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公开(公告)号：US09764516B2
公开(公告)日：2017-09-19
申请号：US14476359
申请日：2014-09-03
发明人： Hyun-Wook Kang , Sang Jin Lee , Anthony Atala , James J. Yoo
IPC分类号： C12N5/00 , C12N5/02 , A61F13/00 , B29C67/00 , A61L27/20 , A61L27/22 , A61L27/38 , A61L27/52 , A61L27/26 , A61K35/12 , B33Y10/00 , B33Y30/00 , B33Y70/00 , B33Y80/00 , B29K105/00 , B29L31/00 , C12M3/00 , C12M1/00 , C12M1/26
CPC分类号： B29C64/112 , A61K35/12 , A61L27/20 , A61L27/222 , A61L27/225 , A61L27/26 , A61L27/38 , A61L27/52 , B29C64/124 , B29C64/386 , B29C64/393 , B29K2105/0005 , B29K2267/04 , B29L2031/7532 , B33Y10/00 , B33Y30/00 , B33Y70/00 , B33Y80/00 , C12M21/08 , C12M23/50 , C12M33/00 , C08L5/08
摘要： A method of making an organ or tissue comprises: (a) providing a first dispenser containing a structural support polymer and a second dispenser containing a live cell-containing composition; (b) depositing a layer on said support from said first and second dispenser, said layer comprising a structural support polymer and said cell-containing composition; and then (c) iteratively repeating said depositing step a plurality of times to form a plurality of layers one on another, with separate and discrete regions in each of said layers comprising one or the other of said support polymer or said cell-containing composition, to thereby produce provide a composite three dimensional structure containing both structural support regions and cell-containing regions. Apparatus for carrying out the method and composite products produced by the method are also described.
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公开(公告)号：US09763986B2
公开(公告)日：2017-09-19
申请号：US15014536
申请日：2016-02-03
IPC分类号： C12N5/07 , C12N5/00 , C12N5/02 , C12N5/071 , A61K35/54 , A61K31/565 , A61K31/57 , A61K9/16 , A61K9/50
CPC分类号： A61K35/54 , A61K9/167 , A61K9/5036 , A61K9/5073 , A61K31/565 , A61K31/57 , A61K2300/00
摘要： A composition comprising microcapsules, the microcapsules containing both live mammalian ovarian granulosa cells and live mammalian ovarian theca cells, is described. In some embodiments, the granulosa cells and the theca cells are contained in separate microcapsules in the composition; in some embodiments, the granulosa cells and the theca cells are contained together in the same microcapsules in the composition The composition is can be used for estrogen, and optionally also progesterone, delivery, and hence is preferably free or essentially free of oocytes. Methods of using the same and pharmaceutical formulations containing the same are also described.
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公开(公告)号：US09713501B2
公开(公告)日：2017-07-25
申请号：US14325908
申请日：2014-07-08
发明人： Rohan A. Shirwaiker , Caroline Elise Webster , John D. Jackson , Richard A. Wysk , Sang Jin Lee , Ola L. A. Harrysson , James J. Yoo , Anthony Atala , Paul H. Cohen , Yuan-Shin Lee , Peter M. Prim , Molly F. Purser , Katie L. Basinger
IPC分类号： A61B17/322 , A61B19/00 , A61B90/00
CPC分类号： A61B90/02 , A61B17/322 , A61B2017/3225
摘要： Provided herein is a tissue stretching device configured for orbicular expansion of a tissue placed therein. Methods of use of the device to stretch a tissue as well as for culturing organized tissues are also provided. Stretched and/or cultured tissues produced by these processes are also provided, as well as methods making use of the same.
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公开(公告)号：US20150299730A1
公开(公告)日：2015-10-22
申请号：US14697704
申请日：2015-04-28
发明人： Tao Xu , James J. Yoo , Anthony Atala
IPC分类号： C12N15/89 , A61K31/713
CPC分类号： C12N15/895 , A61K31/713 , C12N15/63 , C12N15/87 , C12N15/89
摘要： Provided herein are methods and apparatuses for transfecting a cell with a compound of interest by stressing the cell, e.g. with shear stress. The compound of interest may be nucleic acids, proteins, molecules, nanoparticles, drugs, etc., or any combination thereof. Methods of printing cells with an inkjet printing device are also provided, wherein at least a portion of viable cells (preferably at least 1%) are transfected with a compound of interest. Preferably, at least 25% of the cells are viable after printing. In addition, methods of forming an array of viable cells are provided wherein at least a portion of the viable printed cells (preferably at least 1%) are transfected with at least one compound of interest.
摘要翻译： 本文提供了用于通过施加细胞来转染具有感兴趣的化合物的细胞的方法和装置，例如， 具有剪切应力。 感兴趣的化合物可以是核酸，蛋白质，分子，纳米颗粒，药物等，或其任何组合。 还提供了用喷墨印刷装置印刷细胞的方法，其中至少一部分活细胞（优选至少1％）用感兴趣的化合物转染。 优选地，至少25％的细胞在印刷后是活的。 此外，提供形成活细胞阵列的方法，其中至少一部分活的印刷细胞（优选至少1％）用至少一种目标化合物转染。
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公开(公告)号：US20210252188A1
公开(公告)日：2021-08-19
申请号：US17237116
申请日：2021-04-22
发明人： Hyun-Wook Kang , Sang Jin Lee , Anthony Atala , James J. Yoo
IPC分类号： A61L27/20 , B29C64/393 , B29C64/112 , A61L27/22 , A61L27/38 , A61L27/52 , B29C64/124 , A61L27/26
摘要： A method of making an organ or tissue comprises: (a) providing a first dispenser containing a structural support polymer and a second dispenser containing a live cell-containing composition; (b) depositing a layer on said support from said first and second dispenser, said layer comprising a structural support polymer and said cell-containing composition; and then (c) iteratively repeating said depositing step a plurality of times to form a plurality of layers one on another, with separate and discrete regions in each of said layers comprising one or the other of said support polymer or said cell-containing composition, to thereby produce provide a composite three dimensional structure containing both structural support regions and cell-containing regions. Apparatus for carrying out the method and composite products produced by the method are also described.
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公开(公告)号：US20200345899A1
公开(公告)日：2020-11-05
申请号：US16947053
申请日：2020-07-16
发明人： Anthony Atala , Gayoung Jeong , James J. Yoo , Sang Jin Lee , Young-Joon Seol
IPC分类号： A61L27/60 , A61F2/10 , B33Y80/00 , B33Y70/00 , C08L5/08 , A61L27/20 , C08L89/06 , C12N5/071 , B33Y10/00 , A61L27/18 , A61L27/38 , A61L27/52 , G01N33/50
摘要： Provided are live, artificial, skin substitute products and methods of making and using the same, such as for wound treatment and compound testing, including compound testing for efficacy, toxicity, penetration, irritation and/or metabolism testing of drug candidates or compositions such as cosmetics.
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公开(公告)号：US10537689B2
公开(公告)日：2020-01-21
申请号：US16158808
申请日：2018-10-12
发明人： James J. Yoo , Anthony Atala , Kyle W. Binder , Mohammad Z. Albanna , Weixin Zhao , Dennis Dice , Tao Xu
IPC分类号： A61M5/178 , A61M11/00 , A61B5/00 , A61B5/107 , A61M35/00 , A61L27/22 , A61L27/24 , A61L27/38 , A61L27/60 , C12N5/077 , A61M1/00 , A61M5/00 , A61M5/315 , A61M27/00 , B41J3/407 , A61B17/00 , A61B17/322 , A61B90/50 , A61B34/30 , A61B34/10
摘要： Provided herein is a delivery system, including: (a) an optical sensor configured to detect data to create a map of a patient bodily surface; and (b) a dispenser operatively associated with the optical sensor and configured to deliver compositions (optionally including cells) to the patient bodily surface based upon the data or map. Methods of forming a tissue on a patient bodily surface of a patient in need thereof are also provided, as are methods, systems and computer program products useful for processing patient bodily surface data.
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公开(公告)号：US10398739B2
公开(公告)日：2019-09-03
申请号：US14909240
申请日：2014-09-02
摘要： A composition comprising microcapsules, the microcapsules containing both live mammalian ovarian granulosa cells and live mammalian ovarian theca cells, is described. In some embodiments, the granulosa cells and the theca cells are contained in separate microcapsules in the composition; in some embodiments, the granulosa cells and the theca cells are contained together in the same microcapsules in the composition. The composition is can be used for estrogen, and optionally also progesterone, delivery, and hence is preferably free or essentially free of oocytes. Methods of using the same and pharmaceutical formulations containing the same are also described.
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公开(公告)号：US20180339084A1
公开(公告)日：2018-11-29
申请号：US15899463
申请日：2018-02-20
申请人： Trustees of Tufts College , University of Pittsburgh - of the Commonwealth System of Higher Education , Wake Forest University Health Sciences
发明人： Evangelia Bellas , Kacey Marra , J. Peter Rubin , David L. Kaplan , James J. Yoo
IPC分类号： A61L27/22 , A61L27/56 , A61L27/54 , A61L27/58 , A61L27/48 , A61L27/38 , A61L27/36 , C08L89/00
摘要： The inventions provided herein relate to compositions, methods, delivery devices and kits for repairing or augmenting a tissue in a subject. The compositions described herein are injectable such that they can be placed in a tissue to be treated with a minimally-invasive procedure (e.g., by injection) and/or be molded flexibly into a tissue void of any shape and/or size. In some embodiments, the composition described herein comprises a plurality of silk fibroin particles, which can retain their original volume within the tissue for a period of time. The compositions can be used as a filler to replace a tissue void, e.g., for tissue repair and/or augmentation, or as a scaffold to support tissue regeneration and/or reconstruction. In some embodiments, the compositions described herein can be used for soft tissue repair or augmentation.
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公开(公告)号：US10118005B2
公开(公告)日：2018-11-06
申请号：US14019714
申请日：2013-09-06
发明人： James J. Yoo , Anthony Atala , Kyle W. Binder , Mohammad Z. Albanna , Weixin Zhao , Dennis Dice , Tao Xu
IPC分类号： A61M11/00 , A61B5/00 , A61B5/107 , A61M35/00 , A61L27/22 , A61L27/24 , A61L27/38 , A61L27/60 , C12N5/077 , B41J3/407 , A61B17/00 , A61B17/322 , A61B90/50 , A61B34/30 , A61B34/10
摘要： Provided herein is a delivery system, including: (a) an optical sensor configured to detect data to create a map of a patient bodily surface; and (b) a dispenser operatively associated with the optical sensor and configured to deliver compositions (optionally including cells) to the patient bodily surface based upon the data or map. Methods of forming a tissue on a patient bodily surface of a patient in need thereof are also provided, as are methods, systems and computer program products useful for processing patient bodily surface data.
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