公开(公告)号：US20110224099A1

公开(公告)日：2011-09-15

申请号：US13046531

申请日：2011-03-11

Applicant: David G. Gorenstein ,  Bruce A. Luxon ,  Norbert Herzog ,  Xian Bin Yang

Inventor： David G. Gorenstein ,  Bruce A. Luxon ,  Norbert Herzog ,  Xian Bin Yang

IPC: C40B40/06

CPC classification number: C07H21/00 ,  B01J2219/00378 ,  B01J2219/00387 ,  B01J2219/00497 ,  B01J2219/005 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00592 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00707 ,  B01J2219/00722 ,  C07B2200/11 ,  C12N15/1048 ,  C40B20/04 ,  C40B40/06 ,  C40B40/08 ,  C40B60/14 ,  G01N33/6803 ,  G01N33/6851

Abstract: The present invention includes composition and methods for making and using a combinatorial library having two or more beads, wherein attached to each bead is a unique nucleic acid aptamer that have disposed thereon a unique sequence. The library aptamers may be attached covalently to the one or more beads, which may be polystyrene beads. The aptamers may include phosphorothioate, phosphorodithioate and/or methylphosphonate linkages and may be single or double stranded DNA, RNA or even PNAs.

Abstract translation: 本发明包括用于制备和使用具有两个或多个珠的组合文库的组合物和方法，其中连接到每个珠粒是在其上设置有唯一序列的唯一核酸适体。 文库适体可以共价连接到一个或多个珠，其可以是聚苯乙烯珠。 适体可以包括硫代磷酸酯，二硫代磷酸酯和/或甲基膦酸酯键，并且可以是单链或双链DNA，RNA或甚至PNA。

公开(公告)号：US08013194B2

公开(公告)日：2011-09-06

申请号：US12402372

申请日：2009-03-11

Applicant: Yuon Chiu ,  Stephen A. Cottrell ,  Hsueh Sung Tung ,  Haluk Kopkalli ,  Gustavo Cerri

Inventor： Yuon Chiu ,  Stephen A. Cottrell ,  Hsueh Sung Tung ,  Haluk Kopkalli ,  Gustavo Cerri

IPC: C07C17/00

CPC classification number: C07C17/25 ,  B01J7/00 ,  B01J19/0046 ,  B01J19/14 ,  B01J2219/00452 ,  B01J2219/0059 ,  B01J2219/00594 ,  B01J2219/0072 ,  B01J2219/00759 ,  C07C17/23 ,  C07C17/354 ,  C07C17/38 ,  C07C21/18 ,  C07C19/08

Abstract: A method for producing 1,1,1,2-tetrafluoropropene and/or 1,1,1,2,3-pentafluoropropene using a single set of four unit operations, the unit operations being (1) hydrogenation of a starting material comprising hexafluoropropene and optionally recycled 1,1,1,2,3-pentafluoropropene; (2) separation of the desired intermediate hydrofluoroalkane, such as 1,1,1,2,3,3-hexafluoropropane and/or 1,1,1,2,3-pentafluoropropane; (3) dehydrofluorination of the intermediate hydrofluoroalkane to produce the desired 1,1,1,2-tetrafluoropropene and/or 1,1,1,2,3-pentafluoropropene, followed by another separation to isolate the desired product and, optionally, recycle of the 1,1,1,2,3-pentafluoropropene.

Abstract translation: 使用单组四个单元操作制备1,1,1,2-四氟丙烯和/或1,1,1,2,3-五氟丙烯的方法，单元操作是（1）将包含六氟丙烯的原料氢化 和任选的再循环的1,1,1,2,3-五氟丙烯; （2）所需的中间体氢氟烷烃的分离，例如1,1,1,2,3,3-六氟丙烷和/或1,1,1,2,3-五氟丙烷; （3）中间体氢氟烷烃的脱氟化氢以产生所需的1,1,1,2-四氟丙烯和/或1,1,1,2,3-五氟丙烯，然后进行另一次分离以分离所需产物，并且任选地再循环 的1,1,1,2,3-五氟丙烯。

公开(公告)号：US08008005B2

公开(公告)日：2011-08-30

申请号：US11657209

申请日：2007-01-24

Applicant: Peter J. Belshaw ,  Michael J. Sussman ,  Francesco Cerrina ,  Shane T. Flickinger

Inventor： Peter J. Belshaw ,  Michael J. Sussman ,  Francesco Cerrina ,  Shane T. Flickinger

IPC: C12Q1/68

CPC classification number: C12N15/1031 ,  B01J2219/00274 ,  B01J2219/00439 ,  B01J2219/00441 ,  B01J2219/00454 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00689 ,  B01J2219/007 ,  B01J2219/00722 ,  B82Y30/00 ,  C07B2200/11 ,  C07F9/2408 ,  C07H19/06 ,  C07H19/10 ,  C07H19/16 ,  C07H19/20 ,  C07H21/00 ,  C12N15/10 ,  C40B40/06 ,  C40B50/14 ,  C40B60/14

Abstract: A method is disclosed for the direct synthesis of double stranded DNA molecules of a variety of sizes and with any desired sequence. The DNA molecule to be synthesis is logically broken up into smaller overlapping DNA segments. A maskless microarray synthesizer is used to make a DNA microarray on a substrate in which each element or feature of the array is populated by DNA of a one of the overlapping DNA segments. The DNA segments are released from the substrate and held under conditions favoring hybridization of DNA, under which conditions the segments will spontaneously hybridize together to form the desired DNA construct. This method makes possible the remote assembly of DNA sequence, through a process analogous to facsimile transmission of documents, since the information on DNA to be made can be transmitted remotely to an instrument which can then synthesize any needed DNA sequence from the information.

Abstract translation: 公开了用于直接合成各种尺寸和任何所需顺序的双链DNA分子的方法。 要合成的DNA分子在逻辑上分解成更小的重叠DNA片段。 无掩模微阵列合成器用于在基底上制备DNA微阵列，其中阵列的每个元件或特征由重叠DNA片段之一的DNA填充。 DNA片段从底物释放并保持在有利于DNA杂交的条件下，在该条件下，片段将自发地杂交在一起形成所需的DNA构建体。 该方法可以通过类似于文件传真传输的过程来远程组装DNA序列，因为要制备的DNA的信息可以远程传输到仪器，然后可以从信息中合成任何所需的DNA序列。

公开(公告)号：US20110184135A1

公开(公告)日：2011-07-28

申请号：US13081653

申请日：2011-04-07

Applicant: Daryoosh Beigzadeh ,  Richard E. Campbell, JR. ,  David D. Devore ,  Duane R. Romer ,  James C. Stevens ,  Francis J. Timmers ,  Hendrik E. Tuinstra ,  Paul C. Vosejpka

Inventor： Daryoosh Beigzadeh ,  Richard E. Campbell, JR. ,  David D. Devore ,  Duane R. Romer ,  James C. Stevens ,  Francis J. Timmers ,  Hendrik E. Tuinstra ,  Paul C. Vosejpka

IPC: C08F4/6592 ,  B01J31/14 ,  C08F4/52 ,  C08F4/642

CPC classification number: C08F10/02 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00702 ,  B01J2219/00736 ,  B01J2219/00738 ,  C08F4/659 ,  C08F4/65908 ,  C08F10/00 ,  C08F2410/01 ,  Y10S526/943 ,  C08F4/65912 ,  C08F4/6592 ,  C08F210/02 ,  C08F212/08 ,  C08F210/16 ,  C08F210/14 ,  C08F210/06

Abstract: A composition comprising a polymerization modifier for the copolymerization of at least one olefin monomer and 1-octene and a polymerization process using the polymerization modifier.

Abstract translation: 包含用于共聚至少一种烯烃单体和1-辛烯的聚合改性剂和使用聚合改性剂的聚合方法的组合物。

公开(公告)号：US20110136696A1

公开(公告)日：2011-06-09

申请号：US13029071

申请日：2011-02-16

Applicant: David L. Heiner ,  Aaron C. Jones ,  Steven P. Fambro ,  Mark J. Nibbe ,  Steve R. Burgett ,  Brett M. Ellman ,  Michal Lebl

Inventor： David L. Heiner ,  Aaron C. Jones ,  Steven P. Fambro ,  Mark J. Nibbe ,  Steve R. Burgett ,  Brett M. Ellman ,  Michal Lebl

IPC: C40B50/00

CPC classification number: B82Y30/00 ,  B01J19/0046 ,  B01J2219/00283 ,  B01J2219/00315 ,  B01J2219/00326 ,  B01J2219/0036 ,  B01J2219/00423 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00612 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00693 ,  B01J2219/00722 ,  B01J2219/00725 ,  C40B60/14 ,  Y10T436/115831 ,  Y10T436/12

Abstract: Described is a system and method for synthesizing polymeric molecules such as oligonucleotides and polypeptides. The system is capable of continuously synthesizing molecules by providing an array of reaction sites and an array of stations for carrying out synthetic manipulations. The reaction sites in the former array can be placed in a fixed order and at fixed intervals relative to each other. Similarly, the stations can be placed in a fixed order and at fixed intervals relative to each other. The two arrays can be moved relative to each other such that the stations carry out desired steps of a reaction scheme at each reaction site. The relative locations of the stations and the schedule for the relative movement can correlate with the order and duration of reaction steps in the reaction scheme such that once a reaction site has completed a cycle of interacting with the full array of stations then the reaction scheme is complete.

Abstract translation: 描述了用于合成聚合物分子如寡核苷酸和多肽的系统和方法。 该系统能够通过提供反应位点阵列和用于进行合成操作的站阵列来连续合成分子。 前一阵列中的反应位点可以相对于彼此以固定的顺序和固定的间隔放置。 类似地，站可以以固定的顺序相对于彼此以固定的间隔放置。 两个阵列可以相对于彼此移动，使得站在每个反应位置进行反应方案的期望步骤。 站的相对位置和相对运动的时间表可以与反应方案中的反应步骤的顺序和持续时间相关联，使得一旦反应位点完成了与整个站阵列相互作用的循环，则反应方案为 完成。

公开(公告)号：US07893233B2

公开(公告)日：2011-02-22

申请号：US12725230

申请日：2010-03-16

Applicant: Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

Inventor： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

IPC: C07H21/04 ,  C12N15/11

CPC classification number: C12Q1/6827 ,  B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00536 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00729 ,  B82Y30/00 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6876 ,  C12Q2600/156 ,  C40B40/06 ,  C40B60/14 ,  Y02A50/58 ,  C12Q2565/501 ,  C12Q2561/125 ,  C12Q2537/143 ,  C12Q2565/514

Abstract: The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support.

Abstract translation: 本发明描述了用于鉴定多个靶核苷酸序列中一个或多个单碱基变化，插入，缺失或易位不同的多个序列中的一个或多个的方法。 该方法包括连接阶段，捕获阶段和检测阶段。 连接阶段利用具有目标序列特异性部分的一个寡核苷酸探针与可寻址阵列特异性部分之间的连接检测反应和具有靶序列特异性部分和可检测标记的第二寡核苷酸探针。 在连接阶段之后，捕获阶段通过将连接的寡核苷酸探针与具有固定化捕获寡核苷酸阵列的固体支持物杂交进行，其中至少一些与可寻址阵列特异性部分互补。 捕获阶段完成后，进行检测阶段以检测与固体支持物杂交的连接的寡核苷酸探针的标记。

公开(公告)号：US20110020876A1

公开(公告)日：2011-01-27

申请号：US10639186

申请日：2003-08-11

Applicant: Peter Wilding ,  Larry J. Kricka

Inventor： Peter Wilding ,  Larry J. Kricka

IPC: C12P19/34 ,  C12M1/00

CPC classification number: B01D61/18 ,  B01D67/0058 ,  B01D67/0072 ,  B01D71/02 ,  B01F5/0601 ,  B01J19/0046 ,  B01J19/0093 ,  B01J2219/0059 ,  B01J2219/00722 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502753 ,  B01L7/52 ,  B01L7/525 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/10 ,  B01L2200/146 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/1827 ,  B01L2300/1861 ,  B01L2400/0406 ,  B01L2400/0487 ,  C12M41/46 ,  C12Q1/6844 ,  C12Q1/686 ,  C40B40/06 ,  Y10S366/03 ,  Y10S435/808 ,  Y10S435/81 ,  Y10S435/814 ,  Y10T436/25125

Abstract: Disclosed are devices for amplifying a preselected polynucleotide in a sample by conducting a polynucleotide amplification reaction. The devices are provided with a substrate microfabricated to include a polynucleotide amplification reaction, chamber, having at least one cross-sectional dimension of about 0.1 to 1000 μm. The device also includes at least one port in fluid communication with the reaction chamber, for introducing a sample to the chamber, for venting the chamber when necessary, and, optionally, for removing products or waste material from the device. The reaction chamber may be provided with reagents required for amplification of a preselected polynucleotide. The device also may include means for thermally regulating the contents of the reaction chamber, to amplify a preselected polynucleotide. Preferably, the reaction chamber is fabricated with a high surface to volume ratio, to facilitate thermal regulation.

Abstract translation: 公开了通过进行多核苷酸扩增反应来扩增样品中预选择的多核苷酸的装置。 所述装置设置有微加工的底物，以包括具有约0.1至1000μm的至少一个横截面尺寸的多核苷酸扩增反应室。 该装置还包括与反应室流体连通的至少一个端口，用于将样品引入腔室，以便在需要时排出腔室，并且可选地用于从装置中移出产品或废料。 反应室可以具有扩增预选择的多核苷酸所需的试剂。 该装置还可以包括用于热调节反应室的内容物的装置，以扩增预先选择的多核苷酸。 优选地，以高的表面与体积比制造反应室，以便于热调节。

公开(公告)号：US20100324266A1

公开(公告)日：2010-12-23

申请号：US12797559

申请日：2010-06-09

Applicant: Anthony D. Barone ,  Glenn H. McGall

Inventor： Anthony D. Barone ,  Glenn H. McGall

IPC: C07K2/00 ,  C07D209/08 ,  C07C69/96 ,  C07D317/62 ,  C07D403/12 ,  C07D405/12 ,  C07D311/54 ,  C07D311/20 ,  C07D215/22 ,  C07F7/10 ,  C07H19/10 ,  C07K14/00 ,  C07K14/575 ,  C07K17/14

CPC classification number: B82Y30/00 ,  B01J2219/00432 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00731 ,  C07B2200/11 ,  C07H19/06 ,  C07H19/10 ,  C07H19/16 ,  C07H19/20 ,  C07H21/00 ,  C07K1/04 ,  C07K1/047 ,  C07K1/062 ,  C07K1/063 ,  C07K1/064 ,  C07K1/065 ,  C07K1/066 ,  C40B40/06 ,  C40B40/10 ,  C40B40/12 ,  C40B60/14 ,  Y02P20/55

Abstract: Novel compounds are provided, which are useful as linking groups in chemical synthesis, preferably in the solid phase synthesis of oligonucleotides and polypeptides. These compounds are generally photolabile and comprise protecting groups which can be removed by photolysis to unmask a reactive group. The protecting group has the general formula Y, wherein Y is a chemical structure as shown in FIG. 1. Also provided is a method of forming, from component molecules, a plurality of compounds on a support, each compound occupying a separate predefined region of the support, using the protected compounds described above.

Abstract translation: 提供新的化合物，其可用作化学合成中的连接基团，优选在寡核苷酸和多肽的固相合成中。 这些化合物通常是光不稳定的并且包括可以通过光解去除以揭示反应性基团的保护基团。 保护基具有通式Y，其中Y是如图1所示的化学结构。 还提供了使用上述受保护的化合物从成分分子形成多个化合物在载体上的方法，每种化合物占据支持体的单独的预定区域。

公开(公告)号：US07838466B2

公开(公告)日：2010-11-23

申请号：US10157442

申请日：2002-05-29

Applicant: Xiaolian Gao ,  Xiaochuan Zhou ,  Erdogan Gulari

Inventor： Xiaolian Gao ,  Xiaochuan Zhou ,  Erdogan Gulari

IPC: C40B50/00

CPC classification number: C07K1/047 ,  B01J19/0046 ,  B01J2219/00434 ,  B01J2219/00439 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00689 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00731 ,  B82Y30/00 ,  C07B2200/11 ,  C07H21/00 ,  C07K1/045 ,  C40B40/06 ,  C40B40/10 ,  C40B40/12 ,  C40B50/14 ,  C40B60/14 ,  G03F7/00

Abstract: This invention provides method and apparatus for performing chemical and biochemical reactions in solution using in situ generated photo-products as reagent or co-reagent. In particular, the present invention provides methods and devices for selectively converting photogenerated reagent precursors to photogenerated reagents comprising a substrate comprising at least one solid surface containing a plurality of isolated reaction sites; and an optical system operably linked to the substrate comprising a light source and a computer-controlled spatial optical modulator to form an irradiation pattern, wherein the irradiation pattern selectively irradiates a plurality of reaction sites without use of a photolithographic mask. The method and apparatus of the present invention have applications in parallel synthesis of molecular sequence arrays on solid surfaces.

Abstract translation: 本发明提供了使用原位产生的光产物作为试剂或共试剂在溶液中进行化学和生物化学反应的方法和装置。 特别地，本发明提供了用于选择性地将光生试剂前体转化成光生试剂的方法和装置，所述光生试剂包含含有至少一个含有多个分离的反应位点的固体表面的底物; 以及可操作地连接到所述基板的光学系统，所述光学系统包括光源和计算机控制的空间光学调制器以形成照射图案，其中所述照射图案选择性地照射多个反应部位而不使用光刻掩模。 本发明的方法和装置具有在固体表面上并行合成分子序列阵列的应用。

公开(公告)号：US07820951B2

公开(公告)日：2010-10-26

申请号：US11852480

申请日：2007-09-10

Applicant: Edward Earl King ,  James Edward Thomas

Inventor： Edward Earl King ,  James Edward Thomas

IPC: H05B6/68

CPC classification number: H05B6/806 ,  B01J3/03 ,  B01J19/126 ,  B01J2219/0027 ,  B01J2219/00283 ,  B01J2219/00335 ,  B01J2219/00495 ,  B01J2219/0059 ,  B01J2219/1233 ,  B01J2219/1254 ,  B01J2219/1263 ,  B01J2219/1284 ,  H05B6/705 ,  H05B6/708 ,  Y10T436/25

Abstract: An instrument for carrying out controlled microwave assisted chemical processes, and that is particularly useful for handling relatively small samples. The instrument includes a microwave-transparent reaction vessel with an open mouth, a pressure-resistant seal on the mouth of the vessel, and a needle, portions of which penetrate the seal with a first end of the needle and provide fluid communication into the vessel. A pressure transducer is at the opposite end of the needle and in fluid communication with the interior of the vessel through the needle. The instrument defines a pressure control flow path from a portion of the needle outside of the vessel to a fluid port, the flow path being in communication with the needle, the interior of the vessel and the transducer. A controllable pressure release valve for the flow path is associated with the port.