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    CONTROLLABLE SELF-ANNEALING MICROGEL PARTICLES FOR BIOMEDICAL APPLICATIONS
    52.
    发明申请
    CONTROLLABLE SELF-ANNEALING MICROGEL PARTICLES FOR BIOMEDICAL APPLICATIONS 审中-公开

    公开(公告)号：US20190151497A1

    公开(公告)日：2019-05-23

    申请号：US16264466

    申请日：2019-01-31

    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

    Inventor： Donald R. Griffin ,  Westbrook Weaver ,  Tatiana Segura ,  Dino Di Carlo ,  Philip Scumpia

    IPC: A61L26/00 ,  A61L27/58 ,  A61L27/22 ,  A61L27/18 ,  A61L27/54 ,  A61L27/56 ,  A61L27/52

    Abstract: A microporous gel system for certain applications, including biomedical applications, includes an aqueous solution containing plurality of microgel particles including a biodegradable crosslinker. In some aspects, the microgel particles act as gel building blocks that anneal to one another to form a covalently-stabilized scaffold of microgel particles having interstitial spaces therein. In certain aspects, annealing of the microgel particles occurs after exposure to an annealing agent that is endogenously present or exogenously added. In some embodiments, annealing of the microgel particles requires the presence of an initiator such as exposure to light. In particular embodiments, the chemical and physical properties of the gel building blocks can be controlled to allow downstream control of the resulting assembled scaffold. In one or more embodiments, cells are able to quickly infiltrate the interstitial spaces of the assembled scaffold.

    LABEL-FREE DIGITAL BRIGHTFIELD ANALYSIS OF NUCLEIC ACID AMPLIFICATION
    53.
    发明申请
    LABEL-FREE DIGITAL BRIGHTFIELD ANALYSIS OF NUCLEIC ACID AMPLIFICATION 审中-公开

    公开(公告)号：US20180373921A1

    公开(公告)日：2018-12-27

    申请号：US16019421

    申请日：2018-06-26

    Applicant: The Regents of the University of California

    Inventor： Dino Di Carlo ,  Aydogan Ozcan ,  Omai B. Garner ,  Hector E. Munoz ,  Carson Riche

    IPC: G06K9/00 ,  C12Q1/6806 ,  G06T7/155 ,  G06F15/18 ,  G06F17/27 ,  C12Q1/6844

    Abstract: An optical readout method for detecting a precipitate (e.g., a precipitate generated from the LAMP reaction) contained within a droplet includes generating a plurality of droplets, at least some which have a precipitate contained therein. The droplets are imaged using a brightfield imaging device. The image is subject to image processing using image processing software executed on a computing device. Image processing isolates individual droplets in the image and performs feature detection within the isolated droplets. Keypoints and information related thereto are extracted from the detected features within the isolated droplets. The keypoints are subject to a clustering operation to generate a plurality of visual “words.” The word frequency obtained for each droplet is input into a trained machine learning droplet classifier, wherein the trained machine learning droplet classifier classifies each droplet as positive for the precipitate or negative for the precipitate.

    CONTROLLABLE SELF-ANNEALING MICROGEL PARTICLES FOR BIOMEDICAL APPLICATIONS
    57.
    发明申请
    CONTROLLABLE SELF-ANNEALING MICROGEL PARTICLES FOR BIOMEDICAL APPLICATIONS 审中-公开
    用于生物医学应用的可控自分离微粒子

    公开(公告)号：US20160279283A1

    公开(公告)日：2016-09-29

    申请号：US15179151

    申请日：2016-06-10

    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

    Inventor： Donald R. Griffin ,  Westbrook Weaver ,  Tatiana Segura ,  Dino Di Carlo ,  Philip Scumpia

    IPC: A61L26/00 ,  A61L27/22 ,  A61L27/52 ,  A61L27/56 ,  A61L27/54

    CPC classification number: A61L26/008 ,  A61L26/0019 ,  A61L26/0047 ,  A61L26/0066 ,  A61L26/0085 ,  A61L26/009 ,  A61L27/18 ,  A61L27/227 ,  A61L27/52 ,  A61L27/54 ,  A61L27/56 ,  A61L27/58 ,  A61L2300/252 ,  A61L2300/412 ,  A61L2400/06 ,  A61L2430/00 ,  A61L2430/34 ,  C08L71/02

    Abstract: A microporous gel system for certain applications, including biomedical applications, includes an aqueous solution containing plurality of microgel particles including a biodegradable crosslinker. In some aspects, the microgel particles act as gel building blocks that anneal to one another to form a covalently-stabilized scaffold of microgel particles having interstitial spaces therein. In certain aspects, annealing of the microgel particles occurs after exposure to an annealing agent that is endogenously present or exogenously added. In some embodiments, annealing of the microgel particles requires the presence of an initiator such as exposure to light. In particular embodiments, the chemical and physical properties of the gel building blocks can be controlled to allow downstream control of the resulting assembled scaffold. In one or more embodiments, cells are able to quickly infiltrate the interstitial spaces of the assembled scaffold.

    Abstract translation: 用于某些应用（包括生物医学应用）的微孔凝胶体系包括含有多个包含可生物降解交联剂的微凝胶颗粒的水溶液。 在一些方面，微凝胶颗粒作为凝胶结构单元，其彼此退火以形成其中具有间隙空间的微凝胶颗粒的共价稳定的支架。 在某些方面，微凝胶颗粒的退火在暴露于内源存在或外源添加的退火剂之后发生。 在一些实施方案中，微凝胶颗粒的退火需要引发剂的存在，例如暴露于光。 在具体实施方案中，可以控制凝胶结构单元的化学和物理性质以允许下游控制所得的组装的支架。 在一个或多个实施方案中，细胞能够快速渗透组装的支架的间隙空间。

    System and method for deforming and analyzing particles
    59.
    发明授权
    System and method for deforming and analyzing particles 有权
    颗粒变形和分析的系统和方法

    公开(公告)号：US09151705B2

    公开(公告)日：2015-10-06

    申请号：US14058028

    申请日：2013-10-18

    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

    Inventor： Dino Di Carlo ,  Daniel R. Gossett ,  Henry T. K. Tse ,  Aram Chung

    IPC: G01N15/14 ,  G01N33/50

    CPC classification number: G01N15/1404 ,  G01N15/1434 ,  G01N15/1436 ,  G01N15/1459 ,  G01N15/147 ,  G01N15/1484 ,  G01N21/64 ,  G01N21/6428 ,  G01N21/645 ,  G01N33/50 ,  G01N33/5091 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1415 ,  G01N2015/1495 ,  G01N2021/6439 ,  G06K9/4604 ,  G06T7/0004 ,  G06T7/0016

    Abstract: A system for deforming and analyzing a plurality of particles carried in a sample volume includes a substrate defining an inlet, configured to receive the sample volume, and an outlet; and a fluidic pathway fluidly coupled to the inlet and the outlet. The fluidic pathway includes a delivery region configured to receive the plurality of particles from the inlet and focus the plurality of particles from a random distribution to a focused state, a deformation region defining an intersection located downstream of the delivery region and coupled to the outlet, and wherein the deformation region is configured to receive the plurality of particles from the delivery region and to transmit each particle in the plurality of particles into the intersection from a single direction, a first branch fluidly coupled to the deformation region and configured to transmit a first flow into the intersection, and a second branch fluidly coupled to the deformation region and configured to transmit a second flow, substantially opposing the first flow, into the intersection, wherein the first flow and the second flow are configured to induce extension of one or more particles in the plurality of particles.

    Abstract translation: 用于变形和分析载体在样品体积中的多个颗粒的系统包括限定入口的衬底，其构造成接收样品体积和出口; 以及流体连接到入口和出口的流体通路。 流体通道包括配置成从入口接收多个颗粒并将多个颗粒从随机分布聚焦到聚焦状态的输送区域，限定位于输送区域下游并且耦合到出口的交叉的变形区域， 并且其中所述变形区域被配置为从所述递送区域接收所述多个颗粒并且将多个颗粒中的每个颗粒从单个方向传播到所述交叉点，所述第一分支流体耦合到所述变形区域并且被配置为传输第一 流入所述十字路口的第二分支和流体耦合到所述变形区域并被配置为将基本上与所述第一流相对的第二流传送到所述交叉点的第二分支，其中所述第一流和所述第二流被配置为诱导一个或多个 多个颗粒中的颗粒。

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