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    DROPLET-BASED AFFINITY ASSAYS
    51.
    发明申请
    DROPLET-BASED AFFINITY ASSAYS 有权
    基于DROPLET的亲和力测定

    公开(公告)号：US20130252262A1

    公开(公告)日：2013-09-26

    申请号：US13890715

    申请日：2013-05-09

    Applicant: ADVANCED LIQUID LOGIC INC.

    Inventor： Vijay Srinivasan ,  Vamsee K. Pamula ,  Michael G. Pollack ,  Richard B. Fair

    IPC: G01N27/72

    CPC classification number: G01N27/72 ,  G01N27/745 ,  G01N33/54326

    Abstract: A method of detecting a target analyte in a sample, including executing electrowetting-mediated droplet operations and thereby: combining one or more immunoassay reagent droplets comprising magnetically-responsive beads having affinity for the target analyte with one or more sample droplets potentially comprising the target analyte to yield a first combined droplet; beginning with the combined droplet, effecting a droplet-based washing protocol to wash the magnetically-responsive beads to yield a first washed droplet comprising the washed magnetically responsive beads; and combining the droplet comprising the washed magnetically responsive beads with a droplet comprising a reporter molecule having affinity for target analyte captured on the magnetically-responsive beads to yield a second combined droplet; beginning with the second combined droplet, effecting a droplet-based washing protocol to wash the magnetically-responsive beads to yield a second washed droplet comprising the washed magnetically responsive beads; detecting a signal from the second washed droplet which corresponds to the presence, absence and/or quantity of the analyte in the sample.

    Abstract translation: 一种检测样品中的目标分析物的方法，包括执行电润湿介导的液滴操作，从而：将包含对靶分析物具有亲和性的磁响应珠粒的一种或多种免疫测定试剂液滴与潜在地包含靶分析物的一个或多个样品液 以产生第一组合液滴; 从组合的液滴开始，实现基于液滴的洗涤方案以洗涤磁响应珠，以产生包含洗涤的磁响应珠的第一洗涤液滴; 以及将包含洗涤的磁响应珠粒的液滴与包含对在磁响应珠上捕获的靶分析物具有亲和力的报道分子的液滴组合以产生第二组合液滴; 从第二组合液滴开始，实现基于液滴的洗涤方案以洗涤磁响应珠，产生包含洗涤的磁响应珠的第二洗涤液滴; 检测来自第二洗涤液滴的信号，其对应于样品中分析物的存在，不存在和/或数量。

    Capacitance detection in a droplet actuator
    53.
    发明授权
    Capacitance detection in a droplet actuator 有权

    公开(公告)号：US10183292B2

    公开(公告)日：2019-01-22

    申请号：US14968094

    申请日：2015-12-14

    Applicant: Advanced Liquid Logic, Inc.

    Inventor： Ryan A. Sturmer ,  Michael G. Pollack ,  Vamsee K. Pamula ,  Vijay Srinivasan ,  Philip Y. Paik

    IPC: B01L3/00 ,  G01N27/22 ,  G01V3/02

    Abstract: A method, circuit and apparatus for detecting capacitance on a droplet actuator, inter alia, for determining the presence, partial presence or absence of a droplet at an electrode on a droplet actuator by: (a) providing a droplet actuator comprising: (i) a substrate comprising electrodes arranged on the substrate for conducting droplet operations on a surface of the substrate; (ii) a capacitance detection circuit for detecting capacitance at the droplet operations surface at one or more of the electrodes; (b) detecting capacitance at the droplet operations surface at one or more of the electrodes; and (c) determining from the capacitance the presence, partial presence or absence of a droplet at the droplet operations surface at the electrode.

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