公开(公告)号：US20180172685A1

公开(公告)日：2018-06-21

申请号：US15498965

申请日：2017-04-27

Applicant: Fenwal, Inc.

Inventor： Christopher J. Wegener ,  Kyungyoon Min

IPC: G01N33/569 ,  C12M1/00 ,  C12M1/36 ,  G01N33/543

CPC classification number: G01N33/56972 ,  A61M1/0281 ,  A61M1/265 ,  A61M1/3496 ,  A61M1/3618 ,  A61M1/362 ,  C12M29/04 ,  C12M29/14 ,  C12M41/48 ,  C12M47/04 ,  G01N33/54326 ,  G01N33/54366

Abstract: A cell processing system includes a first processor connectable to a source container filled with a biological fluid, a second processor, and a controller coupled to the processors. The first processor includes a separator configured to separate the biological fluid into at least two streams of material, and a first container configured to receive one of the streams. The second processor includes a magnetic separator configured to select target cells, the target cells being associated with magnetic particles, a second, pass-through container associated with the magnetic separator, the second container connected at a first end to the first container, and a third container connected to a second end of the pass-through container. One of the processors includes at least one pump configured to transfer material between the separator and the first container, and between the first container and the second container.

公开(公告)号：US20180149655A1

公开(公告)日：2018-05-31

申请号：US15807454

申请日：2017-11-08

Applicant: ELECTRONICS AND TELECOMMUNICATIONS RESEARCH INSTITUTE

Inventor： Hyo-Bong HONG ,  Seung-Min CHOI ,  Jae-Chan JEONG

IPC: G01N33/574 ,  G01N1/40 ,  G01N33/68 ,  G01N33/543

CPC classification number: G01N33/57484 ,  B01J2219/00299 ,  B01L3/505 ,  G01N1/405 ,  G01N1/4077 ,  G01N33/543 ,  G01N33/54326 ,  G01N33/54346 ,  G01N33/6893 ,  G01N2800/26

Abstract: This invention relates to a kit for detecting a target material and a method of detecting a target material using the same.

公开(公告)号：US09964469B2

公开(公告)日：2018-05-08

申请号：US14679216

申请日：2015-04-06

Applicant: Senior Scientific LLC

Inventor： Edward R Flynn ,  Erika Vreeland

IPC: G01N1/04 ,  B03C1/28 ,  G01N21/59 ,  G01N33/543

CPC classification number: A61B10/0233 ,  A61B90/39 ,  A61B2017/00876 ,  A61B2090/3908 ,  A61B2090/3954 ,  B03C1/28 ,  B03C2201/18 ,  G01N1/04 ,  G01N21/59 ,  G01N33/54326 ,  G01N2201/02 ,  G01N2201/061

Abstract: Apparatuses and methods for removing magnetic particles from suspensions are described. One embodiment of the apparatus is called a magnetic needle.

公开(公告)号：US20180113126A1

公开(公告)日：2018-04-26

申请号：US15793445

申请日：2017-10-25

Applicant: Fannin Innovation Studio, Inc.

Inventor： Dev Chatterjee ,  Leo LlNBECK, III ,  Michael John Heffernan ,  Atul Varadhachary

IPC: G01N33/543 ,  G01N33/487 ,  G01N35/00 ,  B01L3/00 ,  H01F7/06 ,  H01F7/121

CPC classification number: G01N33/54326 ,  B01L3/502715 ,  B01L3/50855 ,  G01N33/48785 ,  G01N33/54386 ,  G01N35/0098 ,  G01N2035/00574 ,  H01F7/064 ,  H01F7/121

Abstract: The invention herein relates to conducting assays with an apparatus including a substantially transparent assay cartridge loaded with magnetic beads, and a magnet carrier base positioned below a scanning platform holding the assay cartridge. The assay cartridge includes magnetic beads, sample and control solutions in some wells, and assay reagents in others. A microcomputer controls a stepping motor which controls movement of the magnet carrier base, and causes the magnetic beads to travel from one well to another. An electromagnetic coil-spring assembly induces mixing of well contents with the magnetic beads on actuation. The assay cartridge is authenticated by sending its encoded identifier to a server or website, and assay instructions are provided remotely to the microcomputer. Following assay completion, the cartridge can have color change or other assay indication detected, and the results sent to the server or website or another recipient.

公开(公告)号：US20180074049A1

公开(公告)日：2018-03-15

申请号：US15819221

申请日：2017-11-21

Applicant: ALBERT EINSTEIN COLLEGE OF MEDICINE, INC.

Inventor： Johnny C. LOKE ,  Harry OSTRER

IPC: G01N33/543 ,  G01N33/68

CPC classification number: G01N33/54326 ,  G01N33/6845

Abstract: Methods and compositions are disclosed for rapid functional analysis of gene variants based on analysis of protein-protein and protein-nucleic acid interactions.

公开(公告)号：US09908117B2

公开(公告)日：2018-03-06

申请号：US14861677

申请日：2015-09-22

Applicant: INDUSTRY-ACADEMIC COOPERATION FOUNDATION YONSEI UNIVERSITY

Inventor： Hyo Il Jung ,  Kyung-A Hyun ,  Tae Yoon Lee

IPC: B01L3/00 ,  G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  G01N33/543 ,  G01N33/50 ,  G01N33/569 ,  B01F5/06 ,  B01F13/00

CPC classification number: B01L3/502761 ,  B01F5/0647 ,  B01F5/0655 ,  B01F13/0059 ,  B01L3/502707 ,  B01L3/5085 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/044 ,  B01L2300/0858 ,  B01L2300/0883 ,  B01L2400/0409 ,  B01L2400/043 ,  G01N33/5002 ,  G01N33/54326 ,  G01N33/5434 ,  G01N33/54346 ,  G01N33/56972 ,  G01N2333/70589

Abstract: The present invention relates to a microfluidic separation device, a separation method using the same and a kit for separating circulating rare cells from blood using the same, and more particularly, to a microfluidic-based separation technology for fixing target particles of a sample, which have a specific affinity for magnetic nanoparticles, to a device by use of a magnetic material, and for isolating the sample from which the target particles have been removed. The present invention may be effectively applied to remove leukocytes from a blood sample in order to isolate circulating rare cells (CRCs), particularly circulating tumor cells (CTCs).

公开(公告)号：US09902987B2

公开(公告)日：2018-02-27

申请号：US14371488

申请日：2013-01-09

Applicant: BIOMERIEUX

Inventor： Jean-Claude Raymond ,  David Mosticone ,  Antoine Vimont ,  Florent Baril ,  Jean-Pierre Flandrois ,  Thomas Junillon ,  Benoit Mallen

IPC: C12Q1/00 ,  C12Q1/10 ,  C12C1/00 ,  C12Q1/04 ,  G01N33/569 ,  G01N33/543 ,  B01L3/00 ,  C12M1/34

CPC classification number: C12Q1/10 ,  B01L3/5029 ,  C12C1/00 ,  C12M41/36 ,  C12Q1/00 ,  C12Q1/04 ,  G01N33/54326 ,  G01N33/569 ,  G01N2333/255

Abstract: A method for detecting at least one microorganism present in a sample, that includes: a) in a first container, bringing the sample into contact with at least one culture medium, b) placing the first container in suitable conditions to permit growth of the microorganism or microorganisms, c) bringing some or all of the mixture being made of the sample and the culture medium into contact with a reaction mixture and a substrate for capturing the microorganism(s) in the first container or in a second container, the reaction mixture having a device for detecting the microorganism(s); d) detecting, within the first or second container, the presence of the microorganism or microorganisms detected by the detecting device and fixed on the capture substrate.

公开(公告)号：US20180052155A1

公开(公告)日：2018-02-22

申请号：US15238315

申请日：2016-08-16

Applicant: Nathaniel MARTINEZ ,  Andres MARTINEZ

Inventor： Nathaniel MARTINEZ ,  Andres MARTINEZ

IPC: G01N33/543

CPC classification number: G01N33/54333 ,  C12Y304/21004 ,  C12Y304/21009 ,  G01N33/54306 ,  G01N33/54326 ,  G01N2333/96441 ,  G01N2333/976

Abstract: An assay device including a loading zone for a sample that may contain an analyte, an activator in communication with the loading zone from which at least one activator is displaced by presence of the analyte, an amplifier in an inactive state in communication with the activator that becomes activated in the presence of the activator, a biomatrix barrier in communication with the activated amplifier that is degraded or modified by the activated amplifier, and an indicator responsive to the degradation or modification of the biomatrix barrier, which in turn reflects a concentration of the analyte in the sample loaded on the device. The selection of analyte or displacement of the activator by presence of the analyte may occur off-platform and/or may rely on use of non-covalent interactions. The activator may include an enzyme or other reagent that activates the amplifier. Also, associated methods.

公开(公告)号：US20180045712A1

公开(公告)日：2018-02-15

申请号：US15729957

申请日：2017-10-11

Applicant: Menarini Silicon Biosystems, Inc.

Inventor： Galla Chandra RAO ,  Brad FOULK ,  Denis SMIRNOV ,  Karl NIELSEN

IPC: G01N33/50 ,  G01N33/543 ,  G01N33/574 ,  G01N33/68 ,  G01N33/58

CPC classification number: G01N33/5005 ,  G01N33/5044 ,  G01N33/54313 ,  G01N33/54326 ,  G01N33/54333 ,  G01N33/57492 ,  G01N33/582 ,  G01N33/583 ,  G01N33/585 ,  G01N33/6854

Abstract: The disclosed invention includes methods and kits for the removal of white blood cells from samples of immunomagnetically enriched rare cells by treating the sample with a leukocyte marker conjugated to a hapten which adheres to the white blood cells and treating the resulting product with a second medium that adheres to the hapten of the white blood cells that are labeled with the leukocyte marker conjugated to hapten and removing the labeled white blood cells.

公开(公告)号：US09891227B2

公开(公告)日：2018-02-13

申请号：US14701267

申请日：2015-04-30

Applicant: Academia Sinica

Inventor： Konan Peck ,  Pan-Chyr Yang ,  Yi-Chung Chang

IPC: G01N33/53 ,  G01N33/58 ,  G01N33/543 ,  G01N33/569

CPC classification number: G01N33/585 ,  G01N33/5308 ,  G01N33/54326 ,  G01N33/54333 ,  G01N33/56938

Abstract: Methods of assaying a biological target are disclosed. The method comprises: (a) providing a sample containing the biological target; (b) providing biotin-labeled first aptamers conjugated to a gold nanoparticle (GNP), and second aptamers conjugated to a magnetic bead, wherein the first and the second aptamers exhibit specific binding affinities to the target; (c) incubating the sample with the first and the second aptamers to obtain target-bound aptamers; (d) separating the target-bound aptamers from unbound aptamers; (e) eluting the first aptamers from the GNP; (f) incubating the eluted biotin-labeled first aptamers with streptavidin-magnetic beads and reporter gold nanoparticles (GNPs) to obtain a complex comprising: the bead, the first aptamers, attached to the bead; and the reporter GNPs captured by the bead through the first aptamers; (g) eluting the reporter GNPs captured; and (h) detecting the target by measuring and analyzing a light-scattering signal of the eluted reporter GNPs.