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    Copy number variation determination, methods and systems
    71.
    发明授权
    Copy number variation determination, methods and systems 有权
    复制数字变化确定，方法和系统

    公开(公告)号：US08450065B2

    公开(公告)日：2013-05-28

    申请号：US13307817

    申请日：2011-11-30

    Applicant: Ramesh Ramakrishnan

    Inventor： Ramesh Ramakrishnan

    IPC: C12Q1/68 ,  C12P19/34

    CPC classification number: C12Q1/6869 ,  C12Q1/6851 ,  C12Q2545/101 ,  C12Q2527/143

    Abstract: The present invention methods and systems for determining copy number variation of a target polynucleotide in a genome of a subject including amplification based techniques. Methods can include pre-amplification of the sample followed by distribution of sample and a plurality of reaction volumes, quantitative detection of a target polynucleotide and a reference polynucleotide, and analysis so as to determine the relative copy number of the target polynucleotide sequence in the genome of the subject.

    Abstract translation: 用于确定受试者的基因组中的靶多核苷酸的拷贝数变异的本发明的方法和系统，包括基于扩增的技术。 方法可以包括样品的预扩增，随后分布样品和多个反应体积，定量检测靶多核苷酸和参考多核苷酸，并进行分析，以确定基因组中靶多核苷酸序列的相对拷贝数 的主题。

    Method and system for microfluidic device and imaging thereof
    72.
    发明授权
    Method and system for microfluidic device and imaging thereof 有权
    微流体装置及其成像方法及系统

    公开(公告)号：US08367016B2

    公开(公告)日：2013-02-05

    申请号：US13360591

    申请日：2012-01-27

    Applicant: Emerson Chueng Quan ,  Colin Jon Taylor ,  Michael Lee ,  Christopher G. Ceasar ,  Greg Harris ,  Gang Sun

    Inventor： Emerson Chueng Quan ,  Colin Jon Taylor ,  Michael Lee ,  Christopher G. Ceasar ,  Greg Harris ,  Gang Sun

    IPC: G01N33/00

    CPC classification number: G01N33/00 ,  B01L3/5025 ,  B01L3/502707 ,  B01L2200/025 ,  B01L2200/12 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2400/0481 ,  B01L2400/0655 ,  Y10T436/11

    Abstract: A method for producing an image of an object within a chamber of a microfluidic device includes providing the microfluidic device having x, y, and z dimensions and a chamber depth center point located along the z dimension. The chamber depth center point is located a known z dimension distance from a fiducial marking embedded within the microfluidic device. The method also includes placing the microfluidic device within an imaging system that includes an optical device capable of detecting the fiducial marking. The optical device defines an optical path axially aligned with the z dimension and has a focal plane perpendicular to the optical path. When the focal plane is moved along the optical path, the fiducial marking is maximally detected when the focal plane is at the z depth in comparison to when the focal plane is not substantially in-plane with the z depth.

    Abstract translation: 用于在微流体装置的腔室内产生物体的图像的方法包括提供具有x，y和z尺寸的微流体装置以及沿着z维度定位的腔室中心点。 腔室深度中心点位于与嵌入在微流体装置内的基准标记的已知z维距离。 该方法还包括将微流体装置放置在包括能够检测基准标记的光学装置的成像系统内。 光学装置限定与z尺寸轴向对准的光路，并且具有垂直于光路的焦平面。 当焦平面沿着光路移动时，当焦平面与z深度基本上不在平面内时，当焦平面处于z深度时，基准标记被最大程度地检测。

    Copy Number Variation Determination, Methods and Systems
    74.
    发明申请
    Copy Number Variation Determination, Methods and Systems 有权
    复制数变异确定，方法和系统

    公开(公告)号：US20120282604A1

    公开(公告)日：2012-11-08

    申请号：US13307817

    申请日：2011-11-30

    Applicant: Ramesh Ramakrishnan

    Inventor： Ramesh Ramakrishnan

    IPC: C12Q1/68 ,  G01N21/64

    CPC classification number: C12Q1/6869 ,  C12Q1/6851 ,  C12Q2545/101 ,  C12Q2527/143

    Abstract: The present invention methods and systems for determining copy number variation of a target polynucleotide in a genome of a subject including amplification based techniques. Methods can include pre-amplification of the sample followed by distribution of sample and a plurality of reaction volumes, quantitative detection of a target polynucleotide and a reference polynucleotide, and analysis so as to determine the relative copy number of the target polynucleotide sequence in the genome of the subject.

    Abstract translation: 用于确定受试者的基因组中的靶多核苷酸的拷贝数变异的本发明的方法和系统，包括基于扩增的技术。 方法可以包括样品的预扩增，随后分布样品和多个反应体积，定量检测靶多核苷酸和参考多核苷酸，并进行分析，以确定基因组中靶多核苷酸序列的相对拷贝数 的主题。

    METHOD AND SYSTEM FOR CRYSTALLIZATION AND X-RAY DIFFRACTION SCREENING
    76.
    发明申请
    METHOD AND SYSTEM FOR CRYSTALLIZATION AND X-RAY DIFFRACTION SCREENING 有权
    用于结晶和X射线衍射筛选的方法和系统

    公开(公告)号：US20120021523A1

    公开(公告)日：2012-01-26

    申请号：US13168041

    申请日：2011-06-24

    Applicant: Brian Fowler ,  Andrew May

    Inventor： Brian Fowler ,  Andrew May

    IPC: G01N23/20

    CPC classification number: G01N23/20025

    Abstract: An integrated fluidic circuit includes a substrate layer and a first structure coupled to the substrate layer and including a plurality of channels. The first structure is configured to provide for flow of one or more materials through the plurality of channels. The integrated fluidic circuit also includes a second structure coupled to the substrate layer. The second structure includes a plurality of control channels configured to receive an actuation pressure. The integrated fluidic circuit is characterized by a thickness of less than 1.5 mm.

    Abstract translation: 集成流体回路包括衬底层和耦合到衬底层并且包括多个通道的第一结构。 第一结构被配置为提供通过多个通道的一种或多种材料的流动。 集成流体回路还包括耦合到衬底层的第二结构。 第二结构包括被配置为接收致动压力的多个控制通道。 集成流体回路的特征在于厚度小于1.5mm。

    Microfluidic devices and methods
    77.
    发明授权
    Microfluidic devices and methods 有权
    微流体装置和方法

    公开(公告)号：US08058630B2

    公开(公告)日：2011-11-15

    申请号：US12688462

    申请日：2010-01-15

    Applicant: Martin Pieprzyk ,  Geoff Facer ,  Timothy Woudenberg ,  Brian Fowler

    Inventor： Martin Pieprzyk ,  Geoff Facer ,  Timothy Woudenberg ,  Brian Fowler

    IPC: G01N21/64

    CPC classification number: G01N1/28 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/16 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0655 ,  B33Y80/00 ,  C12Q1/686 ,  G01N21/05 ,  G01N21/6452 ,  G01N2021/0346 ,  Y10T137/7793 ,  Y10T436/2575

    Abstract: Embodiments of the present invention provide improved microfluidic devices and related apparatus, systems, and methods. Methods are provided for reducing mixing times during use of microfluidic devices. Microfluidic devices and related methods of manufacturing are provided with increased manufacturing yield rates. Improved apparatus and related systems are provided for supplying controlled pressure to microfluidic devices. Methods and related microfluidic devices are provided for reducing dehydration of microfluidic devices during use. Microfluidic devices and related methods are provided with improved sample to reagent mixture ratio control. Microfluidic devices and systems are provided with improved resistance to compression fixture pressure induced failures. Methods and systems for conducting temperature controlled reactions using microfluidic devices are provided that reduce condensation levels within the microfluidic device. Methods and systems are provided for improved fluorescent imaging of microfluidic devices.

    Abstract translation: 本发明的实施例提供了改进的微流体装置和相关装置，系统和方法。 提供了减少使用微流体装置期间的混合时间的方法。 微流控装置和相关的制造方法具有提高的制造产率。 提供改进的装置和相关系统用于向微流体装置提供受控的压力。 提供了方法和相关的微流体装置，用于在使用过程中减少微流体装置的脱水。 微流控装置和相关方法提供了改进的样品与试剂混合比控制。 微流体装置和系统具有改进的抗压缩夹具压力引起的故障的阻力。 提供了使用微流体装置进行温度控制反应的方法和系统，其减少微流体装置内的冷凝水平。 提供了用于改进微流体装置的荧光成像的方法和系统。

    SELECTIVE TAGGING OF SHORT NUCLEIC ACID FRAGMENTS AND SELECTIVE PROTECTION OF TARGET SEQUENCES FROM DEGRADATION
    80.
    发明申请
    SELECTIVE TAGGING OF SHORT NUCLEIC ACID FRAGMENTS AND SELECTIVE PROTECTION OF TARGET SEQUENCES FROM DEGRADATION 审中-公开
    选择性标记短核酸片段和选择性保护目标序列从降解

    公开(公告)号：US20100285537A1

    公开(公告)日：2010-11-11

    申请号：US12752974

    申请日：2010-04-01

    Applicant: Bernhard G. Zimmermann

    Inventor： Bernhard G. Zimmermann

    IPC: C12P19/34 ,  C02F3/34

    CPC classification number: C12N15/1093 ,  C12N15/10 ,  C12N15/1065 ,  C12N15/1072 ,  C12Q1/6846 ,  C12Q2521/307 ,  C12Q2521/325 ,  C12Q2537/159

    Abstract: Methods are provided for selective tagging of short nucleic acids comprising a short target nucleotide sequence over longer nucleic acids comprising the same target nucleotide sequence. The methods can involve performing one or two cycles of amplification of a sample comprising long nucleic acids and short nucleic acids, each comprising the same target nucleotide sequence with at least two target-specific primers or primer pairs under suitable annealing conditions, wherein the primer pairs comprise: an inner primer or primer pair that can amplify the target nucleotide sequence on long and short nucleic acids (wherein each inner primer comprises a 5′ nucleotide tag; and an outer primer or primer pair that amplifies the target nucleotide sequence on long nucleic acids, but not on short nucleic acids); whereby the amplification after a second cycle produces at least one tagged target nucleotide sequence that comprises two nucleotide tags, one from each inner primer, with the target nucleotide sequence located between the nucleotide tags.

    Abstract translation: 提供了用于在包含相同靶核苷酸序列的较长核酸上选择性标记包含短目标核苷酸序列的短核酸的方法。 所述方法可以包括在合适的退火条件下进行包含长核酸和短核酸的样品的一个或两个循环的扩增，每个循环包含相同的靶核苷酸序列与至少两个靶特异性引物或引物对，其中引物对 包括：可以扩增长和短核酸上的靶核苷酸序列的内部引物或引物对（其中每个内引物包含5'核苷酸标签;以及扩增长核酸上的靶核苷酸序列的外引物或引物对 ，但不在短核酸上）; 由此在第二周期之后的扩增产生至少一个标记的靶核苷酸序列，其包含两个核苷酸标签，每个内引物中的一个，靶核苷酸序列位于核苷酸标签之间。

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