公开(公告)号：US20060035372A1

公开(公告)日：2006-02-16

申请号：US11203379

申请日：2005-08-15

申请人： George Kouns

发明人： George Kouns

IPC分类号： C12M1/42 ,  C12N5/08

CPC分类号： C12N5/0696 ,  C12M23/10 ,  C12M35/02 ,  C12N2506/00 ,  C12N2529/00

摘要： A method for the production and use of multipotential stem-like cells is disclosed. The preparation utilized in this method is characterized by the contact of low level electrical currents with cultures of fibroblasts or other -blast cells enriched by fibroblast growth factor and other nutrients. The electrical current is conducted by means of silver electrode(s) brought into contact with the fibroblast preparation or other -blast cell preparation cultured for that purpose. The cells of the preparation may be used in applications that require the use of stem cells, including therapeutic applications, without the need for human fetuses or human umbilical cords or penetrating human bones to extract bone marrow. The cells thus produced have the ability to redifferentiate into endoderm, ectoderm and mesoderm to form any tissue of the body except the lens of the eye. Any cell found in the blood may be copied and multiplied. Any tissue of the body may be copied and multiplied with the lone exception of the lens of the eye as noted above.

摘要翻译： 公开了一种生产和使用多潜能茎状细胞的方法。 该方法中使用的制剂的特征在于低水平电流与成纤维细胞或成纤维细胞生长因子和其他营养物质富集的其他细胞培养物的接触。 电流通过与成纤维细胞制剂接触的银电极或为此目的而培养的其它细胞培养基进行。 制剂的细胞可以用于需要使用干细胞的应用，包括治疗应用，而不需要人胎儿或人脐带或穿透人骨来提取骨髓。 由此产生的细胞具有再分化成内胚层，外胚层和中胚层以形成除了眼睛的晶状体之外的身体的任何组织的能力。 血液中发现的任何细胞都可能被复制和繁殖。 如上所述，身体的任何组织可能被复制并乘以眼睛的镜片的单独例外。

公开(公告)号：US20050112759A1

公开(公告)日：2005-05-26

申请号：US10872577

申请日：2004-06-21

申请人： Milica Radisic ,  Hyoungshin Park ,  Robert Langer ,  Lisa Freed ,  Gordana Vunjak-Novakovic

发明人： Milica Radisic ,  Hyoungshin Park ,  Robert Langer ,  Lisa Freed ,  Gordana Vunjak-Novakovic

IPC分类号： A61F2/00 ,  A61N20060101 ,  C12N5/00 ,  C12N5/077 ,  C12N5/08

CPC分类号： C12N5/0658 ,  C12N2502/02 ,  C12N2502/1358 ,  C12N2529/00

摘要： The present invention provides new methods for the in vitro preparation of bioartificial tissue equivalents and their enhanced integration after implantation in vivo. These methods include submitting a tissue construct to a biomimetic electrical stimulation during cultivation in vitro to improve its structural and functional properties, and/or in vivo, after implantation of the construct, to enhance its integration with host tissue and increase cell survival and functionality. The inventive methods are particularly useful for the production of bioartificial equivalents and/or the repair and replacement of native tissues that contain electrically excitable cells and are subject to electrical stimulation in vivo, such as, for example, cardiac muscle tissue, striated skeletal muscle tissue, smooth muscle tissue, bone, vasculature, and nerve tissue.

摘要翻译： 本发明提供了体外制备生物人造组织当量的新方法及其在体内植入后的增强整合。 这些方法包括在体外培养过程中将组织构建物递送至仿生电刺激，以改善其结构和功能性质，和/或在植入该构建体后体内，以增强其与宿主组织的整合并增加细胞存活和功能。 本发明的方法特别可用于生产生物人造等同物和/或修复和替换含有电可兴奋细胞的天然组织，并且在体内受到电刺激，例如心肌组织，条纹骨骼肌组织 ，平滑肌肉组织，骨骼，脉管系统和神经组织。

公开(公告)号：US20240279614A1

公开(公告)日：2024-08-22

申请号：US18652960

申请日：2024-05-02

申请人： University of Science and Technology of China

发明人： Gang ZHAO ,  Conghui TIAN ,  Lingxiao SHEN

IPC分类号： C12N5/071 ,  A01N1/02

CPC分类号： C12N5/0682 ,  A01N1/0221 ,  A01N1/0231 ,  A01N1/0284 ,  C12N2500/25 ,  C12N2501/11 ,  C12N2501/235 ,  C12N2501/31 ,  C12N2529/00 ,  C12N2531/00

摘要： Methods for cryopreservation and resuscitation of follicles are provided by the present disclosure, relating to the technical field of follicular cryopreservation. According to the method for cryopreserving follicles provided by the present disclosure, follicles are firstly encapsulated with Alginate hydrogel, and incubated in protectant A and B. and moved into a protective solution A for incubation. Follicles encapsulated in hydrogel microcapsules are then loaded into straws and immediately submerged into liquid nitrogen for cryopreservation. Then, they are rapidly warmed by a 37° C. water bath combined with nano-warming technique. To verify the preservation effect, the warmed preantral follicles are further cultured in vitro in three dimensions.

公开(公告)号：US11965179B1

公开(公告)日：2024-04-23

申请号：US17353388

申请日：2021-06-21

申请人： Fariborz Izadyar

发明人： Fariborz Izadyar

IPC分类号： C12N5/078 ,  A61K9/00 ,  A61K35/19 ,  C12N11/04 ,  C12N13/00

CPC分类号： C12N5/0644 ,  A61K9/0019 ,  A61K9/0095 ,  A61K35/19 ,  C12N11/04 ,  C12N13/00 ,  C12N2509/00 ,  C12N2529/00

摘要： A method of creating a pooled platelet lysate product may include obtaining whole blood from a source; separating platelets from the whole blood using a pressurized rotating microfluidic filtration system; creating platelet rich plasma (PRP) by concentrating the platelets in plasma and removing red blood cells and white blood cells using centrifugation; centrifuging the PRP, removing the plasma, and re-suspending the PRP in the lactated ringers solution (LRS) to create concentrated PRP (C-PRP); laser activating the C-PRP to create an activated C-PRP product; freezing and thawing the activated C-PRP product at least 3 times, wherein vortexing and sonication are performed after each thaw; and reconstituting the activated C-PRP in a saline to create a platelet lysate product with a known potency.

公开(公告)号：US20240076619A1

公开(公告)日：2024-03-07

申请号：US18361584

申请日：2023-07-28

申请人： Agilent Technologies, Inc.

发明人： Xiaoyu Zhang ,  Yama A. Abassi ,  Nan Li ,  Xiaobo Wang

IPC分类号： C12N5/077 ,  C12N5/00 ,  C12N5/074 ,  C12N13/00 ,  G01N27/02

CPC分类号： C12N5/0657 ,  C12N5/0018 ,  C12N5/0696 ,  C12N13/00 ,  G01N27/028 ,  C12N2506/45 ,  C12N2529/00

摘要： A method of characterizing an effect of a compound on cardiomyocyte beating, including providing a system configured to culture, electrically pace, and monitor heating of beating cells: culturing two populations of immature cardiomyocytes in the system; adding a compound suspected of having an effect on cardiomyocyte maturation to one of the populations of immature cardiomyocytes; electrically pacing the two populations of immature cardiomyocytes according to a pulse profile that functionally matures immature cardiomyocytes until at least one of the two populations of cardiomyocytes is functionally mature; and Characterizing the compound as further driving maturation if the population with compound addition functionally matures before the other cardiomyocyte population.

公开(公告)号：US11859175B2

公开(公告)日：2024-01-02

申请号：US16082926

申请日：2016-08-09

申请人： STEMON INC.

发明人： Soon Hag Kim

IPC分类号： C12N5/00 ,  C12N13/00 ,  C12N5/0797 ,  C12N5/077 ,  C12N5/071

CPC分类号： C12N13/00 ,  C12N5/067 ,  C12N5/0623 ,  C12N5/0656 ,  C12N5/0658 ,  C12N2521/10 ,  C12N2523/00 ,  C12N2529/00

摘要： The present invention relates to a cell reprogramming method using a physical stimulation-mediated environmental influx, and more specifically, by subjecting differentiated or non-differentiated cells to physical stimulation which can promote an environmental influx, such as ultrasonic waves, laser or heat shock, without the introduction of a reprogramming-inducing factor or a chemical substance to the differentiated cells, the cells can be reprogrammed with just the imposition of an external environmental influx into pluripotent cells or arbitrary differentiated cells having a different expression type from the differentiated or non-differentiated cells, and as such an inducement has a simple and effective production process, the possibility of an autogenic cell therapy can be made greater.

公开(公告)号：US20230355968A1

公开(公告)日：2023-11-09

申请号：US18027824

申请日：2021-09-22

申请人： Virginia Tech Intellectual Properties, Inc.

发明人： Rafael V. Davalos ,  Irving C. Allen ,  Scott S. Verbridge ,  Nastaran Alinezhadbalalami

IPC分类号： A61N1/32 ,  A61N1/36 ,  C12N5/0783

CPC分类号： A61N1/327 ,  A61N1/36002 ,  C12N5/0638 ,  C12N2529/00 ,  C12N2502/1121

摘要： Expansion of cytotoxic T lymphocytes (CTLs) is a crucial step in almost all cancer immunotherapeutic methods. Current techniques for expansion of tumor-reactive CTLs present major limitations. The present invention comprises a novel method to effectively produce and expand tumor-activated CTLs using high-voltage pulsed electric fields. Tumor cells were subjected to high-frequency irreversible electroporation (HFIRE) with various electric field magnitudes and pulse widths, or irreversible electroporation (IRE). The treated tumor cells were subsequently cocultured with CD8+ cytotoxic T cells along with antigen-presenting cells. Tumor-activated CTLs can be produced and expanded when exposed to treated tumor cells. The activated CTLs produced with the present invention could be used for clinical applications with the goal of targeting and eliminating tumors.

公开(公告)号：US11654159B2

公开(公告)日：2023-05-23

申请号：US16092119

申请日：2017-04-06

申请人： UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.

发明人： Maria A. Grant ,  Tatiana E. Salazar ,  Huisheng Xie

IPC分类号： A61K35/28 ,  A61H39/00 ,  A61N1/20 ,  A61N1/36 ,  A61H39/08 ,  A61N1/05 ,  A61N1/18 ,  A61P29/00 ,  A61K9/00 ,  A61K38/19 ,  A61K38/20 ,  C12N5/0775

CPC分类号： A61K35/28 ,  A61H39/002 ,  A61H39/086 ,  A61K9/0019 ,  A61K38/193 ,  A61K38/20 ,  A61N1/0502 ,  A61N1/18 ,  A61N1/20 ,  A61N1/3616 ,  A61N1/36017 ,  A61P29/00 ,  C12N5/0665 ,  A61H2203/03 ,  C12N2529/00

摘要： Stimulation using EA of LI-4, LI-11, GV-14 and GV-20 in humans, horses, and rodents results in mobilization of MSCs into systemic circulation. Methods are provided for increasing mesenchymal stem cells in the circulating blood of a mammal by contacting acupuncture points LI-4, LI-11, GV-14, and GV-20 of the mammal with a therapeutically effective amount of EA stimulation to mobilize MSCs into the circulating blood of the mammal. Methods for treating damaged tissue, specifically damaged tendons are also provided. Isolated mesenchymal stem cells made according to these methods, methods of isolated them, and stem cell banks that store them are also provided.

公开(公告)号：US20230151386A1

公开(公告)日：2023-05-18

申请号：US17912928

申请日：2021-03-25

申请人： St Andrews Pharmaceutical Technology Ltd.

发明人： William J. Henry ,  Anna Montali ,  Jean-Christophe Bourdon

IPC分类号： C12N15/85 ,  C12M1/42

CPC分类号： C12N15/85 ,  C12M35/02 ,  C12N2310/10 ,  C12N2529/00

摘要： A method and apparatus for improving transfection efficiency in eukaryotic cells including the steps of providing a transfection mixture including an agent associated with at least one amphiphilic construct suitable for transfection. Adding the transfection mixture to one or more eukaryotic cells to form a transfection complex and allowing the transfection complex to undergo a transfection process to form one or more transfected cells. The method also includes the step of directing pulsed electromagnetic signals provided at any or any combination of a pre-determined frequency, at a pre-determined pulse rate, or at a pre-determined power, at the transfection mixture at step (a) prior to creating the transfection complex, at the transfection complex in step (b), at the transfection complex in step (c) and/or at the transfected cell complex after step (c).

公开(公告)号：US20180148692A1

公开(公告)日：2018-05-31

申请号：US15837366

申请日：2017-12-11

申请人： University of Cincinnati

发明人： Yi-Gang Wang ,  Ha Won Kim ,  Ruilian Ma

IPC分类号： C12N5/077 ,  A61K35/34 ,  C12N5/074

CPC分类号： C12N5/0657 ,  A61K35/34 ,  C12N5/0696 ,  C12N2506/45 ,  C12N2529/00

摘要： Methods of generating cardiomyocytes from induced pluripotent stem cells (IPSCs) are provided. More specifically, the present disclosure relates to methods of generating cardiomyocytes from iPSCs using electrical stimulation. In some aspects, uses of such cells for therapeutics and in methods of treatment are provided.