公开(公告)号：US20230307095A1

公开(公告)日：2023-09-28

申请号：US18191477

申请日：2023-03-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Christian KOLLER ,  Marcin SIKORA ,  Peter VANDER HORN

IPC: G16B40/10 ,  G16B5/00 ,  G16B20/00 ,  G16B25/00 ,  G16B30/00

CPC classification number: G16B40/10 ,  G16B5/00 ,  G16B20/00 ,  G16B25/00 ,  G16B30/00 ,  C12Q1/6869

Abstract: A method for nucleic acid sequencing includes receiving nucleic acid sequencing data from a sequencing instrument that receives and processes a sample nucleic acid in a sequencing-by-synthesis process. The method also includes generating a set of candidate sequences of bases for the observed or measured nucleic acid sequencing data by determining a predicted signal for candidate sequences using a simulation framework. The simulation framework incorporates an estimated carry forward rate (CFR), an estimated incomplete extension rate (IER), an estimated droop rate (DR), an estimated reactivated molecules rate (RMR), and an estimated termination failure rate (TFR), the RMR being greater than or equal to zero and the TFR being lesser than one. The method also includes identifying, from the set of candidate sequences of bases, a candidate sequence as corresponding to the sequence for the sample nucleic acid.

公开(公告)号：US11768156B2

公开(公告)日：2023-09-26

申请号：US17936947

申请日：2022-09-30

Applicant: Life Technologies Corporation

Inventor： Kyle Gee ,  Kathleen Kihn ,  Yi-Zhen Hu ,  Yexin Wu ,  Kathleen Free

IPC: G01N21/64 ,  C07D311/82 ,  C07D209/10 ,  C07F15/00 ,  C07F9/24

CPC classification number: G01N21/6486 ,  C07D209/10 ,  C07D311/82 ,  C07F9/2466 ,  C07F15/0093 ,  G01N21/6428

Abstract: Provided herein are compounds, compositions, kits, uses, and methods for assessing the viability of cells and/or quantifying the amount of live and dead cells in a mixture using differential stains. In some embodiments, the compounds are used in culture media or can function independently of fixation and/or permeabilization. In some embodiments, the compounds comprise a platinum atom. In some embodiments, the compounds comprise a nitrogen mustard moiety.

公开(公告)号：US11739316B2

公开(公告)日：2023-08-29

申请号：US16908567

申请日：2020-06-22

Applicant: THERMO FISHER SCIENTIFIC BALTICS UAB ,  LIFE TECHNOLOGIES CORPORATION

Inventor： Arvydas Lubys ,  Inga Cikotiene ,  Zana Kapustina ,  Arturas Berezniakovas ,  Justina Medziune ,  Simona Zeimyte ,  Mark Andersen ,  Michael Allen ,  Sihong Chen

IPC: C12N15/10 ,  C12N15/115 ,  C12Q1/6806

CPC classification number: C12N15/1065 ,  C12N15/115 ,  C12Q1/6806 ,  C12N2310/3517

Abstract: The present disclosure describes oligonucleotide-tethered nucleotides, methods of making them, and methods of using them. The oligonucleotide-tethered nucleotides comprise, in some embodiments, a nucleotide linked to an oligonucleotide of from about 3 to about 100 nucleotides in length. These oligonucleotide-tethered nucleotides can be used to label a plurality of different types of nucleic acids in a plurality of different situations with a known oligonucleotide, which can serve as a barcode in some embodiments. The resulting oligonucleotide-labeled nucleic acids oligonucleotides can be used in a variety of nucleic acid sequencing methods.

公开(公告)号：US20230221280A1

公开(公告)日：2023-07-13

申请号：US18084587

申请日：2022-12-20

Applicant: Life Technologies Holdings PTE Limited ,  Life Technologies Corporation

Inventor： Beng Heng LIM ,  Wuh Ken LOH ,  Hwee Siong KUAH ,  Jing Han WONG ,  Jefferson Cruz GANGCUANGCO ,  Jin Xin ONG ,  Soo Yong LAU ,  Yanping XU ,  Victor SHAPIRO ,  Zhi Da TEH ,  Chee Woei CHONG ,  Kuan Moon BOO

IPC: G01N27/447

CPC classification number: G01N27/44721

Abstract: A gel electrophoresis system includes a base module and a camera module. The base module includes a cassette slot for receiving a gel electrophoresis cassette, and a light element that functions to illuminate the gel electrophoresis cassette. The camera module is selectively attachable to and detachable from the base module. When attached to the base module, the camera module facilitates imaging of the gel electrophoresis cassette and provides additional imaging capabilities.

公开(公告)号：US20230204537A1

公开(公告)日：2023-06-29

申请号：US18056194

申请日：2022-11-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Keith G. Fife ,  Jungwook Yang

IPC: G01N27/414 ,  G06F30/392 ,  G06F30/394

CPC classification number: G01N27/4145 ,  G06F30/392 ,  G06F30/394 ,  G01N27/4148

Abstract: A high data rate integrated circuit, such as an integrated circuit including a large sensor array, may be implemented using clock multipliers in individual power domains, coupled to sets of transmitters, including a transmitter pair configuration. Reference clock distribution circuitry on the integrated circuit distributes a relatively low speed reference clock. In a transmitter pair configuration, each pair comprises a first transmitter and a second transmitter in a transmitter power domain. Also, each pair of transmitters includes a clock multiplier connected to the reference clock distribution circuitry, and disposed between the first and second transmitters, which produces a local transmit clock.

公开(公告)号：US20230193379A1

公开(公告)日：2023-06-22

申请号：US17811192

申请日：2022-07-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Vadim Mozhayskiy ,  Yutao FU ,  Earl HUBBELL

IPC: C12Q1/6874 ,  C12Q1/6825 ,  C12N15/10

CPC classification number: C12Q1/6874 ,  C12Q1/6825 ,  C12N15/1058

Abstract: A method for preparing a homopolymer recalibration panel includes: extracting, from a set of amplicons used in sequencing-by-synthesis, a set of candidate amplicons satisfying a first set of criteria, wherein the first set of criteria includes amplicons known to belong to high-confidence regions of a reference genome with no variants; and selecting, from the set of candidate amplicons, a reduced set of amplicons satisfying a second set of criteria, wherein the second set of criteria includes amplicons that together comprise at least a minimal threshold number of homopolymers of each homopolymer length between a predetermined minimal homopolymer length and a predetermined maximal homopolymer length for one or more of homopolymer types A, T, C, and G.

公开(公告)号：US11680905B2

公开(公告)日：2023-06-20

申请号：US17572926

申请日：2022-01-11

Applicant: LIFE TECHNOLOGIES HOLDINGS PTE LIMITED ,  LIFE TECHNOLOGIES CORPORATION

Inventor： Ming Song Chen ,  Chee Woei Chong ,  Wuh Ken Loh ,  Wern Yuh Foo ,  Kuan Moon Boo ,  Norbert Leclerc ,  Kristina Uhlendorf ,  Reiner Koellner ,  Torsten Fuchs ,  Stefan Breitfelder

IPC: G01N21/64

CPC classification number: G01N21/6452 ,  G01N21/6428 ,  G01N21/6458 ,  G01N2021/6478

Abstract: A biological analysis system can include an excitation module and an emission module. The excitation module can include a collimator element for receiving excitation light from the excitation light source and transmitting collimated excitation light in a first direction, and a plurality of excitation mirrors arrayed along the excitation light path, each excitation mirror disposed at an acute angle relative to the first direction and configured to reflect collimated excitation light along a second direction. The emission module can be positioned to receive excitation light transmitted along the second direction and can include a sample block comprising a plurality of sample receptacles positioned to receive a beam of collimated excitation light, and a plurality of photodetectors configured to receive emission light transmitted from a respective sample receptacle in a direction transverse to the second direction of the excitation light path.

公开(公告)号：US20230175046A1

公开(公告)日：2023-06-08

申请号：US18104599

申请日：2023-02-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David LIGHT ,  Wolfgang HINZ ,  Ronald CICERO ,  Christina INMAN ,  Paul KENNEY ,  Alexander MASTROIANNI ,  Roman ROZHKOV ,  Yufang WANG ,  Jeremy GRAY ,  Marc GLAZER ,  Dmitriy GREMYACHINSKIY

IPC: C12Q1/6837 ,  C12Q1/6869 ,  C12Q1/6834 ,  C12Q1/6806

CPC classification number: C12Q1/6837 ,  C12Q1/6869 ,  C12Q1/6834 ,  C12Q1/6806

Abstract: A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.

公开(公告)号：US20230167210A1

公开(公告)日：2023-06-01

申请号：US18101785

申请日：2023-01-26

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir FONNUM ,  Grete Irene MODAHL ,  Nini Hofsloekken KJUS ,  Astrid Evenroed MOLTEBERG ,  Diem Thuy Thi TRAN ,  Jo AASERUD ,  M. Talha GOKMEN ,  Steven M. MENCHEN ,  Carl FULLER ,  Luisa ANDRUZZI ,  Wolfgang HINZ

IPC: C08F130/08 ,  C07F7/18 ,  C08F8/12 ,  C12Q1/686 ,  C08F2/20 ,  C08F2/26 ,  C08F230/08 ,  C08F6/24 ,  C08F222/38 ,  C08F8/32 ,  C08F8/34 ,  C12P19/34 ,  C12Q1/6874 ,  G01N27/414 ,  C08J9/20

CPC classification number: C08F130/08 ,  C07F7/1804 ,  C08F8/12 ,  C12Q1/686 ,  C07F7/188 ,  C08F2/20 ,  C08F2/26 ,  C08F230/085 ,  C08F6/24 ,  C08F222/385 ,  C08F8/32 ,  C08F8/34 ,  C12P19/34 ,  C12Q1/6874 ,  G01N27/4145 ,  C08J9/20 ,  Y10T428/2982 ,  C08F2800/20 ,  C08F220/603

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

公开(公告)号：US11654408B2

公开(公告)日：2023-05-23

申请号：US16421168

申请日：2019-05-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Trevor G. Williams

IPC: B01F27/2111 ,  B01F35/513 ,  B01F35/41 ,  C12M1/02 ,  C12M1/00 ,  C12M1/10 ,  B01F27/07 ,  B01F27/2121 ,  B01F101/44

CPC classification number: B01F35/4111 ,  B01F27/071 ,  B01F27/2111 ,  B01F27/2121 ,  B01F35/513 ,  C12M1/007 ,  C12M1/02 ,  C12M1/10 ,  B01F2101/44

Abstract: A method for operating a bioreactor includes passing a drive shaft into a first tubular connector and a second tubular connector projecting from the first tubular connector, the first tubular connector and the second tubular connector being at least partially disposed within a container, the first tubular connector being more flexible than the second tubular connector, the second tubular connector having a length that comprises at least 20% of a combined length of the first tubular connector and the second tubular connector. Rotating the drive shaft so as to rotate the first tubular connector and the second tubular connector within the container.