公开(公告)号：US20240139253A1

公开(公告)日：2024-05-02

申请号：US18129398

申请日：2023-03-31

Applicant: Wake Forest University Health Sciences ,  Emory University ,  Children's Healthcare of Atlanta, Inc.

Inventor： Maria Graca N.D. Almeida-Porada ,  Christopher D. Porada ,  Anthony Atala ,  Christopher B. Doering ,  H. Trent Spencer

IPC: A61K35/28 ,  A61K38/37 ,  C12N5/0775

CPC classification number: A61K35/28 ,  A61K38/37 ,  C12N5/0663 ,  C12N5/0668 ,  C12N2510/00

Abstract: Disclosed herein are method of treating hemophilia A in a subject comprising injecting the subject with mesenchymal stromal/stem cells (MSC) modified to express high levels of Factor VIII protein. The MSC are isolated prenatally, at birth, or after the subject's birth. The modified MSC may also express high levels von Willebrand factor protein.

公开(公告)号：US11806445B2

公开(公告)日：2023-11-07

申请号：US16947053

申请日：2020-07-16

Applicant: Wake Forest University Health Sciences

Inventor： Anthony Atala ,  Gayoung Jeong ,  James J. Yoo ,  Sang Jin Lee ,  Young-Joon Seol

IPC: A61F2/10 ,  A61L27/60 ,  B33Y80/00 ,  B33Y70/00 ,  C08L5/08 ,  A61L27/20 ,  C08L89/06 ,  C12N5/071 ,  B29C64/106 ,  B33Y10/00 ,  A61L27/18 ,  A61L27/38 ,  A61L27/52 ,  G01N33/50 ,  B29K75/00 ,  B29K105/00

CPC classification number: A61L27/60 ,  A61F2/10 ,  A61L27/18 ,  A61L27/20 ,  A61L27/3804 ,  A61L27/3813 ,  A61L27/3839 ,  A61L27/3891 ,  A61L27/52 ,  B29C64/106 ,  B33Y10/00 ,  B33Y70/00 ,  B33Y80/00 ,  C08L5/08 ,  C08L89/06 ,  C12N5/0698 ,  G01N33/502 ,  G01N33/5044 ,  A61F2210/0076 ,  A61F2240/001 ,  A61L2300/64 ,  A61L2430/34 ,  B29K2075/00 ,  B29K2105/0061 ,  C12N2502/091 ,  C12N2502/092 ,  C12N2502/094 ,  C12N2502/1305 ,  C12N2502/1323 ,  C12N2533/30 ,  C12N2533/54 ,  C12N2533/80

Abstract: Provided are live, artificial, skin substitute products and methods of making and using the same, such as for wound treatment and compound testing, including compound testing for efficacy, toxicity, penetration, irritation and/or metabolism testing of drug candidates or compositions such as cosmetics.

公开(公告)号：US11759579B2

公开(公告)日：2023-09-19

申请号：US16696738

申请日：2019-11-26

Applicant: Wake Forest University Health Sciences

Inventor： James J. Yoo ,  Anthony Atala ,  Kyle W. Binder ,  Mohammad Z. Albanna ,  Weixin Zhao ,  Dennis Dice ,  Tao Xu

IPC: A61M5/178 ,  A61M11/00 ,  A61B5/00 ,  A61B5/107 ,  A61L27/22 ,  A61L27/24 ,  A61L27/38 ,  A61L27/60 ,  C12N5/077 ,  A61M35/00 ,  A61M1/00 ,  A61M5/00 ,  A61M5/315 ,  A61M27/00 ,  B41J3/407 ,  A61B17/00 ,  A61B17/322 ,  A61B90/50 ,  A61B34/30 ,  A61B34/10

CPC classification number: A61M11/00 ,  A61B5/0064 ,  A61B5/1077 ,  A61B5/1079 ,  A61B5/445 ,  A61B5/6835 ,  A61L27/225 ,  A61L27/24 ,  A61L27/3813 ,  A61L27/60 ,  A61M35/20 ,  C12N5/0656 ,  A61B34/30 ,  A61B90/50 ,  A61B2017/00969 ,  A61B2017/3225 ,  A61B2034/105 ,  B41J3/4073

Abstract: Provided herein is a delivery system, including: (a) an optical sensor configured to detect data to create a map of a patient bodily surface; and (b) a dispenser operatively associated with the optical sensor and configured to deliver compositions (optionally including cells) to the patient bodily surface based upon the data or map. Methods of forming a tissue on a patient bodily surface of a patient in need thereof are also provided, as are methods, systems and computer program products useful for processing patient bodily surface data.

公开(公告)号：US11732241B2

公开(公告)日：2023-08-22

申请号：US15768293

申请日：2016-10-14

Applicant: Wake Forest University Health Sciences

Inventor： Anthony Atala ,  Colin Bishop ,  Ivy Mead

IPC: C12N5/071 ,  C12M3/00 ,  G01N33/50 ,  B33Y10/00

CPC classification number: C12N5/0671 ,  B33Y10/00 ,  C12M21/08 ,  C12N5/067 ,  G01N33/5088 ,  C12N2502/14 ,  C12N2503/02

Abstract: Provided herein are cell compositions useful for making artificial liver constructs. The cell composition my include, in combination, (a) hepatocyte cells, (b) Kuppfer cells, (c) hepatic stellate cells, (d) sinusoidal endothelial cells, and (e) cholangiocyte cells.

公开(公告)号：US20220106572A1

公开(公告)日：2022-04-07

申请号：US17643441

申请日：2021-12-09

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： Robert T. Wicks ,  Anthony Atala ,  Goodwell Nzou ,  Elizabeth E. Wicks

IPC: C12N5/071 ,  C12N5/00

Abstract: Provided herein is an in vitro model of the blood brain barrier. In some embodiments, the model includes: an endothelial cell layer, and brain tissue layer comprising neuronal cells, and optionally one or more of astrocytes, pericytes, oligodendrocytes, and microglia. In some embodiments, the model further comprises a porous membrane between said endothelial cell layer and the neuronal cell layer. A microfluidic device comprising the same and methods of use thereof are also provided.

公开(公告)号：US20220010270A1

公开(公告)日：2022-01-13

申请号：US17449015

申请日：2021-09-27

Applicant: Wake Forest University Health Sciences

Inventor： Sittadjody Sivanandane ,  Russel C. Sequeira ,  John D. Jackson ,  Anthony Atala ,  James J. Yoo

IPC: C12N5/075

Abstract: A method of providing a culture of oogonia stem cells comprising oogonia stem cells is provided. The method may further include culturing the oogonia stem cells with granulosa and theca cells to differentiate the oogonia stem cells into oocytes. In some embodiments, the culturing comprises including the oogonia stem cells in an in vitro follicle construct or a microcapsule comprising said granulosa and theca cells. Further described herein is a method of forming a bioengineered follicle construct capable of releasing a mature oocyte. An in vitro fertilization method using the mature oocyte is also provided.

公开(公告)号：US20210324311A1

公开(公告)日：2021-10-21

申请号：US16341521

申请日：2017-10-10

Applicant: Wake Forest University Health Sciences

Inventor： Aleksander Skardal ,  Thomas Shupe ,  Anthony Atala

IPC: C12M3/00 ,  C12M3/06 ,  C12M1/00 ,  G01N33/50

Abstract: Described herein are apparatus, including multi-tissue body-on-a-chip apparatus. In some embodiments, described are apparatus including at least a first, second, and third chamber in fluid communication with one another and with at least one tissue in each chamber, such as, for example, liver tissue in said first chamber; cardiac muscle tissue in said second chamber; and lung tissue in said third chamber; and a common aqueous growth media in said first, second, and third chamber. In some embodiments, the apparatus includes a fourth chamber including testicular or ovarian tissue in said fourth chamber. In some embodiments, the apparatus includes an optional fifth or sixth chamber; each said chamber includes different additional tissue selected from the group consisting of vascular endothelial, skeletal muscle, kidney, nerve, brain, and intestinal tissue (e.g., small intestine tissue and/or colon tissue). Also described are methods of using an apparatus of the present invention.

公开(公告)号：US11135248B2

公开(公告)日：2021-10-05

申请号：US15829488

申请日：2017-12-01

Applicant: Wake Forest University Health Sciences

Inventor： Yuanyuan Zhang ,  Anthony Atala

IPC: C12N5/071 ,  A61K35/22 ,  C12N5/0775

Abstract: Provided herein are stem cells and methods for producing a culture of stem cells from urine. The stem cells may be differentiated into an osteogenic, chondrogenic, adipogenic, endothelial, neurogenic or myogenic lineage. Methods of use of the cells are provided, including methods of treating a subject in need of a cell based therapy.

公开(公告)号：US20210269943A1

公开(公告)日：2021-09-02

申请号：US17316342

申请日：2021-05-10

Applicant: Wake Forest University Health Sciences

Inventor： Sang Jin Lee ,  James J. Yoo ,  Young Min Ju ,  Anthony Atala

IPC: D01D5/00 ,  A61K35/44 ,  C07K16/46 ,  A61L27/24 ,  A61K31/737 ,  A61K38/36 ,  A61K38/18 ,  A61K31/729 ,  A61K38/39 ,  C07K16/28 ,  C07D315/00 ,  A61K31/728 ,  D01F6/92 ,  C07K16/18 ,  D01F1/10

Abstract: The invention is directed to products and methods for preparing self-seeding vascular constructs generated as a bi-layered electrospun matrices, conjugated with EPC-specific antibodies and anti-thrombogenic agents on the inner surfaces of their lumens.

公开(公告)号：US11013828B2

公开(公告)日：2021-05-25

申请号：US15938706

申请日：2018-03-28

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： Anthony Atala ,  James Yoo ,  In Kap Ko

IPC: A61L27/36 ,  A61K38/18 ,  A61L27/18 ,  A61L27/24 ,  A61L27/38 ,  A61K35/34 ,  A61K45/06 ,  A61K47/34 ,  A61K47/42 ,  A61L27/54 ,  C12N5/00 ,  C12N5/077 ,  A61K9/00

Abstract: The invention is directed to methods and compositions for obtaining uniform sized muscle fiber fragments for transplantation. These muscle fiber fragments are able to reconstitute into long fibers that are oriented along native muscle. The implanted muscle cells integrate with native vascular and neural network, as confirmed by histology and immunohistochemistry. This invention is particularly advantageous because autologous muscle can be harvested from a donor site, processed and injected into target sites in the operating room. The fragmented muscle fibers can be readily integrated within the host.