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    Automated process line
    46.
    发明授权
    Automated process line 有权
    自动化生产线

    公开(公告)号：US06730517B1

    公开(公告)日：2004-05-04

    申请号：US09680581

    申请日：2000-10-05

    Applicant: Hubert Köster ,  Ping Yip ,  Jhobe Steadman ,  Dirk Reuter ,  Richard MacDonald

    Inventor： Hubert Köster ,  Ping Yip ,  Jhobe Steadman ,  Dirk Reuter ,  Richard MacDonald

    IPC: G01N3502

    CPC classification number: G01N35/0099 ,  Y10T436/11 ,  Y10T436/113332 ,  Y10T436/203332 ,  Y10T436/24 ,  Y10T436/25875

    Abstract: Fully automated modular analytical systems with integrated instrumentation for analysis of biopolymer samples, such as nucleic acids, proteins, peptides and carbohydrates, are provided. Analytical methods of detection and analysis, such as mass spectrometry, radiolabeling, mass tags, chemical tags and fluorescence chemiluminescence, are integrated with robotic technology and automated chemical reaction systems to provide a high-throughput, accurate Automated Process Line (APL).

    Abstract translation: 提供了具有用于分析生物聚合物样品（如核酸，蛋白质，肽和碳水化合物）的集成仪器的全自动模块化分析系统。 检测和分析的分析方法，如质谱，放射性标记，质量标签，化学标签和荧光化学发光，与机器人技术和自动化学反应系统集成，提供高通量，精确的自动生产线（APL）。

    DNA diagnostics based on mass spectrometry
    47.
    发明授权
    DNA diagnostics based on mass spectrometry 失效
    基于质谱的DNA诊断

    公开(公告)号：US06602662B1

    公开(公告)日：2003-08-05

    申请号：US09724877

    申请日：2000-11-28

    Applicant: Hubert Köster ,  Daniel P. Little ,  Andreas Braun

    Inventor： Hubert Köster ,  Daniel P. Little ,  Andreas Braun

    IPC: C12Q168

    CPC classification number: C12Q1/6872 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6837 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q1/6883 ,  C12Q1/706 ,  G01N35/1067 ,  H01J49/00 ,  Y10T436/143333 ,  Y10T436/24 ,  C12Q2565/627 ,  C12Q2537/113 ,  C12Q2535/113 ,  C12Q2521/325 ,  C12Q2535/131 ,  C12Q2525/107 ,  C12Q2523/107 ,  C12Q2523/319 ,  C12Q2565/625 ,  C12Q2535/125

    Abstract: Fast and highly accurate mass spectrometry-based processes for detecting particular nucleic acid molecules and sequences in the molecules are provided. Depending upon the sequence to be detected, the processes, for example, can be used to diagnose a genetic disease or a chromosomal abnormality, a predisposition to a disease or condition, or infection by a pathogen, or for determining identity or heredity. One aspect provides a process for determining whether a target nucleotide is present in a nucleic acid molecule including hybridizing a nucleic acid molecule with a primer oligonucleotide; contacting the hybridized primer with deoxyribonucleoside triphosphates, chain terminating nucleotides and a DNA polymerase, whereby the hybridized primer is extended until a chain terminating nucleotide is incorporated, producing an extended primer, and determining the molecular mass of the extended primer, thereby determining whether the target nucleotide is present in a nucleic acid molecule.

    Abstract translation: 提供了用于检测分子中特定核酸分子和序列的基于质谱的快速和高精度的方法。 根据待检测的序列，例如，该过程可用于诊断遗传疾病或染色体异常，疾病或病症的易感性或病原体的感染，或用于确定身份或遗传。 一方面提供了确定靶核苷酸是否存在于核酸分子中的方法，包括将核酸分子与引物寡核苷酸杂交; 使杂交引物与脱氧核糖核苷三磷酸，链终止核苷酸和DNA聚合酶接触，由此将杂交引物延伸直到引入链终止核苷酸，产生延伸引物，并测定延伸引物的分子量，由此确定靶 核苷酸存在于核酸分子中。

    Solid phase sequencing of double-stranded nucleic acids
    48.
    发明授权
    Solid phase sequencing of double-stranded nucleic acids 失效
    双链核酸的固相测序

    公开(公告)号：US06436635B1

    公开(公告)日：2002-08-20

    申请号：US08614151

    申请日：1996-03-12

    Applicant: Dong-Jing Fu ,  Charles R. Cantor ,  Hubert Köster ,  Cassandra L. Smith

    Inventor： Dong-Jing Fu ,  Charles R. Cantor ,  Hubert Köster ,  Cassandra L. Smith

    IPC: C12Q168

    CPC classification number: C12Q1/6874 ,  B01J19/0046 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/0059 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00722 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6872 ,  C40B40/06 ,  C12Q2565/537 ,  C12Q2525/161 ,  C12Q2565/501 ,  C12Q2565/627

    Abstract: This invention relates to methods for detecting and sequencing of target double-stranded nucleic acid sequences, to nucleic acid probes and arrays of probes useful in these methods, and to kits and systems which contain these probes. Useful methods involve hybridizing the nucleic acids or nucleic acids which represent complementary or homologous sequences of the target to an array of nucleic acid probes. These probe comprise a single-stranded portion, an optional double-stranded portion and a variable sequence within the single-stranded portion. The molecular weights of the hybridized nucleic acids of the set can be determined by mass spectroscopy, and the sequence of the target determined from the molecular weights of the fragments. Nucleic acids whose sequences can be determined include nucleic acids in biological samples such as patient biopsies and environmental samples. Probes may be fixed to a solid support such as a hybridization chip to facilitate automated determination of molecular weights and identification of the target sequence.

    Abstract translation: 本发明涉及用于检测和测序靶双链核酸序列，用于这些方法的核酸探针和探针阵列的方法，以及含有这些探针的试剂盒和系统。 有用的方法包括将代表靶的互补或同源序列的核酸或核酸与核酸探针阵列进行杂交。 这些探针包含在单链部分内的单链部分，任选的双链部分和可变序列。 该组的杂交核酸的分子量可以通过质谱法测定，并且靶标的序列由片段的分子量确定。 可以确定其序列的核酸包括生物样品中的核酸，例如患者活组织检查和环境样品。 探针可以固定在固体支持物例如杂交芯片上以促进分子量的自动测定和靶序列的鉴定。

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