公开(公告)号：US10092679B2

公开(公告)日：2018-10-09

申请号：US14518352

申请日：2014-10-20

Applicant: Wake Forest University Health Sciences

Inventor： Hyunhee Ahn ,  Young Min Ju ,  Anthony Atala ,  Sang Jin Lee

IPC: A61L27/50 ,  A61L27/26 ,  A61F2/07 ,  A61L27/38 ,  A61F2/06 ,  C12N5/071 ,  D01D5/00

Abstract: Vascular scaffolds and methods of fabricating the same are disclosed for tissue engineering of vascular constructs. By combining electrospun matrices with cell sheet technologies, vascular constructs with more mature cell layers can be obtained for reconstruction of blood vessels, heart valves and the like. A engineered smooth muscle cell sheet, wrapped around an electrospun vascular scaffold, is able to provide a mature SMC layer that expresses strong cell-to-cell junction markers and contractile proteins. In addition, preconditioning of the cell sheet covered vascular scaffold maintained cell viability and infiltration into the scaffold.

公开(公告)号：US09801713B2

公开(公告)日：2017-10-31

申请号：US14987601

申请日：2016-01-04

Applicant: Wake Forest University Health Sciences

Inventor： Anthony Atala ,  James Yoo

IPC: C12N5/00 ,  A61F2/24 ,  C12N5/071 ,  A61L27/36 ,  A61L27/38 ,  A61L27/50 ,  B82Y5/00 ,  B82Y10/00

CPC classification number: A61F2/2415 ,  A61L27/3604 ,  A61L27/3641 ,  A61L27/3808 ,  A61L27/3826 ,  A61L27/383 ,  A61L27/3839 ,  A61L27/3886 ,  A61L27/507 ,  B82Y5/00 ,  B82Y10/00 ,  C12N5/0068 ,  C12N5/069 ,  C12N2533/90

Abstract: The invention is directed to methods for preparing artificial heart valves by preconditioning a matrix seeded with endothelial cells and smooth muscle cells differentiated from isolated progenitor cells. These cell seeded matrices are exposed to fluid conditions that mimic blood flow through the heart to produce tissue engineered heart valves that are analogous to native heart valves.

公开(公告)号：US09757225B2

公开(公告)日：2017-09-12

申请号：US15298382

申请日：2016-10-20

Applicant: Wake Forest University Health Sciences

Inventor： James Yoo ,  Joel D. Stitzel, Jr. ,  Anthony Atala ,  George Christ

IPC: A61F2/08 ,  A61L27/38 ,  C12M3/00 ,  C12M1/42 ,  C12N5/077 ,  A61L27/36

CPC classification number: A61F2/08 ,  A61F2002/0894 ,  A61L27/3604 ,  A61L27/367 ,  A61L27/3683 ,  A61L27/3691 ,  A61L27/3826 ,  A61L27/3895 ,  A61L2430/30 ,  A61L2430/34 ,  C12M21/08 ,  C12M35/04 ,  C12N5/0658 ,  C12N2527/00 ,  C12N2539/00

Abstract: A method of producing organized skeletal muscle tissue from precursor muscle cells in vitro comprises: (a) providing precursor muscle cells on a support in a tissue media; then (b) cyclically stretching and relaxing the support at least twice along a first axis during a first time period; and then (c) optionally but preferably maintaining the support in a substantially static position during a second time period; and then (d) repeating steps (b) and (c) for a number of times sufficient to enhance the functionality of the tissue formed on the support and/or produce organized skeletal muscle tissue on the solid support from the precursor muscle cells.

公开(公告)号：US20170107483A1

公开(公告)日：2017-04-20

申请号：US15294154

申请日：2016-10-14

Applicant: Wake Forest University Health Sciences

Inventor： Samuel Pendergraft ,  Hooman Sadri-Ardekani ,  Anthony Atala ,  Colin Bishop

IPC: C12N5/076 ,  A01N1/02

CPC classification number: A01N1/021 ,  A01N1/0221 ,  C12N5/0697 ,  C12N2506/246 ,  C12N2510/04 ,  C12N2533/90

Abstract: A cell composition composed of spermatogonial stem cells, Sertoli cells, Leydig cells and optionally peritubular cells, is provided, as is a culture composition, artificial testicular construct, hydrogel composition, and device containing the same. A method for using the device as a physiologically relevant in vitro model of human testicular function to screen compounds for pharmacological or toxicological activity is also provided.

公开(公告)号：US20250154471A1

公开(公告)日：2025-05-15

申请号：US18741884

申请日：2024-06-13

Applicant: Wake Forest University Health Sciences

Inventor： Anthony Atala ,  James J. Yoo

IPC: C12N5/071 ,  A61K9/00 ,  A61K35/12 ,  A61K35/22 ,  G01N15/01 ,  G01N15/10 ,  G01N15/1031 ,  G01N33/569

Abstract: Provided herein are isolated populations of kidney cells harvested from differentiated cells of the kidney, wherein cells have been expanded in vitro. The kidney cells may include peritubular interstitial cells of the kidney, and preferably produce erythropoietin (EPO). The kidney cells may also be selected based upon EPO production. Methods of producing an isolated population of EPO producing cells are also provided, and methods of treating a kidney disease resulting in decreased EPO production in a patient in need thereof are provided, including administering the population to the patient, whereby the cells produce EPO in vivo.

公开(公告)号：US12268467B2

公开(公告)日：2025-04-08

申请号：US16694017

申请日：2019-11-25

Applicant: Wake Forest University Health Sciences

Inventor： James J. Yoo ,  Anthony Atala ,  Kyle W. Binder ,  Weixin Zhao ,  Dennis Dice ,  Tao Xu

IPC: A61B5/00 ,  A61M35/00 ,  B41J3/407 ,  A61B17/00 ,  A61B17/322 ,  A61B34/10

Abstract: Provided herein is a delivery system, including: (a) an optical sensor configured to detect data useful to create a map of a bodily surface; and (b) a printer operatively associated with the optical sensor and configured to deliver compositions (optionally including cells) to the bodily surface based upon the data or map. Methods of forming a tissue on a bodily surface of a patient in need thereof are also provided, as are methods, systems and computer program products useful for processing bodily surface data.

公开(公告)号：US20240066067A1

公开(公告)日：2024-02-29

申请号：US18129399

申请日：2023-03-31

Applicant: Wake Forest University Health Sciences ,  Emory University ,  Children's Healthcare of Atlanta, Inc.

Inventor： Maria Graca N.D. Almeida-Porada ,  Christopher D. Porada ,  Anthony Atala ,  Christopher B. Doering ,  H. Trent Spencer

IPC: A61K35/28 ,  C07K14/755 ,  C12N5/0775

CPC classification number: A61K35/28 ,  C07K14/755 ,  C12N5/0668

Abstract: Disclosed herein are method of treating hemophilia A in a subject comprising injecting the subject with mesenchymal stromal/stem cells (MSC) modified to express high levels of Factor VIII protein. The MSC are injected into the subject prenatally. The modified MSC may also express high levels von Willebrand factor protein.

公开(公告)号：US11779464B2

公开(公告)日：2023-10-10

申请号：US16618926

申请日：2018-06-12

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： Phillip Kim ,  Anthony Atala ,  David James Mccoul

IPC: A61F2/24 ,  A61B34/10 ,  G16H50/50 ,  G16H30/40 ,  G06F30/20 ,  B33Y50/02 ,  B33Y80/00

CPC classification number: A61F2/2481 ,  A61B34/10 ,  G06F30/20 ,  G16H30/40 ,  G16H50/50 ,  A61B2034/102 ,  A61B2034/105 ,  A61B2034/108 ,  A61F2240/002 ,  A61F2250/0097 ,  B33Y50/02 ,  B33Y80/00

Abstract: Provided herein is a method of forming a ventricular support device for a diseased heart, including providing imaging data of the diseased heart, forming a three-dimensional (3D) heart model based on the imaging data, providing strain data including a plurality of strain estimates for at least one segment of the diseased heart, mapping the plurality of strain estimates onto corresponding portions of the 3D heart model to form a 3D diseased heart model, based on the 3D diseased heart model, forming a model of the ventricular support device configured to surround at least a portion of the diseased heart and provide support based upon said strain estimates, and converting the model of the ventricular support device to a digital file useful for directing a 3D printer device to print said ventricular support device for said diseased heart. Also provided is a customized ventricular support device so produced.

公开(公告)号：US11305038B2

公开(公告)日：2022-04-19

申请号：US15747352

申请日：2016-07-21

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： James Yoo ,  In Kap Ko ,  Jennifer Huling ,  Anthony Atala

IPC: A61L27/56 ,  A61K35/44 ,  A61L27/24 ,  A61L27/38 ,  A61L27/50 ,  A61L27/52 ,  A61K35/33 ,  A61K35/34 ,  A61K35/39 ,  A61K35/407 ,  A61L27/20

Abstract: The present application relates to biomimetic three-dimensional (3D) scaffolds, constructs and methods of making the same. The three-dimensional scaffold can include a sacrificial internal cast and a durable external scaffold material, wherein the durable external scaffold material comprises a biocompatible material which completely surrounds the sacrificial internal cast and wherein the sacrificial internal cast be removed to yield a branching 3D network of hollow, vessel-like tubes that substantially mimics a native tissue or organ.

公开(公告)号：US20220016179A1

公开(公告)日：2022-01-20

申请号：US17186278

申请日：2021-02-26

Applicant: Wake Forest University Health Sciences

Inventor： Sean V. Murphy ,  Aleksander Skardal ,  Anthony Atala

IPC: A61K35/50 ,  C12N5/00 ,  A61L27/36 ,  A61L27/34 ,  A61L27/48 ,  A61L27/52 ,  A61L15/40 ,  A61L26/00 ,  A61K47/36 ,  A61K47/42

Abstract: The present invention provides compositions and methods for wound healing and tissue regeneration. The compositions of the present invention comprise amniotic membrane of the placenta. In certain embodiments, the composition comprises amniotic membrane powder or solubilized amniotic membrane (SAM). In some aspects, the composition is cell-free and rich in cytokines, extracellular matrix proteins, and other components that improve tissue regeneration. In one aspect, the composition is a hydrogel scaffold that comprises amniotic membrane. The present invention reduces contraction and improves blood vessel development in regenerating tissue.