公开(公告)号：US20120104247A1

公开(公告)日：2012-05-03

申请号：US13262217

申请日：2009-03-31

Applicant: Kiyoshi Ogawa ,  Mitsutoshi Setou

Inventor： Kiyoshi Ogawa ,  Mitsutoshi Setou

IPC: H01J49/26

CPC classification number: H01J49/0004 ,  G01N1/06 ,  G01N1/286 ,  H01J49/0459

Abstract: A sample stage (2) on which a sample (4) is placed can be reciprocally moved along a guide (5) by a driving mechanism (6). A cutter (9) which is moved in an X-Y plane by a driving mechanism (10) is placed at a sample cutting position (B). When the sample stage (2) is moved to the sample cutting position (B) and the cutter is driven with the height of the sample stage (2) being appropriately adjusted, an upper portion of the sample 4 is horizontally cut off with a predetermined thickness and a new sample analysis surface which was inside the sample 4 is exposed. Hence, by repeating a mass analysis for a predetermined measurement area at an analysis position (C) and a partial cutting of the sample 4 at the sample cutting position (B), it is possible to achieve a three-dimensional mass analysis imaging of the sample (4) without removing the sample (4) from the sample stage (2).

Abstract translation: 其上放置有样品（4）的样品台（2）可以通过驱动机构（6）沿引导件（5）往复运动。 通过驱动机构（10）在X-Y平面中移动的切割器（9）被放置在样品切割位置（B）。 当样品台（2）移动到样品切割位置（B）并且在适当调节样品台（2）的高度的情况下驱动切割器时，样品4的上部以预定的 厚度和样品4内部的新的样品分析表面露出。 因此，通过在样本切断位置（B）的分析位置（C）和样本4的部分切割重复对于预定测量区域的质量分析，可以实现三维质量分析成像 样品（4），而不从样品台（2）中移除样品（4）。

公开(公告)号：US08134124B2

公开(公告)日：2012-03-13

申请号：US12446420

申请日：2007-10-22

Applicant: Jeff Blackwood ,  Stacey Stone

Inventor： Jeff Blackwood ,  Stacey Stone

IPC: G01N1/28 ,  G01R31/305

CPC classification number: G01N1/28 ,  G01N1/06 ,  G01N1/08 ,  G01N1/32 ,  G01N23/04 ,  H01J37/06 ,  H01J37/18 ,  H01J37/20 ,  H01J37/26 ,  H01J37/28 ,  H01J37/3056 ,  H01J2237/063 ,  H01J2237/182 ,  H01J2237/204 ,  H01J2237/208 ,  H01J2237/2802 ,  H01J2237/31745 ,  H01J2237/31749

Abstract: An improved method and apparatus for S/TEM sample preparation and analysis. Preferred embodiments of the present invention provide improved methods for TEM sample creation, especially for small geometry (

Abstract translation: 一种用于S / TEM样品制备和分析的改进方法和装置。 本发明的优选实施例提供用于TEM样品制备的改进方法，特别是对于小几何（<100nm厚）TEM薄片。 新颖的样品结构和铣削图案的新颖应用允许产生薄至50nm的S / TEM样品，而没有显着的弯曲或翘曲。 本发明的优选实施方案提供了部分或全部自动化TEM样品制备的方法，使TEM样品的创建和分析过程减少劳动密集度，并提高TEM分析的产量和再现性。

公开(公告)号：US08110146B2

公开(公告)日：2012-02-07

申请号：US12275610

申请日：2008-11-21

Applicant: Tetsumasa Ito ,  Yukimitsu Kijima ,  Koji Fujimoto

Inventor： Tetsumasa Ito ,  Yukimitsu Kijima ,  Koji Fujimoto

IPC: G01N21/00

CPC classification number: G01N1/06 ,  G01N1/36 ,  Y10T436/2575

Abstract: An embedded block is capable of being humidified in a short time without influencing a surface temperature of the embedded block as much as possible. An embedded block humidifier for humidifying an embedded block having a biological specimen embedded in an embedding agent is provided which includes a vapor generating mechanism for generating vapor heated at a predetermined temperature and a guiding mechanism for guiding the vapor to the embedded block so that the generated vapor contacts with a surface of the embedded block set at a predetermined standby position.

Abstract translation: 嵌入块能够在短时间内被加湿，而不会尽可能地影响嵌入块的表面温度。 提供了一种用于加湿嵌入嵌入剂中的具有生物样本的嵌入块的嵌入式块加湿器，其包括用于产生在预定温度下被加热的蒸气的蒸汽发生机构和用于将蒸汽引导到嵌入块的引导机构， 蒸汽与嵌入块的表面设置在预定待机位置。

公开(公告)号：US07966917B2

公开(公告)日：2011-06-28

申请号：US11781386

申请日：2007-07-23

Applicant: Volker Schneider

Inventor： Volker Schneider

IPC: B26D1/00

CPC classification number: G01N1/06 ,  Y10T83/727 ,  Y10T83/929

Abstract: A lever arrangement for a microtome including a first and second lever. The first lever mounted rotatably about a first axis. The second lever is mounted rotatably about a second axis substantially parallel to the first axis. One lever comprises two guidance surfaces. The other lever comprises a guidance segment. The guidance segment coacts with the two guidance surfaces such that a rotation of the first lever in a first direction rotates the second lever in a target direction, and a rotation of the first lever in a direction opposite to the first direction rotates the second lever in the target direction.

Abstract translation: 一种用于切片机的杆装置，包括第一和第二杆。 第一杠杆围绕第一轴线可旋转地安装。 第二杠杆围绕基本平行于第一轴线的第二轴线可旋转地安装。 一个杠杆包括两个引导表面。 另一个杠杆包括引导段。 引导段与两个引导表面共同作用，使得第一杆在第一方向上的旋转沿着目标方向旋转第二杆，并且第一杆在与第一方向相反的方向上的旋转使第二杆旋转 目标方向。

公开(公告)号：US20110030523A1

公开(公告)日：2011-02-10

申请号：US12656860

申请日：2010-02-18

Applicant: Reiner Fank

Inventor： Reiner Fank

IPC: B26D7/22

CPC classification number: G01N1/06 ,  G01N2001/061 ,  Y10T83/606 ,  Y10T83/6508 ,  Y10T83/7734 ,  Y10T83/8723

Abstract: A knife holder device for a microtome having a blade guard constituted as a pivotable bow, wherein, in a protective position, the blade guard is disposed on the side of the knife facing away from the sample at a distance such that access to the cutting edge is not possible. The operability is improved and simplified and therefore safe operation is adequately ensured. This is achieved by constituting the blade guard such that it can be positioned in three different defined positions and is provided with elements that have the following effects depending on the position: in the upper, protective position, simultaneous fixing of the position of the knife holder in the guideway; in the central, knife-holder-sliding position, accessibility to the knife holder with simultaneous release of the position fixture of the knife holder in the guideway; and in the lower, knife-changing position, free accessibility to the knife holder with simultaneous fixture of the position of the knife holder in the guideway. The blade guard and the elements are constituted such that ergonomic removal of sections is only possible in the protective position.

Abstract translation: 一种用于切片机的刀架装置，其具有构成可枢转弓的刀片防护罩，其中，在保护位置，所述刀片防护装置设置在远离所述样品的所述刀的所述侧面上，使得接近所述切削刃 不可能。 可操作性得到改善和简化，因此充分保证了安全运行。 这是通过构成刀片防护罩来实现的，使得其可以被定位在三个不同的限定位置，并且具有根据位置具有以下效果的元件：在上部保护位置中，同时固定刀架的位置 在导轨上 在中央，刀架滑动位置，刀架的可接近性，同时释放刀架在导轨中的位置夹具; 并且在较低的切刀位置，刀片架的自由接近性同时固定刀架在导轨中的位置。 刀片护罩和元件的构造使得仅在保护位置可以进行人体工程学的切割。

公开(公告)号：US20100323445A1

公开(公告)日：2010-12-23

申请号：US12695297

申请日：2010-01-28

Applicant: Kenneth Jeffrey Hayworth ,  Amy Au Hayworth

Inventor： Kenneth Jeffrey Hayworth ,  Amy Au Hayworth

IPC: C12N5/071

CPC classification number: G01N1/06 ,  G01N1/286 ,  G01N1/312 ,  G01N2001/066 ,  G01N2001/362 ,  H01J2237/201 ,  H01J2237/202 ,  H01J2237/26 ,  Y10T156/1052 ,  Y10T156/1056 ,  Y10T156/1057 ,  Y10T156/1059 ,  Y10T156/1062 ,  Y10T156/12 ,  Y10T156/1322 ,  Y10T156/1712

Abstract: Methods, apparatus and systems for collecting thin tissue samples for imaging. Thin tissue sections may be cut from tissue samples using a microtome-quality knife. In one example, tissue samples are mounted to a substrate that is rotated such that thin tissue sections are acquired via lathing. Collection of thin tissue sections may be facilitated by a conveyor belt. Thin tissue sections may be mounted to a thin substrate (e.g., by adhering thin tissue sections to a thin substrate via a roller mechanism) that may be imaged, for example, by an electron beam (e.g., in an electron microscope). This tissue sections may be strengthened before cutting via a blockface thinfilm deposition technique and/or a blockface taping technique. An automated reel-to-reel imaging technique may be employed for collected/mounted tissue sections to facilitate random-access imaging of tissue sections and maintaining a comprehensive library including a large volume of samples.

Abstract translation: 用于收集用于成像的薄组织样本的方法，装置和系统。 可以使用切片机质量刀从组织样品切割薄组织切片。 在一个实例中，将组织样品安装到旋转的基底上，使得通过车床获得薄的组织切片。 通过传送带可以促进薄组织切片的收集。 薄组织切片可以例如通过电子束（例如，在电子显微镜中）被安装到薄基底（例如，通过将薄组织切片通过辊机构粘附到薄基底上）。 在经由块状薄膜沉积技术和/或块状胶带技术切割之前，可以加强该组织切片。 可以采用自动卷线对卷成像技术来收集/安装的组织切片，以促进组织切片的随机取景成像并维持包括大量样品的综合库。

公开(公告)号：US07842236B2

公开(公告)日：2010-11-30

申请号：US11965528

申请日：2007-12-27

Applicant: Wannhsin Chen ,  Lih-Tao Hsu ,  Yeou-Bin Guu ,  Yu Mei Chen

Inventor： Wannhsin Chen ,  Lih-Tao Hsu ,  Yeou-Bin Guu ,  Yu Mei Chen

IPC: G01N21/00

CPC classification number: G01N1/06 ,  C12M33/04 ,  G01N1/2813 ,  Y10T83/263 ,  Y10T83/283

Abstract: A selecting apparatus for selecting cell/tissue mass includes a base, a feeding mechanism and a dividing mechanism. The base has a platform for placing the cell/tissue mass. The feeding mechanism, disposed on the base, moves relative to the platform. The dividing mechanism, disposed on the feeding mechanism, includes a first cutting set and a second cutting set connected therewith. The second cutting set reciprocally moves between a first position and a second position, toward the first cutting set. The feeding mechanism moves the dividing mechanism above the platform, aligning the dividing mechanism with a portion of the cell/tissue mass, and moves the first cutting set toward the cell/tissue mass, cutting into the portion of the cell/tissue mass. The second cutting set moves from the first position to the second position, limiting the portion of the cell/tissue mass between the first cutting set and the second cutting set.

Abstract translation: 用于选择细胞/组织块的选择装置包括基底，进给机构和分割机构。 该基座具有放置细胞/组织块的平台。 设置在基座上的进给机构相对于平台移动。 配置在供给机构上的分割机构包括第一切割组和与其连接的第二切割组。 第二切割组在第一位置和第二位置之间往复移动朝向第一切割组。 供给机构将分割机构移动到平台上方，使分割机构与细胞/组织块的一部分对准，并使第一切割组朝向细胞/组织块移动，切割成细胞/组织块的部分。 第二切割组从第一位置移动到第二位置，限制第一切割组和第二切割组之间的细胞/组织块的部分。

公开(公告)号：US20100175520A1

公开(公告)日：2010-07-15

申请号：US12310519

申请日：2007-05-22

Applicant: Jian-Qiang Kong ,  George Y. Kong

Inventor： Jian-Qiang Kong ,  George Y. Kong

IPC: G01N1/06 ,  B26D1/45

CPC classification number: G01N1/06 ,  Y10T83/04 ,  Y10T83/8874

Abstract: Fresh tissue slices can be cut from tissue specimens embedded in a low gel point agarose by the use of a vibrating, sliding-blade microtome. The microtome has a blade which vibrates in a generally horizontal direction and which is supported for movement in a generally diagonal, sliding direction with respect to the tissue specimen. The compound vibrating and sliding motion of the cutting blade of the microtome is very effective in cutting fresh tissue slices that have a slice viability, measured as a ratio of live to dead cells, which is much better than that which has been obtained using conventional vibrating microtomes.

Abstract translation: 可以通过使用振动的滑动刀片切片机从嵌入在低凝胶点琼脂糖中的组织标本切割新鲜的组织切片。 切片机具有在大致水平方向上振动并且被支撑为相对于组织样本沿大致对角线滑动方向运动的叶片。 切片机的切割刀片的复合振动和滑动运动在切割具有切片存活力的新鲜组织切片方面非常有效，测量为活细胞与死细胞的比例，其比使用常规振动 切片机

公开(公告)号：US20100118133A1

公开(公告)日：2010-05-13

申请号：US12537561

申请日：2009-08-07

Applicant: Roland Walter ,  Andreas Laudat ,  Annett Leonhardt

Inventor： Roland Walter ,  Andreas Laudat ,  Annett Leonhardt

IPC: H04N7/18 ,  G06K9/00

CPC classification number: G01N1/06 ,  G01N35/00594 ,  Y10T83/04 ,  Y10T83/148

Abstract: A method and a device for producing thin sections of a sample by means of a microtome is described, in which a camera acquires at least one image of a surface generated by sectioning of the sample. With the aid of an evaluation device, the image of the surface is evaluated in terms of predefined characteristic values of a section quality. As a function of the characteristic values that are identified, a decision is then made as to whether the section of the sample is accepted or not.

Abstract translation: 描述了一种通过切片机制造样品薄部分的方法和装置，其中照相机获取通过样品切片产生的表面的至少一个图像。 借助于评估装置，根据截面质量的预定特征值来评估表面的图像。 根据识别的特征值的函数，然后作出关于样品的部分是否被接受的决定。

公开(公告)号：US20100058913A1

公开(公告)日：2010-03-11

申请号：US12557456

申请日：2009-09-10

Applicant: Roland Walter

Inventor： Roland Walter

IPC: G01N1/06 ,  B26D7/01

CPC classification number: G01N1/06 ,  Y10T83/6568 ,  Y10T83/889

Abstract: A microtome is described, having a cassette changing apparatus that comprises a stationary guide bar. The latter guides a second slider that is a guide for the first slider. The first and the second slider are coupled to one another in such a way that a movement of the second slider along the stationary guide bar operates a movement of the first slider along with the second slider and additionally relative to the second slider.

Abstract translation: 描述了一种切片机，其具有包括固定导杆的盒式磁带更换装置。 后者引导作为第一滑块的引导件的第二滑块。 第一滑动器和第二滑块彼此联接，使得第二滑块沿着固定引导杆的运动使第一滑块与第二滑块一起运动，另外还相对于第二滑动件运动。