公开(公告)号：US12044613B2

公开(公告)日：2024-07-23

申请号：US17413706

申请日：2019-01-13

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Viktor Shkolnikov ,  Diane R. Hammerstad

IPC: G01N15/1434 ,  G01N15/00 ,  G01N15/1404

CPC classification number: G01N15/1434 ,  G01N2015/0053 ,  G01N2015/1422

Abstract: A particle classification system may include a volume to contain a fluid having a suspended particle, electrodes proximate the volume to apply an electric field to rotate the suspended particle, a focused light source, a scattered light sensor to sense light from the focused light source that has scattered upon impinging the rotating suspended particle and a controller to classify the particle based at least in part upon signals from the scattered light sensor.

公开(公告)号：US12032147B2

公开(公告)日：2024-07-09

申请号：US17255808

申请日：2018-10-01

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Fausto D'Apuzzo ,  Viktor Shkolnikov

IPC: G02B21/12 ,  G01N15/0227 ,  G01N15/10 ,  G01N15/14 ,  G01N15/1433 ,  G01N15/1434 ,  G02B21/00 ,  G02B21/36

CPC classification number: G02B21/12 ,  G01N15/0227 ,  G01N15/1433 ,  G01N15/1434 ,  G01N15/1459 ,  G02B21/0004 ,  G02B21/36 ,  G01N2015/1006 ,  G01N2015/1493

Abstract: A system and a method for imaging microfluidic ejectors during operation. An example provides a microscopy system, that includes a plurality of microfluidic ejectors. A mirror is disposed in a droplet path of the plurality of microfluidic ejectors, wherein the droplet path passes through an opening in the mirror. An optical system is focused on the plurality of microfluidic ejectors through the mirror, wherein the optical system comprises a camera.

公开(公告)号：US20240218434A1

公开(公告)日：2024-07-04

申请号：US18557281

申请日：2021-04-30

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Michael Cumbie ,  Brian J Keefe ,  Kenza Samlali ,  Viktor Shkolnikov

IPC: C12Q1/6844 ,  B01L3/00 ,  B01L7/00

CPC classification number: C12Q1/6844 ,  B01L3/502715 ,  B01L3/502792 ,  B01L7/52 ,  B01L2200/16 ,  B01L2300/0645 ,  B01L2300/0654 ,  B01L2400/0427

Abstract: A PCR system is described. The PCR system comprises a thermocycling chamber comprising an electrode assembly configured to generate an electric field and cause reversible electrowetting of a PCR mixture within the thermocycling chamber; and an optical sensor configured to obtain optical signals from the thermocycling chamber. Also described is a method of manufacturing a PCR system and a method of performing PCR.

公开(公告)号：US20240216912A1

公开(公告)日：2024-07-04

申请号：US18557567

申请日：2021-04-30

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Michael Cumbie ,  Viktor Shkolnikov ,  Chien-Hua Chen

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L3/502707 ,  B01L2200/025 ,  B01L2200/12 ,  B01L2300/0663 ,  B01L2300/12 ,  B01L2300/1805

Abstract: A microfluidic device is described. The microfluidic device comprises a reaction chamber and a temperature control module overlaying the reaction chamber. The temperature control module comprises a temperature regulating flow channel disposed between two layers and in thermal contact with the reaction chamber, and wherein at least a portion of each layer comprises an optically transparent material. Also described is a method of manufacturing a microfluidic device and a method of controlling temperature of a reaction in a microfluidic device.

公开(公告)号：US12025549B2

公开(公告)日：2024-07-02

申请号：US17414155

申请日：2019-05-13

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Viktor Shkolnikov ,  Alexander Govyadinov ,  Diane R. Hammerstad

IPC: G01N15/12 ,  B01L3/00 ,  B81B7/02 ,  G01N15/10 ,  G01N15/1031 ,  G01N33/543

CPC classification number: G01N15/12 ,  B01L3/502761 ,  B81B7/02 ,  G01N15/1023 ,  G01N15/1031 ,  G01N33/54386 ,  B01L2200/025 ,  B01L2200/0652 ,  B01L2200/0663 ,  B01L2300/0645 ,  B01L2300/0663 ,  B01L2300/0829 ,  B01L2300/14 ,  B01L2400/02 ,  B01L2400/0487 ,  G01N2015/1006 ,  G01N2015/1028

Abstract: A system includes a microchannel analysis region, a first fluid actuation device, a second fluid actuation device, a sensor, and a controller. The first fluid actuation device is at a first end of the microchannel analysis region. The second fluid actuation device is at a second end of the microchannel analysis region opposite to the first end. The sensor is within the microchannel analysis region between the first fluid actuation device and the second fluid actuation device. The sensor measures an impedance of a fluid within the microchannel analysis region. The controller activates the first fluid actuation device to generate a first pressure wave in the fluid and activates the second fluid actuation device to generate a second pressure wave in the fluid. The first pressure wave and the second pressure wave converge at the sensor.

公开(公告)号：US20240207857A1

公开(公告)日：2024-06-27

申请号：US18557285

申请日：2021-04-29

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Viktor Shkolnikov ,  Alexander Govyadinov

IPC: B01L7/00 ,  B01L3/02

CPC classification number: B01L7/52 ,  B01L3/0241 ,  B01L2200/0673 ,  B01L2200/16 ,  B01L2300/1805 ,  B01L2400/0487

Abstract: A droplet PCR system is described herein. The droplet PCR system comprises a chamber having a heating surface, a droplet dispenser to dispense PCR-reagent-containing droplets into the chamber, a heater disposed onto the heating surface of the chamber to heat a layer of fluid adjacent the heating surface, and a controller to control the heater to produce a pulsed heat flux.

公开(公告)号：US20240123446A1

公开(公告)日：2024-04-18

申请号：US18202845

申请日：2023-05-26

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Alexander Govyadinov ,  Viktor Shkolnikov

IPC: B01L3/00

CPC classification number: B01L3/502761 ,  B01L2200/025 ,  B01L2300/0829

Abstract: A microfluidic system may comprise a dispense head with multiple dispensers, each dispensing a different cell type, such as single pairs of individual target cells and individual sensor cells. Interaction between the cells may be observed based on, for example, fluorescence. Individual target cells may then be selected, based on observations, for use or for further investigation. As an example, target cells may be B-cells, and enhanced selection of B-cells aids more direct antibody discovery.

公开(公告)号：US20240024874A1

公开(公告)日：2024-01-25

申请号：US18021775

申请日：2020-08-17

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Viktor Shkolnikov ,  Alexander N. Govyadinov

IPC: B01L3/00

CPC classification number: B01L3/502761 ,  B01L2200/0668 ,  B01L2400/043 ,  B01L2300/1827

Abstract: A microfluidic magnetic microbead interaction method may include mixing ferromagnetic microbeads in a fluid while the fluid is at a first temperature above a Curie temperature of the ferromagnetic microbeads, applying a magnetic field to the ferromagnetic microbeads while the fluid is at a second temperature at or below the Curie temperature of the ferromagnetic microbeads and withdrawing remaining supernatant portions of the fluid while the magnetic field is applied to the ferromagnetic microbeads.

公开(公告)号：US20230381779A1

公开(公告)日：2023-11-30

申请号：US18032710

申请日：2020-10-20

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Alexander Govyadinov ,  Viktor Shkolnikov

IPC: B01L3/00 ,  G01N35/00 ,  B01L3/02

CPC classification number: B01L3/502761 ,  G01N35/0098 ,  B01L3/0268 ,  B01L2300/1827 ,  B01L2400/043 ,  B01L2400/0442 ,  G01N2035/00237

Abstract: A method comprising receiving, at a microfluidic channel, a biologic sample including a cell and providing a magnetic field within the microfluidic channel using a first magnet, wherein the magnetic field attracts a first plurality of magnetic particles disposed within the microfluidic channel. The method further includes activating a first resistor disposed within the microfluidic channel to agitate a volume of fluid within the microfluidic channel, and in response, moving the first plurality of magnetic particles through the microfluidic channel to lyse the cell and to release cellular material from the cell.

公开(公告)号：US11801509B2

公开(公告)日：2023-10-31

申请号：US17047353

申请日：2018-08-10

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Viktor Shkolnikov ,  Alexander Govyadinov

IPC: G01N15/10 ,  B01L3/00 ,  C12N1/06

CPC classification number: B01L3/502761 ,  C12N1/066 ,  G01N15/1031 ,  G01N15/1056 ,  B01L2200/0652 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0864 ,  B01L2400/0439 ,  B01L2400/0442 ,  G01N2015/1006 ,  G01N2015/1081

Abstract: In one example in accordance with the present disclosure, a cellular analytic system is described. The cellular analytic system includes a series of analytic devices. Each analytic device includes 1) a separator to separate a cellular particle from a surrounding fluid, 2) an analyzer coupled to a first outlet of the separator to analyze the surrounding fluid, and 3) at least one lysing device coupled to at least a second outlet of the separator to rupture a membrane of the cellular particle. An outlet of the lysing device is fluidly coupled to a separator of a downstream analytic device.