公开(公告)号：US20180258415A1

公开(公告)日：2018-09-13

申请号：US15907845

申请日：2018-02-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Theo NIKIFOROV

IPC: C12Q1/6869 ,  C12N9/12 ,  C12N9/96

CPC classification number: C12Q1/6869 ,  C07H19/20 ,  C12N9/1241 ,  C12N9/1252 ,  C12N9/96 ,  C12Q1/6818 ,  C12Y207/07 ,  C12Y207/07007 ,  G01N21/6428 ,  G01N33/582 ,  G01N2021/6432

Abstract: Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are labeled polymerase conjugates including a polymerase linked to one or more labels, wherein the conjugate has polymerase activity. Such conjugates can exhibit enhanced biological activity and/or superior detectability as compared to conventional labeled polymerases. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.

公开(公告)号：US10072298B2

公开(公告)日：2018-09-11

申请号：US14672066

申请日：2015-03-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Daniel Rhodes ,  Seth Sadis ,  Peter Wyngaard ,  Nikolay Khazanov ,  Santhoshi Bandla ,  Mark Tomilo ,  Sean Eddy ,  Emma Bowden ,  Jia Li

IPC: C12Q1/6886 ,  G06F19/18 ,  G06F19/22 ,  G06F19/24

CPC classification number: C12Q1/6886 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  G16B20/00 ,  G16B30/00 ,  G16B40/00

Abstract: The disclosure provides gene fusions, gene variants, and novel associations with disease states, as well as kits, probes, and methods of using the same.

公开(公告)号：US10066260B2

公开(公告)日：2018-09-04

申请号：US14774662

申请日：2014-03-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David Light ,  Wolfgang Hinz ,  Ronald L. Cicero ,  Christina E. Inman ,  Paul M. Kenney ,  Alexander Mastroianni ,  Roman Rozhkov ,  Yufang Wang ,  Jeremy Gray ,  Marc Glazer ,  Dmitriy Gremyachinskiy

IPC: C12Q1/6837 ,  C12Q1/6806 ,  C12Q1/6869

CPC classification number: C12Q1/6837 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6869 ,  C12Q2523/101 ,  C12Q2527/119 ,  C12Q2535/122 ,  C12Q2547/107 ,  C12Q2563/159 ,  C12Q2565/537 ,  C12Q2523/301 ,  C12Q2523/32

Abstract: A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.

公开(公告)号：US20180245134A1

公开(公告)日：2018-08-30

申请号：US15965595

申请日：2018-04-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： John LEAMON ,  Mark ANDERSEN ,  Michael THORNTON

IPC: C12Q1/686 ,  C12N15/10 ,  C12Q1/6855 ,  C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6886

CPC classification number: C12Q1/686 ,  C12N15/1065 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6874 ,  C12Q1/6886 ,  C12Q2600/16 ,  C12Q2521/307 ,  C12Q2525/101 ,  C12Q2525/191 ,  C12Q2537/143

Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

公开(公告)号：US10059987B2

公开(公告)日：2018-08-28

申请号：US15625800

申请日：2017-06-16

Applicant: Life Technologies Corporation

Inventor： John Davidson ,  Wolfgang Hinz ,  Jonathan Rothberg ,  Richard Whitaker

IPC: C12N9/12 ,  C12Q1/6869 ,  G01N27/414

CPC classification number: C12Q1/6869 ,  C12N9/1252 ,  C12Y207/07007 ,  G01N27/4145

Abstract: Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.

公开(公告)号：US20180237595A1

公开(公告)日：2018-08-23

申请号：US15871863

申请日：2018-01-15

Applicant: Life Technologies AS ,  Life Technologies Corporation

Inventor： Geir FONNUM ,  Steven M. MENCHEN ,  M. Talha GOKMEN ,  Pontus LUNDBERG ,  Prasanna Krishnan THWAR ,  Alfred LUI ,  Lily LU ,  Wolfgang HINZ ,  Lene Husaboe ,  Elisabeth BREIVOLD ,  Astrid Evenroed MOLTEBERG ,  Synne LARSEN

IPC: C08J3/075 ,  C08F8/12

CPC classification number: C08J3/075 ,  C08F8/12 ,  C08F220/58 ,  C08F220/70 ,  C08F2230/085 ,  C08J2333/26 ,  C08F222/38

Abstract: A polymer substrate, such as a polymer particle, is formed from a carboxyl functional monomer. In an example, the carboxyl functional monomer has a protection group in place of the OH of the carboxyl group. Once the monomer is polymerized, such a protection group can be removed, providing a polymer network with carboxyl functional sites. Such sites can be used to attach other functionality to the polymer substrate.

公开(公告)号：US20180230516A1

公开(公告)日：2018-08-16

申请号：US15950921

申请日：2018-04-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jian GU ,  Kelli BRAMLETT ,  Christopher BURNETT

IPC: C12Q1/6806 ,  C12Q1/6848

CPC classification number: C12Q1/6806 ,  C12Q1/6848 ,  C12Q2521/501 ,  C12Q2525/113 ,  C12Q2525/186 ,  C12Q2525/191 ,  C12Q2537/163

Abstract: Disclosed are compositions and methods for the preparation of RNA libraries for sequencing, gene expression profiling, microarray and other uses and for simplification of the library preparation process. The disclosure provides blocking oligonucleotides which bind to byproduct nucleic acid molecules formed during the ligation of adapters to nucleic acid segments prior to sequencing and inhibit or block amplification of the byproduct nucleic acid molecules in subsequent amplification reactions. Methods for library preparation using blocking oligonucleotides are also provided.

公开(公告)号：US20180225415A1

公开(公告)日：2018-08-09

申请号：US15745218

申请日：2016-07-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Nivedita Sumi MAJUMDAR

IPC: G06F19/26 ,  G06F3/0481 ,  G06T11/20

Abstract: In some embodiments, a method or system for visualizing data generated from one or more reaction devices, chips, or reaction sites may be provided. On a scatter plot, data points may be displayed indicative of results from nucleic amplification, wherein the nucleic acid amplification comprises a first target and a second target and the data points are designated as being indicative of amplification of the first target, the second target, both targets, or neither target. In response to user input, an adjustable threshold may be varied used to designate the data points. And, in response to the varying, designations and a display of the designations may be altered for one or more of the data points whose designation is changed based on the varied threshold.

公开(公告)号：US20180223565A1

公开(公告)日：2018-08-09

申请号：US15890307

申请日：2018-02-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Thomas Carr ,  Karen Spinks ,  Darren Thierry ,  Matt Willmon ,  Justin Curry ,  Alex Kascoutas

IPC: E05B47/00 ,  G07F17/12 ,  G07F11/02 ,  G07F11/00 ,  G07F9/10 ,  G07F9/02 ,  G07F7/10 ,  G07F7/08 ,  G07C9/00 ,  G06Q20/32 ,  G06Q20/18 ,  E05C19/02

Abstract: Provided herein is an inventory management system for dispensing products from an enclosure, methods of securing products within the enclosure, use of the enclosure and for maintaining inventory. The inventory management system may include an enclosure having a self-locking door. The self locking door may include a door, a lock located on the door, a strike plate, wherein the strike plate controls the lock, and a sensor configured to detect the presence of a user and wherein the sensor controls the lock.

公开(公告)号：US20180223334A1

公开(公告)日：2018-08-09

申请号：US15882789

申请日：2018-01-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Parul ANGRISH ,  Zhiwei YANG

IPC: C12Q1/686 ,  C07D207/16 ,  C12N9/12 ,  C07C41/22 ,  C07C211/63 ,  C07C303/28 ,  C12N9/96 ,  C07D211/60 ,  C11D1/88

Abstract: This disclosure relates to novel compounds for use in various compositions, kits and methods, including, for example, use in polymerase storage buffers and in nucleic acid synthesis or amplification reactions such as a polymerase chain reaction (PCR). Methods for preparing the novel compounds are also described.