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    MICROFLUIDIC DEVICES AND METHODS FOR CELL PROCESSING
    22.
    发明申请
    MICROFLUIDIC DEVICES AND METHODS FOR CELL PROCESSING 审中-公开
    微流体装置和细胞处理方法

    公开(公告)号：US20160167051A1

    公开(公告)日：2016-06-16

    申请号：US14808703

    申请日：2015-07-24

    申请人： John Collins

    发明人： John Collins

    IPC分类号： B01L3/00 ,  G01N33/483 ,  C12N5/074

    CPC分类号： B01L3/502761 ,  B01L3/502753 ,  B01L7/52 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2300/0832 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0883 ,  B01L2300/1822 ,  B01L2400/086 ,  C12N5/0696 ,  G01N15/1031 ,  G01N15/1056 ,  G01N33/4833

    摘要： Microfluidic devices and methods that use cells such as cancer cells, stem cells, blood cells for preprocessing, sorting for various biodiagnostics or therapeutical applications are described. Microfluidics electrical sensing such as measurement of field potential or current and phenomena such as immiscible fluidics, inertial fluidics are used as the basis for cell and molecular processing (e.g., characterizing, sorting, isolation, processing, amplification.) of different particles, chemical compositions or biospecies (e.g., different cells, cells containing different substances, different particles, different biochemical compositions, proteins, enzymes etc.). Specifically, the present invention discloses a number of sorting schemes for stem cells, whole blood and circulating tumor cells, and extracting serum from whole blood.

    摘要翻译： 描述了使用诸如癌细胞，干细胞，用于预处理的血细胞，用于各种生物诊断或治疗应用的分选的细胞的微流体装置和方法。 微流体电传感如测量场电位或电流等现象如不混溶流体，惯性流体作为细胞和分子加工（如表征，分选，分离，加工，扩增）的基础，不同颗粒，化学成分 或生物种群（例如，不同的细胞，含有不同物质的细胞，不同的颗粒，不同的生物化学成分，蛋白质，酶等）。 具体地说，本发明公开了一些干细胞，全血和循环肿瘤细胞的分选方案，并从全血中提取血清。

    APPARATUS FOR ELECTRICALLY MEASURING INDIVIDUAL PARTICLES FLOWING IN A LIQUID
    25.
    发明申请
    APPARATUS FOR ELECTRICALLY MEASURING INDIVIDUAL PARTICLES FLOWING IN A LIQUID 审中-公开
    用于电测量在液体中流动的个体颗粒的装置

    公开(公告)号：US20160041081A1

    公开(公告)日：2016-02-11

    申请号：US14392104

    申请日：2014-04-15

    申请人： UNIVERSITY OF SOUTHAMPTON

    发明人： Daniel Christopher Spencer ,  Hywel Morgan

    IPC分类号： G01N15/10

    CPC分类号： G01N15/1031 ,  G01N15/1056 ,  G01N2015/1254

    摘要： Apparatus (3) for electrically measuring individual particles (4) flowing in a liquid (6), which apparatus (3) comprises: (i) a fluidic channel (5) for receiving a liquid (6) having the individual particles (4) in suspension in the liquid (6); (ii) a first electrode arrangement (8) having at least one measurement electrode (16) and at least one signal electrode (11); (iii) at least one other electrode arrangement (9) having at least one measurement electrode and at least one signal electrode (13); (iv) at least one signal conditioning electrode (10, 12, 14, 15, 17, 19) positioned adjacent to at least one of the measurement electrodes (16, 18) or at least one of the signal electrode (9); and (v) measuring means (20, 21) for measuring electrical signal changes; and the apparatus (3) being such that: (vi) the first and the other electrode arrangements (8, 9) are connected to the measuring means (20, 21) whereby individual particles passing between the first and other electrode arrangements (8, 9) cause a change in electrical signal which is measured; and (vii) the electrical potential of the signal conditioning electrode (10, 12, 14, 15, 17, 19) is controlled to substantially prevent current flow between the first electrode arrangement (8) and the other electrode arrangement (9).

    摘要翻译： 用于电测量在液体（6）中流动的单个颗粒（4）的装置（3），该装置（3）包括：（i）用于接收具有单个颗粒（4）的液体（6）的流体通道（5） 悬浮在液体（6）中; （ii）具有至少一个测量电极（16）和至少一个信号电极（11）的第一电极装置（8）; （iii）至少一个具有至少一个测量电极和至少一个信号电极（13）的其它电极装置（9）; （iv）至少一个位于与所述测量电极（16,18）中的至少一个或所述信号电极（9）中的至少一个相邻的信号调节电极（10,12,14,15,17,19）。 和（v）用于测量电信号变化的测量装置（20,21）; 并且所述装置（3）使得：（vi）所述第一和第二电极装置（8,9）连接到所述测量装置（20,21），由此通过所述第一和其它电极装置（8， 9）导致测量的电信号的变化; 和（vii）信号调节电极（10,12,14,15,17,19）的电位被控制以基本上防止第一电极装置（8）和另一个电极装置（9）之间的电流流动。

    SYSTEMS, DEVICES AND METHODS FOR TRANSLOCATION CONTROL
    26.
    发明申请
    SYSTEMS, DEVICES AND METHODS FOR TRANSLOCATION CONTROL 审中-公开
    用于转移控制的系统，设备和方法

    公开(公告)号：US20160025702A1

    公开(公告)日：2016-01-28

    申请号：US14775360

    申请日：2014-03-12

    申请人： ARIZONA BOARD OF REGENTS on behalf of Arizona STATE UNIVERSITY

    发明人： Stuart LINDSAY ,  Brett GYARFAS ,  Predrag KRSTIC ,  Padmini KRISHNAKUMAR

    IPC分类号： G01N33/487

    CPC分类号： G01N33/48721 ,  C12Q1/6869 ,  G01N15/1031 ,  G01N15/1056 ,  G01N2015/0038 ,  C12Q2565/631

    摘要： Some embodiments of the present disclosure are directed to systems, methods and devices for controlling the transit of a molecule across a nanopore. Some embodiments are directed to a device comprising a first compartment, a second compartment, a first pair of electrodes comprising a first electrode provided in the first compartment and a second electrode provided in the second compartment, a partition separating the first compartment from the second compartment, an orifice provided in the partition, a second pair of electrodes arranged proximate the orifice, the second pair of electrodes being functionalized with molecules, and a tunnel gap comprising the spacing between the second pair of electrodes.

    摘要翻译： 本公开的一些实施方案涉及用于控制分子跨越纳米孔的转运的系统，方法和装置。 一些实施例涉及包括第一隔室，第二隔室，包括设置在第一隔室中的第一电极和设置在第二隔室中的第二电极的第一对电极的设备，将第一隔室与第二隔室分开的隔板 ，设置在隔板中的孔口，邻近孔口布置的第二对电极，第二对电极用分子功能化，以及包括第二对电极间的间隔的隧道间隙。

    MEASUREMENT APPARATUS AND MEASUREMENT METHOD
    27.
    发明申请
    MEASUREMENT APPARATUS AND MEASUREMENT METHOD 审中-公开
    测量装置和测量方法

    公开(公告)号：US20160025610A1

    公开(公告)日：2016-01-28

    申请号：US14775099

    申请日：2014-02-07

    申请人： SONY CORPORATION

    发明人： Yoichi Katsumoto ,  Kazumasa Sato ,  Marcaurele Brun

    IPC分类号： G01N15/12 ,  G01N15/02

    CPC分类号： G01N15/1209 ,  G01N15/0266 ,  G01N15/1031 ,  G01N15/1056 ,  G01N15/1227 ,  G01N2015/0065 ,  G01N2015/1081

    摘要： A measurement apparatus includes a generation section and a measurement section and performs a predetermined measurement using a flow channel device including a narrow channel through which particles pass by a flow of a fluid and a measurement electrode section provided in the vicinity of the narrow channel. The generation section generates, in the measurement electrode section, an AC voltage that is higher than a characteristic frequency defined by a conductance of the fluid including the particles in the narrow channel and an electrical double layer capacitance formed by the measurement electrode section and has a frequency range indicating a conductance corresponding to a size of the particles. The measurement section measures, while the AC voltage is applied to the measurement electrode section, an electrical amount including at least the conductance obtained when the particles pass through the narrow channel.

    摘要翻译： 测量装置包括生成部分和测量部分，并且使用包括通过流体的微粒通过的窄通道的流道装置和设置在窄通道附近的测量电极部分的流路装置进行预定的测量。 该生成部在测量电极部中产生高于由窄通道中包含粒子的流体的导电性所决定的特性频率的AC电压和由测量电极部形成的双电层电容， 频率范围表示对应于颗粒尺寸的电导。 测量部分测量交流电压被施加到测量电极部分时，至少包括当粒子通过窄通道时获得的电导的电量。

    Apparatus for sample introduction, chip for sample introduction, and method for sample introduction
    30.
    发明授权
    Apparatus for sample introduction, chip for sample introduction, and method for sample introduction 有权
    样品引入装置，样品引入芯片和样品引入方法

    公开(公告)号：US09211548B2

    公开(公告)日：2015-12-15

    申请号：US13277699

    申请日：2011-10-20

    申请人： Yoichi Katsumoto

    发明人： Yoichi Katsumoto

    IPC分类号： G01N27/447 ,  G01N27/26 ,  B03C5/00 ,  B03C5/02 ,  G01N15/10

    CPC分类号： B03C5/005 ,  B03C5/026 ,  G01N15/1031 ,  G01N15/1056

    摘要： The present disclosure provides an apparatus for sample introduction including a flow channel, a sample inlet, a metering unit, a separating unit, cell dischargers and, and a pressure controlling unit. The sample inlet is given a sample fluid such as blood. The sample inlet has a constricting hole that communicates with the flow channel for passage of a carrier fluid. The constricting hole has a diameter large enough for passage of a single cell contained in the sample fluid. The sample fluid is introduced into the flow channel through the constricting hole, and the cells in the sample fluid pass one by one through the constricting hole. The constricting hole does not exist inside the flow channel for passage of the carrier fluid, so that the diameter of the constricting hole does not affect the flow rate of the carrier fluid passing through the flow channel.

    摘要翻译： 本公开提供了一种用于样品引入的装置，包括流动通道，样品入口，计量单元，分离单元，电池放电器和压力控制单元。 样品入口被给予样品流体，如血液。 样品入口具有与流动通道连通的收缩孔，用于载运流体的通过。 收缩孔具有足够大的直径以容纳在样品流体中的单个细胞的通过。 将样品流体通过收缩孔引入流动通道，并且样品流体中的细胞通过收缩孔逐个通过。 收缩孔不存在于用于载体流体通过的流动通道内，使得收缩孔的直径不影响通过流动通道的载体流体的流量。