公开(公告)号：US20250101392A1

公开(公告)日：2025-03-27

申请号：US18662446

申请日：2024-05-13

Applicant: Genomatica, Inc.

Inventor： Andrew M. SHUMAKER ,  Bernardo M.T. DA COSTA ,  Kevin HOLDEN ,  Louis G. HOM ,  Tarah S. BARON ,  Noah HELMAN

IPC: C12N9/18 ,  C12N9/10 ,  C12P7/6436

Abstract: The disclosure relates to enzyme variants with improved ester synthase properties for the production of fatty acid esters. Further contemplated are recombinant host cells that express such variants, cell cultures comprising the recombinant host cells and fatty acid ester compositions produced by such recombinant host cells.

公开(公告)号：US12234463B2

公开(公告)日：2025-02-25

申请号：US17255352

申请日：2019-06-26

Applicant: Genomatica, Inc.

Inventor： Harish Nagarajan ,  Tae Hoon Yang ,  Ali Khodayari

IPC: C12N9/16 ,  C12N9/02 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12P5/00 ,  C12P5/02 ,  C12P7/16 ,  C12P7/18 ,  C12P7/24 ,  C12P7/42 ,  C12P7/46 ,  C12P7/6409 ,  C12P13/00

Abstract: Described herein are engineered cells including ones having synthetic methylotrophy which include an NADH-dependent enzyme capable of converting G3P to 3PG (e.g., B. methanolicus gapN) and/or fructose-1,6-bisphosphatase, along with hexulose-6-phosphate synthase, 6-phospho-3-hexuloisomerase, a phosphoketolase, or a combination thereof. Engineered cells of the disclosure beneficially maintain adequate pool sizes of phosphorylated C3 and/or C4 compounds, and/or provide increased levels of NADPH. As such, the modifications allow for the generation of C6 compounds from C1 (e.g., a methanol feedstod) and C5 compounds, the regeneration of C5 compounds from C6 compounds by carbon rearrangement, and an improved balance between regeneration of C5 compounds and lower glycolysis. In turn, this allows the engineered microorganism to generate sufficient quantities of metabolic precursors (e.g., acetyl-CoA) which can be used in a bioproduct pathway, and the engineered cells can include further modifications to those pathway enzymes allowing for production of a desired bioproduct.

公开(公告)号：US12043859B2

公开(公告)日：2024-07-23

申请号：US17426973

申请日：2020-01-30

Applicant: Genomatica, Inc.

Inventor： Lauri H. Suominen ,  Jefferson Lievense ,  Aiguo Liu ,  Jorge Jesus Martinez-Gonzalez ,  Myong Kon Ko ,  Wei Nan

IPC: C12P7/22 ,  B01D11/02 ,  C12M1/26

CPC classification number: C12P7/22 ,  B01D11/0288 ,  B01D11/0292 ,  C12M33/10

Abstract: Methods of recovering cannabinoids from cell cultures include methods comprising steps of separating the cell culture at a temperature above the melting point of the cannabinoid to separate a light phase comprising liquid state cannabinoid from a heavy phase; and methods comprising treating the cell culture at a temperature below the melting point of the cannabinoid to separate a light phase from a heavy phase comprising solid state cannabinoid. Other methods include contacting the culture with a water-miscible solvent to form a water-miscible phase and an aqueous phase, separating the two phases and recovering the cannabinoid. Other methods include contacting the culture with a water-immiscible solvent to form a water-immiscible phase and an aqueous phase, separating the two phases, and recovering the cannabinoid. Other methods include washing the inner surface of a fermentation vessel with alkaline solution to recover cannabinoid attached to the vessel surface. Various methods make use of aqueous solvent systems comprising no organic solvent, aqueous solvent systems comprising added water-miscible organic solvent, and dual-phase aqueous/water-immiscible solvent systems.

公开(公告)号：US20240225971A1

公开(公告)日：2024-07-11

申请号：US18569956

申请日：2022-06-10

Applicant: Genomatica, Inc.

Inventor： Kyle HUSTON

IPC: A61K8/34 ,  A61Q13/00

CPC classification number: A61K8/345 ,  A61Q13/00

Abstract: The disclosure provides embodiments directed to a composition comprising a 1,3-butylene glycol (1,3-BG; such as but not limited to, bioBG) and one or more fragrance ingredients, which form a fragrance component of the composition. 1,3-BG demonstrates exemplary qualities and characteristics of being natural, sustainable, and substantially non-odorous, for use in fragrance compositions. Methods of using or applying such 1,3-BG fragrance compositions provide a cost-efficient, sustainable composition, since 1,3-BG does not mask or alter the fragrance notes of the fragrance ingredients allowing for the use of smaller quantities of fragrance ingredients. The 1,3-BG fragrance compositions of the disclosure also improve fragrance performance and reduces greenhouse gases, while avoiding any irritation, sensitization, or allergic reaction in humans.

公开(公告)号：US20240190798A1

公开(公告)日：2024-06-13

申请号：US18469170

申请日：2023-09-18

Applicant: Genomatica, Inc.

Inventor： Julia Khandurina ,  Rachel Diane Pacheco ,  Lauri Hannunpoika Suominen ,  Rainer Wagester

IPC: C07C29/80 ,  B01D3/14 ,  B01D15/36 ,  C07C29/94 ,  C07C31/20

CPC classification number: C07C29/80 ,  B01D3/143 ,  B01D15/361 ,  C07C29/94 ,  B01D2202/20 ,  C07C31/207

Abstract: Provided herein are bioderived 1,3-butanediol compositions and systems and processes for producing such bioderived 1,3-butanediol compositions.

公开(公告)号：US11981952B2

公开(公告)日：2024-05-14

申请号：US16915454

申请日：2020-06-29

Applicant: Genomatica, Inc.

Inventor： Andreas W. Schirmer ,  Haibo Wang ,  Stephen B. Del Cardayre ,  Zhihao Hu ,  Louis G. Hom ,  Baolong Zhu ,  Cindy Chang ,  Emanuela E. Popova

IPC: C12P7/6409 ,  C12N1/21 ,  C12N9/02 ,  C12N9/04 ,  C12N15/52 ,  C12N15/62 ,  C12P7/18 ,  C12P7/42 ,  C12P7/6436

CPC classification number: C12P7/6409 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0071 ,  C12N9/0077 ,  C12N15/52 ,  C12N15/62 ,  C12P7/18 ,  C12P7/42 ,  C12P7/6436 ,  C12Y114/14001 ,  C12Y114/15003 ,  C07K2319/00 ,  C12Y101/00 ,  C12Y102/01042 ,  Y02E50/10

Abstract: The disclosure relates to omega-hydroxylated fatty acid derivatives and methods of producing them. Herein, the disclosure encompasses a novel and environmentally friendly production method that provides omega-hydroxylated fatty acid derivatives at high purity and yield. Further encompassed are recombinant microorganisms that produce omega-hydroxylated fatty acid derivatives through selective fermentation.

公开(公告)号：US20240052332A1

公开(公告)日：2024-02-15

申请号：US18266238

申请日：2021-12-08

Applicant: Genomatica, Inc.

Inventor： Michael A. Noble ,  Sarah Skye McNees ,  Darlene Jan Abcede Usi ,  Joseph R. Warner

IPC: C12N9/88 ,  C12P7/42

CPC classification number: C12N9/88 ,  C12Y404/01026 ,  C12P7/42

Abstract: Described herein are non-natural olivetolic acid cyclases (OAC) variants capable of forming a 2,4-dihydroxy-6-alkylbenzoic acid from a 3,5,7-trioxoacyl-CoA or a 3,5,7-trioxocarboxylate substrate at a greater rate than a wild type or control OAC. The non-natural OAC (and OLS) can be expressed in an engineered cell having a pathway to form cannabinoids, which include CBGA, its analogs and derivatives. CBGA can be used for the preparation of cannabigerol (CBG), which can be used in therapeutic compositions.

公开(公告)号：US20230399688A1

公开(公告)日：2023-12-14

申请号：US18120951

申请日：2023-03-13

Applicant: GENOMATICA, INC.

Inventor： Stephanie CULLER ,  Ihsiung Brandon CHEN ,  Priti PHARKYA ,  Steve VAN DIEN ,  Nelson BARTON

IPC: C12Q1/6869 ,  A61K35/66 ,  C12P21/02 ,  C12P19/34 ,  A61K36/02 ,  A61K36/06 ,  C12N5/16 ,  C12N15/09 ,  C40B40/04

CPC classification number: C12Q1/6869 ,  A61K35/66 ,  C12P21/02 ,  C12P19/34 ,  A61K36/02 ,  A61K36/06 ,  C12N5/16 ,  C12N15/09 ,  C40B40/04 ,  Y02A50/30 ,  A61K2236/15 ,  A61K2236/30 ,  C12N2330/50

Abstract: In alternative embodiments, provided herein are transcription/translation (TX-TL) systems and methods of using them for use as rapid prototyping platforms for the synthesis, modification and identification of natural products (NPs), and natural product analogs (NPAs) and secondary metabolites, from biosynthetic gene cluster pipelines. In alternative embodiments, exemplary TX-TL systems as provided herein are used for the combinatorial biosynthesis of natural products (NPs), natural product analogs (NPAs) and secondary metabolites. In alternative embodiments, exemplary TX-TL systems as provided herein are used for the rapid prototyping of complex biosynthetic pathways as a way to rapidly assess combinatorial and biosynthetic designs before moving to cellular hosts. In alternative embodiments, these exemplary TX-TL systems are multiplexed for high-throughput (HT) automation and for prototyping engineered platforms for the synthesis or modification of natural products (NPs), and natural product analogs (NPAs) and secondary metabolites analogs.

公开(公告)号：US20230332193A1

公开(公告)日：2023-10-19

申请号：US18043258

申请日：2021-09-01

Applicant: GENOMATICA, INC.

Inventor： Andreas W. SCHIRMER ,  Deqiang ZHANG ,  Jamison Parker HUDDLESTON

IPC: C12P17/06 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12N15/52

CPC classification number: C12P17/06 ,  C12N9/0006 ,  C12N9/1029 ,  C12Y203/01206 ,  C12N9/88 ,  C12Y404/01026 ,  C12N9/1085 ,  C12Y205/0101 ,  C12N15/52

Abstract: The disclosure relates to a non-natural flavin-dependent oxidase comprising at least one amino acid variation as compared to a wild type flavin-dependent oxidase, wherein the non-natural flavin-dependent oxidase does not comprise a disulfide bond, and wherein the non-natural flavin-dependent oxidase is capable of oxidative cyclization of a prenylated aromatic compound into a cannabinoid. The disclosure also relates to a nucleic acid, an expression construct, and an engineered cell for making the non-natural flavin-dependent oxidase. Also provided are compositions comprising the non-natural flavin-dependent oxidase; isolated non-natural flavin-dependent oxidase and methods of making the same; cell extracts comprising the non-natural flavin-dependent oxidase; and methods of making cannabinoids.

公开(公告)号：US11708589B2

公开(公告)日：2023-07-25

申请号：US16894600

申请日：2020-06-05

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Mark J. Burk ,  Robin E. Osterhout ,  Jun Sun ,  Priti Pharkya

IPC: C12P7/18 ,  C12N15/52

CPC classification number: C12P7/18 ,  C12N15/52

Abstract: Provided herein is a non-naturally occurring microbial organism having a 1,3-butanediol (1,3-BDO) pathway and comprising at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. In some embodiments, the pathway includes reducing equivalents from CO or hydrogen. In certain embodiments, a 1,3-BDO pathway proceeds by way of central metabolites pyruvate, succinate or alpha-ketoglutarate. Also provided herein is a method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO.