequentially blowing the fluid out through the substrate and replacing the removed fluid with an alternate fluid, or by displacing the fluid in part with additional fluid. In another aspect, microfluidic substrates have channels which varying in cross-sectional dimension so that capillary action spreads a fluid only within a limited portion of the channel network. In yet another aspect, the introduction ports may include a multiplicity of very small channels leading from the port to a fluid channel, so as to filter out particles or other contaminants which might otherwise block the channel at the junction between the channel and the introduction port.

公开(公告)号：US07364705B2

公开(公告)日：2008-04-29

申请号：US11617795

申请日：2006-12-29

申请人： Steven A. Sundberg ,  J. Wallace Parce ,  Calvin Y. H. Chow

发明人： Steven A. Sundberg ,  J. Wallace Parce ,  Calvin Y. H. Chow

IPC分类号： G01N1/10 ,  B01L3/02

CPC分类号： G01N27/44791 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00826 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00853 ,  B01J2219/00891 ,  B01J2219/00909 ,  B01L3/0244 ,  B01L3/0262 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/502753 ,  B01L2200/027 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0487 ,  B01L2400/0688 ,  G01N27/44743 ,  G01N2035/00237 ,  G01N2035/1037 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/2575

摘要： Fluid introduction is facilitated through the use of a port which extends entirely through a microfluidic substrate. Capillary forces can be used to retain the fluid within the port, and a series of samples or other fluids may be introduced through a single port by sequentially blowing the fluid out through the substrate and replacing the removed fluid with an alternate fluid, or by displacing the fluid in part with additional fluid. In another aspect, microfluidic substrates have channels which varying in cross-sectional dimension so that capillary action spreads a fluid only within a limited portion of the channel network. In yet another aspect, the introduction ports may include a multiplicity of very small channels leading from the port to a fluid channel, so as to filter out particles or other contaminants which might otherwise block the channel at the junction between the channel and the introduction port.

公开(公告)号：US07259020B2

公开(公告)日：2007-08-21

申请号：US10208297

申请日：2002-07-30

申请人： Steven A. Sundberg ,  J. Wallace Parce ,  Calvin Y. H. Chow

发明人： Steven A. Sundberg ,  J. Wallace Parce ,  Calvin Y. H. Chow

IPC分类号： G01N1/10

CPC分类号： G01N27/44791 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00826 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00853 ,  B01J2219/00891 ,  B01J2219/00909 ,  B01L3/0244 ,  B01L3/0262 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/502753 ,  B01L2200/027 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0487 ,  B01L2400/0688 ,  G01N27/44743 ,  G01N2035/00237 ,  G01N2035/1037 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/2575

摘要： Fluid introduction is facilitated through the use of a port which extends entirely through a microfluidic substrate. Capillary forces can be used to retain the fluid within the port, and a series of samples or other fluids may be introduced through a single port by sequentially blowing the fluid out through the substrate and replacing the removed fluid with an alternate fluid, or by displacing the fluid in part with additional fluid. In another aspect, microfluidic substrates have channels which varying in cross-sectional dimension so that capillary action spreads a fluid only within a limited portion of the channel network. In yet another aspect, the introduction ports may include a multiplicity of very small channels leading from the port to a fluid channel, so as to filter out particles or other contaminants which might otherwise block the channel at the junction between the channel and the introduction port.

摘要翻译： 通过使用完全延伸通过微流体衬底的端口来促进流体引入。 可以使用毛细管力将流体保持在端口内，并且可以通过单个端口引入一系列样品或其它流体，通过依次将流体通过基底吹出，并用替代流体代替去除的流体，或者通过置换 流体部分附加流体。 在另一方面，微流体衬底具有在横截面尺寸上变化的通道，使得毛细管作用仅在通道网络的有限部分内扩散流体。 在另一方面，引入端口可以包括从端口引导到流体通道的多个非常小的通道，以便过滤除了通道和引入端口之间的连接处可能阻挡通道的颗粒或其它污染物 。

公开(公告)号：US07169277B2

公开(公告)日：2007-01-30

申请号：US09919505

申请日：2001-07-31

申请人： Walter Ausserer ,  Luc J. Bousse ,  Robert S. Dubrow ,  Steven A. Sundberg ,  Andrea W. Chow ,  Benjamin N. Wang

发明人： Walter Ausserer ,  Luc J. Bousse ,  Robert S. Dubrow ,  Steven A. Sundberg ,  Andrea W. Chow ,  Benjamin N. Wang

IPC分类号： G01N27/26

CPC分类号： G01N27/44791 ,  G01N27/44743

摘要： Devices, systems and methods for use in separating sample materials into different fractions that employ bulk fluid flow for loading of samples followed by electrophoretic separation of the sample material. Devices employ configurations that optionally allow bulk sample loading with some or no displacement of a separation matrix within a separation conduit. Methods of using these devices, and systems that incorporate these devices are also envisioned.

摘要翻译： 用于将样品材料分离成不同级分的装置，系统和方法，其使用大量流体流来装载样品，然后电泳分离样品材料。 设备采用可选地允许在分离管道内部分或不排出分离基质的大量样品加载的构造。 也可以设想使用这些装置的方法以及包含这些装置的系统。

公开(公告)号：US06509059B2

公开(公告)日：2003-01-21

申请号：US09963925

申请日：2001-09-25

申请人： Hua Yang ,  Steven A. Sundberg

发明人： Hua Yang ,  Steven A. Sundberg

IPC分类号： B05D722

CPC分类号： A61L31/10 ,  Y10T428/24562 ,  Y10T428/24661 ,  Y10T428/24744 ,  Y10T428/261 ,  Y10T428/31612 ,  Y10T428/31663 ,  C08L79/02

摘要： Hydrophilic protein adsorption resistant coatings for microfluidic devices are provided. Additionally, microfluidic devices and methods of manufacturing microfluidic devices that include the coatings are provided.

公开(公告)号：US06495369B1

公开(公告)日：2002-12-17

申请号：US09545241

申请日：2000-04-07

申请人： Joseph E. Kercso ,  Steven A. Sundberg ,  Jeffrey A. Wolk ,  Andrew W. Toth ,  Calvin Y. H. Chow ,  J. Wallace Parce

发明人： Joseph E. Kercso ,  Steven A. Sundberg ,  Jeffrey A. Wolk ,  Andrew W. Toth ,  Calvin Y. H. Chow ,  J. Wallace Parce

IPC分类号： G01N3502

CPC分类号： G01N35/028 ,  G01N2035/00148 ,  Y10T436/11 ,  Y10T436/113332 ,  Y10T436/119163 ,  Y10T436/25625 ,  Y10T436/2575

摘要： The invention provides improved systems, devices, and methods for analyzing a large number of sample compounds contained in standard multi-well microtiter plates or other array structures. The multi-well plates travel along a conveyor system to a test station having a microfluidic device. At the test station, each plate is removed from the conveyor and the wells of the multi-well plate are sequentially aligned with an input port of the microfluidic device. After at least a portion of each sample has been input into the microfluidic channel system, the plate is returned to the conveyor system. Pre and/or post testing stations may be disposed along the conveyor system, and the use of an X-Y-Z robotic arm and novel plate support bracket allows each of the samples in the wells to be input into the microfluidic network through a probe affixed to a microfluidic chip. A clamshell structure having a hinged lid can releasably support the chip while providing and/or accommodating the electrical, optical, structural, and other interface connections between the microfluidic device and the surrounding system.

摘要翻译： 本发明提供用于分析标准多孔微量滴定板或其它阵列结构中所含的大量样品化合物的改进的系统，装置和方法。 多孔板沿着输送系统行进到具有微流体装置的测试台。 在测试台处，每个板从输送机中取出，多孔板的孔依次与微流体装置的输入端对准。 在每个样品的至少一部分已经被输入到微流体通道系统中之后，板返回到输送系统。 可以沿输送机系统布置前后测试站，并且使用XYZ机器人臂和新的板支撑支架允许孔中的每个样品通过固定在微流体上的探针输入微流体网络 芯片。 具有铰接盖的蛤壳结构可以可释放地支撑芯片，同时提供和/或容纳微流体装置和周围系统之间的电，光学，结构和其它界面连接。

公开(公告)号：US06451188B1

公开(公告)日：2002-09-17

申请号：US09539671

申请日：2000-03-30

申请人： Steven A. Sundberg ,  J. Wallace Parce ,  Calvin Y. H. Chow

发明人： Steven A. Sundberg ,  J. Wallace Parce ,  Calvin Y. H. Chow

IPC分类号： G01N2726

CPC分类号： G01N27/44791 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00826 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00853 ,  B01J2219/00891 ,  B01J2219/00909 ,  B01L3/0244 ,  B01L3/0262 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/502753 ,  B01L2200/027 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0487 ,  B01L2400/0688 ,  G01N27/44743 ,  G01N2035/00237 ,  G01N2035/1037 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/2575

摘要： Fluid introduction is facilitated through the use of a port which extends entirely through a microfluidic substrate. Capillary forces can be used to retain the fluid within the port, and a series of samples or other fluids may be introduced through a single port by sequentially blowing the fluid out through the substrate and replacing the removed fluid with an alternate fluid, or by displacing the fluid in part with additional fluid. In another aspect, microfluidic substrates have channels which varying in cross-sectional dimension so that capillary action spreads a fluid only within a limited portion of the channel network. In yet another aspect, the introduction ports may include a multiplicity of very small channels leading from the port to a fluid channel, so as to filter out particles or other contaminants which might otherwise block the channel at the junction between the channel and the introduction port.

摘要翻译： 通过使用完全延伸通过微流体衬底的端口来促进流体引入。 可以使用毛细管力将流体保持在端口内，并且可以通过单个端口引入一系列样品或其它流体，通过依次将流体通过基底吹出，并用替代流体代替去除的流体，或者通过置换 流体部分附加流体。 在另一方面，微流体衬底具有在横截面尺寸上变化的通道，使得毛细管作用仅在通道网络的有限部分内扩散流体。 在另一方面，引入端口可以包括从端口引导到流体通道的多个非常小的通道，以便过滤除了通道和引入端口之间的连接处可能阻挡通道的颗粒或其它污染物 。